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Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e

Chen, Qiushui; Utech, Stefanie; Chen, Dong; Prodanović, Radivoje; Lin, Jin-Ming; Weitz, David A.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - DATA
AU  - Chen, Qiushui
AU  - Utech, Stefanie
AU  - Chen, Dong
AU  - Prodanović, Radivoje
AU  - Lin, Jin-Ming
AU  - Weitz, David A.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3427
PB  - Royal Soc Chemistry, Cambridge
T2  - Lab on A Chip
T1  - Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e
ER  - 
@misc{
author = "Chen, Qiushui and Utech, Stefanie and Chen, Dong and Prodanović, Radivoje and Lin, Jin-Ming and Weitz, David A.",
year = "2016",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Lab on A Chip",
title = "Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e"
}
Chen, Q., Utech, S., Chen, D., Prodanović, R., Lin, J.,& Weitz, D. A.. (2016). Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e. in Lab on A Chip
Royal Soc Chemistry, Cambridge..
Chen Q, Utech S, Chen D, Prodanović R, Lin J, Weitz DA. Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e. in Lab on A Chip. 2016;..
Chen, Qiushui, Utech, Stefanie, Chen, Dong, Prodanović, Radivoje, Lin, Jin-Ming, Weitz, David A., "Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e" in Lab on A Chip (2016).

Improved tag-switch method reveals that thioredoxin acts as depersulfidase and controls the intracellular levels of protein persulfidation

Wedmann, Rudolf; Onderka, Constantin; Wei, Shengwei; Szijarto, Istvan Andras; Miljković, Jan Lj; Mitrović, Aleksandra D.; Lange, Mike; Savitsky, Sergey; Yadav, Pramod Kumar; Torregrossa, Roberta; Harrer, Ellen G.; Harrer, Thomas; Ishii, Isao; Gollasch, Maik; Wood, Mark E.; Galardon, Erwan; Xian, Ming; Whiteman, Matthew; Banerjee, Ruma; Filipović, Miloš R.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Wedmann, Rudolf
AU  - Onderka, Constantin
AU  - Wei, Shengwei
AU  - Szijarto, Istvan Andras
AU  - Miljković, Jan Lj
AU  - Mitrović, Aleksandra D.
AU  - Lange, Mike
AU  - Savitsky, Sergey
AU  - Yadav, Pramod Kumar
AU  - Torregrossa, Roberta
AU  - Harrer, Ellen G.
AU  - Harrer, Thomas
AU  - Ishii, Isao
AU  - Gollasch, Maik
AU  - Wood, Mark E.
AU  - Galardon, Erwan
AU  - Xian, Ming
AU  - Whiteman, Matthew
AU  - Banerjee, Ruma
AU  - Filipović, Miloš R.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1925
AB  - Hydrogen sulfide (H2S) has emerged as a signalling molecule capable of regulating several important physiological functions such as blood pressure, neurotransmission and inflammation. The mechanisms behind these effects are still largely elusive and oxidative posttranslational modification of cysteine residues (protein persulfidation or S-sulfhydration) has been proposed as the main pathway for H2S-induced biological and pharmacological effects. As a signalling mechanism, persulfidation has to be controlled. Using an improved tag-switch assay for persulfide detection we show here that protein persulfide levels are controlled by the thioredoxin system. Recombinant thioredoxin showed an almost 10-fold higher reactivity towards cysteine persulfide than towards cystine and readily cleaved protein persulfides as well. This reaction resulted in H2S release suggesting that thioredoxin could be an important regulator of H2S levels from persulfide pools. Inhibition of the thioredoxin system caused an increase in intracellular persulfides, highlighting thioredoxin as a major protein depersulfidase that controls H2S signalling. Finally, using plasma from HIV-1 patients that have higher circulatory levels of thioredoxin, we could prove depersulfidase role in vivo.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Science
T1  - Improved tag-switch method reveals that thioredoxin acts as depersulfidase and controls the intracellular levels of protein persulfidation
VL  - 7
IS  - 5
SP  - 3414
EP  - 3426
DO  - 10.1039/c5sc04818d
UR  - Kon_3044
ER  - 
@article{
author = "Wedmann, Rudolf and Onderka, Constantin and Wei, Shengwei and Szijarto, Istvan Andras and Miljković, Jan Lj and Mitrović, Aleksandra D. and Lange, Mike and Savitsky, Sergey and Yadav, Pramod Kumar and Torregrossa, Roberta and Harrer, Ellen G. and Harrer, Thomas and Ishii, Isao and Gollasch, Maik and Wood, Mark E. and Galardon, Erwan and Xian, Ming and Whiteman, Matthew and Banerjee, Ruma and Filipović, Miloš R.",
year = "2016",
abstract = "Hydrogen sulfide (H2S) has emerged as a signalling molecule capable of regulating several important physiological functions such as blood pressure, neurotransmission and inflammation. The mechanisms behind these effects are still largely elusive and oxidative posttranslational modification of cysteine residues (protein persulfidation or S-sulfhydration) has been proposed as the main pathway for H2S-induced biological and pharmacological effects. As a signalling mechanism, persulfidation has to be controlled. Using an improved tag-switch assay for persulfide detection we show here that protein persulfide levels are controlled by the thioredoxin system. Recombinant thioredoxin showed an almost 10-fold higher reactivity towards cysteine persulfide than towards cystine and readily cleaved protein persulfides as well. This reaction resulted in H2S release suggesting that thioredoxin could be an important regulator of H2S levels from persulfide pools. Inhibition of the thioredoxin system caused an increase in intracellular persulfides, highlighting thioredoxin as a major protein depersulfidase that controls H2S signalling. Finally, using plasma from HIV-1 patients that have higher circulatory levels of thioredoxin, we could prove depersulfidase role in vivo.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Science",
title = "Improved tag-switch method reveals that thioredoxin acts as depersulfidase and controls the intracellular levels of protein persulfidation",
volume = "7",
number = "5",
pages = "3414-3426",
doi = "10.1039/c5sc04818d",
url = "Kon_3044"
}
Wedmann, R., Onderka, C., Wei, S., Szijarto, I. A., Miljković, J. L., Mitrović, A. D., Lange, M., Savitsky, S., Yadav, P. K., Torregrossa, R., Harrer, E. G., Harrer, T., Ishii, I., Gollasch, M., Wood, M. E., Galardon, E., Xian, M., Whiteman, M., Banerjee, R.,& Filipović, M. R.. (2016). Improved tag-switch method reveals that thioredoxin acts as depersulfidase and controls the intracellular levels of protein persulfidation. in Chemical Science
Royal Soc Chemistry, Cambridge., 7(5), 3414-3426.
https://doi.org/10.1039/c5sc04818d
Kon_3044
Wedmann R, Onderka C, Wei S, Szijarto IA, Miljković JL, Mitrović AD, Lange M, Savitsky S, Yadav PK, Torregrossa R, Harrer EG, Harrer T, Ishii I, Gollasch M, Wood ME, Galardon E, Xian M, Whiteman M, Banerjee R, Filipović MR. Improved tag-switch method reveals that thioredoxin acts as depersulfidase and controls the intracellular levels of protein persulfidation. in Chemical Science. 2016;7(5):3414-3426.
doi:10.1039/c5sc04818d
Kon_3044 .
Wedmann, Rudolf, Onderka, Constantin, Wei, Shengwei, Szijarto, Istvan Andras, Miljković, Jan Lj, Mitrović, Aleksandra D., Lange, Mike, Savitsky, Sergey, Yadav, Pramod Kumar, Torregrossa, Roberta, Harrer, Ellen G., Harrer, Thomas, Ishii, Isao, Gollasch, Maik, Wood, Mark E., Galardon, Erwan, Xian, Ming, Whiteman, Matthew, Banerjee, Ruma, Filipović, Miloš R., "Improved tag-switch method reveals that thioredoxin acts as depersulfidase and controls the intracellular levels of protein persulfidation" in Chemical Science, 7, no. 5 (2016):3414-3426,
https://doi.org/10.1039/c5sc04818d .,
Kon_3044 .
126
101
91

Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet

Chen, Qiushui; Utech, Stefanie; Chen, Dong; Prodanović, Radivoje; Lin, Jin-Ming; Weitz, David A.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Chen, Qiushui
AU  - Utech, Stefanie
AU  - Chen, Dong
AU  - Prodanović, Radivoje
AU  - Lin, Jin-Ming
AU  - Weitz, David A.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1917
AB  - This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.
PB  - Royal Soc Chemistry, Cambridge
T2  - Lab on A Chip
T1  - Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet
VL  - 16
IS  - 8
SP  - 1346
EP  - 1349
DO  - 10.1039/c6lc00231e
UR  - Kon_3036
ER  - 
@article{
author = "Chen, Qiushui and Utech, Stefanie and Chen, Dong and Prodanović, Radivoje and Lin, Jin-Ming and Weitz, David A.",
year = "2016",
abstract = "This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Lab on A Chip",
title = "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet",
volume = "16",
number = "8",
pages = "1346-1349",
doi = "10.1039/c6lc00231e",
url = "Kon_3036"
}
Chen, Q., Utech, S., Chen, D., Prodanović, R., Lin, J.,& Weitz, D. A.. (2016). Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet. in Lab on A Chip
Royal Soc Chemistry, Cambridge., 16(8), 1346-1349.
https://doi.org/10.1039/c6lc00231e
Kon_3036
Chen Q, Utech S, Chen D, Prodanović R, Lin J, Weitz DA. Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet. in Lab on A Chip. 2016;16(8):1346-1349.
doi:10.1039/c6lc00231e
Kon_3036 .
Chen, Qiushui, Utech, Stefanie, Chen, Dong, Prodanović, Radivoje, Lin, Jin-Ming, Weitz, David A., "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet" in Lab on A Chip, 16, no. 8 (2016):1346-1349,
https://doi.org/10.1039/c6lc00231e .,
Kon_3036 .
6
121
110
98

Supplementary material for the article: Wedmann, R.; Onderka, C.; Wei, S.; Szijártó, I. A.; Miljkovic, J. L.; Mitrovic, A.; Lange, M.; Savitsky, S.; Yadav, P. K.; Torregrossa, R.; et al. Improved Tag-Switch Method Reveals That Thioredoxin Acts as Depersulfidase and Controls the Intracellular Levels of Protein Persulfidation. Chemical Science 2016, 7 (5), 3414–3426. https://doi.org/10.1039/c5sc04818d

Wedmann, Rudolf; Onderka, Constantin; Wei, Shengwei; Szijarto, Istvan Andras; Miljković, Jan Lj; Mitrović, Aleksandra D.; Lange, Mike; Savitsky, Sergey; Yadav, Pramod Kumar; Torregrossa, Roberta; Harrer, Ellen G.; Harrer, Thomas; Ishii, Isao; Gollasch, Maik; Wood, Mark E.; Galardon, Erwan; Xian, Ming; Whiteman, Matthew; Banerjee, Ruma; Filipović, Miloš R.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - DATA
AU  - Wedmann, Rudolf
AU  - Onderka, Constantin
AU  - Wei, Shengwei
AU  - Szijarto, Istvan Andras
AU  - Miljković, Jan Lj
AU  - Mitrović, Aleksandra D.
AU  - Lange, Mike
AU  - Savitsky, Sergey
AU  - Yadav, Pramod Kumar
AU  - Torregrossa, Roberta
AU  - Harrer, Ellen G.
AU  - Harrer, Thomas
AU  - Ishii, Isao
AU  - Gollasch, Maik
AU  - Wood, Mark E.
AU  - Galardon, Erwan
AU  - Xian, Ming
AU  - Whiteman, Matthew
AU  - Banerjee, Ruma
AU  - Filipović, Miloš R.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3543
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Science
T1  - Supplementary material for the article: Wedmann, R.; Onderka, C.; Wei, S.; Szijártó, I. A.; Miljkovic, J. L.; Mitrovic, A.; Lange, M.; Savitsky, S.; Yadav, P. K.; Torregrossa, R.; et al. Improved Tag-Switch Method Reveals That Thioredoxin Acts as Depersulfidase and Controls the Intracellular Levels of Protein Persulfidation. Chemical Science 2016, 7 (5), 3414–3426. https://doi.org/10.1039/c5sc04818d
ER  - 
@misc{
author = "Wedmann, Rudolf and Onderka, Constantin and Wei, Shengwei and Szijarto, Istvan Andras and Miljković, Jan Lj and Mitrović, Aleksandra D. and Lange, Mike and Savitsky, Sergey and Yadav, Pramod Kumar and Torregrossa, Roberta and Harrer, Ellen G. and Harrer, Thomas and Ishii, Isao and Gollasch, Maik and Wood, Mark E. and Galardon, Erwan and Xian, Ming and Whiteman, Matthew and Banerjee, Ruma and Filipović, Miloš R.",
year = "2016",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Science",
title = "Supplementary material for the article: Wedmann, R.; Onderka, C.; Wei, S.; Szijártó, I. A.; Miljkovic, J. L.; Mitrovic, A.; Lange, M.; Savitsky, S.; Yadav, P. K.; Torregrossa, R.; et al. Improved Tag-Switch Method Reveals That Thioredoxin Acts as Depersulfidase and Controls the Intracellular Levels of Protein Persulfidation. Chemical Science 2016, 7 (5), 3414–3426. https://doi.org/10.1039/c5sc04818d"
}
Wedmann, R., Onderka, C., Wei, S., Szijarto, I. A., Miljković, J. L., Mitrović, A. D., Lange, M., Savitsky, S., Yadav, P. K., Torregrossa, R., Harrer, E. G., Harrer, T., Ishii, I., Gollasch, M., Wood, M. E., Galardon, E., Xian, M., Whiteman, M., Banerjee, R.,& Filipović, M. R.. (2016). Supplementary material for the article: Wedmann, R.; Onderka, C.; Wei, S.; Szijártó, I. A.; Miljkovic, J. L.; Mitrovic, A.; Lange, M.; Savitsky, S.; Yadav, P. K.; Torregrossa, R.; et al. Improved Tag-Switch Method Reveals That Thioredoxin Acts as Depersulfidase and Controls the Intracellular Levels of Protein Persulfidation. Chemical Science 2016, 7 (5), 3414–3426. https://doi.org/10.1039/c5sc04818d. in Chemical Science
Royal Soc Chemistry, Cambridge..
Wedmann R, Onderka C, Wei S, Szijarto IA, Miljković JL, Mitrović AD, Lange M, Savitsky S, Yadav PK, Torregrossa R, Harrer EG, Harrer T, Ishii I, Gollasch M, Wood ME, Galardon E, Xian M, Whiteman M, Banerjee R, Filipović MR. Supplementary material for the article: Wedmann, R.; Onderka, C.; Wei, S.; Szijártó, I. A.; Miljkovic, J. L.; Mitrovic, A.; Lange, M.; Savitsky, S.; Yadav, P. K.; Torregrossa, R.; et al. Improved Tag-Switch Method Reveals That Thioredoxin Acts as Depersulfidase and Controls the Intracellular Levels of Protein Persulfidation. Chemical Science 2016, 7 (5), 3414–3426. https://doi.org/10.1039/c5sc04818d. in Chemical Science. 2016;..
Wedmann, Rudolf, Onderka, Constantin, Wei, Shengwei, Szijarto, Istvan Andras, Miljković, Jan Lj, Mitrović, Aleksandra D., Lange, Mike, Savitsky, Sergey, Yadav, Pramod Kumar, Torregrossa, Roberta, Harrer, Ellen G., