Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry)

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Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200168 (Univerzitet u Beogradu, Hemijski fakultet) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200168 (Универзитет у Београду, Хемијски факултет) (sr)
Authors

Publications

Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating

Radomirović, Mirjana; Minić, Simeon; Stanić-Vučinić, Dragana; Nikolić, Milan; Van Haute, Sam; Rajković, Andreja; Ćirković-Veličković, Tanja

(Elsevier, 2022)

TY  - JOUR
AU  - Radomirović, Mirjana
AU  - Minić, Simeon
AU  - Stanić-Vučinić, Dragana
AU  - Nikolić, Milan
AU  - Van Haute, Sam
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4863
AB  - β-lactoglobulin (BLG) is a major whey protein with numerous techno-functional properties desirable for the food industry. Phycocyanobilin (PCB), a bioactive pigment of Arthrospira platensis with health-promoting effects, covalently binds to BLG at physiological pH. This study investigated the effects of this covalent modification on BLG functional properties. The BLG–PCB adduct possesses enhanced antioxidant properties, and bound PCB protects BLG against free radical-induced oxidation. Despite the similar thermal stabilities of BLG and BLG–PCB, BLG–PCB is less susceptible to covalent and noncovalent aggregation under moderate heat treatment (63 °C, 30 min). Blocked thiol group and reduced hydrophobicity due to hindering of hydrophobic residues by bound PCB, as well as the heat-induced transition of β-sheet to α-helix, contributed to the low susceptibility of BLG–PCB to aggregation. BLG–PCB has a higher resistance to pepsin and pancreatin digestion than BLG and unaltered IgE-binding properties. The improved functional properties of BLG–PCB make it a useful ingredient in the food industry.
PB  - Elsevier
T2  - Food Hydrocolloids
T1  - Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating
VL  - 123
SP  - 107169
DO  - 10.1016/j.foodhyd.2021.107169
ER  - 
@article{
author = "Radomirović, Mirjana and Minić, Simeon and Stanić-Vučinić, Dragana and Nikolić, Milan and Van Haute, Sam and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2022",
abstract = "β-lactoglobulin (BLG) is a major whey protein with numerous techno-functional properties desirable for the food industry. Phycocyanobilin (PCB), a bioactive pigment of Arthrospira platensis with health-promoting effects, covalently binds to BLG at physiological pH. This study investigated the effects of this covalent modification on BLG functional properties. The BLG–PCB adduct possesses enhanced antioxidant properties, and bound PCB protects BLG against free radical-induced oxidation. Despite the similar thermal stabilities of BLG and BLG–PCB, BLG–PCB is less susceptible to covalent and noncovalent aggregation under moderate heat treatment (63 °C, 30 min). Blocked thiol group and reduced hydrophobicity due to hindering of hydrophobic residues by bound PCB, as well as the heat-induced transition of β-sheet to α-helix, contributed to the low susceptibility of BLG–PCB to aggregation. BLG–PCB has a higher resistance to pepsin and pancreatin digestion than BLG and unaltered IgE-binding properties. The improved functional properties of BLG–PCB make it a useful ingredient in the food industry.",
publisher = "Elsevier",
journal = "Food Hydrocolloids",
title = "Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating",
volume = "123",
pages = "107169",
doi = "10.1016/j.foodhyd.2021.107169"
}
Radomirović, M., Minić, S., Stanić-Vučinić, D., Nikolić, M., Van Haute, S., Rajković, A.,& Ćirković-Veličković, T.. (2022). Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating. in Food Hydrocolloids
Elsevier., 123, 107169.
https://doi.org/10.1016/j.foodhyd.2021.107169
Radomirović M, Minić S, Stanić-Vučinić D, Nikolić M, Van Haute S, Rajković A, Ćirković-Veličković T. Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating. in Food Hydrocolloids. 2022;123:107169.
doi:10.1016/j.foodhyd.2021.107169 .
Radomirović, Mirjana, Minić, Simeon, Stanić-Vučinić, Dragana, Nikolić, Milan, Van Haute, Sam, Rajković, Andreja, Ćirković-Veličković, Tanja, "Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating" in Food Hydrocolloids, 123 (2022):107169,
https://doi.org/10.1016/j.foodhyd.2021.107169 . .
2

Novel derivatives of aroylacrylic acid phenylamides as inducers of apoptosis through the ROS-mediated pathway in several cancer cell lines

Vujatović, Tamara B.; Vitorović-Todorović, Maja D.; Cvijetić, Ilija; Vasović, Tamara; Nikolić, Milan; Novaković, Irena T.; Bjelogrlić, Snežana K.

(Elsevier, 2022)

TY  - JOUR
AU  - Vujatović, Tamara B.
AU  - Vitorović-Todorović, Maja D.
AU  - Cvijetić, Ilija
AU  - Vasović, Tamara
AU  - Nikolić, Milan
AU  - Novaković, Irena T.
AU  - Bjelogrlić, Snežana K.
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S0022286021018287
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4761
AB  - In the present work, the α,β-double bond of the aroylacrylic acid phenylamides was suitably modified to optimize the toxicity–antiproliferative activity ratio of the resulting compounds 1–5. The phenylamides were modified by Michael's addition of suitably chosen piperidine-containing fragments: 1-amino-N-benzylpiperidine (a1), 4-benzylpiperidine (a2), and N,N-dimethyl-N-[2-(1-piperazinyl)-ethyl]amine (a3). The compounds exerted micromolar activity toward three cancer cell lines, A549, LoVo, and Skov-3, causing apoptotic cell death. It was shown that the nature of the cyclic amine moiety at position C2 of the compounds is probably the primary determinant of anticancer activity toward tested cell lines and the acute toxicity toward brine shrimp (Artemia salina). The majority of compounds revealed the ability to vigorously induce mitochondrial superoxide anion generation in all treated cell lines, which together with cell cycle arrest at the S phase and activation of intrinsic caspase cascade, indicates the possibility that apoptosis was triggered due to irreparable chromosomal damage by acute oxidative stress. Two derivatives also exerted significant antibacterial activity with one order of magnitude higher potency than chloramphenicol in most of the investigated bacterial strains. Also, the drug-like properties for all compounds were estimated by available software tools.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Novel derivatives of aroylacrylic acid phenylamides as inducers of apoptosis through the ROS-mediated pathway in several cancer cell lines
VL  - 1250
SP  - 131702
DO  - 10.1016/j.molstruc.2021.131702
ER  - 
@article{
author = "Vujatović, Tamara B. and Vitorović-Todorović, Maja D. and Cvijetić, Ilija and Vasović, Tamara and Nikolić, Milan and Novaković, Irena T. and Bjelogrlić, Snežana K.",
year = "2022",
abstract = "In the present work, the α,β-double bond of the aroylacrylic acid phenylamides was suitably modified to optimize the toxicity–antiproliferative activity ratio of the resulting compounds 1–5. The phenylamides were modified by Michael's addition of suitably chosen piperidine-containing fragments: 1-amino-N-benzylpiperidine (a1), 4-benzylpiperidine (a2), and N,N-dimethyl-N-[2-(1-piperazinyl)-ethyl]amine (a3). The compounds exerted micromolar activity toward three cancer cell lines, A549, LoVo, and Skov-3, causing apoptotic cell death. It was shown that the nature of the cyclic amine moiety at position C2 of the compounds is probably the primary determinant of anticancer activity toward tested cell lines and the acute toxicity toward brine shrimp (Artemia salina). The majority of compounds revealed the ability to vigorously induce mitochondrial superoxide anion generation in all treated cell lines, which together with cell cycle arrest at the S phase and activation of intrinsic caspase cascade, indicates the possibility that apoptosis was triggered due to irreparable chromosomal damage by acute oxidative stress. Two derivatives also exerted significant antibacterial activity with one order of magnitude higher potency than chloramphenicol in most of the investigated bacterial strains. Also, the drug-like properties for all compounds were estimated by available software tools.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Novel derivatives of aroylacrylic acid phenylamides as inducers of apoptosis through the ROS-mediated pathway in several cancer cell lines",
volume = "1250",
pages = "131702",
doi = "10.1016/j.molstruc.2021.131702"
}
Vujatović, T. B., Vitorović-Todorović, M. D., Cvijetić, I., Vasović, T., Nikolić, M., Novaković, I. T.,& Bjelogrlić, S. K.. (2022). Novel derivatives of aroylacrylic acid phenylamides as inducers of apoptosis through the ROS-mediated pathway in several cancer cell lines. in Journal of Molecular Structure
Elsevier., 1250, 131702.
https://doi.org/10.1016/j.molstruc.2021.131702
Vujatović TB, Vitorović-Todorović MD, Cvijetić I, Vasović T, Nikolić M, Novaković IT, Bjelogrlić SK. Novel derivatives of aroylacrylic acid phenylamides as inducers of apoptosis through the ROS-mediated pathway in several cancer cell lines. in Journal of Molecular Structure. 2022;1250:131702.
doi:10.1016/j.molstruc.2021.131702 .
Vujatović, Tamara B., Vitorović-Todorović, Maja D., Cvijetić, Ilija, Vasović, Tamara, Nikolić, Milan, Novaković, Irena T., Bjelogrlić, Snežana K., "Novel derivatives of aroylacrylic acid phenylamides as inducers of apoptosis through the ROS-mediated pathway in several cancer cell lines" in Journal of Molecular Structure, 1250 (2022):131702,
https://doi.org/10.1016/j.molstruc.2021.131702 . .

CCDC 2110388: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"

Pevec, Andrej

(The Cambridge Crystallographic Data Centre (CCDC), 2022)

TY  - DATA
AU  - Pevec, Andrej
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4861
AB  - NAMYUZ : diazido-[2-oxy-N,N,N-trimethyl-2-{[1-(1,3-thiazol-2-yl)ethylidene]hydrazinylidene}ethan-1-aminium]-zinc Space Group: P 21/c (14), Cell: a 13.0826(10)Å b 10.2506(7)Å c 13.1685(13)Å, α 90° β 111.237(10)° γ 90°
PB  - The Cambridge Crystallographic Data Centre (CCDC)
T1  - CCDC 2110388: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"
DO  - 10.5517/ccdc.csd.cc28v117
ER  - 
@misc{
author = "Pevec, Andrej",
year = "2022",
abstract = "NAMYUZ : diazido-[2-oxy-N,N,N-trimethyl-2-{[1-(1,3-thiazol-2-yl)ethylidene]hydrazinylidene}ethan-1-aminium]-zinc Space Group: P 21/c (14), Cell: a 13.0826(10)Å b 10.2506(7)Å c 13.1685(13)Å, α 90° β 111.237(10)° γ 90°",
publisher = "The Cambridge Crystallographic Data Centre (CCDC)",
title = "CCDC 2110388: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"",
doi = "10.5517/ccdc.csd.cc28v117"
}
Pevec, A.. (2022). CCDC 2110388: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". 
The Cambridge Crystallographic Data Centre (CCDC)..
https://doi.org/10.5517/ccdc.csd.cc28v117
Pevec A. CCDC 2110388: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". 2022;.
doi:10.5517/ccdc.csd.cc28v117 .
Pevec, Andrej, "CCDC 2110388: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"" (2022),
https://doi.org/10.5517/ccdc.csd.cc28v117 . .

CCDC 2110387: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"

Pevec, Andrej

(The Cambridge Crystallographic Data Centre (CCDC), 2022)

TY  - DATA
AU  - Pevec, Andrej
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4860
AB  - NAMYOT : bis(μ-azido)-diazido-bis[2-oxy-N,N,N-trimethyl-2-{[1-(1,3-thiazol-2-yl)ethylidene]hydrazinylidene}ethan-1-aminium]-di-manganese methanol solvate Space Group: P 1 (2), Cell: a 9.6427(5)Å b 10.8396(5)Å c 10.8617(8)Å, α 106.971(5)° β 103.497(5)° γ 112.469(5)°
PB  - The Cambridge Crystallographic Data Centre (CCDC)
T1  - CCDC 2110387: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"
DO  - 10.5517/ccdc.csd.cc28v106
ER  - 
@misc{
author = "Pevec, Andrej",
year = "2022",
abstract = "NAMYOT : bis(μ-azido)-diazido-bis[2-oxy-N,N,N-trimethyl-2-{[1-(1,3-thiazol-2-yl)ethylidene]hydrazinylidene}ethan-1-aminium]-di-manganese methanol solvate Space Group: P 1 (2), Cell: a 9.6427(5)Å b 10.8396(5)Å c 10.8617(8)Å, α 106.971(5)° β 103.497(5)° γ 112.469(5)°",
publisher = "The Cambridge Crystallographic Data Centre (CCDC)",
title = "CCDC 2110387: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"",
doi = "10.5517/ccdc.csd.cc28v106"
}
Pevec, A.. (2022). CCDC 2110387: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". 
The Cambridge Crystallographic Data Centre (CCDC)..
https://doi.org/10.5517/ccdc.csd.cc28v106
Pevec A. CCDC 2110387: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". 2022;.
doi:10.5517/ccdc.csd.cc28v106 .
Pevec, Andrej, "CCDC 2110387: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"" (2022),
https://doi.org/10.5517/ccdc.csd.cc28v106 . .

