TY  - JOUR
AU  - Sajewicz, Mieczyslaw
AU  - Staszek, Dorota
AU  - Natić, Maja
AU  - Wojtal, Lukasz
AU  - Waksmundzka-Hajnos, Monika
AU  - Kowalska, Teresa
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1348
AB  - In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separations were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography and Related Technologies
T1  - Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids
VL  - 34
IS  - 10-11
SP  - 864
EP  - 887
DO  - 10.1080/10826076.2011.571131
ER  - 

@article{
author = "Sajewicz, Mieczyslaw and Staszek, Dorota and Natić, Maja and Wojtal, Lukasz and Waksmundzka-Hajnos, Monika and Kowalska, Teresa",
year = "2011",
abstract = "In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separations were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography and Related Technologies",
title = "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids",
volume = "34",
number = "10-11",
pages = "864-887",
doi = "10.1080/10826076.2011.571131"
}

Sajewicz, M., Staszek, D., Natić, M., Wojtal, L., Waksmundzka-Hajnos, M.,& Kowalska, T.. (2011). Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids. in Journal of Liquid Chromatography and Related Technologies
Taylor & Francis Inc, Philadelphia., 34(10-11), 864-887.
https://doi.org/10.1080/10826076.2011.571131

Sajewicz M, Staszek D, Natić M, Wojtal L, Waksmundzka-Hajnos M, Kowalska T. Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids. in Journal of Liquid Chromatography and Related Technologies. 2011;34(10-11):864-887.
doi:10.1080/10826076.2011.571131 .

Sajewicz, Mieczyslaw, Staszek, Dorota, Natić, Maja, Wojtal, Lukasz, Waksmundzka-Hajnos, Monika, Kowalska, Teresa, "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids" in Journal of Liquid Chromatography and Related Technologies, 34, no. 10-11 (2011):864-887,
https://doi.org/10.1080/10826076.2011.571131 . .

TY  - JOUR
AU  - Sajewicz, Mieczyslaw
AU  - Wojtal, Lukasz
AU  - Natić, Maja
AU  - Staszek, Dorota
AU  - Waksmundzka-Hajnos, Monika
AU  - Kowalska, Teresa
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1347
AB  - In our earlier studies, we proposed the low-temperature thin-layer chromatography with densitometric and mass spectrometric detection for fingerprinting of essential oils derived from several different species from the Salvia genus. Development of the proposed LT-TLC-MS approach was only possible with use of the TLC-MS interface able to couple the chromatographic plate with mass spectrometer. The goal of this study was to develop for the same purpose the two-dimensional liquid chromatographic system, which was only possible with use of the TLC-MS interface. It included the low-temperature thin-layer chromatography coupled with high-performance liquid chromatography, to build an overall LT TLC-LC-MS system. We compared the fingerprinting results for the essential oil of Salvia lavandulifolia obtained with use of the one-dimensional system (1D LT TLC-MS) with those obtained with use of the two-dimensional system (2D LT TLC-LC-MS). It was shown that the 2D approach provides more fingerprints for further investigations than the 1D approach. Moreover, it is also clear that the 2D approach is better suited for identification of individual chemical species contained in a given mixture, due to the simpler patterns of mass spectra obtained from the 2D analytical mode than from the 1D one. However, with the constituents of the essential oil derived from Salvia lavandulifolia, a straightforward identification was not possible, basically due to numerous groups of isomers among the terpenes and terpenoids contained in the examined sample that have the same molecular weights and hence, can give identical m/z signals.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography and Related Technologies
T1  - Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part I. Essential Oils
VL  - 34
IS  - 10-11
SP  - 848
EP  - 863
DO  - 10.1080/10826076.2011.571136
ER  - 

@article{
author = "Sajewicz, Mieczyslaw and Wojtal, Lukasz and Natić, Maja and Staszek, Dorota and Waksmundzka-Hajnos, Monika and Kowalska, Teresa",
year = "2011",
abstract = "In our earlier studies, we proposed the low-temperature thin-layer chromatography with densitometric and mass spectrometric detection for fingerprinting of essential oils derived from several different species from the Salvia genus. Development of the proposed LT-TLC-MS approach was only possible with use of the TLC-MS interface able to couple the chromatographic plate with mass spectrometer. The goal of this study was to develop for the same purpose the two-dimensional liquid chromatographic system, which was only possible with use of the TLC-MS interface. It included the low-temperature thin-layer chromatography coupled with high-performance liquid chromatography, to build an overall LT TLC-LC-MS system. We compared the fingerprinting results for the essential oil of Salvia lavandulifolia obtained with use of the one-dimensional system (1D LT TLC-MS) with those obtained with use of the two-dimensional system (2D LT TLC-LC-MS). It was shown that the 2D approach provides more fingerprints for further investigations than the 1D approach. Moreover, it is also clear that the 2D approach is better suited for identification of individual chemical species contained in a given mixture, due to the simpler patterns of mass spectra obtained from the 2D analytical mode than from the 1D one. However, with the constituents of the essential oil derived from Salvia lavandulifolia, a straightforward identification was not possible, basically due to numerous groups of isomers among the terpenes and terpenoids contained in the examined sample that have the same molecular weights and hence, can give identical m/z signals.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography and Related Technologies",
title = "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part I. Essential Oils",
volume = "34",
number = "10-11",
pages = "848-863",
doi = "10.1080/10826076.2011.571136"
}

Sajewicz, M., Wojtal, L., Natić, M., Staszek, D., Waksmundzka-Hajnos, M.,& Kowalska, T.. (2011). Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part I. Essential Oils. in Journal of Liquid Chromatography and Related Technologies
Taylor & Francis Inc, Philadelphia., 34(10-11), 848-863.
https://doi.org/10.1080/10826076.2011.571136

Sajewicz M, Wojtal L, Natić M, Staszek D, Waksmundzka-Hajnos M, Kowalska T. Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part I. Essential Oils. in Journal of Liquid Chromatography and Related Technologies. 2011;34(10-11):848-863.
doi:10.1080/10826076.2011.571136 .

Sajewicz, Mieczyslaw, Wojtal, Lukasz, Natić, Maja, Staszek, Dorota, Waksmundzka-Hajnos, Monika, Kowalska, Teresa, "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part I. Essential Oils" in Journal of Liquid Chromatography and Related Technologies, 34, no. 10-11 (2011):848-863,
https://doi.org/10.1080/10826076.2011.571136 . .