TY  - JOUR
AU  - Mančić, Lidija
AU  - Đukić-Vuković, Aleksandra
AU  - Dinić, Ivana
AU  - Nikolić, Marko G.
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar J.
AU  - Costa, Antonio M. L. M.
AU  - Marinkovic, Bojan A.
AU  - Mojović, Ljiljana
AU  - Milošević, Olivera B.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2225
AB  - The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt  (4)/(15/2)) and stronger red emission (F-4(9/2) - gt  (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt  (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging
VL  - 8
IS  - 48
SP  - 27429
EP  - 27437
DO  - 10.1039/c8ra04178d
ER  - 

@article{
author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar J. and Costa, Antonio M. L. M. and Marinkovic, Bojan A. and Mojović, Ljiljana and Milošević, Olivera B.",
year = "2018",
abstract = "The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt  (4)/(15/2)) and stronger red emission (F-4(9/2) - gt  (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt  (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging",
volume = "8",
number = "48",
pages = "27429-27437",
doi = "10.1039/c8ra04178d"
}

Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A. J., Costa, A. M. L. M., Marinkovic, B. A., Mojović, L.,& Milošević, O. B.. (2018). One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances
Royal Soc Chemistry, Cambridge., 8(48), 27429-27437.
https://doi.org/10.1039/c8ra04178d

Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot AJ, Costa AMLM, Marinkovic BA, Mojović L, Milošević OB. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances. 2018;8(48):27429-27437.
doi:10.1039/c8ra04178d .

Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar J., Costa, Antonio M. L. M., Marinkovic, Bojan A., Mojović, Ljiljana, Milošević, Olivera B., "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging" in RSC Advances, 8, no. 48 (2018):27429-27437,
https://doi.org/10.1039/c8ra04178d . .

TY  - DATA
AU  - Mančić, Lidija
AU  - Đukić-Vuković, Aleksandra
AU  - Dinić, Ivana
AU  - Nikolić, Marko G.
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar J.
AU  - Costa, Antonio M. L. M.
AU  - Marinkovic, Bojan A.
AU  - Mojović, Ljiljana
AU  - Milošević, Olivera
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3132
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Supplementary data for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.; Costa, A. M. L. M.; Marinkovic, B. A.; Mojovic, L.; Milosevic, O. One-Step Synthesis of Amino-Functionalized up-Converting NaYF 4 :Yb,Er Nanoparticles for: In Vitro Cell Imaging. RSC Advances 2018, 8 (48), 27429–27437. https://doi.org/10.1039/c8ra04178d
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3132
ER  - 

@misc{
author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar J. and Costa, Antonio M. L. M. and Marinkovic, Bojan A. and Mojović, Ljiljana and Milošević, Olivera",
year = "2018",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Supplementary data for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.; Costa, A. M. L. M.; Marinkovic, B. A.; Mojovic, L.; Milosevic, O. One-Step Synthesis of Amino-Functionalized up-Converting NaYF 4 :Yb,Er Nanoparticles for: In Vitro Cell Imaging. RSC Advances 2018, 8 (48), 27429–27437. https://doi.org/10.1039/c8ra04178d",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3132"
}

Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A. J., Costa, A. M. L. M., Marinkovic, B. A., Mojović, L.,& Milošević, O.. (2018). Supplementary data for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.; Costa, A. M. L. M.; Marinkovic, B. A.; Mojovic, L.; Milosevic, O. One-Step Synthesis of Amino-Functionalized up-Converting NaYF 4 :Yb,Er Nanoparticles for: In Vitro Cell Imaging. RSC Advances 2018, 8 (48), 27429–27437. https://doi.org/10.1039/c8ra04178d. in RSC Advances
Royal Soc Chemistry, Cambridge..
https://hdl.handle.net/21.15107/rcub_cherry_3132

Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot AJ, Costa AMLM, Marinkovic BA, Mojović L, Milošević O. Supplementary data for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.; Costa, A. M. L. M.; Marinkovic, B. A.; Mojovic, L.; Milosevic, O. One-Step Synthesis of Amino-Functionalized up-Converting NaYF 4 :Yb,Er Nanoparticles for: In Vitro Cell Imaging. RSC Advances 2018, 8 (48), 27429–27437. https://doi.org/10.1039/c8ra04178d. in RSC Advances. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3132 .

Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar J., Costa, Antonio M. L. M., Marinkovic, Bojan A., Mojović, Ljiljana, Milošević, Olivera, "Supplementary data for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.; Costa, A. M. L. M.; Marinkovic, B. A.; Mojovic, L.; Milosevic, O. One-Step Synthesis of Amino-Functionalized up-Converting NaYF 4 :Yb,Er Nanoparticles for: In Vitro Cell Imaging. RSC Advances 2018, 8 (48), 27429–27437. https://doi.org/10.1039/c8ra04178d" in RSC Advances (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3132 .