Ispitivanja novih biosenzora za monitoring i dijagnostiku biljaka

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Ispitivanja novih biosenzora za monitoring i dijagnostiku biljaka (en)
Испитивања нових биосензора за мониторинг и дијагностику биљака (sr)
Ispitivanja novih biosenzora za monitoring i dijagnostiku biljaka (sr_RS)
Authors

Publications

The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model

Laketa, Danijela; Bogdanović, Jelena; Prodanović, Radivoje; Kalauzi, Aleksandar; Radotić, Ksenija

(General Physiol And Biophysics, Bratislava, 2010)

TY  - JOUR
AU  - Laketa, Danijela
AU  - Bogdanović, Jelena
AU  - Prodanović, Radivoje
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2010
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1089
AB  - We studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active.
PB  - General Physiol And Biophysics, Bratislava
T2  - General Physiology and Biophysics
T1  - The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model
VL  - 29
IS  - 2
SP  - 122
EP  - 128
DO  - 10.4149/gpb_2010_02_122
ER  - 
@article{
author = "Laketa, Danijela and Bogdanović, Jelena and Prodanović, Radivoje and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2010",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1089",
abstract = "We studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active.",
publisher = "General Physiol And Biophysics, Bratislava",
journal = "General Physiology and Biophysics",
title = "The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model",
volume = "29",
number = "2",
pages = "122-128",
doi = "10.4149/gpb_2010_02_122"
}
Laketa, D., Bogdanović, J., Prodanović, R., Kalauzi, A.,& Radotić, K. (2010). The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model.
General Physiology and BiophysicsGeneral Physiol And Biophysics, Bratislava., 29(2), 122-128.
https://doi.org/10.4149/gpb_2010_02_122
Laketa D, Bogdanović J, Prodanović R, Kalauzi A, Radotić K. The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model. General Physiology and Biophysics. 2010;29(2):122-128
Laketa Danijela, Bogdanović Jelena, Prodanović Radivoje, Kalauzi Aleksandar, Radotić Ksenija, "The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model" 29, no. 2 (2010):122-128,
https://doi.org/10.4149/gpb_2010_02_122 .

Antioxidative enzymes during germination of two lines of Serbian spruce [picea omorika (panc.) purkyae]

Prodanović, Olivera; Prodanović, Radivoje; Bogdanović, Jelena; Mitrović, Aleksandra D.; Niilosavic, N.; Radotić, Ksenija

(Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd, 2007)

TY  - JOUR
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
AU  - Bogdanović, Jelena
AU  - Mitrović, Aleksandra D.
AU  - Niilosavic, N.
AU  - Radotić, Ksenija
PY  - 2007
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/928
AB  - dTwo lines of Picea omorika (Pane.) Purkyne were compared with respect to germination percentage as well as specific activity and isoenzyme pattern of catalase, superoxide dismutase, and peroxidase (POD) during germination. Line A had a higher gennination percentage and higher enzyme activities in dry seeds and seedlings compared to line C. Peroxidase activity was not detected in dry seeds, but measured up to 10 U/g and 28 U/g on the 7(th) day of germination in lines C and A, respectively. The most abundant POD basic isoform in seedlings of both lines (pl 8.2) was not found previously in needles of adult Serbian spruce trees of the same lines.
AB  - Poređeni su procenat klijanja, specifična aktivnost i izoenzimski profil katalaze, superoksid dismutaze i peroksidaze u dve linije omorike [Picea omorika (Panč.) Purkyně] u toku klijanja. Linija A je imala veći procenat klijanja i veće enzimske aktivnosti u suvom semenu i klijancima, u poređenju sa linijom C. Peroksidazna aktivnost nije nađena u suvim semenima, a povećavala se do 10 U/g i 28 U/g sed- mog dana klijanja u linijama C i A, respektivno. Najzastupljenija bazna izoforma peroksidaze u klijancima obe linije (pI 8.2) nije nađena ranije u četinama odraslih jedinki istih linija. .
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Antioxidative enzymes during germination of two lines of Serbian spruce [picea omorika (panc.) purkyae]
T1  - Enzimi zaštite od oksidacionih oštećenja u toku klijanja dve linije omorike [Picea omorika (Panč.) Purkyně]
VL  - 59
IS  - 3
SP  - 209
EP  - 216
DO  - 10.2298/ABS0703209P
ER  - 
@article{
author = "Prodanović, Olivera and Prodanović, Radivoje and Bogdanović, Jelena and Mitrović, Aleksandra D. and Niilosavic, N. and Radotić, Ksenija",
year = "2007",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/928",
abstract = "dTwo lines of Picea omorika (Pane.) Purkyne were compared with respect to germination percentage as well as specific activity and isoenzyme pattern of catalase, superoxide dismutase, and peroxidase (POD) during germination. Line A had a higher gennination percentage and higher enzyme activities in dry seeds and seedlings compared to line C. Peroxidase activity was not detected in dry seeds, but measured up to 10 U/g and 28 U/g on the 7(th) day of germination in lines C and A, respectively. The most abundant POD basic isoform in seedlings of both lines (pl 8.2) was not found previously in needles of adult Serbian spruce trees of the same lines., Poređeni su procenat klijanja, specifična aktivnost i izoenzimski profil katalaze, superoksid dismutaze i peroksidaze u dve linije omorike [Picea omorika (Panč.) Purkyně] u toku klijanja. Linija A je imala veći procenat klijanja i veće enzimske aktivnosti u suvom semenu i klijancima, u poređenju sa linijom C. Peroksidazna aktivnost nije nađena u suvim semenima, a povećavala se do 10 U/g i 28 U/g sed- mog dana klijanja u linijama C i A, respektivno. Najzastupljenija bazna izoforma peroksidaze u klijancima obe linije (pI 8.2) nije nađena ranije u četinama odraslih jedinki istih linija. .",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Antioxidative enzymes during germination of two lines of Serbian spruce [picea omorika (panc.) purkyae], Enzimi zaštite od oksidacionih oštećenja u toku klijanja dve linije omorike [Picea omorika (Panč.) Purkyně]",
volume = "59",
number = "3",
pages = "209-216",
doi = "10.2298/ABS0703209P"
}
Prodanović, O., Prodanović, R., Bogdanović, J., Mitrović, A. D., Niilosavic, N.,& Radotić, K. (2007). Enzimi zaštite od oksidacionih oštećenja u toku klijanja dve linije omorike [Picea omorika (Panč.) Purkyně].
Archives of biological sciencesInst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 59(3), 209-216.
https://doi.org/10.2298/ABS0703209P
Prodanović O, Prodanović R, Bogdanović J, Mitrović AD, Niilosavic N, Radotić K. Enzimi zaštite od oksidacionih oštećenja u toku klijanja dve linije omorike [Picea omorika (Panč.) Purkyně]. Archives of biological sciences. 2007;59(3):209-216
Prodanović Olivera, Prodanović Radivoje, Bogdanović Jelena, Mitrović Aleksandra D., Niilosavic N., Radotić Ksenija, "Enzimi zaštite od oksidacionih oštećenja u toku klijanja dve linije omorike [Picea omorika (Panč.) Purkyně]" 59, no. 3 (2007):209-216,
https://doi.org/10.2298/ABS0703209P .
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Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)

Bogdanović, Jelena; Milosavić, Nenad; Prodanović, Radivoje; Ducic, Tanja; Radotić, Ksenija

(Pergamon-Elsevier Science Ltd, Oxford, 2007)

TY  - JOUR
AU  - Bogdanović, Jelena
AU  - Milosavić, Nenad
AU  - Prodanović, Radivoje
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2007
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/829
AB  - Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones. (C) 2006 Elsevier Ltd. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Biochemical Systematics and Ecology
T1  - Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)
VL  - 35
IS  - 5
SP  - 263
EP  - 273
DO  - 10.1016/j.bse.2006.12.001
ER  - 
@article{
author = "Bogdanović, Jelena and Milosavić, Nenad and Prodanović, Radivoje and Ducic, Tanja and Radotić, Ksenija",
year = "2007",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/829",
abstract = "Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones. (C) 2006 Elsevier Ltd. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Biochemical Systematics and Ecology",
title = "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)",
volume = "35",
number = "5",
pages = "263-273",
doi = "10.1016/j.bse.2006.12.001"
}
Bogdanović, J., Milosavić, N., Prodanović, R., Ducic, T.,& Radotić, K. (2007). Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye).
Biochemical Systematics and EcologyPergamon-Elsevier Science Ltd, Oxford., 35(5), 263-273.
https://doi.org/10.1016/j.bse.2006.12.001
Bogdanović J, Milosavić N, Prodanović R, Ducic T, Radotić K. Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). Biochemical Systematics and Ecology. 2007;35(5):263-273
Bogdanović Jelena, Milosavić Nenad, Prodanović Radivoje, Ducic Tanja, Radotić Ksenija, "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)" 35, no. 5 (2007):263-273,
https://doi.org/10.1016/j.bse.2006.12.001 .
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18
17

Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]

Bogdanović, Jelena; Prodanović, Radivoje; Milosavić, Nenad; Prodanović, Olivera; Radotić, Ksenija

(2006)

TY  - JOUR
AU  - Bogdanović, Jelena
AU  - Prodanović, Radivoje
AU  - Milosavić, Nenad
AU  - Prodanović, Olivera
AU  - Radotić, Ksenija
PY  - 2006
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/237
AB  - Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount.
AB  - Proučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji.
T2  - Archives of biological sciences
T1  - Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]
T1  - Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]
VL  - 58
IS  - 4
SP  - 211
EP  - 214
DO  - 10.2298/ABS0604211B
ER  - 
@article{
author = "Bogdanović, Jelena and Prodanović, Radivoje and Milosavić, Nenad and Prodanović, Olivera and Radotić, Ksenija",
year = "2006",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/237",
abstract = "Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount., Proučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji.",
journal = "Archives of biological sciences",
title = "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně], Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]",
volume = "58",
number = "4",
pages = "211-214",
doi = "10.2298/ABS0604211B"
}
Bogdanović, J., Prodanović, R., Milosavić, N., Prodanović, O.,& Radotić, K. (2006). Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně].
Archives of biological sciences, 58(4), 211-214.
https://doi.org/10.2298/ABS0604211B
Bogdanović J, Prodanović R, Milosavić N, Prodanović O, Radotić K. Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]. Archives of biological sciences. 2006;58(4):211-214
Bogdanović Jelena, Prodanović Radivoje, Milosavić Nenad, Prodanović Olivera, Radotić Ksenija, "Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]" 58, no. 4 (2006):211-214,
https://doi.org/10.2298/ABS0604211B .
4