Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds

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Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (en)
Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (sr)
Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (sr_RS)
Authors

Publications

Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005

Banjanac, Tijana; Dragičević, Milan; Šiler, Branislav; Gašić, Uroš M.; Bohanec, Borut; Nestorović Živković, Jasmina; Trifunović, Snežana S.; Mišić, Danijela

TY  - BOOK
AU  - Banjanac, Tijana
AU  - Dragičević, Milan
AU  - Šiler, Branislav
AU  - Gašić, Uroš M.
AU  - Bohanec, Borut
AU  - Nestorović Živković, Jasmina
AU  - Trifunović, Snežana S.
AU  - Mišić, Danijela
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3098
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Phytochemistry
T1  - Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005
ER  - 
@book{
author = "Banjanac, Tijana and Dragičević, Milan and Šiler, Branislav and Gašić, Uroš M. and Bohanec, Borut and Nestorović Živković, Jasmina and Trifunović, Snežana S. and Mišić, Danijela",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3098",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Phytochemistry",
title = "Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005"
}
Banjanac, T., Dragičević, M., Šiler, B., Gašić, U. M., Bohanec, B., Nestorović Živković, J., Trifunović, S. S.,& Mišić, D.Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005.
Phytochemistry
Pergamon-Elsevier Science Ltd, Oxford..
Banjanac T, Dragičević M, Šiler B, Gašić UM, Bohanec B, Nestorović Živković J, Trifunović SS, Mišić D. Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005. Phytochemistry.
Banjanac Tijana, Dragičević Milan, Šiler Branislav, Gašić Uroš M., Bohanec Borut, Nestorović Živković Jasmina, Trifunović Snežana S., Mišić Danijela, "Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005" Phytochemistry

Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars

Stikić, Radmila I.; Milinčić, Danijel D.; Kostić, Aleksandar Ž.; Jovanović, Zorica; Gašić, Uroš M.; Tešić, Živoslav Lj.; Djordjević, Nataša Z.; Savić, Sladjana K.; Czekus, Borisz G.; Pešić, Mirjana B.

(Wiley-Blackwell, 2020)

TY  - JOUR
AU  - Stikić, Radmila I.
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Jovanović, Zorica
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana K.
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana B.
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4047
AB  - Background and objectives: Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings: Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions: The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty: The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.
PB  - Wiley-Blackwell
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
VL  - 97
IS  - 3
SP  - 626
EP  - 633
DO  - 10.1002/cche.10278
ER  - 
@article{
author = "Stikić, Radmila I. and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Jovanović, Zorica and Gašić, Uroš M. and Tešić, Živoslav Lj. and Djordjević, Nataša Z. and Savić, Sladjana K. and Czekus, Borisz G. and Pešić, Mirjana B.",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/4047",
abstract = "Background and objectives: Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings: Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions: The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty: The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.",
publisher = "Wiley-Blackwell",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
volume = "97",
number = "3",
pages = "626-633",
doi = "10.1002/cche.10278"
}
Stikić, R. I., Milinčić, D. D., Kostić, A. Ž., Jovanović, Z., Gašić, U. M., Tešić, Ž. Lj., Djordjević, N. Z., Savić, S. K., Czekus, B. G.,& Pešić, M. B. (2020). Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars.
Cereal Chemistry
Wiley-Blackwell., 97(3), 626-633.
https://doi.org/10.1002/cche.10278
Stikić RI, Milinčić DD, Kostić AŽ, Jovanović Z, Gašić UM, Tešić ŽL, Djordjević NZ, Savić SK, Czekus BG, Pešić MB. Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. Cereal Chemistry. 2020;97(3):626-633
Stikić Radmila I., Milinčić Danijel D., Kostić Aleksandar Ž., Jovanović Zorica, Gašić Uroš M., Tešić Živoslav Lj., Djordjević Nataša Z., Savić Sladjana K., Czekus Borisz G., Pešić Mirjana B., "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" Cereal Chemistry, 97, no. 3 (2020):626-633,
https://doi.org/10.1002/cche.10278 .
1
3
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2

Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid

Katanić-Stanković, Jelena; Srećković, Nikola; Mišić, Danijela; Gašić, Uroš M.; Imbimbo, Paola; Monti, Daria M.; Mihailović, Vladimir

(Elsevier, 2020)

