Karolinska Institutet

Link to this page

Karolinska Institutet

Authors

Publications

The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1

Apostolović, Danijela; Sanchez-Vidaurre, Sara; Waden, Konrad; Curin, Mirela; Grundström, Jeanette; Gafvelin, Guro; Ćirković-Veličković, Tanja; Gronlund, Hans; Thomas, Wayne R.; Valenta, Rudolf; Hamsten, Carl; van Hage, Marianne

(Wiley-Blackwell, Hoboken, 2016)

TY  - JOUR
AU  - Apostolović, Danijela
AU  - Sanchez-Vidaurre, Sara
AU  - Waden, Konrad
AU  - Curin, Mirela
AU  - Grundström, Jeanette
AU  - Gafvelin, Guro
AU  - Ćirković-Veličković, Tanja
AU  - Gronlund, Hans
AU  - Thomas, Wayne R.
AU  - Valenta, Rudolf
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3650
AB  - We investigated the prevalence of sensitization to the cat lipocalin Fel d 7 among 140 cat-sensitized Swedish patients and elucidated its allergenic activity and cross-reactivity with the dog lipocalin Can f 1. Sixty-five of 140 patients had IgE to rFel d 7 whereof 60 also had IgE to rCan f 1. A moderate correlation between IgE levels to rFel d 7 and rCan f 1 was found. rFel d 7 activated basophils in vitro and inhibited IgE binding to rCan f 1 in 4 of 13 patients, whereas rCan f 1 inhibited IgE binding to rFel d 7 in 7 of 13 patients. Fel d 7 and Can f 1 showed high similarities in protein structure and epitopes in common were found using cross-reactive antisera. Fel d 7 is a common allergen in a Swedish cat-sensitized population that cross-reacts with Can f 1, and may contribute to symptoms in cat-but also in dog-allergic patients.
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1
VL  - 71
IS  - 10
SP  - 1490
EP  - 1495
DO  - 10.1111/all.12955
ER  - 
@article{
author = "Apostolović, Danijela and Sanchez-Vidaurre, Sara and Waden, Konrad and Curin, Mirela and Grundström, Jeanette and Gafvelin, Guro and Ćirković-Veličković, Tanja and Gronlund, Hans and Thomas, Wayne R. and Valenta, Rudolf and Hamsten, Carl and van Hage, Marianne",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3650",
abstract = "We investigated the prevalence of sensitization to the cat lipocalin Fel d 7 among 140 cat-sensitized Swedish patients and elucidated its allergenic activity and cross-reactivity with the dog lipocalin Can f 1. Sixty-five of 140 patients had IgE to rFel d 7 whereof 60 also had IgE to rCan f 1. A moderate correlation between IgE levels to rFel d 7 and rCan f 1 was found. rFel d 7 activated basophils in vitro and inhibited IgE binding to rCan f 1 in 4 of 13 patients, whereas rCan f 1 inhibited IgE binding to rFel d 7 in 7 of 13 patients. Fel d 7 and Can f 1 showed high similarities in protein structure and epitopes in common were found using cross-reactive antisera. Fel d 7 is a common allergen in a Swedish cat-sensitized population that cross-reacts with Can f 1, and may contribute to symptoms in cat-but also in dog-allergic patients.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1",
volume = "71",
number = "10",
pages = "1490-1495",
doi = "10.1111/all.12955"
}
Apostolović, D., Sanchez-Vidaurre, S., Waden, K., Curin, M., Grundström, J., Gafvelin, G., Ćirković-Veličković, T., Gronlund, H., Thomas, W. R., Valenta, R., Hamsten, C.,& van Hage, M. (2016). The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1.
Allergy
Wiley-Blackwell, Hoboken., 71(10), 1490-1495.
https://doi.org/10.1111/all.12955
Apostolović D, Sanchez-Vidaurre S, Waden K, Curin M, Grundström J, Gafvelin G, Ćirković-Veličković T, Gronlund H, Thomas WR, Valenta R, Hamsten C, van Hage M. The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1. Allergy. 2016;71(10):1490-1495
Apostolović Danijela, Sanchez-Vidaurre Sara, Waden Konrad, Curin Mirela, Grundström Jeanette, Gafvelin Guro, Ćirković-Veličković Tanja, Gronlund Hans, Thomas Wayne R., Valenta Rudolf, Hamsten Carl, van Hage Marianne, "The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1" Allergy, 71, no. 10 (2016):1490-1495,
https://doi.org/10.1111/all.12955 .
62
25
18
19

Supplementary data for the article: Apostolovic, D.; Sánchez-Vidaurre, S.; Waden, K.; Curin, M.; Grundström, J.; Gafvelin, G.; Cirkovic Velickovic, T.; Grönlund, H.; Thomas, W. R.; Valenta, R.; et al. The Cat Lipocalin Fel d 7 and Its Cross-Reactivity with the Dog Lipocalin Can f 1. Allergy: European Journal of Allergy and Clinical Immunology 2016, 71 (10), 1490–1495. https://doi.org/10.1111/all.12955

Apostolović, Danijela; Sanchez-Vidaurre, Sara; Waden, Konrad; Curin, Mirela; Grundström, Jeanette; Gafvelin, Guro; Ćirković-Veličković, Tanja; Gronlund, Hans; Thomas, Wayne R.; Valenta, Rudolf; Hamsten, Carl; van Hage, Marianne

(Wiley-Blackwell, Hoboken, 2016)

TY  - BOOK
AU  - Apostolović, Danijela
AU  - Sanchez-Vidaurre, Sara
AU  - Waden, Konrad
AU  - Curin, Mirela
AU  - Grundström, Jeanette
AU  - Gafvelin, Guro
AU  - Ćirković-Veličković, Tanja
AU  - Gronlund, Hans
AU  - Thomas, Wayne R.
AU  - Valenta, Rudolf
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3651
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - Supplementary data for the article: Apostolovic, D.; Sánchez-Vidaurre, S.; Waden, K.; Curin, M.; Grundström, J.; Gafvelin, G.; Cirkovic Velickovic, T.; Grönlund, H.; Thomas, W. R.; Valenta, R.; et al. The Cat Lipocalin Fel d 7 and Its Cross-Reactivity with the Dog Lipocalin Can f 1. Allergy: European Journal of Allergy and Clinical Immunology 2016, 71 (10), 1490–1495. https://doi.org/10.1111/all.12955
ER  - 
@book{
author = "Apostolović, Danijela and Sanchez-Vidaurre, Sara and Waden, Konrad and Curin, Mirela and Grundström, Jeanette and Gafvelin, Guro and Ćirković-Veličković, Tanja and Gronlund, Hans and Thomas, Wayne R. and Valenta, Rudolf and Hamsten, Carl and van Hage, Marianne",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3651",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Supplementary data for the article: Apostolovic, D.; Sánchez-Vidaurre, S.; Waden, K.; Curin, M.; Grundström, J.; Gafvelin, G.; Cirkovic Velickovic, T.; Grönlund, H.; Thomas, W. R.; Valenta, R.; et al. The Cat Lipocalin Fel d 7 and Its Cross-Reactivity with the Dog Lipocalin Can f 1. Allergy: European Journal of Allergy and Clinical Immunology 2016, 71 (10), 1490–1495. https://doi.org/10.1111/all.12955"
}
Apostolović, D., Sanchez-Vidaurre, S., Waden, K., Curin, M., Grundström, J., Gafvelin, G., Ćirković-Veličković, T., Gronlund, H., Thomas, W. R., Valenta, R., Hamsten, C.,& van Hage, M. (2016). Supplementary data for the article: Apostolovic, D.; Sánchez-Vidaurre, S.; Waden, K.; Curin, M.; Grundström, J.; Gafvelin, G.; Cirkovic Velickovic, T.; Grönlund, H.; Thomas, W. R.; Valenta, R.; et al. The Cat Lipocalin Fel d 7 and Its Cross-Reactivity with the Dog Lipocalin Can f 1. Allergy: European Journal of Allergy and Clinical Immunology 2016, 71 (10), 1490–1495. https://doi.org/10.1111/all.12955.
Allergy
Wiley-Blackwell, Hoboken..
Apostolović D, Sanchez-Vidaurre S, Waden K, Curin M, Grundström J, Gafvelin G, Ćirković-Veličković T, Gronlund H, Thomas WR, Valenta R, Hamsten C, van Hage M. Supplementary data for the article: Apostolovic, D.; Sánchez-Vidaurre, S.; Waden, K.; Curin, M.; Grundström, J.; Gafvelin, G.; Cirkovic Velickovic, T.; Grönlund, H.; Thomas, W. R.; Valenta, R.; et al. The Cat Lipocalin Fel d 7 and Its Cross-Reactivity with the Dog Lipocalin Can f 1. Allergy: European Journal of Allergy and Clinical Immunology 2016, 71 (10), 1490–1495. https://doi.org/10.1111/all.12955. Allergy. 2016;
Apostolović Danijela, Sanchez-Vidaurre Sara, Waden Konrad, Curin Mirela, Grundström Jeanette, Gafvelin Guro, Ćirković-Veličković Tanja, Gronlund Hans, Thomas Wayne R., Valenta Rudolf, Hamsten Carl, van Hage Marianne, "Supplementary data for the article: Apostolovic, D.; Sánchez-Vidaurre, S.; Waden, K.; Curin, M.; Grundström, J.; Gafvelin, G.; Cirkovic Velickovic, T.; Grönlund, H.; Thomas, W. R.; Valenta, R.; et al. The Cat Lipocalin Fel d 7 and Its Cross-Reactivity with the Dog Lipocalin Can f 1. Allergy: European Journal of Allergy and Clinical Immunology 2016, 71 (10), 1490–1495. https://doi.org/10.1111/all.12955" Allergy (2016)

