TY  - JOUR
AU  - Lončar, Nikola L.
AU  - Šokarda-Slavić, Marinela
AU  - Vujčić, Zoran
AU  - Božić, Nataša
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1985
AB  - Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases
VL  - 5
DO  - 10.1038/srep15772
ER  - 

@article{
author = "Lončar, Nikola L. and Šokarda-Slavić, Marinela and Vujčić, Zoran and Božić, Nataša",
year = "2015",
abstract = "Bacillus licheniformis 9945a alpha-amylase is known as a potent enzyme for raw starch hydrolysis. In this paper, a mixed mode Nuvia cPrime (TM) resin is examined with the aim to improve the downstream processing of raw starch digesting amylases and exploit the hydrophobic patches on their surface. This resin combines hydrophobic interactions with cation exchange groups and as such the presence of salt facilitates hydrophobic interactions while the ion-exchange groups enable proper selectivity. alpha-Amylase was produced using an optimized fed-batch approach in a defined media and significant overexpression of 1.2 g L-1 was achieved. This single step procedure enables simultaneous concentration, pigment removal as well as purification of amylase with yields of 96% directly from the fermentation broth.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases",
volume = "5",
doi = "10.1038/srep15772"
}

Lončar, N. L., Šokarda-Slavić, M., Vujčić, Z.,& Božić, N.. (2015). Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases. in Scientific Reports
Nature Publishing Group, London., 5.
https://doi.org/10.1038/srep15772

Lončar NL, Šokarda-Slavić M, Vujčić Z, Božić N. Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases. in Scientific Reports. 2015;5.
doi:10.1038/srep15772 .

Lončar, Nikola L., Šokarda-Slavić, Marinela, Vujčić, Zoran, Božić, Nataša, "Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting alpha-amylases" in Scientific Reports, 5 (2015),
https://doi.org/10.1038/srep15772 . .

TY  - DATA
AU  - Lončar, Nikola L.
AU  - Šokarda-Slavić, Marinela
AU  - Vujčić, Zoran
AU  - Božić, Nataša
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3384
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Supplementary data for article: Lončar, N.; Slavić, M. Š.; Vujčić, Z.; Božić, N. Mixed-Mode Resins: Taking Shortcut in Downstream Processing of Raw-Starch Digesting α-Amylases. Scientific Reports 2015, 5. https://doi.org/10.1038/srep15772
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3384
ER  - 

@misc{
author = "Lončar, Nikola L. and Šokarda-Slavić, Marinela and Vujčić, Zoran and Božić, Nataša",
year = "2015",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Supplementary data for article: Lončar, N.; Slavić, M. Š.; Vujčić, Z.; Božić, N. Mixed-Mode Resins: Taking Shortcut in Downstream Processing of Raw-Starch Digesting α-Amylases. Scientific Reports 2015, 5. https://doi.org/10.1038/srep15772",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3384"
}

Lončar, N. L., Šokarda-Slavić, M., Vujčić, Z.,& Božić, N.. (2015). Supplementary data for article: Lončar, N.; Slavić, M. Š.; Vujčić, Z.; Božić, N. Mixed-Mode Resins: Taking Shortcut in Downstream Processing of Raw-Starch Digesting α-Amylases. Scientific Reports 2015, 5. https://doi.org/10.1038/srep15772. in Scientific Reports
Nature Publishing Group, London..
https://hdl.handle.net/21.15107/rcub_cherry_3384

Lončar NL, Šokarda-Slavić M, Vujčić Z, Božić N. Supplementary data for article: Lončar, N.; Slavić, M. Š.; Vujčić, Z.; Božić, N. Mixed-Mode Resins: Taking Shortcut in Downstream Processing of Raw-Starch Digesting α-Amylases. Scientific Reports 2015, 5. https://doi.org/10.1038/srep15772. in Scientific Reports. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3384 .

Lončar, Nikola L., Šokarda-Slavić, Marinela, Vujčić, Zoran, Božić, Nataša, "Supplementary data for article: Lončar, N.; Slavić, M. Š.; Vujčić, Z.; Božić, N. Mixed-Mode Resins: Taking Shortcut in Downstream Processing of Raw-Starch Digesting α-Amylases. Scientific Reports 2015, 5. https://doi.org/10.1038/srep15772" in Scientific Reports (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3384 .