CCDC 2110386: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"

Pevec, Andrej

(The Cambridge Crystallographic Data Centre (CCDC), 2022)

TY  - DATA
AU  - Pevec, Andrej
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4859
AB  - NAMYIN : azido-[2-oxy-N,N,N-trimethyl-2-{[1-(1,3-thiazol-2-yl)ethylidene]hydrazinylidene}ethan-1-aminium]-(methanol)-copper tetrafluoroborate Space Group: P 21/n (14), Cell: a 7.0033(3)Å b 10.8941(3)Å c 25.6059(9)Å, α 90° β 97.242(4)° γ 90°
PB  - The Cambridge Crystallographic Data Centre (CCDC)
T1  - CCDC 2110386: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"
DO  - 10.5517/ccdc.csd.cc28v0z4
ER  - 
@misc{
author = "Pevec, Andrej",
year = "2022",
abstract = "NAMYIN : azido-[2-oxy-N,N,N-trimethyl-2-{[1-(1,3-thiazol-2-yl)ethylidene]hydrazinylidene}ethan-1-aminium]-(methanol)-copper tetrafluoroborate Space Group: P 21/n (14), Cell: a 7.0033(3)Å b 10.8941(3)Å c 25.6059(9)Å, α 90° β 97.242(4)° γ 90°",
publisher = "The Cambridge Crystallographic Data Centre (CCDC)",
title = "CCDC 2110386: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"",
doi = "10.5517/ccdc.csd.cc28v0z4"
}
Pevec, A.. (2022). CCDC 2110386: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". 
The Cambridge Crystallographic Data Centre (CCDC)..
https://doi.org/10.5517/ccdc.csd.cc28v0z4
Pevec A. CCDC 2110386: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". 2022;.
doi:10.5517/ccdc.csd.cc28v0z4 .
Pevec, Andrej, "CCDC 2110386: Experimental Crystal Structure Determination. Crystallographic data for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"" (2022),
https://doi.org/10.5517/ccdc.csd.cc28v0z4 . .

Supporting information for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"

Stevanović, Nevena; Zlatar, Matija; Novaković, Irena; Pevec, Andrej; Radanović, Dušanka; Matić, Ivana Z.; Đorđić Crnogorac, Marija; Stanojković, Tatjana; Vujčić, Miroslava; Gruden, Maja; Sladić, Dušan; Anđelković, Katarina; Turel, Iztok; Čobeljić, Božidar

(Royal Society of Chemistry (RSC), 2022)

TY  - DATA
AU  - Stevanović, Nevena
AU  - Zlatar, Matija
AU  - Novaković, Irena
AU  - Pevec, Andrej
AU  - Radanović, Dušanka
AU  - Matić, Ivana Z.
AU  - Đorđić Crnogorac, Marija
AU  - Stanojković, Tatjana
AU  - Vujčić, Miroslava
AU  - Gruden, Maja
AU  - Sladić, Dušan
AU  - Anđelković, Katarina
AU  - Turel, Iztok
AU  - Čobeljić, Božidar
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4858
AB  - In this paper, Cu(II), Mn(II) and Zn(II) complexes with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride (HL1Cl) were synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy, elemental analysis and DFT calculations. In all three complexes, a ligand (L1) is coordinated in a deprotonated formally neutral zwitterionic form via NNO donor set atoms. Cu(II) and Zn(II) form mononuclear penta-coordinated complexes [CuL1(N3)(CH3OH)]BF4 and [ZnL1(N3)2], respectively, while Mn(II) forms a binuclear [Mn2L12(μ-1,1-N3)2(N3)2]·2CH3OH complex, with unusual distorted trigonal-prismatic geometry around the metal centers. The antimicrobial activity of these complexes was tested against a panel of Gram-negative and Gram-positive bacteria, two yeasts and one fungal strain. The binuclear Mn(II) complex showed antifungal activity of similar intensity to amphotericin B. Based on the results of the brine shrimp test and DPPH radical scavenging activity, the most active Cu(II) and Mn(II) complexes were selected for evaluation of cytotoxic activity against five malignant cancer cell lines (HeLa, A375, MCF7, PC-3 and A549) and one normal cell line HaCaT. Both complexes showed significant activity. It should be pointed out that the activity of the Mn(II) complex against the MCF7 breast cancer cell line is only slightly weaker than that of cisplatin, but with selectivity to the tumor cell line in comparison to normal HaCaT cells, which is non-existent in the case of cisplatin.
PB  - Royal Society of Chemistry (RSC)
T2  - Dalton Transactions
T1  - Supporting information for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4858
ER  - 
@misc{
author = "Stevanović, Nevena and Zlatar, Matija and Novaković, Irena and Pevec, Andrej and Radanović, Dušanka and Matić, Ivana Z. and Đorđić Crnogorac, Marija and Stanojković, Tatjana and Vujčić, Miroslava and Gruden, Maja and Sladić, Dušan and Anđelković, Katarina and Turel, Iztok and Čobeljić, Božidar",
year = "2022",
abstract = "In this paper, Cu(II), Mn(II) and Zn(II) complexes with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride (HL1Cl) were synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy, elemental analysis and DFT calculations. In all three complexes, a ligand (L1) is coordinated in a deprotonated formally neutral zwitterionic form via NNO donor set atoms. Cu(II) and Zn(II) form mononuclear penta-coordinated complexes [CuL1(N3)(CH3OH)]BF4 and [ZnL1(N3)2], respectively, while Mn(II) forms a binuclear [Mn2L12(μ-1,1-N3)2(N3)2]·2CH3OH complex, with unusual distorted trigonal-prismatic geometry around the metal centers. The antimicrobial activity of these complexes was tested against a panel of Gram-negative and Gram-positive bacteria, two yeasts and one fungal strain. The binuclear Mn(II) complex showed antifungal activity of similar intensity to amphotericin B. Based on the results of the brine shrimp test and DPPH radical scavenging activity, the most active Cu(II) and Mn(II) complexes were selected for evaluation of cytotoxic activity against five malignant cancer cell lines (HeLa, A375, MCF7, PC-3 and A549) and one normal cell line HaCaT. Both complexes showed significant activity. It should be pointed out that the activity of the Mn(II) complex against the MCF7 breast cancer cell line is only slightly weaker than that of cisplatin, but with selectivity to the tumor cell line in comparison to normal HaCaT cells, which is non-existent in the case of cisplatin.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Dalton Transactions",
title = "Supporting information for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4858"
}
Stevanović, N., Zlatar, M., Novaković, I., Pevec, A., Radanović, D., Matić, I. Z., Đorđić Crnogorac, M., Stanojković, T., Vujčić, M., Gruden, M., Sladić, D., Anđelković, K., Turel, I.,& Čobeljić, B.. (2022). Supporting information for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". in Dalton Transactions
Royal Society of Chemistry (RSC)..
https://hdl.handle.net/21.15107/rcub_cherry_4858
Stevanović N, Zlatar M, Novaković I, Pevec A, Radanović D, Matić IZ, Đorđić Crnogorac M, Stanojković T, Vujčić M, Gruden M, Sladić D, Anđelković K, Turel I, Čobeljić B. Supporting information for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity". in Dalton Transactions. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_4858 .
Stevanović, Nevena, Zlatar, Matija, Novaković, Irena, Pevec, Andrej, Radanović, Dušanka, Matić, Ivana Z., Đorđić Crnogorac, Marija, Stanojković, Tatjana, Vujčić, Miroslava, Gruden, Maja, Sladić, Dušan, Anđelković, Katarina, Turel, Iztok, Čobeljić, Božidar, "Supporting information for: "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity"" in Dalton Transactions (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4858 .

Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity

Stevanović, Nevena; Zlatar, Matija; Novaković, Irena; Pevec, Andrej; Radanović, Dušanka; Matić, Ivana Z.; Đorđić Crnogorac, Marija; Stanojković, Tatjana; Vujčić, Miroslava; Gruden, Maja; Sladić, Dušan; Anđelković, Katarina; Turel, Iztok; Čobeljić, Božidar

(Royal Society of Chemistry (RSC), 2022)

TY  - JOUR
AU  - Stevanović, Nevena
AU  - Zlatar, Matija
AU  - Novaković, Irena
AU  - Pevec, Andrej
AU  - Radanović, Dušanka
AU  - Matić, Ivana Z.
AU  - Đorđić Crnogorac, Marija
AU  - Stanojković, Tatjana
AU  - Vujčić, Miroslava
AU  - Gruden, Maja
AU  - Sladić, Dušan
AU  - Anđelković, Katarina
AU  - Turel, Iztok
AU  - Čobeljić, Božidar
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4857
AB  - In this paper, Cu(II), Mn(II) and Zn(II) complexes with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride (HL1Cl) were synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy, elemental analysis and DFT calculations. In all three complexes, a ligand (L1) is coordinated in a deprotonated formally neutral zwitterionic form via NNO donor set atoms. Cu(II) and Zn(II) form mononuclear penta-coordinated complexes [CuL1(N3)(CH3OH)]BF4 and [ZnL1(N3)2], respectively, while Mn(II) forms a binuclear [Mn2L12(μ-1,1-N3)2(N3)2]·2CH3OH complex, with unusual distorted trigonal-prismatic geometry around the metal centers. The antimicrobial activity of these complexes was tested against a panel of Gram-negative and Gram-positive bacteria, two yeasts and one fungal strain. The binuclear Mn(II) complex showed antifungal activity of similar intensity to amphotericin B. Based on the results of the brine shrimp test and DPPH radical scavenging activity, the most active Cu(II) and Mn(II) complexes were selected for evaluation of cytotoxic activity against five malignant cancer cell lines (HeLa, A375, MCF7, PC-3 and A549) and one normal cell line HaCaT. Both complexes showed significant activity. It should be pointed out that the activity of the Mn(II) complex against the MCF7 breast cancer cell line is only slightly weaker than that of cisplatin, but with selectivity to the tumor cell line in comparison to normal HaCaT cells, which is non-existent in the case of cisplatin.
PB  - Royal Society of Chemistry (RSC)
T2  - Dalton Transactions
T1  - Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity
VL  - 51
IS  - 1
SP  - 185
EP  - 196
DO  - 10.1039/D1DT03169D
ER  - 
@article{
author = "Stevanović, Nevena and Zlatar, Matija and Novaković, Irena and Pevec, Andrej and Radanović, Dušanka and Matić, Ivana Z. and Đorđić Crnogorac, Marija and Stanojković, Tatjana and Vujčić, Miroslava and Gruden, Maja and Sladić, Dušan and Anđelković, Katarina and Turel, Iztok and Čobeljić, Božidar",
year = "2022",
abstract = "In this paper, Cu(II), Mn(II) and Zn(II) complexes with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride (HL1Cl) were synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy, elemental analysis and DFT calculations. In all three complexes, a ligand (L1) is coordinated in a deprotonated formally neutral zwitterionic form via NNO donor set atoms. Cu(II) and Zn(II) form mononuclear penta-coordinated complexes [CuL1(N3)(CH3OH)]BF4 and [ZnL1(N3)2], respectively, while Mn(II) forms a binuclear [Mn2L12(μ-1,1-N3)2(N3)2]·2CH3OH complex, with unusual distorted trigonal-prismatic geometry around the metal centers. The antimicrobial activity of these complexes was tested against a panel of Gram-negative and Gram-positive bacteria, two yeasts and one fungal strain. The binuclear Mn(II) complex showed antifungal activity of similar intensity to amphotericin B. Based on the results of the brine shrimp test and DPPH radical scavenging activity, the most active Cu(II) and Mn(II) complexes were selected for evaluation of cytotoxic activity against five malignant cancer cell lines (HeLa, A375, MCF7, PC-3 and A549) and one normal cell line HaCaT. Both complexes showed significant activity. It should be pointed out that the activity of the Mn(II) complex against the MCF7 breast cancer cell line is only slightly weaker than that of cisplatin, but with selectivity to the tumor cell line in comparison to normal HaCaT cells, which is non-existent in the case of cisplatin.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Dalton Transactions",
title = "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity",
volume = "51",
number = "1",
pages = "185-196",
doi = "10.1039/D1DT03169D"
}
Stevanović, N., Zlatar, M., Novaković, I., Pevec, A., Radanović, D., Matić, I. Z., Đorđić Crnogorac, M., Stanojković, T., Vujčić, M., Gruden, M., Sladić, D., Anđelković, K., Turel, I.,& Čobeljić, B.. (2022). Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity. in Dalton Transactions
Royal Society of Chemistry (RSC)., 51(1), 185-196.
https://doi.org/10.1039/D1DT03169D
Stevanović N, Zlatar M, Novaković I, Pevec A, Radanović D, Matić IZ, Đorđić Crnogorac M, Stanojković T, Vujčić M, Gruden M, Sladić D, Anđelković K, Turel I, Čobeljić B. Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity. in Dalton Transactions. 2022;51(1):185-196.
doi:10.1039/D1DT03169D .
Stevanović, Nevena, Zlatar, Matija, Novaković, Irena, Pevec, Andrej, Radanović, Dušanka, Matić, Ivana Z., Đorđić Crnogorac, Marija, Stanojković, Tatjana, Vujčić, Miroslava, Gruden, Maja, Sladić, Dušan, Anđelković, Katarina, Turel, Iztok, Čobeljić, Božidar, "Cu(II), Mn(II) and Zn(II) complexes of hydrazones with a quaternary ammonium moiety: synthesis, experimental and theoretical characterization and cytotoxic activity" in Dalton Transactions, 51, no. 1 (2022):185-196,
https://doi.org/10.1039/D1DT03169D . .
4

Supplementary data for the article: Stevanović, J. Z.; Rakitin, A. R.; Kojić, I. D.; Vuković, N. S.; Stojanović, K. Significance of Infrared Spectroscopic Branching Factor for Investigation of Structural Characteristics of Alkanes, Geochemical Properties and Viscosity of Oils: Scientific Paper. Journal of the Serbian Chemical Society 2022, 87 (1), 41–55. https://doi.org/10.2298/JSC210830091S.

Stevanović, Jelena Z.; Rakitin, Anton R.; Kojić, Ivan D.; Vuković, Nikola S.; Stojanović, Ksenija A.

(Belgrade : Serbian Chemical Society, 2022)

TY  - DATA
AU  - Stevanović, Jelena Z.
AU  - Rakitin, Anton R.
AU  - Kojić, Ivan D.
AU  - Vuković, Nikola S.
AU  - Stojanović, Ksenija A.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4855
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Supplementary data for the article: Stevanović, J. Z.; Rakitin, A. R.; Kojić, I. D.; Vuković, N. S.; Stojanović, K. Significance of Infrared Spectroscopic Branching Factor for Investigation of Structural Characteristics of Alkanes, Geochemical Properties and Viscosity of Oils: Scientific Paper. Journal of the Serbian Chemical Society 2022, 87 (1), 41–55. https://doi.org/10.2298/JSC210830091S.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4855
ER  - 
@misc{
author = "Stevanović, Jelena Z. and Rakitin, Anton R. and Kojić, Ivan D. and Vuković, Nikola S. and Stojanović, Ksenija A.",
year = "2022",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Supplementary data for the article: Stevanović, J. Z.; Rakitin, A. R.; Kojić, I. D.; Vuković, N. S.; Stojanović, K. Significance of Infrared Spectroscopic Branching Factor for Investigation of Structural Characteristics of Alkanes, Geochemical Properties and Viscosity of Oils: Scientific Paper. Journal of the Serbian Chemical Society 2022, 87 (1), 41–55. https://doi.org/10.2298/JSC210830091S.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4855"
}
Stevanović, J. Z., Rakitin, A. R., Kojić, I. D., Vuković, N. S.,& Stojanović, K. A.. (2022). Supplementary data for the article: Stevanović, J. Z.; Rakitin, A. R.; Kojić, I. D.; Vuković, N. S.; Stojanović, K. Significance of Infrared Spectroscopic Branching Factor for Investigation of Structural Characteristics of Alkanes, Geochemical Properties and Viscosity of Oils: Scientific Paper. Journal of the Serbian Chemical Society 2022, 87 (1), 41–55. https://doi.org/10.2298/JSC210830091S.. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society..
https://hdl.handle.net/21.15107/rcub_cherry_4855
Stevanović JZ, Rakitin AR, Kojić ID, Vuković NS, Stojanović KA. Supplementary data for the article: Stevanović, J. Z.; Rakitin, A. R.; Kojić, I. D.; Vuković, N. S.; Stojanović, K. Significance of Infrared Spectroscopic Branching Factor for Investigation of Structural Characteristics of Alkanes, Geochemical Properties and Viscosity of Oils: Scientific Paper. Journal of the Serbian Chemical Society 2022, 87 (1), 41–55. https://doi.org/10.2298/JSC210830091S.. in Journal of the Serbian Chemical Society. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_4855 .
Stevanović, Jelena Z., Rakitin, Anton R., Kojić, Ivan D., Vuković, Nikola S., Stojanović, Ksenija A., "Supplementary data for the article: Stevanović, J. Z.; Rakitin, A. R.; Kojić, I. D.; Vuković, N. S.; Stojanović, K. Significance of Infrared Spectroscopic Branching Factor for Investigation of Structural Characteristics of Alkanes, Geochemical Properties and Viscosity of Oils: Scientific Paper. Journal of the Serbian Chemical Society 2022, 87 (1), 41–55. https://doi.org/10.2298/JSC210830091S." in Journal of the Serbian Chemical Society (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4855 .

Significance of infrared spectroscopic branching factor for investigation of structural characteristics of alkanes, geochemical properties and viscosity of oils: Scientific paper

Stevanović, Jelena Z.; Rakitin, Anton R.; Kojić, Ivan D.; Vuković, Nikola S.; Stojanović, Ksenija A.

(Belgrade : Serbian Chemical Society, 2022)

TY  - JOUR
AU  - Stevanović, Jelena Z.
AU  - Rakitin, Anton R.
AU  - Kojić, Ivan D.
AU  - Vuković, Nikola S.
AU  - Stojanović, Ksenija A.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4854
AB  - A detailed investigation of significance of the infrared (IR) spectro­scopic branching factor (СН2/СН3; the ratio of methylene and methyl group peak heights at 2917–2921 and 2951–2954 cm-1, respectively in the IR spectra) for charac­te­ri­za­tion of alkane structure, geochemical properties and viscosity of 76 oil samples was performed. These oils, originating from 13 Serbian oil fields in SE Pannonian Basin, differ according to source and depositional envi­ronment of organic matter (OM), as well as by thermal maturity and biodeg­rad­ation stage. Methylene and methyl asymmetric stretching peak absorbances were used for the branching factor calculation. CH2 peak positions exhibited3–4 cm-1 red shift with increasing the CH2/CH3 ratio, due to a greater contri­bution of trans vs. gauche rotamers in ali­pha­tic chains. Comparing IR spectra of the oils and model n-alkanes, it was established that the average (CH2)n methylene chain length per СН3 group varied from n = 3.5 to 6.5. The CH2/CH3 ratio showed significant concordance with geochemical para­meters, enabling clear distinction of the oils according to source and deposi­ti­onal environment of OM. At the same time, dependence of the CH2/CH3 ratio on oil maturity in the range from immature to mature was not observed, allowing for an accurate determination of oil genetic types irrespective of maturity. The CH2/CH3 ratio showed good accordance with oil biodegradation scale and oil viscosity.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Significance of infrared spectroscopic branching factor for investigation of structural characteristics of alkanes, geochemical properties and viscosity of oils: Scientific paper
VL  - 87
IS  - 1
SP  - 41
EP  - 55
DO  - 10.2298/JSC210830091S
ER  - 
@article{
author = "Stevanović, Jelena Z. and Rakitin, Anton R. and Kojić, Ivan D. and Vuković, Nikola S. and Stojanović, Ksenija A.",
year = "2022",
abstract = "A detailed investigation of significance of the infrared (IR) spectro­scopic branching factor (СН2/СН3; the ratio of methylene and methyl group peak heights at 2917–2921 and 2951–2954 cm-1, respectively in the IR spectra) for charac­te­ri­za­tion of alkane structure, geochemical properties and viscosity of 76 oil samples was performed. These oils, originating from 13 Serbian oil fields in SE Pannonian Basin, differ according to source and depositional envi­ronment of organic matter (OM), as well as by thermal maturity and biodeg­rad­ation stage. Methylene and methyl asymmetric stretching peak absorbances were used for the branching factor calculation. CH2 peak positions exhibited3–4 cm-1 red shift with increasing the CH2/CH3 ratio, due to a greater contri­bution of trans vs. gauche rotamers in ali­pha­tic chains. Comparing IR spectra of the oils and model n-alkanes, it was established that the average (CH2)n methylene chain length per СН3 group varied from n = 3.5 to 6.5. The CH2/CH3 ratio showed significant concordance with geochemical para­meters, enabling clear distinction of the oils according to source and deposi­ti­onal environment of OM. At the same time, dependence of the CH2/CH3 ratio on oil maturity in the range from immature to mature was not observed, allowing for an accurate determination of oil genetic types irrespective of maturity. The CH2/CH3 ratio showed good accordance with oil biodegradation scale and oil viscosity.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Significance of infrared spectroscopic branching factor for investigation of structural characteristics of alkanes, geochemical properties and viscosity of oils: Scientific paper",
volume = "87",
number = "1",
pages = "41-55",
doi = "10.2298/JSC210830091S"
}
Stevanović, J. Z., Rakitin, A. R., Kojić, I. D., Vuković, N. S.,& Stojanović, K. A.. (2022). Significance of infrared spectroscopic branching factor for investigation of structural characteristics of alkanes, geochemical properties and viscosity of oils: Scientific paper. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 87(1), 41-55.
https://doi.org/10.2298/JSC210830091S
Stevanović JZ, Rakitin AR, Kojić ID, Vuković NS, Stojanović KA. Significance of infrared spectroscopic branching factor for investigation of structural characteristics of alkanes, geochemical properties and viscosity of oils: Scientific paper. in Journal of the Serbian Chemical Society. 2022;87(1):41-55.
doi:10.2298/JSC210830091S .
Stevanović, Jelena Z., Rakitin, Anton R., Kojić, Ivan D., Vuković, Nikola S., Stojanović, Ksenija A., "Significance of infrared spectroscopic branching factor for investigation of structural characteristics of alkanes, geochemical properties and viscosity of oils: Scientific paper" in Journal of the Serbian Chemical Society, 87, no. 1 (2022):41-55,
https://doi.org/10.2298/JSC210830091S . .

A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)

Gajica, Gordana; Šajnović, Aleksandra; Stojanović, Ksenija A.; Schwarzbauer, Jan; Kostić, Aleksandar; Jovančićević, Branimir

(Elsevier, 2022)

TY  - JOUR
AU  - Gajica, Gordana
AU  - Šajnović, Aleksandra
AU  - Stojanović, Ksenija A.
AU  - Schwarzbauer, Jan
AU  - Kostić, Aleksandar
AU  - Jovančićević, Branimir
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4846
AB  - The molecular and isotopic composition of biomarkers in initial bitumen isolated from immature (0.41% Rr) oil shale samples (Aleksinac deposit) and liquid products obtained by pyrolysis in open (OS) and closed (CS) systems are studied. The influence of pyrolysis type and variations of kerogen type on biomarkers composition and their isotopic signatures in liquid products is determined. The applicability of pyrolysis type, numerous biomarkers and carbon isotopic compositions (δ13C) of n-alkanes in liquid pyrolysates is established. Pyrolysis experiments were performed on two selected samples that showed high content of total organic carbon and hydrocarbon generation potential, but also certain variations in sources/depositional environment of organic matter (type I and mixed type I/II kerogen) within previous research of the oil shales sample set. The biomarker signatures were evaluated using gas chromatography-mass spectrometry (GC-MS) and δ13C of individual n-alkanes in bitumen and liquid pyrolysates. The molecular composition of liquid pyrolysates from the OS is very similar to those in initial bitumen, independently on kerogen type, confirming algal origin of organic matter (OM) deposited in lacustrine environment, even more apparently than results of initial bitumen. Therefore, OS can be useful for assessment of source and depositional environment of OM. Pyrolysis in the CS caused more intense thermal alterations, therefore the source fingerprints sometimes notably disappear. The liquid pyrolysates from the CS have the distributions of biomarkers similar to those in crude oils. The biomarker maturity parameters showed slightly higher values in the CS pyrolysate of mixed type I/II kerogen in relation to type I kerogen. δ13C of n-alkanes in liquid pyrolysates from the OS are isotopically lighter in comparison to bitumen, independently on kerogen type. Oppositely, in liquid pyrolysates from the CS, they become heavier than in bitumen, indicating the thermal influence on δ13C signatures, with more pronounced difference for type I kerogen.
PB  - Elsevier
T2  - Marine and Petroleum Geology
T1  - A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)
VL  - 136
SP  - 105383
DO  - 10.1016/j.marpetgeo.2021.105383
ER  - 
@article{
author = "Gajica, Gordana and Šajnović, Aleksandra and Stojanović, Ksenija A. and Schwarzbauer, Jan and Kostić, Aleksandar and Jovančićević, Branimir",
year = "2022",
abstract = "The molecular and isotopic composition of biomarkers in initial bitumen isolated from immature (0.41% Rr) oil shale samples (Aleksinac deposit) and liquid products obtained by pyrolysis in open (OS) and closed (CS) systems are studied. The influence of pyrolysis type and variations of kerogen type on biomarkers composition and their isotopic signatures in liquid products is determined. The applicability of pyrolysis type, numerous biomarkers and carbon isotopic compositions (δ13C) of n-alkanes in liquid pyrolysates is established. Pyrolysis experiments were performed on two selected samples that showed high content of total organic carbon and hydrocarbon generation potential, but also certain variations in sources/depositional environment of organic matter (type I and mixed type I/II kerogen) within previous research of the oil shales sample set. The biomarker signatures were evaluated using gas chromatography-mass spectrometry (GC-MS) and δ13C of individual n-alkanes in bitumen and liquid pyrolysates. The molecular composition of liquid pyrolysates from the OS is very similar to those in initial bitumen, independently on kerogen type, confirming algal origin of organic matter (OM) deposited in lacustrine environment, even more apparently than results of initial bitumen. Therefore, OS can be useful for assessment of source and depositional environment of OM. Pyrolysis in the CS caused more intense thermal alterations, therefore the source fingerprints sometimes notably disappear. The liquid pyrolysates from the CS have the distributions of biomarkers similar to those in crude oils. The biomarker maturity parameters showed slightly higher values in the CS pyrolysate of mixed type I/II kerogen in relation to type I kerogen. δ13C of n-alkanes in liquid pyrolysates from the OS are isotopically lighter in comparison to bitumen, independently on kerogen type. Oppositely, in liquid pyrolysates from the CS, they become heavier than in bitumen, indicating the thermal influence on δ13C signatures, with more pronounced difference for type I kerogen.",
publisher = "Elsevier",
journal = "Marine and Petroleum Geology",
title = "A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)",
volume = "136",
pages = "105383",
doi = "10.1016/j.marpetgeo.2021.105383"
}
Gajica, G., Šajnović, A., Stojanović, K. A., Schwarzbauer, J., Kostić, A.,& Jovančićević, B.. (2022). A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems). in Marine and Petroleum Geology
Elsevier., 136, 105383.
https://doi.org/10.1016/j.marpetgeo.2021.105383
Gajica G, Šajnović A, Stojanović KA, Schwarzbauer J, Kostić A, Jovančićević B. A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems). in Marine and Petroleum Geology. 2022;136:105383.
doi:10.1016/j.marpetgeo.2021.105383 .
Gajica, Gordana, Šajnović, Aleksandra, Stojanović, Ksenija A., Schwarzbauer, Jan, Kostić, Aleksandar, Jovančićević, Branimir, "A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)" in Marine and Petroleum Geology, 136 (2022):105383,
https://doi.org/10.1016/j.marpetgeo.2021.105383 . .

A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)

Gajica, Gordana; Šajnović, Aleksandra; Stojanović, Ksenija A.; Schwarzbauer, Jan; Kostić, Aleksandar; Jovančićević, Branimir

(Elsevier, 2022)

TY  - JOUR
AU  - Gajica, Gordana
AU  - Šajnović, Aleksandra
AU  - Stojanović, Ksenija A.
AU  - Schwarzbauer, Jan
AU  - Kostić, Aleksandar
AU  - Jovančićević, Branimir
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4846
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4847
AB  - The molecular and isotopic composition of biomarkers in initial bitumen isolated from immature (0.41% Rr) oil shale samples (Aleksinac deposit) and liquid products obtained by pyrolysis in open (OS) and closed (CS) systems are studied. The influence of pyrolysis type and variations of kerogen type on biomarkers composition and their isotopic signatures in liquid products is determined. The applicability of pyrolysis type, numerous biomarkers and carbon isotopic compositions (δ13C) of n-alkanes in liquid pyrolysates is established. Pyrolysis experiments were performed on two selected samples that showed high content of total organic carbon and hydrocarbon generation potential, but also certain variations in sources/depositional environment of organic matter (type I and mixed type I/II kerogen) within previous research of the oil shales sample set. The biomarker signatures were evaluated using gas chromatography-mass spectrometry (GC-MS) and δ13C of individual n-alkanes in bitumen and liquid pyrolysates. The molecular composition of liquid pyrolysates from the OS is very similar to those in initial bitumen, independently on kerogen type, confirming algal origin of organic matter (OM) deposited in lacustrine environment, even more apparently than results of initial bitumen. Therefore, OS can be useful for assessment of source and depositional environment of OM. Pyrolysis in the CS caused more intense thermal alterations, therefore the source fingerprints sometimes notably disappear. The liquid pyrolysates from the CS have the distributions of biomarkers similar to those in crude oils. The biomarker maturity parameters showed slightly higher values in the CS pyrolysate of mixed type I/II kerogen in relation to type I kerogen. δ13C of n-alkanes in liquid pyrolysates from the OS are isotopically lighter in comparison to bitumen, independently on kerogen type. Oppositely, in liquid pyrolysates from the CS, they become heavier than in bitumen, indicating the thermal influence on δ13C signatures, with more pronounced difference for type I kerogen.
PB  - Elsevier
T2  - Marine and Petroleum Geology
T1  - A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)
VL  - 136
SP  - 105383
DO  - 10.1016/j.marpetgeo.2021.105383
ER  - 
@article{
author = "Gajica, Gordana and Šajnović, Aleksandra and Stojanović, Ksenija A. and Schwarzbauer, Jan and Kostić, Aleksandar and Jovančićević, Branimir",
year = "2022",
abstract = "The molecular and isotopic composition of biomarkers in initial bitumen isolated from immature (0.41% Rr) oil shale samples (Aleksinac deposit) and liquid products obtained by pyrolysis in open (OS) and closed (CS) systems are studied. The influence of pyrolysis type and variations of kerogen type on biomarkers composition and their isotopic signatures in liquid products is determined. The applicability of pyrolysis type, numerous biomarkers and carbon isotopic compositions (δ13C) of n-alkanes in liquid pyrolysates is established. Pyrolysis experiments were performed on two selected samples that showed high content of total organic carbon and hydrocarbon generation potential, but also certain variations in sources/depositional environment of organic matter (type I and mixed type I/II kerogen) within previous research of the oil shales sample set. The biomarker signatures were evaluated using gas chromatography-mass spectrometry (GC-MS) and δ13C of individual n-alkanes in bitumen and liquid pyrolysates. The molecular composition of liquid pyrolysates from the OS is very similar to those in initial bitumen, independently on kerogen type, confirming algal origin of organic matter (OM) deposited in lacustrine environment, even more apparently than results of initial bitumen. Therefore, OS can be useful for assessment of source and depositional environment of OM. Pyrolysis in the CS caused more intense thermal alterations, therefore the source fingerprints sometimes notably disappear. The liquid pyrolysates from the CS have the distributions of biomarkers similar to those in crude oils. The biomarker maturity parameters showed slightly higher values in the CS pyrolysate of mixed type I/II kerogen in relation to type I kerogen. δ13C of n-alkanes in liquid pyrolysates from the OS are isotopically lighter in comparison to bitumen, independently on kerogen type. Oppositely, in liquid pyrolysates from the CS, they become heavier than in bitumen, indicating the thermal influence on δ13C signatures, with more pronounced difference for type I kerogen.",
publisher = "Elsevier",
journal = "Marine and Petroleum Geology",
title = "A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)",
volume = "136",
pages = "105383",
doi = "10.1016/j.marpetgeo.2021.105383"
}
Gajica, G., Šajnović, A., Stojanović, K. A., Schwarzbauer, J., Kostić, A.,& Jovančićević, B.. (2022). A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems). in Marine and Petroleum Geology
Elsevier., 136, 105383.
https://doi.org/10.1016/j.marpetgeo.2021.105383
Gajica G, Šajnović A, Stojanović KA, Schwarzbauer J, Kostić A, Jovančićević B. A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems). in Marine and Petroleum Geology. 2022;136:105383.
doi:10.1016/j.marpetgeo.2021.105383 .
Gajica, Gordana, Šajnović, Aleksandra, Stojanović, Ksenija A., Schwarzbauer, Jan, Kostić, Aleksandar, Jovančićević, Branimir, "A comparative study of the molecular and isotopic composition of biomarkers in immature oil shale (Aleksinac deposit, Serbia) and its liquid pyrolysis products (open and closed systems)" in Marine and Petroleum Geology, 136 (2022):105383,
https://doi.org/10.1016/j.marpetgeo.2021.105383 . .

Human health risks and benefits assessment based on OCPs, PCBs, toxic elements and fatty acids in the pelagic fish species from the Adriatic Sea

Milićević, Tijana; Herceg Romanić, Snježana; Popović, Aleksandar R.; Mustać, Bosiljka; Đinović-Stojanović, Jasna; Jovanović, Gordana; Relić, Dubravka

(Elsevier, 2022)