The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1

Apostolović, Danijela; Sanchez-Vidaurre, Sara; Waden, Konrad; Curin, Mirela; Grundström, Jeanette; Gafvelin, Guro; Ćirković-Veličković, Tanja; Gronlund, Hans; Thomas, Wayne R.; Valenta, Rudolf; Hamsten, Carl; van Hage, Marianne

(Wiley-Blackwell, Hoboken, 2016)

TY  - JOUR
AU  - Apostolović, Danijela
AU  - Sanchez-Vidaurre, Sara
AU  - Waden, Konrad
AU  - Curin, Mirela
AU  - Grundström, Jeanette
AU  - Gafvelin, Guro
AU  - Ćirković-Veličković, Tanja
AU  - Gronlund, Hans
AU  - Thomas, Wayne R.
AU  - Valenta, Rudolf
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2337
AB  - We investigated the prevalence of sensitization to the cat lipocalin Fel d 7 among 140 cat-sensitized Swedish patients and elucidated its allergenic activity and cross-reactivity with the dog lipocalin Can f 1. Sixty-five of 140 patients had IgE to rFel d 7 whereof 60 also had IgE to rCan f 1. A moderate correlation between IgE levels to rFel d 7 and rCan f 1 was found. rFel d 7 activated basophils in vitro and inhibited IgE binding to rCan f 1 in 4 of 13 patients, whereas rCan f 1 inhibited IgE binding to rFel d 7 in 7 of 13 patients. Fel d 7 and Can f 1 showed high similarities in protein structure and epitopes in common were found using cross-reactive antisera. Fel d 7 is a common allergen in a Swedish cat-sensitized population that cross-reacts with Can f 1, and may contribute to symptoms in cat-but also in dog-allergic patients.
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1
VL  - 71
IS  - 10
SP  - 1490
EP  - 1495
DO  - 10.1111/all.12955
ER  - 
@article{
author = "Apostolović, Danijela and Sanchez-Vidaurre, Sara and Waden, Konrad and Curin, Mirela and Grundström, Jeanette and Gafvelin, Guro and Ćirković-Veličković, Tanja and Gronlund, Hans and Thomas, Wayne R. and Valenta, Rudolf and Hamsten, Carl and van Hage, Marianne",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2337",
abstract = "We investigated the prevalence of sensitization to the cat lipocalin Fel d 7 among 140 cat-sensitized Swedish patients and elucidated its allergenic activity and cross-reactivity with the dog lipocalin Can f 1. Sixty-five of 140 patients had IgE to rFel d 7 whereof 60 also had IgE to rCan f 1. A moderate correlation between IgE levels to rFel d 7 and rCan f 1 was found. rFel d 7 activated basophils in vitro and inhibited IgE binding to rCan f 1 in 4 of 13 patients, whereas rCan f 1 inhibited IgE binding to rFel d 7 in 7 of 13 patients. Fel d 7 and Can f 1 showed high similarities in protein structure and epitopes in common were found using cross-reactive antisera. Fel d 7 is a common allergen in a Swedish cat-sensitized population that cross-reacts with Can f 1, and may contribute to symptoms in cat-but also in dog-allergic patients.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1",
volume = "71",
number = "10",
pages = "1490-1495",
doi = "10.1111/all.12955"
}
Apostolović, D., Sanchez-Vidaurre, S., Waden, K., Curin, M., Grundström, J., Gafvelin, G., Ćirković-Veličković, T., Gronlund, H., Thomas, W. R., Valenta, R., Hamsten, C.,& van Hage, M. (2016). The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1.
Allergy
Wiley-Blackwell, Hoboken., 71(10), 1490-1495.
https://doi.org/10.1111/all.12955
Apostolović D, Sanchez-Vidaurre S, Waden K, Curin M, Grundström J, Gafvelin G, Ćirković-Veličković T, Gronlund H, Thomas WR, Valenta R, Hamsten C, van Hage M. The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1. Allergy. 2016;71(10):1490-1495
Apostolović Danijela, Sanchez-Vidaurre Sara, Waden Konrad, Curin Mirela, Grundström Jeanette, Gafvelin Guro, Ćirković-Veličković Tanja, Gronlund Hans, Thomas Wayne R., Valenta Rudolf, Hamsten Carl, van Hage Marianne, "The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1" Allergy, 71, no. 10 (2016):1490-1495,
https://doi.org/10.1111/all.12955 .
62
25
18
19

Supplementary data for article: Apostolovic, D.; Tran, T. A. T.; Sánchez-Vidaurre, S.; Cirkovic Velickovic, T.; Starkhammar, M.; Hamsten, C.; Van Hage, M. Red Meat Allergic Patients Have a Selective IgE Response to the α-Gal Glycan. Allergy: European Journal of Allergy and Clinical Immunology 2015, 70 (11), 1497–1500. https://doi.org/10.1111/all.12695

Apostolović, Danijela; Tran, Thi Anh Thu; Sanchez-Vidaurre, Sara; Ćirković-Veličković, Tanja; Starkhammar, Maria; Hamsten, Carl; van Hage, Marianne

(Wiley, Hoboken, 2015)

TY  - BOOK
AU  - Apostolović, Danijela
AU  - Tran, Thi Anh Thu
AU  - Sanchez-Vidaurre, Sara
AU  - Ćirković-Veličković, Tanja
AU  - Starkhammar, Maria
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3407
PB  - Wiley, Hoboken
T2  - Allergy
T1  - Supplementary data for article: Apostolovic, D.; Tran, T. A. T.; Sánchez-Vidaurre, S.; Cirkovic Velickovic, T.; Starkhammar, M.; Hamsten, C.; Van Hage, M. Red Meat Allergic Patients Have a Selective IgE Response to the α-Gal Glycan. Allergy: European Journal of Allergy and Clinical Immunology 2015, 70 (11), 1497–1500. https://doi.org/10.1111/all.12695
ER  - 
@book{
author = "Apostolović, Danijela and Tran, Thi Anh Thu and Sanchez-Vidaurre, Sara and Ćirković-Veličković, Tanja and Starkhammar, Maria and Hamsten, Carl and van Hage, Marianne",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3407",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "Supplementary data for article: Apostolovic, D.; Tran, T. A. T.; Sánchez-Vidaurre, S.; Cirkovic Velickovic, T.; Starkhammar, M.; Hamsten, C.; Van Hage, M. Red Meat Allergic Patients Have a Selective IgE Response to the α-Gal Glycan. Allergy: European Journal of Allergy and Clinical Immunology 2015, 70 (11), 1497–1500. https://doi.org/10.1111/all.12695"
}
Apostolović, D., Tran, T. A. T., Sanchez-Vidaurre, S., Ćirković-Veličković, T., Starkhammar, M., Hamsten, C.,& van Hage, M. (2015). Supplementary data for article: Apostolovic, D.; Tran, T. A. T.; Sánchez-Vidaurre, S.; Cirkovic Velickovic, T.; Starkhammar, M.; Hamsten, C.; Van Hage, M. Red Meat Allergic Patients Have a Selective IgE Response to the α-Gal Glycan. Allergy: European Journal of Allergy and Clinical Immunology 2015, 70 (11), 1497–1500. https://doi.org/10.1111/all.12695.
Allergy
Wiley, Hoboken..
Apostolović D, Tran TAT, Sanchez-Vidaurre S, Ćirković-Veličković T, Starkhammar M, Hamsten C, van Hage M. Supplementary data for article: Apostolovic, D.; Tran, T. A. T.; Sánchez-Vidaurre, S.; Cirkovic Velickovic, T.; Starkhammar, M.; Hamsten, C.; Van Hage, M. Red Meat Allergic Patients Have a Selective IgE Response to the α-Gal Glycan. Allergy: European Journal of Allergy and Clinical Immunology 2015, 70 (11), 1497–1500. https://doi.org/10.1111/all.12695. Allergy. 2015;
Apostolović Danijela, Tran Thi Anh Thu, Sanchez-Vidaurre Sara, Ćirković-Veličković Tanja, Starkhammar Maria, Hamsten Carl, van Hage Marianne, "Supplementary data for article: Apostolovic, D.; Tran, T. A. T.; Sánchez-Vidaurre, S.; Cirkovic Velickovic, T.; Starkhammar, M.; Hamsten, C.; Van Hage, M. Red Meat Allergic Patients Have a Selective IgE Response to the α-Gal Glycan. Allergy: European Journal of Allergy and Clinical Immunology 2015, 70 (11), 1497–1500. https://doi.org/10.1111/all.12695" Allergy (2015)

Red meat allergic patients have a selective IgE response to the alpha-Gal glycan

Apostolović, Danijela; Tran, Thi Anh Thu; Sanchez-Vidaurre, Sara; Ćirković-Veličković, Tanja; Starkhammar, Maria; Hamsten, Carl; van Hage, Marianne

