TY  - CONF
AU  - Gnjatović, Marija Lj.
AU  - Đukić, Teodora
AU  - Stanić-Vučinić, Dragana
AU  - Radosavljević, Jelena
AU  - Smiljanić, Katarina
AU  - Vasović, Tamara
AU  - Simović, Ana
AU  - Mladenović, Maja
AU  - Radomirović, Mirjana Ž.
AU  - Ćirković-Veličković, Tanja
AU  - Ćujić, Danica R.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5767
AB  - The COVID-19 diagnostic tools are categorized into two main groups of Nucleic Acid (NA)-
based and protein-based tests. To date, nucleic acid-based detection has been announced as the
gold-standard strategy for coronavirus detection; however, protein-based tests are promising
alternatives for rapid and large-scale screening of susceptible groups. During the first months, no
rapid and reliable detecting tool was readily available to adequatly respond to the requirement of
massive testing. The aim was to develope cost-effective, sensitive and rapid screening
mechanisms for the detection of immune response to SARS-CoV2 (which causes COVID-19),
based on the principle of ELISA. The institute INEP has developed tests for detection of IgM
and IgG SARS-CoV-2 specific antibodies based on S and N proteins of virus intended to monitor
different phases of natural infection, ELISA test for IgG detection in naturale infection based on
the use of exclusively domestic components of the ELISA kit (including proteins produced in
Serbia, Faculty of Chemistry) and a test specifically designed to monitor the effects of
immunization (determination of IgG antibodies specific for the RBD domain of S protein). The
tests were independently validated at the relevant laboratories in the country and abroad, and
compared to an FDA/WHO approved tests of a major test producers. All testing for validation
was carried out on samples collected before COVID19 (negative controls), PCR confirmed
COVID19 samples (positive controls) and potencialy cross-reactive samples (other pathogens
and autoimmune diseases). The antibody tests showed high levels of sensitivity and specificity
and extremely low background noises. The kits are extremely stable, have a shelf life of 1 year
and opened kits are usable up to 3 months at storing temperatures of 5°C
PB  - Univerzitet u Beogradu - Hemijski fakultet
C3  - Book of Abstracts of the 3rd Workshop FoodenTwin 2021, Belgrade 15 June
T1  - Serological Elisa test development at the INEP Institute
SP  - 18
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5767
ER  - 

@conference{
author = "Gnjatović, Marija Lj. and Đukić, Teodora and Stanić-Vučinić, Dragana and Radosavljević, Jelena and Smiljanić, Katarina and Vasović, Tamara and Simović, Ana and Mladenović, Maja and Radomirović, Mirjana Ž. and Ćirković-Veličković, Tanja and Ćujić, Danica R.",
year = "2021",
abstract = "The COVID-19 diagnostic tools are categorized into two main groups of Nucleic Acid (NA)-
based and protein-based tests. To date, nucleic acid-based detection has been announced as the
gold-standard strategy for coronavirus detection; however, protein-based tests are promising
alternatives for rapid and large-scale screening of susceptible groups. During the first months, no
rapid and reliable detecting tool was readily available to adequatly respond to the requirement of
massive testing. The aim was to develope cost-effective, sensitive and rapid screening
mechanisms for the detection of immune response to SARS-CoV2 (which causes COVID-19),
based on the principle of ELISA. The institute INEP has developed tests for detection of IgM
and IgG SARS-CoV-2 specific antibodies based on S and N proteins of virus intended to monitor
different phases of natural infection, ELISA test for IgG detection in naturale infection based on
the use of exclusively domestic components of the ELISA kit (including proteins produced in
Serbia, Faculty of Chemistry) and a test specifically designed to monitor the effects of
immunization (determination of IgG antibodies specific for the RBD domain of S protein). The
tests were independently validated at the relevant laboratories in the country and abroad, and
compared to an FDA/WHO approved tests of a major test producers. All testing for validation
was carried out on samples collected before COVID19 (negative controls), PCR confirmed
COVID19 samples (positive controls) and potencialy cross-reactive samples (other pathogens
and autoimmune diseases). The antibody tests showed high levels of sensitivity and specificity
and extremely low background noises. The kits are extremely stable, have a shelf life of 1 year
and opened kits are usable up to 3 months at storing temperatures of 5°C",
publisher = "Univerzitet u Beogradu - Hemijski fakultet",
journal = "Book of Abstracts of the 3rd Workshop FoodenTwin 2021, Belgrade 15 June",
title = "Serological Elisa test development at the INEP Institute",
pages = "18-18",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5767"
}

Gnjatović, M. Lj., Đukić, T., Stanić-Vučinić, D., Radosavljević, J., Smiljanić, K., Vasović, T., Simović, A., Mladenović, M., Radomirović, M. Ž., Ćirković-Veličković, T.,& Ćujić, D. R.. (2021). Serological Elisa test development at the INEP Institute. in Book of Abstracts of the 3rd Workshop FoodenTwin 2021, Belgrade 15 June
Univerzitet u Beogradu - Hemijski fakultet., 18-18.
https://hdl.handle.net/21.15107/rcub_cherry_5767

Gnjatović ML, Đukić T, Stanić-Vučinić D, Radosavljević J, Smiljanić K, Vasović T, Simović A, Mladenović M, Radomirović MŽ, Ćirković-Veličković T, Ćujić DR. Serological Elisa test development at the INEP Institute. in Book of Abstracts of the 3rd Workshop FoodenTwin 2021, Belgrade 15 June. 2021;:18-18.
https://hdl.handle.net/21.15107/rcub_cherry_5767 .

Gnjatović, Marija Lj., Đukić, Teodora, Stanić-Vučinić, Dragana, Radosavljević, Jelena, Smiljanić, Katarina, Vasović, Tamara, Simović, Ana, Mladenović, Maja, Radomirović, Mirjana Ž., Ćirković-Veličković, Tanja, Ćujić, Danica R., "Serological Elisa test development at the INEP Institute" in Book of Abstracts of the 3rd Workshop FoodenTwin 2021, Belgrade 15 June (2021):18-18,
https://hdl.handle.net/21.15107/rcub_cherry_5767 .