TY  - JOUR
AU  - Milićević, Tijana
AU  - Herceg Romanić, Snježana
AU  - Popović, Aleksandar R.
AU  - Mustać, Bosiljka
AU  - Đinović-Stojanović, Jasna
AU  - Jovanović, Gordana
AU  - Relić, Dubravka
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S0045653521025406
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4664
AB  - Benefits and risks of the fish consumption should be assessed to balance the ingestion of healthy omega-3 fatty acids and adverse chemicals. Persistent organic pollutants-POPs (organochlorine pesticides-OCPs and polychlorinated biphenyls-PCB), macro- and micro-elements and fatty acid contents were determined in six fish species from the Adriatic Sea to assess health risks for consumers (worst-case scenario, diseases development risks and benefit-risk). 16 element, 24 POPs and 14 fatty acid contents were determined in six pelagic species which can be used in human diet. Element concentrations were determined by inductively coupled plasma mass spectrometry (ICP-MS), POPs by high-resolution gas chromatography and fatty acid content by gas–liquid chromatography. Diet based on chub mackerel and round sardinella showed lower daily intake (DI) of POPs and highest DI of essential omega-3 fatty acids than other investigated species. Lower ingestion of toxic elements can be observed by consuming anchovy and round sardinella. Based on POP concentrations, there was not observed non-carcinogenic (HI) nor carcinogenic (CR) risks for consumers. Based on element concentrations, there was low HI (0.1 ≥ HI ≥ 1), while the maximum HIs and outlier values (horse mackerel and anchovy samples) implied the presence of HI (HI > 1). The most significant contributor to total non-carcinogenic and carcinogenic risks was inorganic arsenic (inorganic As). Acceptable CR for consumers was assessed, but maximum CR for consumers of horse mackerel and anchovy (CR ≥ 1 × 10–6) showed adverse effects on human health. There were low HIs for developing cardiovascular, nervous, and reproductive diseases, and maximum HIs were higher than 1. Acceptable (1 × 10−4≥CR ≥ 1 × 10−6) risks were observed for developing cancer of nervous system and reproductive organs. Among investigated fish samples, those with higher ƩBR (benefit-risks) and BR for arsenic (As) than median value have a higher risk than benefits in the human diet.
PB  - Elsevier
T2  - Chemosphere
T1  - Human health risks and benefits assessment based on OCPs, PCBs, toxic elements and fatty acids in the pelagic fish species from the Adriatic Sea
VL  - 287
SP  - 132068
DO  - 10.1016/j.chemosphere.2021.132068
ER  - 
@article{
author = "Milićević, Tijana and Herceg Romanić, Snježana and Popović, Aleksandar R. and Mustać, Bosiljka and Đinović-Stojanović, Jasna and Jovanović, Gordana and Relić, Dubravka",
year = "2022",
abstract = "Benefits and risks of the fish consumption should be assessed to balance the ingestion of healthy omega-3 fatty acids and adverse chemicals. Persistent organic pollutants-POPs (organochlorine pesticides-OCPs and polychlorinated biphenyls-PCB), macro- and micro-elements and fatty acid contents were determined in six fish species from the Adriatic Sea to assess health risks for consumers (worst-case scenario, diseases development risks and benefit-risk). 16 element, 24 POPs and 14 fatty acid contents were determined in six pelagic species which can be used in human diet. Element concentrations were determined by inductively coupled plasma mass spectrometry (ICP-MS), POPs by high-resolution gas chromatography and fatty acid content by gas–liquid chromatography. Diet based on chub mackerel and round sardinella showed lower daily intake (DI) of POPs and highest DI of essential omega-3 fatty acids than other investigated species. Lower ingestion of toxic elements can be observed by consuming anchovy and round sardinella. Based on POP concentrations, there was not observed non-carcinogenic (HI) nor carcinogenic (CR) risks for consumers. Based on element concentrations, there was low HI (0.1 ≥ HI ≥ 1), while the maximum HIs and outlier values (horse mackerel and anchovy samples) implied the presence of HI (HI > 1). The most significant contributor to total non-carcinogenic and carcinogenic risks was inorganic arsenic (inorganic As). Acceptable CR for consumers was assessed, but maximum CR for consumers of horse mackerel and anchovy (CR ≥ 1 × 10–6) showed adverse effects on human health. There were low HIs for developing cardiovascular, nervous, and reproductive diseases, and maximum HIs were higher than 1. Acceptable (1 × 10−4≥CR ≥ 1 × 10−6) risks were observed for developing cancer of nervous system and reproductive organs. Among investigated fish samples, those with higher ƩBR (benefit-risks) and BR for arsenic (As) than median value have a higher risk than benefits in the human diet.",
publisher = "Elsevier",
journal = "Chemosphere",
title = "Human health risks and benefits assessment based on OCPs, PCBs, toxic elements and fatty acids in the pelagic fish species from the Adriatic Sea",
volume = "287",
pages = "132068",
doi = "10.1016/j.chemosphere.2021.132068"
}
Milićević, T., Herceg Romanić, S., Popović, A. R., Mustać, B., Đinović-Stojanović, J., Jovanović, G.,& Relić, D.. (2022). Human health risks and benefits assessment based on OCPs, PCBs, toxic elements and fatty acids in the pelagic fish species from the Adriatic Sea. in Chemosphere
Elsevier., 287, 132068.
https://doi.org/10.1016/j.chemosphere.2021.132068
Milićević T, Herceg Romanić S, Popović AR, Mustać B, Đinović-Stojanović J, Jovanović G, Relić D. Human health risks and benefits assessment based on OCPs, PCBs, toxic elements and fatty acids in the pelagic fish species from the Adriatic Sea. in Chemosphere. 2022;287:132068.
doi:10.1016/j.chemosphere.2021.132068 .
Milićević, Tijana, Herceg Romanić, Snježana, Popović, Aleksandar R., Mustać, Bosiljka, Đinović-Stojanović, Jasna, Jovanović, Gordana, Relić, Dubravka, "Human health risks and benefits assessment based on OCPs, PCBs, toxic elements and fatty acids in the pelagic fish species from the Adriatic Sea" in Chemosphere, 287 (2022):132068,
https://doi.org/10.1016/j.chemosphere.2021.132068 . .
1
1
1

Supplementary data for the article: Milićević, T.; Romanić, S. H.; Popović, A.; Mustać, B.; Đinović-Stojanović, J.; Jovanović, G.; Relić, D. Human Health Risks and Benefits Assessment Based on OCPs, PCBs, Toxic Elements and Fatty Acids in the Pelagic Fish Species from the Adriatic Sea. Chemosphere 2022, 287, 132068. https://doi.org/10.1016/j.chemosphere.2021.132068.

Milićević, Tijana; Herceg Romanić, Snježana; Popović, Aleksandar R.; Mustać, Bosiljka; Đinović-Stojanović, Jasna; Jovanović, Gordana; Relić, Dubravka

(Elsevier, 2022)

TY  - DATA
AU  - Milićević, Tijana
AU  - Herceg Romanić, Snježana
AU  - Popović, Aleksandar R.
AU  - Mustać, Bosiljka
AU  - Đinović-Stojanović, Jasna
AU  - Jovanović, Gordana
AU  - Relić, Dubravka
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S0045653521025406
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4665
PB  - Elsevier
T2  - Chemosphere
T1  - Supplementary data for the article: Milićević, T.; Romanić, S. H.; Popović, A.; Mustać, B.; Đinović-Stojanović, J.; Jovanović, G.; Relić, D. Human Health Risks and Benefits Assessment Based on OCPs, PCBs, Toxic Elements and Fatty Acids in the Pelagic Fish Species from the Adriatic Sea. Chemosphere 2022, 287, 132068. https://doi.org/10.1016/j.chemosphere.2021.132068.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4665
ER  - 
@misc{
author = "Milićević, Tijana and Herceg Romanić, Snježana and Popović, Aleksandar R. and Mustać, Bosiljka and Đinović-Stojanović, Jasna and Jovanović, Gordana and Relić, Dubravka",
year = "2022",
publisher = "Elsevier",
journal = "Chemosphere",
title = "Supplementary data for the article: Milićević, T.; Romanić, S. H.; Popović, A.; Mustać, B.; Đinović-Stojanović, J.; Jovanović, G.; Relić, D. Human Health Risks and Benefits Assessment Based on OCPs, PCBs, Toxic Elements and Fatty Acids in the Pelagic Fish Species from the Adriatic Sea. Chemosphere 2022, 287, 132068. https://doi.org/10.1016/j.chemosphere.2021.132068.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4665"
}
Milićević, T., Herceg Romanić, S., Popović, A. R., Mustać, B., Đinović-Stojanović, J., Jovanović, G.,& Relić, D.. (2022). Supplementary data for the article: Milićević, T.; Romanić, S. H.; Popović, A.; Mustać, B.; Đinović-Stojanović, J.; Jovanović, G.; Relić, D. Human Health Risks and Benefits Assessment Based on OCPs, PCBs, Toxic Elements and Fatty Acids in the Pelagic Fish Species from the Adriatic Sea. Chemosphere 2022, 287, 132068. https://doi.org/10.1016/j.chemosphere.2021.132068.. in Chemosphere
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4665
Milićević T, Herceg Romanić S, Popović AR, Mustać B, Đinović-Stojanović J, Jovanović G, Relić D. Supplementary data for the article: Milićević, T.; Romanić, S. H.; Popović, A.; Mustać, B.; Đinović-Stojanović, J.; Jovanović, G.; Relić, D. Human Health Risks and Benefits Assessment Based on OCPs, PCBs, Toxic Elements and Fatty Acids in the Pelagic Fish Species from the Adriatic Sea. Chemosphere 2022, 287, 132068. https://doi.org/10.1016/j.chemosphere.2021.132068.. in Chemosphere. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_4665 .
Milićević, Tijana, Herceg Romanić, Snježana, Popović, Aleksandar R., Mustać, Bosiljka, Đinović-Stojanović, Jasna, Jovanović, Gordana, Relić, Dubravka, "Supplementary data for the article: Milićević, T.; Romanić, S. H.; Popović, A.; Mustać, B.; Đinović-Stojanović, J.; Jovanović, G.; Relić, D. Human Health Risks and Benefits Assessment Based on OCPs, PCBs, Toxic Elements and Fatty Acids in the Pelagic Fish Species from the Adriatic Sea. Chemosphere 2022, 287, 132068. https://doi.org/10.1016/j.chemosphere.2021.132068." in Chemosphere (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4665 .

Ligand binding to fibrinogen influences its structure and function

Gligorijević, Nikola; Minić, Simeon L.; Radomirović, Mirjana Ž.; Lević, Steva M.; Nikolić, Milan; Ćirković-Veličković, Tanja; Nedić, Olgica

(University of Novi Sad - Faculty of Sciences, Department of Biology, 2021)

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Minić, Simeon L.
AU  - Radomirović, Mirjana Ž.
AU  - Lević, Steva M.
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
AU  - Nedić, Olgica
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4862
AB  - Fibrinogen is a plasma protein that is highly susceptible to oxidation. Because of this chemical modification, fibrinogen acquires thrombogenic characteristics under different pathophysiological conditions. Increased carbonyl content and reduced porosity impair the degradation of formed fibrin mediated by plasmin. Fibrinogen is capable of interacting with many proteins, ions, and small molecules. These interactions can modify the functions of this protein. The discovery of new binding partners that may protect fibrinogen from harmful oxidation and, thus, preserve its normal function is essential. Some of the newly detected interactions between fibrinogen and small, natural bioactive molecules, together with the influence of these interactions on the structure and function of fibrinogen, will be presented in this text.
PB  - University of Novi Sad - Faculty of Sciences, Department of Biology
T2  - Biologia Serbica
T1  - Ligand binding to fibrinogen influences its structure and function
VL  - 43
IS  - 1
DO  - 10.5281/zenodo.5512285
ER  - 
@article{
author = "Gligorijević, Nikola and Minić, Simeon L. and Radomirović, Mirjana Ž. and Lević, Steva M. and Nikolić, Milan and Ćirković-Veličković, Tanja and Nedić, Olgica",
year = "2021",
abstract = "Fibrinogen is a plasma protein that is highly susceptible to oxidation. Because of this chemical modification, fibrinogen acquires thrombogenic characteristics under different pathophysiological conditions. Increased carbonyl content and reduced porosity impair the degradation of formed fibrin mediated by plasmin. Fibrinogen is capable of interacting with many proteins, ions, and small molecules. These interactions can modify the functions of this protein. The discovery of new binding partners that may protect fibrinogen from harmful oxidation and, thus, preserve its normal function is essential. Some of the newly detected interactions between fibrinogen and small, natural bioactive molecules, together with the influence of these interactions on the structure and function of fibrinogen, will be presented in this text.",
publisher = "University of Novi Sad - Faculty of Sciences, Department of Biology",
journal = "Biologia Serbica",
title = "Ligand binding to fibrinogen influences its structure and function",
volume = "43",
number = "1",
doi = "10.5281/zenodo.5512285"
}
Gligorijević, N., Minić, S. L., Radomirović, M. Ž., Lević, S. M., Nikolić, M., Ćirković-Veličković, T.,& Nedić, O.. (2021). Ligand binding to fibrinogen influences its structure and function. in Biologia Serbica
University of Novi Sad - Faculty of Sciences, Department of Biology., 43(1).
https://doi.org/10.5281/zenodo.5512285
Gligorijević N, Minić SL, Radomirović MŽ, Lević SM, Nikolić M, Ćirković-Veličković T, Nedić O. Ligand binding to fibrinogen influences its structure and function. in Biologia Serbica. 2021;43(1).
doi:10.5281/zenodo.5512285 .
Gligorijević, Nikola, Minić, Simeon L., Radomirović, Mirjana Ž., Lević, Steva M., Nikolić, Milan, Ćirković-Veličković, Tanja, Nedić, Olgica, "Ligand binding to fibrinogen influences its structure and function" in Biologia Serbica, 43, no. 1 (2021),
https://doi.org/10.5281/zenodo.5512285 . .

Aggregability and digestibility study of fruit juice fortified camel milk powder proteins

Khulal, Urmila; Ghnimi, Sami; Stevanović, Nikola; Rajković, Andreja; Ćirković-Veličković, Tanja

(Elsevier, 2021)

TY  - JOUR
AU  - Khulal, Urmila
AU  - Ghnimi, Sami
AU  - Stevanović, Nikola
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4746
AB  - In this work, we observed the effect of grape juice (% concentrated juice/% concentrated camel milk: GJ20/80, GJ50/50) and pomegranate juice (PJ20/80, PJ40/60) fortification on camel milk (CM) protein solubility and digestibility. Proteins were dissolved in sodium phosphate buffer to 50 mg/ml and defatted prior Bradford assay of protein concentration, then analyzed by Size Exclusion-Ultra High-Performance Liquid chromatography (SE-UHPLC). The CM protein aggregation and their stability were further monitored at different pH 2.0, 4.0, and 7.5 via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Freeze dried CM (FDCM) was the reference sample and our results showed that GJ50/50 and PJ40/60 with the highest fruit juice ratio had the lowest protein content in the supernatant, hence the decreased solubility. SE-UHPLC of supernatants showed a slight decrease in retention times of 11 kDa and 62 kDa proteins for GJ50/50 and PJ40/60 suggesting a possibility of adduct formation due to fortification leading to higher molecular weight. The simulated static in vitro gastrointestinal digestion of samples revealed that most soluble proteins were readily digested by pepsin, trypsin and chymotrypsin enzymes leading to small peptides. However, the SDS PAGE of pellets showed the partial resistance of casein and α-lactalbumin against peptic digestion.
PB  - Elsevier
T2  - LWT
T1  - Aggregability and digestibility study of fruit juice fortified camel milk powder proteins
VL  - 152
SP  - 112250
DO  - 10.1016/j.lwt.2021.112250
ER  - 
@article{
author = "Khulal, Urmila and Ghnimi, Sami and Stevanović, Nikola and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "In this work, we observed the effect of grape juice (% concentrated juice/% concentrated camel milk: GJ20/80, GJ50/50) and pomegranate juice (PJ20/80, PJ40/60) fortification on camel milk (CM) protein solubility and digestibility. Proteins were dissolved in sodium phosphate buffer to 50 mg/ml and defatted prior Bradford assay of protein concentration, then analyzed by Size Exclusion-Ultra High-Performance Liquid chromatography (SE-UHPLC). The CM protein aggregation and their stability were further monitored at different pH 2.0, 4.0, and 7.5 via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Freeze dried CM (FDCM) was the reference sample and our results showed that GJ50/50 and PJ40/60 with the highest fruit juice ratio had the lowest protein content in the supernatant, hence the decreased solubility. SE-UHPLC of supernatants showed a slight decrease in retention times of 11 kDa and 62 kDa proteins for GJ50/50 and PJ40/60 suggesting a possibility of adduct formation due to fortification leading to higher molecular weight. The simulated static in vitro gastrointestinal digestion of samples revealed that most soluble proteins were readily digested by pepsin, trypsin and chymotrypsin enzymes leading to small peptides. However, the SDS PAGE of pellets showed the partial resistance of casein and α-lactalbumin against peptic digestion.",
publisher = "Elsevier",
journal = "LWT",
title = "Aggregability and digestibility study of fruit juice fortified camel milk powder proteins",
volume = "152",
pages = "112250",
doi = "10.1016/j.lwt.2021.112250"
}
Khulal, U., Ghnimi, S., Stevanović, N., Rajković, A.,& Ćirković-Veličković, T.. (2021). Aggregability and digestibility study of fruit juice fortified camel milk powder proteins. in LWT
Elsevier., 152, 112250.
https://doi.org/10.1016/j.lwt.2021.112250
Khulal U, Ghnimi S, Stevanović N, Rajković A, Ćirković-Veličković T. Aggregability and digestibility study of fruit juice fortified camel milk powder proteins. in LWT. 2021;152:112250.
doi:10.1016/j.lwt.2021.112250 .
Khulal, Urmila, Ghnimi, Sami, Stevanović, Nikola, Rajković, Andreja, Ćirković-Veličković, Tanja, "Aggregability and digestibility study of fruit juice fortified camel milk powder proteins" in LWT, 152 (2021):112250,
https://doi.org/10.1016/j.lwt.2021.112250 . .
5
1

Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.

Khulal, Urmila; Ghnimi, Sami; Stevanović, Nikola; Rajković, Andreja; Ćirković-Veličković, Tanja

(Elsevier, 2021)

TY  - DATA
AU  - Khulal, Urmila
AU  - Ghnimi, Sami
AU  - Stevanović, Nikola
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4747
PB  - Elsevier
T2  - LWT
T1  - Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.
VL  - 152
SP  - 112250
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4747
ER  - 
@misc{
author = "Khulal, Urmila and Ghnimi, Sami and Stevanović, Nikola and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
publisher = "Elsevier",
journal = "LWT",
title = "Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.",
volume = "152",
pages = "112250",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4747"
}
Khulal, U., Ghnimi, S., Stevanović, N., Rajković, A.,& Ćirković-Veličković, T.. (2021). Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.. in LWT
Elsevier., 152, 112250.
https://hdl.handle.net/21.15107/rcub_cherry_4747
Khulal U, Ghnimi S, Stevanović N, Rajković A, Ćirković-Veličković T. Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.. in LWT. 2021;152:112250.
https://hdl.handle.net/21.15107/rcub_cherry_4747 .
Khulal, Urmila, Ghnimi, Sami, Stevanović, Nikola, Rajković, Andreja, Ćirković-Veličković, Tanja, "Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250." in LWT, 152 (2021):112250,
https://hdl.handle.net/21.15107/rcub_cherry_4747 .

Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation

Mutić, Jelena; Jovanović, Vesna; Jacxsens, Liesbeth; Tondeleir, Jannes; Ristivojević, Petar; Đurđić, Slađana; Rajković, Andreja; Ćirković-Veličković, Tanja

(MDPI, 2021)

TY  - JOUR
AU  - Mutić, Jelena
AU  - Jovanović, Vesna
AU  - Jacxsens, Liesbeth
AU  - Tondeleir, Jannes
AU  - Ristivojević, Petar
AU  - Đurđić, Slađana
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4825
AB  - Bivalves are a good source of nutrients but also a potential source of environmental contaminants, which could pose a risk for consumers. The aims of this study were: the determination of 16 elements by ICP-MS in 48 samples of five bivalve species purchased from market in Korea; the identification of elements useful for species classification using multivariate analyses; and the benefit-risk evaluation associated to the consumption of these bivalves. The highest difference among content of elements between species was found for Cd, Mn, Ni, Zn, and Fe. Partial last squares discriminant analysis revealed elements with a VIP score >1 which were considered as the most relevant for explaining certain species. As, Cd, Co, and Ni were found as taxonomical markers of V. philippinarum; Mn, Zn, Mg, and Na of A. irradians; and Cd, Ni, and Fe of M. yessoensis. These species could serve as good dietary sources of essential elements. Cd exposure by consumption of Manila clams is not representing a health risk for the Korean population; however, through consumption of Yesso scallops, 5.3% of the Korean population has a potential health risk. Removal of the digestive gland before eating will drastically reduce the amount of Cd ingested.
PB  - MDPI
T2  - Foods
T1  - Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation
VL  - 10
IS  - 11
SP  - 2690
DO  - 10.3390/foods10112690
ER  - 
@article{
author = "Mutić, Jelena and Jovanović, Vesna and Jacxsens, Liesbeth and Tondeleir, Jannes and Ristivojević, Petar and Đurđić, Slađana and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Bivalves are a good source of nutrients but also a potential source of environmental contaminants, which could pose a risk for consumers. The aims of this study were: the determination of 16 elements by ICP-MS in 48 samples of five bivalve species purchased from market in Korea; the identification of elements useful for species classification using multivariate analyses; and the benefit-risk evaluation associated to the consumption of these bivalves. The highest difference among content of elements between species was found for Cd, Mn, Ni, Zn, and Fe. Partial last squares discriminant analysis revealed elements with a VIP score >1 which were considered as the most relevant for explaining certain species. As, Cd, Co, and Ni were found as taxonomical markers of V. philippinarum; Mn, Zn, Mg, and Na of A. irradians; and Cd, Ni, and Fe of M. yessoensis. These species could serve as good dietary sources of essential elements. Cd exposure by consumption of Manila clams is not representing a health risk for the Korean population; however, through consumption of Yesso scallops, 5.3% of the Korean population has a potential health risk. Removal of the digestive gland before eating will drastically reduce the amount of Cd ingested.",
publisher = "MDPI",
journal = "Foods",
title = "Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation",
volume = "10",
number = "11",
pages = "2690",
doi = "10.3390/foods10112690"
}
Mutić, J., Jovanović, V., Jacxsens, L., Tondeleir, J., Ristivojević, P., Đurđić, S., Rajković, A.,& Ćirković-Veličković, T.. (2021). Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. in Foods
MDPI., 10(11), 2690.
https://doi.org/10.3390/foods10112690
Mutić J, Jovanović V, Jacxsens L, Tondeleir J, Ristivojević P, Đurđić S, Rajković A, Ćirković-Veličković T. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. in Foods. 2021;10(11):2690.
doi:10.3390/foods10112690 .
Mutić, Jelena, Jovanović, Vesna, Jacxsens, Liesbeth, Tondeleir, Jannes, Ristivojević, Petar, Đurđić, Slađana, Rajković, Andreja, Ćirković-Veličković, Tanja, "Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation" in Foods, 10, no. 11 (2021):2690,
https://doi.org/10.3390/foods10112690 . .
1

Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.

Mutić, Jelena; Jovanović, Vesna; Jacxsens, Liesbeth; Tondeleir, Jannes; Ristivojević, Petar; Đurđić, Slađana; Rajković, Andreja; Ćirković-Veličković, Tanja

(MDPI, 2021)

TY  - DATA
AU  - Mutić, Jelena
AU  - Jovanović, Vesna
AU  - Jacxsens, Liesbeth
AU  - Tondeleir, Jannes
AU  - Ristivojević, Petar
AU  - Đurđić, Slađana
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4826
PB  - MDPI
T2  - Foods
T1  - Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4826
ER  - 
@misc{
author = "Mutić, Jelena and Jovanović, Vesna and Jacxsens, Liesbeth and Tondeleir, Jannes and Ristivojević, Petar and Đurđić, Slađana and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
publisher = "MDPI",
journal = "Foods",
title = "Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4826"
}
Mutić, J., Jovanović, V., Jacxsens, L., Tondeleir, J., Ristivojević, P., Đurđić, S., Rajković, A.,& Ćirković-Veličković, T.. (2021). Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.. in Foods
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_4826
Mutić J, Jovanović V, Jacxsens L, Tondeleir J, Ristivojević P, Đurđić S, Rajković A, Ćirković-Veličković T. Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.. in Foods. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4826 .
Mutić, Jelena, Jovanović, Vesna, Jacxsens, Liesbeth, Tondeleir, Jannes, Ristivojević, Petar, Đurđić, Slađana, Rajković, Andreja, Ćirković-Veličković, Tanja, "Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690." in Foods (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4826 .

Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity

Gligorijević, Nikola; Minić, Simeon; Radibratović, Milica; Papadimitriou, Vassiliki; Nedić, Olgica; Sotiroudis, Theodore G.; Nikolić, Milan

(Elsevier, 2021)

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Minić, Simeon
AU  - Radibratović, Milica
AU  - Papadimitriou, Vassiliki
AU  - Nedić, Olgica
AU  - Sotiroudis, Theodore G.
AU  - Nikolić, Milan 
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S1386142521000597
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4486
AB  - Phycocyanobilin is a dark blue linear tetrapyrrole chromophore covalently attached to protein subunits of phycobiliproteins present in the light-harvesting complexes of the cyanobacteria Arthrospira platensis (Spirulina “superfood”). It shows exceptional health-promoting properties and emerging use in various fields of bioscience and industry. This study aims to examine the mutual impact of phycocyanobilin interactions with catalase, a life-essential antioxidant enzyme. Fluorescence quenching experiments demonstrated moderate binding (Ka of 3.9 × 104 M−1 at 25 °C; n = 0.89) (static type), while van't Hoff plot points to an enthalpically driven ligand binding (ΔG = −28.2 kJ mol−1; ΔH = −41.9 kJ mol−1). No significant changes in protein secondary structures (α-helix content ~22%) and thermal protein stability in terms of enzyme tetramer subunits (Tm ~ 64 °C) were detected upon ligand binding. Alterations in the tertiary catalase structure were found without adverse effects on enzyme activity (~2 × 106 IU/mL). The docking study results indicated that the ligand most likely binds to amino acid residues (Asn141, Arg 362, Tyr369 and Asn384) near the cavity between the enzyme homotetramer subunits not related to the active site. Finally, complex formation protects the pigment from free-radical induced oxidation (bleaching), suggesting possible prolongation of its half-life and bioactivity in vivo if bound to catalase.
PB  - Elsevier
T2  - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
T2  - Spectrochimica Acta Part A: Molecular and Biomolecular SpectroscopySpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
T1  - Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity
VL  - 251
SP  - 119483
DO  - 10.1016/j.saa.2021.119483
ER  - 
@article{
author = "Gligorijević, Nikola and Minić, Simeon and Radibratović, Milica and Papadimitriou, Vassiliki and Nedić, Olgica and Sotiroudis, Theodore G. and Nikolić, Milan ",
year = "2021",
abstract = "Phycocyanobilin is a dark blue linear tetrapyrrole chromophore covalently attached to protein subunits of phycobiliproteins present in the light-harvesting complexes of the cyanobacteria Arthrospira platensis (Spirulina “superfood”). It shows exceptional health-promoting properties and emerging use in various fields of bioscience and industry. This study aims to examine the mutual impact of phycocyanobilin interactions with catalase, a life-essential antioxidant enzyme. Fluorescence quenching experiments demonstrated moderate binding (Ka of 3.9 × 104 M−1 at 25 °C; n = 0.89) (static type), while van't Hoff plot points to an enthalpically driven ligand binding (ΔG = −28.2 kJ mol−1; ΔH = −41.9 kJ mol−1). No significant changes in protein secondary structures (α-helix content ~22%) and thermal protein stability in terms of enzyme tetramer subunits (Tm ~ 64 °C) were detected upon ligand binding. Alterations in the tertiary catalase structure were found without adverse effects on enzyme activity (~2 × 106 IU/mL). The docking study results indicated that the ligand most likely binds to amino acid residues (Asn141, Arg 362, Tyr369 and Asn384) near the cavity between the enzyme homotetramer subunits not related to the active site. Finally, complex formation protects the pigment from free-radical induced oxidation (bleaching), suggesting possible prolongation of its half-life and bioactivity in vivo if bound to catalase.",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Spectrochimica Acta Part A: Molecular and Biomolecular SpectroscopySpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy",
title = "Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity",
volume = "251",
pages = "119483",
doi = "10.1016/j.saa.2021.119483"
}
Gligorijević, N., Minić, S., Radibratović, M., Papadimitriou, V., Nedić, O., Sotiroudis, T. G.,& Nikolić, M.. (2021). Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Elsevier., 251, 119483.
https://doi.org/10.1016/j.saa.2021.119483
Gligorijević N, Minić S, Radibratović M, Papadimitriou V, Nedić O, Sotiroudis TG, Nikolić M. Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2021;251:119483.
doi:10.1016/j.saa.2021.119483 .
Gligorijević, Nikola, Minić, Simeon, Radibratović, Milica, Papadimitriou, Vassiliki, Nedić, Olgica, Sotiroudis, Theodore G., Nikolić, Milan , "Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity" in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 251 (2021):119483,
https://doi.org/10.1016/j.saa.2021.119483 . .
1

Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein

Šunderić, Miloš; Vasović, Tamara; Milčić, Miloš K.; Miljević, Čedo; Nedić, Olgica; Nikolić, Milan ; Gligorijević, Nikola

(Elsevier, 2021)

TY  - JOUR
AU  - Šunderić, Miloš
AU  - Vasović, Tamara
AU  - Milčić, Miloš K.
AU  - Miljević, Čedo
AU  - Nedić, Olgica
AU  - Nikolić, Milan 
AU  - Gligorijević, Nikola
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0141813021009284
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4538
AB  - In this study, the interaction between clozapine, an atypical antipsychotic drug, and alpha-2-macroglobulin (α2M), a multipurpose anti-proteinase, was investigated under simulated (patho) physiological conditions using multiple spectroscopic techniques and molecular modeling. It was found that α2M binds clozapine with a moderate affinity (the binding constant of 0.9 × 105 M−1 at 37 °C). The preferable binding site for both clozapine's atropisomers was revealed to be a large pocket at the interface of C and D monomer subunits of the protein. Hydrogen bonds and the hydrophobic effect were proposed as dominant forces in complex formation. The binding of clozapine did not induce significant conformational change of the protein, as confirmed by virtually unaltered α2M secondary structure and anti-proteinase activity. However, both clozapine and α2M shielded each other from the deleterious influence of strong oxidants: sodium hypochlorite and 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH). Moreover, clozapine in a concentration range that is usually targeted in the plasma during patients' treatment effectively protected the anti-proteinase activity of α2M under AAPH-induced free radical overproduction. Our results suggest that the cooperation between α2M and clozapine may be a path by which these two molecules synergistically protect neural tissue against injury caused by disturbed proteostasis or oxidative stress.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein
VL  - 183
SP  - 502
EP  - 512
DO  - 10.1016/j.ijbiomac.2021.04.155
ER  - 
@article{
author = "Šunderić, Miloš and Vasović, Tamara and Milčić, Miloš K. and Miljević, Čedo and Nedić, Olgica and Nikolić, Milan  and Gligorijević, Nikola",
year = "2021",
abstract = "In this study, the interaction between clozapine, an atypical antipsychotic drug, and alpha-2-macroglobulin (α2M), a multipurpose anti-proteinase, was investigated under simulated (patho) physiological conditions using multiple spectroscopic techniques and molecular modeling. It was found that α2M binds clozapine with a moderate affinity (the binding constant of 0.9 × 105 M−1 at 37 °C). The preferable binding site for both clozapine's atropisomers was revealed to be a large pocket at the interface of C and D monomer subunits of the protein. Hydrogen bonds and the hydrophobic effect were proposed as dominant forces in complex formation. The binding of clozapine did not induce significant conformational change of the protein, as confirmed by virtually unaltered α2M secondary structure and anti-proteinase activity. However, both clozapine and α2M shielded each other from the deleterious influence of strong oxidants: sodium hypochlorite and 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH). Moreover, clozapine in a concentration range that is usually targeted in the plasma during patients' treatment effectively protected the anti-proteinase activity of α2M under AAPH-induced free radical overproduction. Our results suggest that the cooperation between α2M and clozapine may be a path by which these two molecules synergistically protect neural tissue against injury caused by disturbed proteostasis or oxidative stress.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein",
volume = "183",
pages = "502-512",
doi = "10.1016/j.ijbiomac.2021.04.155"
}
Šunderić, M., Vasović, T., Milčić, M. K., Miljević, Č., Nedić, O., Nikolić, M.,& Gligorijević, N.. (2021). Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein. in International Journal of Biological Macromolecules
Elsevier., 183, 502-512.
https://doi.org/10.1016/j.ijbiomac.2021.04.155
Šunderić M, Vasović T, Milčić MK, Miljević Č, Nedić O, Nikolić M, Gligorijević N. Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein. in International Journal of Biological Macromolecules. 2021;183:502-512.
doi:10.1016/j.ijbiomac.2021.04.155 .
Šunderić, Miloš, Vasović, Tamara, Milčić, Miloš K., Miljević, Čedo, Nedić, Olgica, Nikolić, Milan , Gligorijević, Nikola, "Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein" in International Journal of Biological Macromolecules, 183 (2021):502-512,
https://doi.org/10.1016/j.ijbiomac.2021.04.155 . .
1

Structural, antioxidant, antiproliferative and in‒silico study of pyridine-based hydrazonyl‒selenazoles and their sulphur isosteres

Araškov, Jovana B.; Nikolić, Milan; Armaković, Stevan; Armaković, Sanja; Rodić, Marko; Višnjevac, Aleksandar; Padrón, José M.; Todorović, Tamara ; Filipović, Nenad R.

(Elsevier, 2021)

TY  - JOUR
AU  - Araškov, Jovana B.
AU  - Nikolić, Milan
AU  - Armaković, Stevan
AU  - Armaković, Sanja
AU  - Rodić, Marko
AU  - Višnjevac, Aleksandar
AU  - Padrón, José M.
AU  - Todorović, Tamara 
AU  - Filipović, Nenad R.
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0022286021006451
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4539
AB  - To evaluate the impact of chalcogen atom type, we performed a comparative study of antioxidant capacity and antiproliferative activity of a focused library of three pyridine-based hydrazonyl-1,3-selenazoles and their sulfur isosteres in five antioxidant assays and in six human solid tumor cell lines, respectively. In-silico calculations were further used to check pharmacokinetic profiles of investigated compounds such as drug-likeness parameters and interaction with water. Generally, selenium compounds appear to be more potent in comparison to sulfur isosteres in the performed essays.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Structural, antioxidant, antiproliferative and in‒silico study of pyridine-based hydrazonyl‒selenazoles and their sulphur isosteres
VL  - 1240
SP  - 130512
DO  - 10.1016/j.molstruc.2021.130512
ER  - 
@article{
author = "Araškov, Jovana B. and Nikolić, Milan and Armaković, Stevan and Armaković, Sanja and Rodić, Marko and Višnjevac, Aleksandar and Padrón, José M. and Todorović, Tamara  and Filipović, Nenad R.",
year = "2021",
abstract = "To evaluate the impact of chalcogen atom type, we performed a comparative study of antioxidant capacity and antiproliferative activity of a focused library of three pyridine-based hydrazonyl-1,3-selenazoles and their sulfur isosteres in five antioxidant assays and in six human solid tumor cell lines, respectively. In-silico calculations were further used to check pharmacokinetic profiles of investigated compounds such as drug-likeness parameters and interaction with water. Generally, selenium compounds appear to be more potent in comparison to sulfur isosteres in the performed essays.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Structural, antioxidant, antiproliferative and in‒silico study of pyridine-based hydrazonyl‒selenazoles and their sulphur isosteres",
volume = "1240",
pages = "130512",
doi = "10.1016/j.molstruc.2021.130512"
}
Araškov, J. B., Nikolić, M., Armaković, S., Armaković, S., Rodić, M., Višnjevac, A., Padrón, J. M., Todorović, T.,& Filipović, N. R.. (2021). Structural, antioxidant, antiproliferative and in‒silico study of pyridine-based hydrazonyl‒selenazoles and their sulphur isosteres. in Journal of Molecular Structure
Elsevier., 1240, 130512.
https://doi.org/10.1016/j.molstruc.2021.130512
Araškov JB, Nikolić M, Armaković S, Armaković S, Rodić M, Višnjevac A, Padrón JM, Todorović T, Filipović NR. Structural, antioxidant, antiproliferative and in‒silico study of pyridine-based hydrazonyl‒selenazoles and their sulphur isosteres. in Journal of Molecular Structure. 2021;1240:130512.
doi:10.1016/j.molstruc.2021.130512 .
Araškov, Jovana B., Nikolić, Milan, Armaković, Stevan, Armaković, Sanja, Rodić, Marko, Višnjevac, Aleksandar, Padrón, José M., Todorović, Tamara , Filipović, Nenad R., "Structural, antioxidant, antiproliferative and in‒silico study of pyridine-based hydrazonyl‒selenazoles and their sulphur isosteres" in Journal of Molecular Structure, 1240 (2021):130512,
https://doi.org/10.1016/j.molstruc.2021.130512 . .
7
2
1

Supplementary data for the article: Araškov, J. B.; Nikolić, M.; Armaković, S.; Armaković, S.; Rodić, M.; Višnjevac, A.; Padrón, J. M.; Todorović, T. R.; Filipović, N. R. Structural, Antioxidant, Antiproliferative and In‒silico Study of Pyridine-Based Hydrazonyl‒selenazoles and Their Sulphur Isosteres. Journal of Molecular Structure 2021, 1240, 130512. https://doi.org/10.1016/j.molstruc.2021.130512.

Araškov, Jovana B.; Nikolić, Milan; Armaković, Stevan; Armaković, Sanja; Rodić, Marko; Višnjevac, Aleksandar; Padrón, José M.; Todorović, Tamara; Filipović, Nenad R.

(Elsevier, 2021)

TY  - DATA
AU  - Araškov, Jovana B.
AU  - Nikolić, Milan
AU  - Armaković, Stevan
AU  - Armaković, Sanja
AU  - Rodić, Marko
AU  - Višnjevac, Aleksandar
AU  - Padrón, José M.
AU  - Todorović, Tamara
AU  - Filipović, Nenad R.
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0022286021006451
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4540
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Supplementary data for the article: Araškov, J. B.; Nikolić, M.; Armaković, S.; Armaković, S.; Rodić, M.; Višnjevac, A.; Padrón, J. M.; Todorović, T. R.; Filipović, N. R. Structural, Antioxidant, Antiproliferative and In‒silico Study of Pyridine-Based Hydrazonyl‒selenazoles and Their Sulphur Isosteres. Journal of Molecular Structure 2021, 1240, 130512. https://doi.org/10.1016/j.molstruc.2021.130512.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4540
ER  - 
@misc{
author = "Araškov, Jovana B. and Nikolić, Milan and Armaković, Stevan and Armaković, Sanja and Rodić, Marko and Višnjevac, Aleksandar and Padrón, José M. and Todorović, Tamara and Filipović, Nenad R.",
year = "2021",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Supplementary data for the article: Araškov, J. B.; Nikolić, M.; Armaković, S.; Armaković, S.; Rodić, M.; Višnjevac, A.; Padrón, J. M.; Todorović, T. R.; Filipović, N. R. Structural, Antioxidant, Antiproliferative and In‒silico Study of Pyridine-Based Hydrazonyl‒selenazoles and Their Sulphur Isosteres. Journal of Molecular Structure 2021, 1240, 130512. https://doi.org/10.1016/j.molstruc.2021.130512.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4540"
}
Araškov, J. B., Nikolić, M., Armaković, S., Armaković, S., Rodić, M., Višnjevac, A., Padrón, J. M., Todorović, T.,& Filipović, N. R.. (2021). Supplementary data for the article: Araškov, J. B.; Nikolić, M.; Armaković, S.; Armaković, S.; Rodić, M.; Višnjevac, A.; Padrón, J. M.; Todorović, T. R.; Filipović, N. R. Structural, Antioxidant, Antiproliferative and In‒silico Study of Pyridine-Based Hydrazonyl‒selenazoles and Their Sulphur Isosteres. Journal of Molecular Structure 2021, 1240, 130512. https://doi.org/10.1016/j.molstruc.2021.130512.. in Journal of Molecular Structure
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4540
Araškov JB, Nikolić M, Armaković S, Armaković S, Rodić M, Višnjevac A, Padrón JM, Todorović T, Filipović NR. Supplementary data for the article: Araškov, J. B.; Nikolić, M.; Armaković, S.; Armaković, S.; Rodić, M.; Višnjevac, A.; Padrón, J. M.; Todorović, T. R.; Filipović, N. R. Structural, Antioxidant, Antiproliferative and In‒silico Study of Pyridine-Based Hydrazonyl‒selenazoles and Their Sulphur Isosteres. Journal of Molecular Structure 2021, 1240, 130512. https://doi.org/10.1016/j.molstruc.2021.130512.. in Journal of Molecular Structure. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4540 .
Araškov, Jovana B., Nikolić, Milan, Armaković, Stevan, Armaković, Sanja, Rodić, Marko, Višnjevac, Aleksandar, Padrón, José M., Todorović, Tamara, Filipović, Nenad R., "Supplementary data for the article: Araškov, J. B.; Nikolić, M.; Armaković, S.; Armaković, S.; Rodić, M.; Višnjevac, A.; Padrón, J. M.; Todorović, T. R.; Filipović, N. R. Structural, Antioxidant, Antiproliferative and In‒silico Study of Pyridine-Based Hydrazonyl‒selenazoles and Their Sulphur Isosteres. Journal of Molecular Structure 2021, 1240, 130512. https://doi.org/10.1016/j.molstruc.2021.130512." in Journal of Molecular Structure (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4540 .

Supplementary data for the article: Šunderić, M.; Vasović, T.; Milčić, M.; Miljević, Č.; Nedić, O.; Nikolić, M. R.; Gligorijević, N. Antipsychotic Clozapine Binding to Alpha-2-Macroglobulin Protects Interacting Partners against Oxidation and Preserves the Anti-Proteinase Activity of the Protein. International Journal of Biological Macromolecules 2021, 183, 502–512. https://doi.org/10.1016/j.ijbiomac.2021.04.155.

Šunderić, Miloš; Vasović, Tamara; Milčić, Miloš K.; Miljević, Čedo; Nedić, Olgica; Nikolić, Milan; Gligorijević, Nikola

(Elsevier, 2021)

TY  - DATA
AU  - Šunderić, Miloš
AU  - Vasović, Tamara
AU  - Milčić, Miloš K.
AU  - Miljević, Čedo
AU  - Nedić, Olgica
AU  - Nikolić, Milan
AU  - Gligorijević, Nikola
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0141813021009284
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4541
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Supplementary data for the article: Šunderić, M.; Vasović, T.; Milčić, M.; Miljević, Č.; Nedić, O.; Nikolić, M. R.; Gligorijević, N. Antipsychotic Clozapine Binding to Alpha-2-Macroglobulin Protects Interacting Partners against Oxidation and Preserves the Anti-Proteinase Activity of the Protein. International Journal of Biological Macromolecules 2021, 183, 502–512. https://doi.org/10.1016/j.ijbiomac.2021.04.155.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4541
ER  - 
@misc{
author = "Šunderić, Miloš and Vasović, Tamara and Milčić, Miloš K. and Miljević, Čedo and Nedić, Olgica and Nikolić, Milan and Gligorijević, Nikola",
year = "2021",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Supplementary data for the article: Šunderić, M.; Vasović, T.; Milčić, M.; Miljević, Č.; Nedić, O.; Nikolić, M. R.; Gligorijević, N. Antipsychotic Clozapine Binding to Alpha-2-Macroglobulin Protects Interacting Partners against Oxidation and Preserves the Anti-Proteinase Activity of the Protein. International Journal of Biological Macromolecules 2021, 183, 502–512. https://doi.org/10.1016/j.ijbiomac.2021.04.155.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4541"
}
Šunderić, M., Vasović, T., Milčić, M. K., Miljević, Č., Nedić, O., Nikolić, M.,& Gligorijević, N.. (2021). Supplementary data for the article: Šunderić, M.; Vasović, T.; Milčić, M.; Miljević, Č.; Nedić, O.; Nikolić, M. R.; Gligorijević, N. Antipsychotic Clozapine Binding to Alpha-2-Macroglobulin Protects Interacting Partners against Oxidation and Preserves the Anti-Proteinase Activity of the Protein. International Journal of Biological Macromolecules 2021, 183, 502–512. https://doi.org/10.1016/j.ijbiomac.2021.04.155.. in International Journal of Biological Macromolecules
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4541
Šunderić M, Vasović T, Milčić MK, Miljević Č, Nedić O, Nikolić M, Gligorijević N. Supplementary data for the article: Šunderić, M.; Vasović, T.; Milčić, M.; Miljević, Č.; Nedić, O.; Nikolić, M. R.; Gligorijević, N. Antipsychotic Clozapine Binding to Alpha-2-Macroglobulin Protects Interacting Partners against Oxidation and Preserves the Anti-Proteinase Activity of the Protein. International Journal of Biological Macromolecules 2021, 183, 502–512. https://doi.org/10.1016/j.ijbiomac.2021.04.155.. in International Journal of Biological Macromolecules. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4541 .
Šunderić, Miloš, Vasović, Tamara, Milčić, Miloš K., Miljević, Čedo, Nedić, Olgica, Nikolić, Milan, Gligorijević, Nikola, "Supplementary data for the article: Šunderić, M.; Vasović, T.; Milčić, M.; Miljević, Č.; Nedić, O.; Nikolić, M. R.; Gligorijević, N. Antipsychotic Clozapine Binding to Alpha-2-Macroglobulin Protects Interacting Partners against Oxidation and Preserves the Anti-Proteinase Activity of the Protein. International Journal of Biological Macromolecules 2021, 183, 502–512. https://doi.org/10.1016/j.ijbiomac.2021.04.155." in International Journal of Biological Macromolecules (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4541 .

Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2

Đukić, Teodora; Mladenović, Maja; Stanić-Vučinić, Dragana; Radosavljević, Jelena; Smiljanić, Katarina; Sabljić, Ljiljana; Dević, Marija; Ćujić, Danica; Vasović, Tamara; Simović, Ana; Radomirović, Mirjana Ž.; Ćirković-Veličković, Tanja

(Elsevier, 2021)

TY  - JOUR
AU  - Đukić, Teodora
AU  - Mladenović, Maja
AU  - Stanić-Vučinić, Dragana
AU  - Radosavljević, Jelena
AU  - Smiljanić, Katarina
AU  - Sabljić, Ljiljana
AU  - Dević, Marija
AU  - Ćujić, Danica
AU  - Vasović, Tamara
AU  - Simović, Ana
AU  - Radomirović, Mirjana Ž.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4330
AB  - Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production.

SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58–419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically.

Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of β-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively).

Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection.
PB  - Elsevier
T2  - Virology journal
T1  - Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2
VL  - 557
SP  - 15
EP  - 22
DO  - 10.1016/j.virol.2021.01.004
ER  - 
@article{
author = "Đukić, Teodora and Mladenović, Maja and Stanić-Vučinić, Dragana and Radosavljević, Jelena and Smiljanić, Katarina and Sabljić, Ljiljana and Dević, Marija and Ćujić, Danica and Vasović, Tamara and Simović, Ana and Radomirović, Mirjana Ž. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production.

SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58–419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically.

Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of β-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively).

Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection.",
publisher = "Elsevier",
journal = "Virology journal",
title = "Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2",
volume = "557",
pages = "15-22",
doi = "10.1016/j.virol.2021.01.004"
}
Đukić, T., Mladenović, M., Stanić-Vučinić, D., Radosavljević, J., Smiljanić, K., Sabljić, L., Dević, M., Ćujić, D., Vasović, T., Simović, A., Radomirović, M. Ž.,& Ćirković-Veličković, T.. (2021). Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2. in Virology journal
Elsevier., 557, 15-22.
https://doi.org/10.1016/j.virol.2021.01.004
Đukić T, Mladenović M, Stanić-Vučinić D, Radosavljević J, Smiljanić K, Sabljić L, Dević M, Ćujić D, Vasović T, Simović A, Radomirović MŽ, Ćirković-Veličković T. Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2. in Virology journal. 2021;557:15-22.
doi:10.1016/j.virol.2021.01.004 .
Đukić, Teodora, Mladenović, Maja, Stanić-Vučinić, Dragana, Radosavljević, Jelena, Smiljanić, Katarina, Sabljić, Ljiljana, Dević, Marija, Ćujić, Danica, Vasović, Tamara, Simović, Ana, Radomirović, Mirjana Ž., Ćirković-Veličković, Tanja, "Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2" in Virology journal, 557 (2021):15-22,
https://doi.org/10.1016/j.virol.2021.01.004 . .
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Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2

Đukić, Teodora; Mladenović, Maja; Stanić-Vučinić, Dragana; Radosavljević, Jelena; Smiljanić, Katarina; Sabljić, Ljiljana; Dević, Marija; Ćujić, Danica; Vasović, Tamara; Simović, Ana; Radomirović, Mirjana Ž.; Ćirković-Veličković, Tanja

(Elsevier, 2021)

TY  - JOUR
AU  - Đukić, Teodora
AU  - Mladenović, Maja
AU  - Stanić-Vučinić, Dragana
AU  - Radosavljević, Jelena
AU  - Smiljanić, Katarina
AU  - Sabljić, Ljiljana
AU  - Dević, Marija
AU  - Ćujić, Danica
AU  - Vasović, Tamara
AU  - Simović, Ana
AU  - Radomirović, Mirjana Ž.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4331
AB  - Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production.SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58–419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically.Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of β-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively).Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection.
PB  - Elsevier
T2  - Virology journal
T1  - Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2
VL  - 557
SP  - 15
EP  - 22
DO  - 10.1016/j.virol.2021.01.004
ER  - 
@article{
author = "Đukić, Teodora and Mladenović, Maja and Stanić-Vučinić, Dragana and Radosavljević, Jelena and Smiljanić, Katarina and Sabljić, Ljiljana and Dević, Marija and Ćujić, Danica and Vasović, Tamara and Simović, Ana and Radomirović, Mirjana Ž. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production.SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58–419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically.Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of β-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively).Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection.",
publisher = "Elsevier",
journal = "Virology journal",
title = "Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2",
volume = "557",
pages = "15-22",
doi = "10.1016/j.virol.2021.01.004"
}
Đukić, T., Mladenović, M., Stanić-Vučinić, D., Radosavljević, J., Smiljanić, K., Sabljić, L., Dević, M., Ćujić, D., Vasović, T., Simović, A., Radomirović, M. Ž.,& Ćirković-Veličković, T.. (2021). Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2. in Virology journal
Elsevier., 557, 15-22.
https://doi.org/10.1016/j.virol.2021.01.004
Đukić T, Mladenović M, Stanić-Vučinić D, Radosavljević J, Smiljanić K, Sabljić L, Dević M, Ćujić D, Vasović T, Simović A, Radomirović MŽ, Ćirković-Veličković T. Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2. in Virology journal. 2021;557:15-22.
doi:10.1016/j.virol.2021.01.004 .
Đukić, Teodora, Mladenović, Maja, Stanić-Vučinić, Dragana, Radosavljević, Jelena, Smiljanić, Katarina, Sabljić, Ljiljana, Dević, Marija, Ćujić, Danica, Vasović, Tamara, Simović, Ana, Radomirović, Mirjana Ž., Ćirković-Veličković, Tanja, "Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2" in Virology journal, 557 (2021):15-22,
https://doi.org/10.1016/j.virol.2021.01.004 . .
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