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Apostolović, Danijela
AU  - Tran, Thi Anh Thu
AU  - Sanchez-Vidaurre, Sara
AU  - Ćirković-Veličković, Tanja
AU  - Starkhammar, Maria
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1982
AB  - Galactose--1,3-galactose (-Gal) is a mammalian carbohydrate with significance in a novel type of food allergy. Patients with IgE against -Gal report severe allergic symptoms 3-6h after consumption of red meat. We investigated whether IgE from red meat allergic patients recognizes other mammalian glycans than -Gal or glycans from the plant kingdom and insects of importance in allergy. We found that none of the 24 red meat allergic patients investigated had an IgE antibody response against the other abundant mammalian glycan N-glycolylneuraminic acid or against cross-reactive carbohydrate determinants from plant or venom sources (nCup a 1, nArt v 1, and MUXF3). Deglycosylation of an -Gal-containing protein, bovine thyroglobulin, significantly reduced the IgE response. In conclusion, we show that red meat allergic patients have a selective IgE response to the -Gal glycan found in red meat. Other common glycans reactive in allergic disease are not targets of red meat allergic patients' IgE.
PB  - Wiley, Hoboken
T2  - Allergy
T1  - Red meat allergic patients have a selective IgE response to the alpha-Gal glycan
VL  - 70
IS  - 11
SP  - 1497
EP  - 1500
DO  - 10.1111/all.12695
ER  - 
@article{
author = "Apostolović, Danijela and Tran, Thi Anh Thu and Sanchez-Vidaurre, Sara and Ćirković-Veličković, Tanja and Starkhammar, Maria and Hamsten, Carl and van Hage, Marianne",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1982",
abstract = "Galactose--1,3-galactose (-Gal) is a mammalian carbohydrate with significance in a novel type of food allergy. Patients with IgE against -Gal report severe allergic symptoms 3-6h after consumption of red meat. We investigated whether IgE from red meat allergic patients recognizes other mammalian glycans than -Gal or glycans from the plant kingdom and insects of importance in allergy. We found that none of the 24 red meat allergic patients investigated had an IgE antibody response against the other abundant mammalian glycan N-glycolylneuraminic acid or against cross-reactive carbohydrate determinants from plant or venom sources (nCup a 1, nArt v 1, and MUXF3). Deglycosylation of an -Gal-containing protein, bovine thyroglobulin, significantly reduced the IgE response. In conclusion, we show that red meat allergic patients have a selective IgE response to the -Gal glycan found in red meat. Other common glycans reactive in allergic disease are not targets of red meat allergic patients' IgE.",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "Red meat allergic patients have a selective IgE response to the alpha-Gal glycan",
volume = "70",
number = "11",
pages = "1497-1500",
doi = "10.1111/all.12695"
}
Apostolović, D., Tran, T. A. T., Sanchez-Vidaurre, S., Ćirković-Veličković, T., Starkhammar, M., Hamsten, C.,& van Hage, M. (2015). Red meat allergic patients have a selective IgE response to the alpha-Gal glycan.
Allergy
Wiley, Hoboken., 70(11), 1497-1500.
https://doi.org/10.1111/all.12695
Apostolović D, Tran TAT, Sanchez-Vidaurre S, Ćirković-Veličković T, Starkhammar M, Hamsten C, van Hage M. Red meat allergic patients have a selective IgE response to the alpha-Gal glycan. Allergy. 2015;70(11):1497-1500
Apostolović Danijela, Tran Thi Anh Thu, Sanchez-Vidaurre Sara, Ćirković-Veličković Tanja, Starkhammar Maria, Hamsten Carl, van Hage Marianne, "Red meat allergic patients have a selective IgE response to the alpha-Gal glycan" Allergy, 70, no. 11 (2015):1497-1500,
https://doi.org/10.1111/all.12695 .
29
18
15
20

Immunoproteomics of processed beef proteins reveal novel galactose-alpha-1,3-galactose-containing allergens

Apostolović, Danijela; Tran, Thi Anh Thu; Hamsten, Carl; Starkhammar, Maria; Ćirković-Veličković, Tanja; van Hage, Marianne

(Wiley, Hoboken, 2014)

TY  - JOUR
AU  - Apostolović, Danijela
AU  - Tran, Thi Anh Thu
AU  - Hamsten, Carl
AU  - Starkhammar, Maria
AU  - Ćirković-Veličković, Tanja
AU  - van Hage, Marianne
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1858
AB  - BackgroundRed meat allergy presents a novel form of food allergy with severe delayed allergic reactions where IgE antibodies are directed against the carbohydrate -Gal epitope. Food preparation and processing can influence the allergenicity of proteins. The aim of this study was to characterize the proteomic profile of different beef preparations and to investigate their -Gal reactivity and potential allergenicity. MethodsExtracts from raw, boiled, fried, and medium rare prepared beef were assessed by 2D PAGE for the comparison of protein profiles. IgE-binding proteins were identified using immunoblot-coupled proteomic analysis using sera from red meat-allergic patients. Presence of the -Gal epitope was verified using anti--Gal antibody and IgE inhibition immunoblot with -Gal. ResultsMultiple IgE-binding proteins were detected in the different beef preparations, many of which were also recognized by the anti--Gal antibody. Protein spots reacting with IgE in patient sera were analyzed by MS/MS, resulting in identification of 18 proteins with high identification scores. Seven of the 18 beef allergens identified using meat-allergic patient sera were also recognized by the anti--Gal monoclonal antibody, and four of them were stabile to thermal treatment. Furthermore, a dose-dependent inhibition of red meat-allergic patients' IgE to beef by -Gal was demonstrated. ConclusionsWe show that the -Gal epitope is commonly present in IgE-reactive beef proteins recognized by meat-allergic patients. Seven novel -Gal-containing IgE-binding proteins were identified, of which four were stable to heat treatment. Thus, the allergenicity of red meat proteins is preserved even upon different thermal cooking.
PB  - Wiley, Hoboken
T2  - Allergy
T1  - Immunoproteomics of processed beef proteins reveal novel galactose-alpha-1,3-galactose-containing allergens
VL  - 69
IS  - 10
SP  - 1308
EP  - 1315
DO  - 10.1111/all.12462
ER  - 
@article{
author = "Apostolović, Danijela and Tran, Thi Anh Thu and Hamsten, Carl and Starkhammar, Maria and Ćirković-Veličković, Tanja and van Hage, Marianne",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1858",
abstract = "BackgroundRed meat allergy presents a novel form of food allergy with severe delayed allergic reactions where IgE antibodies are directed against the carbohydrate -Gal epitope. Food preparation and processing can influence the allergenicity of proteins. The aim of this study was to characterize the proteomic profile of different beef preparations and to investigate their -Gal reactivity and potential allergenicity. MethodsExtracts from raw, boiled, fried, and medium rare prepared beef were assessed by 2D PAGE for the comparison of protein profiles. IgE-binding proteins were identified using immunoblot-coupled proteomic analysis using sera from red meat-allergic patients. Presence of the -Gal epitope was verified using anti--Gal antibody and IgE inhibition immunoblot with -Gal. ResultsMultiple IgE-binding proteins were detected in the different beef preparations, many of which were also recognized by the anti--Gal antibody. Protein spots reacting with IgE in patient sera were analyzed by MS/MS, resulting in identification of 18 proteins with high identification scores. Seven of the 18 beef allergens identified using meat-allergic patient sera were also recognized by the anti--Gal monoclonal antibody, and four of them were stabile to thermal treatment. Furthermore, a dose-dependent inhibition of red meat-allergic patients' IgE to beef by -Gal was demonstrated. ConclusionsWe show that the -Gal epitope is commonly present in IgE-reactive beef proteins recognized by meat-allergic patients. Seven novel -Gal-containing IgE-binding proteins were identified, of which four were stable to heat treatment. Thus, the allergenicity of red meat proteins is preserved even upon different thermal cooking.",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "Immunoproteomics of processed beef proteins reveal novel galactose-alpha-1,3-galactose-containing allergens",
volume = "69",
number = "10",
pages = "1308-1315",
doi = "10.1111/all.12462"
}
Apostolović, D., Tran, T. A. T., Hamsten, C., Starkhammar, M., Ćirković-Veličković, T.,& van Hage, M. (2014). Immunoproteomics of processed beef proteins reveal novel galactose-alpha-1,3-galactose-containing allergens.
Allergy
Wiley, Hoboken., 69(10), 1308-1315.
https://doi.org/10.1111/all.12462
Apostolović D, Tran TAT, Hamsten C, Starkhammar M, Ćirković-Veličković T, van Hage M. Immunoproteomics of processed beef proteins reveal novel galactose-alpha-1,3-galactose-containing allergens. Allergy. 2014;69(10):1308-1315
Apostolović Danijela, Tran Thi Anh Thu, Hamsten Carl, Starkhammar Maria, Ćirković-Veličković Tanja, van Hage Marianne, "Immunoproteomics of processed beef proteins reveal novel galactose-alpha-1,3-galactose-containing allergens" Allergy, 69, no. 10 (2014):1308-1315,
https://doi.org/10.1111/all.12462 .
1
40
34
39

Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130

Polović, Natalija; Waden, Konrad; Binnmyr, J.; Hamsten, Carl; Gronneberg, R.; Palmberg, C.; Milčić-Matić, Natalija; Bergman, T.; Gronlund, Hans; van Hage, Marianne

(Wiley-Blackwell, Hoboken, 2013)

TY  - BOOK
AU  - Polović, Natalija
AU  - Waden, Konrad
AU  - Binnmyr, J.
AU  - Hamsten, Carl
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, Hans
AU  - van Hage, Marianne
PY  - 2013
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3488
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130
ER  - 
@book{
author = "Polović, Natalija and Waden, Konrad and Binnmyr, J. and Hamsten, Carl and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, Hans and van Hage, Marianne",
year = "2013",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3488",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130"
}
Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M. (2013). Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130.
Allergy
Wiley-Blackwell, Hoboken..
Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130. Allergy. 2013;
Polović Natalija, Waden Konrad, Binnmyr J., Hamsten Carl, Gronneberg R., Palmberg C., Milčić-Matić Natalija, Bergman T., Gronlund Hans, van Hage Marianne, "Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130" Allergy (2013)

Dog saliva - an important source of dog allergens

Polović, Natalija; Waden, Konrad; Binnmyr, J.; Hamsten, Carl; Gronneberg, R.; Palmberg, C.; Milčić-Matić, Natalija; Bergman, T.; Gronlund, Hans; van Hage, Marianne

(Wiley-Blackwell, Hoboken, 2013)

TY  - JOUR
AU  - Polović, Natalija
AU  - Waden, Konrad
AU  - Binnmyr, J.
AU  - Hamsten, Carl
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, Hans
AU  - van Hage, Marianne
PY  - 2013
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1634
AB  - Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - Dog saliva - an important source of dog allergens
VL  - 68
IS  - 5
SP  - 585
EP  - 592
DO  - 10.1111/all.12130
ER  - 
@article{
author = "Polović, Natalija and Waden, Konrad and Binnmyr, J. and Hamsten, Carl and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, Hans and van Hage, Marianne",
year = "2013",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1634",
abstract = "Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Dog saliva - an important source of dog allergens",
volume = "68",
number = "5",
pages = "585-592",
doi = "10.1111/all.12130"
}
Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M. (2013). Dog saliva - an important source of dog allergens.
Allergy
Wiley-Blackwell, Hoboken., 68(5), 585-592.
https://doi.org/10.1111/all.12130
Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Dog saliva - an important source of dog allergens. Allergy. 2013;68(5):585-592
Polović Natalija, Waden Konrad, Binnmyr J., Hamsten Carl, Gronneberg R., Palmberg C., Milčić-Matić Natalija, Bergman T., Gronlund Hans, van Hage Marianne, "Dog saliva - an important source of dog allergens" Allergy, 68, no. 5 (2013):585-592,
https://doi.org/10.1111/all.12130 .
12
29
23
24