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United States Department of Agriculture under Agricultural Research Service [5348-32000-033-00D]

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alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells

Krstić-Ristivojević, Maja; Grundström, Jeanette; Tran, Thi Anh Thu; Apostolović, Danijela; Radoi, Vlad; Starkhammar, Maria; Vukojević, Vesna; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Nature Publishing Group, London, 2018)

TY  - JOUR
AU  - Krstić-Ristivojević, Maja
AU  - Grundström, Jeanette
AU  - Tran, Thi Anh Thu
AU  - Apostolović, Danijela
AU  - Radoi, Vlad
AU  - Starkhammar, Maria
AU  - Vukojević, Vesna
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2207
AB  - Red meat allergy is characterized by an IgE response against the carbohydrate galactose-alpha-1,3-galactose (alpha-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how alpha-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of alpha-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of alpha-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of alpha-Gal carrying proteins was significantly higher than the uptake of non-alpha-Gal carrying proteins. Confocal microscopy revealed alpha-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying alpha-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of alpha-Gal carrying proteins was slower than degradation of non-alpha-Gal carrying proteins. Thus, the presence of alpha-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of alpha-Gal as a clinically relevant food allergen.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells
VL  - 8
DO  - 10.1038/s41598-018-30887-8
UR  - Kon_3538
ER  - 
@article{
author = "Krstić-Ristivojević, Maja and Grundström, Jeanette and Tran, Thi Anh Thu and Apostolović, Danijela and Radoi, Vlad and Starkhammar, Maria and Vukojević, Vesna and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2018",
abstract = "Red meat allergy is characterized by an IgE response against the carbohydrate galactose-alpha-1,3-galactose (alpha-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how alpha-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of alpha-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of alpha-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of alpha-Gal carrying proteins was significantly higher than the uptake of non-alpha-Gal carrying proteins. Confocal microscopy revealed alpha-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying alpha-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of alpha-Gal carrying proteins was slower than degradation of non-alpha-Gal carrying proteins. Thus, the presence of alpha-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of alpha-Gal as a clinically relevant food allergen.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells",
volume = "8",
doi = "10.1038/s41598-018-30887-8",
url = "Kon_3538"
}
Krstić-Ristivojević, M., Grundström, J., Tran, T. A. T., Apostolović, D., Radoi, V., Starkhammar, M., Vukojević, V., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M.. (2018). alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells. in Scientific Reports
Nature Publishing Group, London., 8.
https://doi.org/10.1038/s41598-018-30887-8
Kon_3538
Krstić-Ristivojević M, Grundström J, Tran TAT, Apostolović D, Radoi V, Starkhammar M, Vukojević V, Ćirković-Veličković T, Hamsten C, van Hage M. alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells. in Scientific Reports. 2018;8.
doi:10.1038/s41598-018-30887-8
Kon_3538 .
Krstić-Ristivojević, Maja, Grundström, Jeanette, Tran, Thi Anh Thu, Apostolović, Danijela, Radoi, Vlad, Starkhammar, Maria, Vukojević, Vesna, Ćirković-Veličković, Tanja, Hamsten, Carl, van Hage, Marianne, "alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells" in Scientific Reports, 8 (2018),
https://doi.org/10.1038/s41598-018-30887-8 .,
Kon_3538 .
4
7
5
6

Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8

Krstić-Ristivojević, Maja; Grundström, Jeanette; Tran, Thi Anh Thu; Apostolović, Danijela; Radoi, Vlad; Starkhammar, Maria; Vukojević, Vesna; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Nature Publishing Group, London, 2018)

TY  - DATA
AU  - Krstić-Ristivojević, Maja
AU  - Grundström, Jeanette
AU  - Tran, Thi Anh Thu
AU  - Apostolović, Danijela
AU  - Radoi, Vlad
AU  - Starkhammar, Maria
AU  - Vukojević, Vesna
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3073
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8
UR  - Kon_3538
ER  - 
@misc{
author = "Krstić-Ristivojević, Maja and Grundström, Jeanette and Tran, Thi Anh Thu and Apostolović, Danijela and Radoi, Vlad and Starkhammar, Maria and Vukojević, Vesna and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2018",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8",
url = "Kon_3538"
}
Krstić-Ristivojević, M., Grundström, J., Tran, T. A. T., Apostolović, D., Radoi, V., Starkhammar, M., Vukojević, V., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M.. (2018). Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8. in Scientific Reports
Nature Publishing Group, London..
Kon_3538
Krstić-Ristivojević M, Grundström J, Tran TAT, Apostolović D, Radoi V, Starkhammar M, Vukojević V, Ćirković-Veličković T, Hamsten C, van Hage M. Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8. in Scientific Reports. 2018;.
Kon_3538 .
Krstić-Ristivojević, Maja, Grundström, Jeanette, Tran, Thi Anh Thu, Apostolović, Danijela, Radoi, Vlad, Starkhammar, Maria, Vukojević, Vesna, Ćirković-Veličković, Tanja, Hamsten, Carl, van Hage, Marianne, "Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8" in Scientific Reports (2018),
Kon_3538 .