TY  - JOUR
AU  - Katanić-Stanković, Jelena
AU  - Srećković, Nikola
AU  - Mišić, Danijela
AU  - Gašić, Uroš M.
AU  - Imbimbo, Paola
AU  - Monti, Daria M.
AU  - Mihailović, Vladimir
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3791
AB  - The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid
VL  - 143
SP  - 111932
DO  - 10.1016/j.indcrop.2019.111932
ER  - 
@article{
author = "Katanić-Stanković, Jelena and Srećković, Nikola and Mišić, Danijela and Gašić, Uroš M. and Imbimbo, Paola and Monti, Daria M. and Mihailović, Vladimir",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3791",
abstract = "The plants from genus Salvia, as one of the largest genus in Lamiaceae family, are frequently in use for various purposes, as foods, in cosmetic industry, or in traditional and official medicine. Salvia verticillata L. (liliac sage) is one of sidelined sage species with potential bioactivity, reported in traditional medicine. The aim of this study was to acquire a phytochemical profile of the methanol extract obtained from S. verticillata aerial parts and to evaluate its antioxidant, antimicrobial, and biocompatibility potential. Characteristic compounds of the genus Salvia, such as rosmarinic and caffeic acids, along with their derivatives (e.g. salvianolic and yunnaneic acids isomers) and flavonoids, have been identified by ultrahigh-performance Orbitrap metabolomic fingerprinting as the main phenolic metabolites in S. verticillata. The extract displayed moderate antimicrobial properties and significant antioxidant potential, with the half maximal inhibitory concentration values (IC50) ranging from 33 to 73 μg/mL. Importantly, full biocompatibility of the extract with eukaryotic cell lines was observed up to 72 h. The obtained results revealed the presence of polyphenolic bioactive compounds in S. verticillata extract with promising antioxidant potential and significant biocompatibility. In this regard, S. verticillata can find new perspectives of application as a food ingredient, in cosmetic and pharmaceutical industries, as it represents a valuable source of compounds with prominent health properties, with a special focus on rosmarinic acid.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid",
volume = "143",
pages = "111932",
doi = "10.1016/j.indcrop.2019.111932"
}
Katanić-Stanković, J., Srećković, N., Mišić, D., Gašić, U. M., Imbimbo, P., Monti, D. M.,& Mihailović, V. (2020). Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid.
Industrial Crops and Products
Elsevier., 143, 111932.
https://doi.org/10.1016/j.indcrop.2019.111932
Katanić-Stanković J, Srećković N, Mišić D, Gašić UM, Imbimbo P, Monti DM, Mihailović V. Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid. Industrial Crops and Products. 2020;143:111932
Katanić-Stanković Jelena, Srećković Nikola, Mišić Danijela, Gašić Uroš M., Imbimbo Paola, Monti Daria M., Mihailović Vladimir, "Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid" Industrial Crops and Products, 143 (2020):111932,
https://doi.org/10.1016/j.indcrop.2019.111932 .
12
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Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro

Šuković, Danijela; Knežević, Bojana; Gašić, Uroš M.; Sredojević, Milica; Ćirić, Ivanka; Todić, Slavica; Mutić, Jelena; Tešić, Živoslav Lj.

(MDPI, 2020)

TY  - JOUR
AU  - Šuković, Danijela
AU  - Knežević, Bojana
AU  - Gašić, Uroš M.
AU  - Sredojević, Milica
AU  - Ćirić, Ivanka
AU  - Todić, Slavica
AU  - Mutić, Jelena
AU  - Tešić, Živoslav Lj.
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3879
AB  - Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.
PB  - MDPI
T2  - Foods
T1  - Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro
VL  - 9
IS  - 2
SP  - 1
EP  - 24
DO  - 10.3390/foods9020138
ER  - 
@article{
author = "Šuković, Danijela and Knežević, Bojana and Gašić, Uroš M. and Sredojević, Milica and Ćirić, Ivanka and Todić, Slavica and Mutić, Jelena and Tešić, Živoslav Lj.",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3879",
abstract = "Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.",
publisher = "MDPI",
journal = "Foods",
title = "Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro",
volume = "9",
number = "2",
pages = "1-24",
doi = "10.3390/foods9020138"
}
Šuković, D., Knežević, B., Gašić, U. M., Sredojević, M., Ćirić, I., Todić, S., Mutić, J.,& Tešić, Ž. Lj. (2020). Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro.
Foods
MDPI., 9(2), 1-24.
https://doi.org/10.3390/foods9020138
Šuković D, Knežević B, Gašić UM, Sredojević M, Ćirić I, Todić S, Mutić J, Tešić ŽL. Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro. Foods. 2020;9(2):1-24
Šuković Danijela, Knežević Bojana, Gašić Uroš M., Sredojević Milica, Ćirić Ivanka, Todić Slavica, Mutić Jelena, Tešić Živoslav Lj., "Phenolic profiles of leaves, grapes and wine of grapevine variety vranac (Vitis vinifera L.) from Montenegro" Foods, 9, no. 2 (2020):1-24,
https://doi.org/10.3390/foods9020138 .
1
10
4
10

In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity

Božunović, Jelena; Živković, Suzana; Gašić, Uroš M.; Glamočlija, Jasmina; Ćirić, Ana D.; Matekalo, Dragana; Šiler, Branislav; Soković, Marina; Tešić, Živoslav Lj.; Mišić, Danijela

(Elsevier Science Bv, Amsterdam, 2018)

TY  - JOUR
AU  - Božunović, Jelena
AU  - Živković, Suzana
AU  - Gašić, Uroš M.
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana D.
AU  - Matekalo, Dragana
AU  - Šiler, Branislav
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Mišić, Danijela
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2579
AB  - The present study was principally aimed at ascertaining the differences in metabolomics profiles and biological activities between non-hydrolyzed (ME) and hydrolyzed methanol extract (HME) of Centaurium erythraea Rafn. UHPLC-MS/MS Orbitrap analysis showed that the enzymatic hydrolysis of the extract caused changes in beta-Dglycoside/aglycone ratio of both flavonoid and secoiridoid compounds. UHPLC/DAD/ + HESI - qqqMS characterization and/or quantification of secoiridoid glucosides (SGs) and their aglycones in both ME and HME revealed gentiopicral and erythrocentaurin as the major aglycones, the same metabolic products which appear after the hydrolysis of pure swertiamarin, the dominant secoiridoid glucoside of C. erythraea. SGs played an antioxidant role only in ABTS assay, whilst the remarkable antioxidant potential of C. erythraea methanol extract is ascribed chiefly to phenolics detected in it. Interestingly, antioxidant activities of swertiamarin and sweroside recorded in ABTS assay increased after the compounds have been hydrolyzed, which highlighted their possible antioxidant role during ingestion. Strong antimicrobial activities of ME and HME against a vast array of pathogens, which exceed the effects of the reference antibiotics and antimycotics, largely depended on the amount of secoiridoids in either of the glycosylation forms. Extracts and pure secoiridoids were especially effective against most of the tested Penicillium species. On the other hand, P. funiculosum has evolved an efficient mechanism of detoxification of sub-lethal concentrations of secoiridoid glucosides, involving their biotransformation and complete digestion. The presented findings will contribute to clarify the fate and role of the SGs after C. erythraea ingestion within in vivo systems, and to further promote this remarkable plant as a food preservation additive with significant health benefits.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity
VL  - 111
SP  - 705
EP  - 721
DO  - 10.1016/j.indcrop.2017.11.040
ER  - 
@article{
author = "Božunović, Jelena and Živković, Suzana and Gašić, Uroš M. and Glamočlija, Jasmina and Ćirić, Ana D. and Matekalo, Dragana and Šiler, Branislav and Soković, Marina and Tešić, Živoslav Lj. and Mišić, Danijela",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2579",
abstract = "The present study was principally aimed at ascertaining the differences in metabolomics profiles and biological activities between non-hydrolyzed (ME) and hydrolyzed methanol extract (HME) of Centaurium erythraea Rafn. UHPLC-MS/MS Orbitrap analysis showed that the enzymatic hydrolysis of the extract caused changes in beta-Dglycoside/aglycone ratio of both flavonoid and secoiridoid compounds. UHPLC/DAD/ + HESI - qqqMS characterization and/or quantification of secoiridoid glucosides (SGs) and their aglycones in both ME and HME revealed gentiopicral and erythrocentaurin as the major aglycones, the same metabolic products which appear after the hydrolysis of pure swertiamarin, the dominant secoiridoid glucoside of C. erythraea. SGs played an antioxidant role only in ABTS assay, whilst the remarkable antioxidant potential of C. erythraea methanol extract is ascribed chiefly to phenolics detected in it. Interestingly, antioxidant activities of swertiamarin and sweroside recorded in ABTS assay increased after the compounds have been hydrolyzed, which highlighted their possible antioxidant role during ingestion. Strong antimicrobial activities of ME and HME against a vast array of pathogens, which exceed the effects of the reference antibiotics and antimycotics, largely depended on the amount of secoiridoids in either of the glycosylation forms. Extracts and pure secoiridoids were especially effective against most of the tested Penicillium species. On the other hand, P. funiculosum has evolved an efficient mechanism of detoxification of sub-lethal concentrations of secoiridoid glucosides, involving their biotransformation and complete digestion. The presented findings will contribute to clarify the fate and role of the SGs after C. erythraea ingestion within in vivo systems, and to further promote this remarkable plant as a food preservation additive with significant health benefits.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity",
volume = "111",
pages = "705-721",
doi = "10.1016/j.indcrop.2017.11.040"
}
Božunović, J., Živković, S., Gašić, U. M., Glamočlija, J., Ćirić, A. D., Matekalo, D., Šiler, B., Soković, M., Tešić, Ž. Lj.,& Mišić, D. (2018). In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity.
Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 111, 705-721.
https://doi.org/10.1016/j.indcrop.2017.11.040
Božunović J, Živković S, Gašić UM, Glamočlija J, Ćirić AD, Matekalo D, Šiler B, Soković M, Tešić ŽL, Mišić D. In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity. Industrial Crops and Products. 2018;111:705-721
Božunović Jelena, Živković Suzana, Gašić Uroš M., Glamočlija Jasmina, Ćirić Ana D., Matekalo Dragana, Šiler Branislav, Soković Marina, Tešić Živoslav Lj., Mišić Danijela, "In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity" Industrial Crops and Products, 111 (2018):705-721,
https://doi.org/10.1016/j.indcrop.2017.11.040 .
15
12
15

Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers

Banjanac, Tijana; Dragičević, Milan; Šiler, Branislav; Gašić, Uroš M.; Bohanec, Borut; Nestorović Živković, Jasmina; Trifunović, Snežana S.; Mišić, Danijela

(Pergamon-Elsevier Science Ltd, Oxford, 2017)

TY  - JOUR
AU  - Banjanac, Tijana
AU  - Dragičević, Milan
AU  - Šiler, Branislav
AU  - Gašić, Uroš M.
AU  - Bohanec, Borut
AU  - Nestorović Živković, Jasmina
AU  - Trifunović, Snežana S.
AU  - Mišić, Danijela
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2477
AB  - Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Phytochemistry
T1  - Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers
VL  - 140
SP  - 27
EP  - 44
DO  - 10.1016/j.phytochem.2017.04.005
ER  - 
@article{
author = "Banjanac, Tijana and Dragičević, Milan and Šiler, Branislav and Gašić, Uroš M. and Bohanec, Borut and Nestorović Živković, Jasmina and Trifunović, Snežana S. and Mišić, Danijela",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2477",
abstract = "Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Phytochemistry",
title = "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers",
volume = "140",
pages = "27-44",
doi = "10.1016/j.phytochem.2017.04.005"
}
Banjanac, T., Dragičević, M., Šiler, B., Gašić, U. M., Bohanec, B., Nestorović Živković, J., Trifunović, S. S.,& Mišić, D. (2017). Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers.
Phytochemistry
Pergamon-Elsevier Science Ltd, Oxford., 140, 27-44.
https://doi.org/10.1016/j.phytochem.2017.04.005
Banjanac T, Dragičević M, Šiler B, Gašić UM, Bohanec B, Nestorović Živković J, Trifunović SS, Mišić D. Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. Phytochemistry. 2017;140:27-44
Banjanac Tijana, Dragičević Milan, Šiler Branislav, Gašić Uroš M., Bohanec Borut, Nestorović Živković Jasmina, Trifunović Snežana S., Mišić Danijela, "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers" Phytochemistry, 140 (2017):27-44,
https://doi.org/10.1016/j.phytochem.2017.04.005 .
1
25
22
24

Anti-encephalitogenic effects of cucumber leaf extract

Jevtic, Bojan; Djedovic, Neda; Stanisavljevic, Suzana; Gašić, Uroš M.; Mišić, Danijela; Despotovic, Jovana; Samardžić, Jelena; Miljkovic, Djordje; Timotijević, Gordana

(Elsevier Science Bv, Amsterdam, 2017)

TY  - JOUR
AU  - Jevtic, Bojan
AU  - Djedovic, Neda
AU  - Stanisavljevic, Suzana
AU  - Gašić, Uroš M.
AU  - Mišić, Danijela
AU  - Despotovic, Jovana
AU  - Samardžić, Jelena
AU  - Miljkovic, Djordje
AU  - Timotijević, Gordana
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2537
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
VL  - 37
SP  - 249
EP  - 262
DO  - 10.1016/j.jff.2017.07.060
ER  - 
@article{
author = "Jevtic, Bojan and Djedovic, Neda and Stanisavljevic, Suzana and Gašić, Uroš M. and Mišić, Danijela and Despotovic, Jovana and Samardžić, Jelena and Miljkovic, Djordje and Timotijević, Gordana",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2537",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
volume = "37",
pages = "249-262",
doi = "10.1016/j.jff.2017.07.060"
}
Jevtic, B., Djedovic, N., Stanisavljevic, S., Gašić, U. M., Mišić, D., Despotovic, J., Samardžić, J., Miljkovic, D.,& Timotijević, G. (2017). Anti-encephalitogenic effects of cucumber leaf extract.
Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060
Jevtic B, Djedovic N, Stanisavljevic S, Gašić UM, Mišić D, Despotovic J, Samardžić J, Miljkovic D, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. Journal of Functional Foods. 2017;37:249-262
Jevtic Bojan, Djedovic Neda, Stanisavljevic Suzana, Gašić Uroš M., Mišić Danijela, Despotovic Jovana, Samardžić Jelena, Miljkovic Djordje, Timotijević Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 .
3
2

Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052

Pavlović, Aleksandra V.; Papetti, Adele; Dabić-Zagorac, Dragana; Gašić, Uroš M.; Mišić, Danijela; Tešić, Živoslav Lj.; Natić, Maja

(Elsevier Science Bv, Amsterdam, 2016)

TY  - BOOK
AU  - Pavlović, Aleksandra V.
AU  - Papetti, Adele
AU  - Dabić-Zagorac, Dragana
AU  - Gašić, Uroš M.
AU  - Mišić, Danijela
AU  - Tešić, Živoslav Lj.
AU  - Natić, Maja
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3594
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052
ER  - 
@book{
author = "Pavlović, Aleksandra V. and Papetti, Adele and Dabić-Zagorac, Dragana and Gašić, Uroš M. and Mišić, Danijela and Tešić, Živoslav Lj. and Natić, Maja",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3594",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052"
}
Pavlović, A. V., Papetti, A., Dabić-Zagorac, D., Gašić, U. M., Mišić, D., Tešić, Ž. Lj.,& Natić, M. (2016). Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052.
Industrial Crops and Products
Elsevier Science Bv, Amsterdam..
Pavlović AV, Papetti A, Dabić-Zagorac D, Gašić UM, Mišić D, Tešić ŽL, Natić M. Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052. Industrial Crops and Products. 2016;
Pavlović Aleksandra V., Papetti Adele, Dabić-Zagorac Dragana, Gašić Uroš M., Mišić Danijela, Tešić Živoslav Lj., Natić Maja, "Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052" Industrial Crops and Products (2016)

Phenolics composition of leaf extracts of raspberry and blackberry cultivars grown in Serbia

Pavlović, Aleksandra V.; Papetti, Adele; Dabić-Zagorac, Dragana; Gašić, Uroš M.; Mišić, Danijela; Tešić, Živoslav Lj.; Natić, Maja

(Elsevier Science Bv, Amsterdam, 2016)

TY  - JOUR
AU  - Pavlović, Aleksandra V.
AU  - Papetti, Adele
AU  - Dabić-Zagorac, Dragana
AU  - Gašić, Uroš M.
AU  - Mišić, Danijela
AU  - Tešić, Živoslav Lj.
AU  - Natić, Maja
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2257
AB  - The polyphenolic profiles of leaves from three raspberry (Rubus idaeus L.) cultivars ('Willamette', 'Tulameen', 'Meeker') and one blackberry (Rubus fruticosus L) cultivar, ('Cganska Bestrna') grown in West Serbia were evaluated. To characterise the extracts, two hyphenated techniques were employed. Ultra-high performance liquid chromatography (UHPLC) coupled with an LTQOrbiTrap mass spectrometer was used to identify polyphenolics. A rapid and accurate procedure in negative ion mode using a gradient elution within 15 min was established. This allowed us to identify 83 compounds, mostly hydroxycinnamic acid esters, flavonol glycosides, flavan-3-ols and ellagic acid derivatives. Hydroxybenzoic acid derivatives were identified in all investigated samples. Quantification was achieved with a time selected reaction monitoring (tSRM) method using UHPLC coupled with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Among 23 compounds that were quantitated using available standards, ellagic acid was found to be the main phenolic acid in the Rubus leaves [ranging from 1574 to 2875 mg kg(-1) dry weight (DW)]. The raspberry leaves had higher contents of catechin, epigallocatechin gallate, rutin, ferulic acid, chlorogenic acid and p-hydroxybenzoic acid than the blackberry leaf sample. On the other hand, there was more caffeic acid and aesculin in the blackberry leaf sample than in raspberry leaves. All leaves were characterized by a high total phenolic content (84.64-144.20 mg GAEg(-1) DW), favourable radical scavenging capacity (38.05-83.77%), adequate ferric ion reducing power (59.74-92.80 mu mol L-1 of an ascorbic acid solution), good superoxide anion radical scavenging activity (28.43-39.87%), but low metal chelating ability (1.55-8.86%). To our best knowledge, this is the first report describing the phenolic profile of Cacanska Bestrna' leaves. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolics composition of leaf extracts of raspberry and blackberry cultivars grown in Serbia
VL  - 87
SP  - 304
EP  - 314
DO  - 10.1016/j.indcrop.2016.04.052
ER  - 
@article{
author = "Pavlović, Aleksandra V. and Papetti, Adele and Dabić-Zagorac, Dragana and Gašić, Uroš M. and Mišić, Danijela and Tešić, Živoslav Lj. and Natić, Maja",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2257",
abstract = "The polyphenolic profiles of leaves from three raspberry (Rubus idaeus L.) cultivars ('Willamette', 'Tulameen', 'Meeker') and one blackberry (Rubus fruticosus L) cultivar, ('Cganska Bestrna') grown in West Serbia were evaluated. To characterise the extracts, two hyphenated techniques were employed. Ultra-high performance liquid chromatography (UHPLC) coupled with an LTQOrbiTrap mass spectrometer was used to identify polyphenolics. A rapid and accurate procedure in negative ion mode using a gradient elution within 15 min was established. This allowed us to identify 83 compounds, mostly hydroxycinnamic acid esters, flavonol glycosides, flavan-3-ols and ellagic acid derivatives. Hydroxybenzoic acid derivatives were identified in all investigated samples. Quantification was achieved with a time selected reaction monitoring (tSRM) method using UHPLC coupled with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Among 23 compounds that were quantitated using available standards, ellagic acid was found to be the main phenolic acid in the Rubus leaves [ranging from 1574 to 2875 mg kg(-1) dry weight (DW)]. The raspberry leaves had higher contents of catechin, epigallocatechin gallate, rutin, ferulic acid, chlorogenic acid and p-hydroxybenzoic acid than the blackberry leaf sample. On the other hand, there was more caffeic acid and aesculin in the blackberry leaf sample than in raspberry leaves. All leaves were characterized by a high total phenolic content (84.64-144.20 mg GAEg(-1) DW), favourable radical scavenging capacity (38.05-83.77%), adequate ferric ion reducing power (59.74-92.80 mu mol L-1 of an ascorbic acid solution), good superoxide anion radical scavenging activity (28.43-39.87%), but low metal chelating ability (1.55-8.86%). To our best knowledge, this is the first report describing the phenolic profile of Cacanska Bestrna' leaves. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolics composition of leaf extracts of raspberry and blackberry cultivars grown in Serbia",
volume = "87",
pages = "304-314",
doi = "10.1016/j.indcrop.2016.04.052"
}
Pavlović, A. V., Papetti, A., Dabić-Zagorac, D., Gašić, U. M., Mišić, D., Tešić, Ž. Lj.,& Natić, M. (2016). Phenolics composition of leaf extracts of raspberry and blackberry cultivars grown in Serbia.
Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 87, 304-314.
https://doi.org/10.1016/j.indcrop.2016.04.052
Pavlović AV, Papetti A, Dabić-Zagorac D, Gašić UM, Mišić D, Tešić ŽL, Natić M. Phenolics composition of leaf extracts of raspberry and blackberry cultivars grown in Serbia. Industrial Crops and Products. 2016;87:304-314
Pavlović Aleksandra V., Papetti Adele, Dabić-Zagorac Dragana, Gašić Uroš M., Mišić Danijela, Tešić Živoslav Lj., Natić Maja, "Phenolics composition of leaf extracts of raspberry and blackberry cultivars grown in Serbia" Industrial Crops and Products, 87 (2016):304-314,
https://doi.org/10.1016/j.indcrop.2016.04.052 .
32
30
31

Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies

Mišić, Danijela; Šiler, Branislav; Gašić, Uroš M.; Avramov, Stevan N.; Živković, Suzana; Nestorović Živković, Jasmina; Milutinovic, Milica; Tešić, Živoslav Lj.

(Wiley-Blackwell, Hoboken, 2015)

TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Gašić, Uroš M.
AU  - Avramov, Stevan N.
AU  - Živković, Suzana
AU  - Nestorović Živković, Jasmina
AU  - Milutinovic, Milica
AU  - Tešić, Živoslav Lj.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1663
AB  - Introduction - Nepeta species contain a variety of secondary metabolites, including iridoid monoterpenes - nepetalactones and phenolic acids - that are considered the main bioactive constituents. This work represents the first attempt to comparatively explore variations in these two major groups of secondary metabolites within the genus. Objective - To develop an efficient analytical methodology for simultaneous analysis of nepetalactones and phenolic acids in methanol extracts of selected Nepeta species, and to evaluate its potential application in chemotaxonomic studies. Material and methods - A UHPLC combined with linear-trap quadrupole (LTQ) orbitrap MS method was used to characterise chemical diversity and complexity of phenolics among 12 selected Nepeta species. A targeted metabolomic approach using UHPLC coupled to a diode array detector (DAD) and combined with (+/-) heated electrospray ionisation (HESI) MS/MS was developed and validated for quantitative analysis of six hydroxycinnamic acid derivatives and four nepetalactones. Results - Phenolic profiling provided a valuable database of bioactive compounds in the plant group studied, including phenolic acids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavones, flavonols and flavanones). Principal component analysis and cluster analysis suggested the applicability of 10 targeted compounds as chemomarkers for chemotaxonomic studies. Pearson's correlation analysis revealed significant positive correlations between metabolites involved in different biosynthetic pathways (phenylpropanoid or monoterpenoid). Conclusion - The described targeted metabolomic approach proved to be highly beneficial in designing a phytochemical overview of the genus Nepeta, and might have applications in further clarification of phylogenetic relations. Furthermore, it has the potential to be implemented in a routine quality control of plant material and herbal preparations.
PB  - Wiley-Blackwell, Hoboken
T2  - Phytochemical Analysis
T1  - Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies
VL  - 26
IS  - 1
SP  - 72
EP  - 85
DO  - 10.1002/pca.2538
ER  - 
@article{
author = "Mišić, Danijela and Šiler, Branislav and Gašić, Uroš M. and Avramov, Stevan N. and Živković, Suzana and Nestorović Živković, Jasmina and Milutinovic, Milica and Tešić, Živoslav Lj.",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1663",
abstract = "Introduction - Nepeta species contain a variety of secondary metabolites, including iridoid monoterpenes - nepetalactones and phenolic acids - that are considered the main bioactive constituents. This work represents the first attempt to comparatively explore variations in these two major groups of secondary metabolites within the genus. Objective - To develop an efficient analytical methodology for simultaneous analysis of nepetalactones and phenolic acids in methanol extracts of selected Nepeta species, and to evaluate its potential application in chemotaxonomic studies. Material and methods - A UHPLC combined with linear-trap quadrupole (LTQ) orbitrap MS method was used to characterise chemical diversity and complexity of phenolics among 12 selected Nepeta species. A targeted metabolomic approach using UHPLC coupled to a diode array detector (DAD) and combined with (+/-) heated electrospray ionisation (HESI) MS/MS was developed and validated for quantitative analysis of six hydroxycinnamic acid derivatives and four nepetalactones. Results - Phenolic profiling provided a valuable database of bioactive compounds in the plant group studied, including phenolic acids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavones, flavonols and flavanones). Principal component analysis and cluster analysis suggested the applicability of 10 targeted compounds as chemomarkers for chemotaxonomic studies. Pearson's correlation analysis revealed significant positive correlations between metabolites involved in different biosynthetic pathways (phenylpropanoid or monoterpenoid). Conclusion - The described targeted metabolomic approach proved to be highly beneficial in designing a phytochemical overview of the genus Nepeta, and might have applications in further clarification of phylogenetic relations. Furthermore, it has the potential to be implemented in a routine quality control of plant material and herbal preparations.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytochemical Analysis",
title = "Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies",
volume = "26",
number = "1",
pages = "72-85",
doi = "10.1002/pca.2538"
}
Mišić, D., Šiler, B., Gašić, U. M., Avramov, S. N., Živković, S., Nestorović Živković, J., Milutinovic, M.,& Tešić, Ž. Lj. (2015). Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies.
Phytochemical Analysis
Wiley-Blackwell, Hoboken., 26(1), 72-85.
https://doi.org/10.1002/pca.2538
Mišić D, Šiler B, Gašić UM, Avramov SN, Živković S, Nestorović Živković J, Milutinovic M, Tešić ŽL. Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis. 2015;26(1):72-85
Mišić Danijela, Šiler Branislav, Gašić Uroš M., Avramov Stevan N., Živković Suzana, Nestorović Živković Jasmina, Milutinovic Milica, Tešić Živoslav Lj., "Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies" Phytochemical Analysis, 26, no. 1 (2015):72-85,
https://doi.org/10.1002/pca.2538 .
50
46
50

Chemical markers for the authentication of unifloral Salvia officinalis L. honey

Gašić, Uroš M.; Natić, Maja; Mišić, Danijela; Lušić, Darija V.; Milojković-Opsenica, Dušanka; Tešić, Živoslav Lj.; Lušić, Dražen

(San Diego : Elsevier Academic Press, 2015)

TY  - JOUR
AU  - Gašić, Uroš M.
AU  - Natić, Maja
AU  - Mišić, Danijela
AU  - Lušić, Darija V.
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav Lj.
AU  - Lušić, Dražen
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1983
AB  - The objective of the present study was to trace phytochemicals that characterize unifloral Common sage (Salvia officinalis L.) honey originating from the Croatian North Adriatic coast. The polyphenolic profiles and total phenolic contents (TPC), the compositions of minerals, sugars and sugar alcohols, and the radical scavenging activities (RSA) of 18 unifloral S. officinalis honey samples were investigated. The quantitative data on the targeted compounds (25 phenolic compounds, 14 carbohydrates and 25 minerals) together with the TPC and RSA data served as a pool of variables for multivariate analysis, which provided useful information for the accurate authentication of unifloral sage honey and its discrimination from other unifloral types of honey. The proposed markers, together with chemometrics, could further contribute, as a powerful tool, to the quality control of Croatian unifloral S. officinalis honey and thus, possibly certify its commercial value.
PB  - San Diego : Elsevier Academic Press
T2  - Journal of Food Composition and Analysis
T1  - Chemical markers for the authentication of unifloral Salvia officinalis L. honey
VL  - 44
SP  - 128
EP  - 138
DO  - 10.1016/j.jfca.2015.08.008
ER  - 
@article{
author = "Gašić, Uroš M. and Natić, Maja and Mišić, Danijela and Lušić, Darija V. and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj. and Lušić, Dražen",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1983",
abstract = "The objective of the present study was to trace phytochemicals that characterize unifloral Common sage (Salvia officinalis L.) honey originating from the Croatian North Adriatic coast. The polyphenolic profiles and total phenolic contents (TPC), the compositions of minerals, sugars and sugar alcohols, and the radical scavenging activities (RSA) of 18 unifloral S. officinalis honey samples were investigated. The quantitative data on the targeted compounds (25 phenolic compounds, 14 carbohydrates and 25 minerals) together with the TPC and RSA data served as a pool of variables for multivariate analysis, which provided useful information for the accurate authentication of unifloral sage honey and its discrimination from other unifloral types of honey. The proposed markers, together with chemometrics, could further contribute, as a powerful tool, to the quality control of Croatian unifloral S. officinalis honey and thus, possibly certify its commercial value.",
publisher = "San Diego : Elsevier Academic Press",
journal = "Journal of Food Composition and Analysis",
title = "Chemical markers for the authentication of unifloral Salvia officinalis L. honey",
volume = "44",
pages = "128-138",
doi = "10.1016/j.jfca.2015.08.008"
}
Gašić, U. M., Natić, M., Mišić, D., Lušić, D. V., Milojković-Opsenica, D., Tešić, Ž. Lj.,& Lušić, D. (2015). Chemical markers for the authentication of unifloral Salvia officinalis L. honey.
Journal of Food Composition and Analysis
San Diego : Elsevier Academic Press., 44, 128-138.
https://doi.org/10.1016/j.jfca.2015.08.008
Gašić UM, Natić M, Mišić D, Lušić DV, Milojković-Opsenica D, Tešić ŽL, Lušić D. Chemical markers for the authentication of unifloral Salvia officinalis L. honey. Journal of Food Composition and Analysis. 2015;44:128-138
Gašić Uroš M., Natić Maja, Mišić Danijela, Lušić Darija V., Milojković-Opsenica Dušanka, Tešić Živoslav Lj., Lušić Dražen, "Chemical markers for the authentication of unifloral Salvia officinalis L. honey" Journal of Food Composition and Analysis, 44 (2015):128-138,
https://doi.org/10.1016/j.jfca.2015.08.008 .
39
34
42

Chemical markers for the authentication of unifloral Salvia officinalis L. honey

Gašić, Uroš M.; Natić, Maja; Mišić, Danijela; Lušić, Darija V.; Milojković-Opsenica, Dušanka; Tešić, Živoslav Lj.; Lušić, Dražen

(San Diego : Elsevier Academic Press, 2015)

TY  - JOUR
AU  - Gašić, Uroš M.
AU  - Natić, Maja
AU  - Mišić, Danijela
AU  - Lušić, Darija V.
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav Lj.
AU  - Lušić, Dražen
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3352
AB  - The objective of the present study was to trace phytochemicals that characterize unifloral Common sage (Salvia officinalis L.) honey originating from the Croatian North Adriatic coast. The polyphenolic profiles and total phenolic contents (TPC), the compositions of minerals, sugars and sugar alcohols, and the radical scavenging activities (RSA) of 18 unifloral S. officinalis honey samples were investigated. The quantitative data on the targeted compounds (25 phenolic compounds, 14 carbohydrates and 25 minerals) together with the TPC and RSA data served as a pool of variables for multivariate analysis, which provided useful information for the accurate authentication of unifloral sage honey and its discrimination from other unifloral types of honey. The proposed markers, together with chemometrics, could further contribute, as a powerful tool, to the quality control of Croatian unifloral S. officinalis honey and thus, possibly certify its commercial value.
PB  - San Diego : Elsevier Academic Press
T2  - Journal of Food Composition and Analysis
T1  - Chemical markers for the authentication of unifloral Salvia officinalis L. honey
VL  - 44
SP  - 128
EP  - 138
DO  - 10.1016/j.jfca.2015.08.008
ER  - 
@article{
author = "Gašić, Uroš M. and Natić, Maja and Mišić, Danijela and Lušić, Darija V. and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj. and Lušić, Dražen",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3352",
abstract = "The objective of the present study was to trace phytochemicals that characterize unifloral Common sage (Salvia officinalis L.) honey originating from the Croatian North Adriatic coast. The polyphenolic profiles and total phenolic contents (TPC), the compositions of minerals, sugars and sugar alcohols, and the radical scavenging activities (RSA) of 18 unifloral S. officinalis honey samples were investigated. The quantitative data on the targeted compounds (25 phenolic compounds, 14 carbohydrates and 25 minerals) together with the TPC and RSA data served as a pool of variables for multivariate analysis, which provided useful information for the accurate authentication of unifloral sage honey and its discrimination from other unifloral types of honey. The proposed markers, together with chemometrics, could further contribute, as a powerful tool, to the quality control of Croatian unifloral S. officinalis honey and thus, possibly certify its commercial value.",
publisher = "San Diego : Elsevier Academic Press",
journal = "Journal of Food Composition and Analysis",
title = "Chemical markers for the authentication of unifloral Salvia officinalis L. honey",
volume = "44",
pages = "128-138",
doi = "10.1016/j.jfca.2015.08.008"
}
Gašić, U. M., Natić, M., Mišić, D., Lušić, D. V., Milojković-Opsenica, D., Tešić, Ž. Lj.,& Lušić, D. (2015). Chemical markers for the authentication of unifloral Salvia officinalis L. honey.
Journal of Food Composition and Analysis
San Diego : Elsevier Academic Press., 44, 128-138.
https://doi.org/10.1016/j.jfca.2015.08.008
Gašić UM, Natić M, Mišić D, Lušić DV, Milojković-Opsenica D, Tešić ŽL, Lušić D. Chemical markers for the authentication of unifloral Salvia officinalis L. honey. Journal of Food Composition and Analysis. 2015;44:128-138
Gašić Uroš M., Natić Maja, Mišić Danijela, Lušić Darija V., Milojković-Opsenica Dušanka, Tešić Živoslav Lj., Lušić Dražen, "Chemical markers for the authentication of unifloral Salvia officinalis L. honey" Journal of Food Composition and Analysis, 44 (2015):128-138,
https://doi.org/10.1016/j.jfca.2015.08.008 .
39
34
42

Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008

Gašić, Uroš M.; Natić, Maja; Mišić, Danijela; Lušić, Darija V.; Milojković-Opsenica, Dušanka; Tešić, Živoslav Lj.; Lušić, Dražen

(San Diego : Elsevier Academic Press, 2015)

TY  - BOOK
AU  - Gašić, Uroš M.
AU  - Natić, Maja
AU  - Mišić, Danijela
AU  - Lušić, Darija V.
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav Lj.
AU  - Lušić, Dražen
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3353
PB  - San Diego : Elsevier Academic Press
T2  - Journal of Food Composition and Analysis
T1  - Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008
ER  - 
@book{
author = "Gašić, Uroš M. and Natić, Maja and Mišić, Danijela and Lušić, Darija V. and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj. and Lušić, Dražen",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3353",
publisher = "San Diego : Elsevier Academic Press",
journal = "Journal of Food Composition and Analysis",
title = "Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008"
}
Gašić, U. M., Natić, M., Mišić, D., Lušić, D. V., Milojković-Opsenica, D., Tešić, Ž. Lj.,& Lušić, D. (2015). Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008.
Journal of Food Composition and Analysis
San Diego : Elsevier Academic Press..
Gašić UM, Natić M, Mišić D, Lušić DV, Milojković-Opsenica D, Tešić ŽL, Lušić D. Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008. Journal of Food Composition and Analysis. 2015;
Gašić Uroš M., Natić Maja, Mišić Danijela, Lušić Darija V., Milojković-Opsenica Dušanka, Tešić Živoslav Lj., Lušić Dražen, "Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008" Journal of Food Composition and Analysis (2015)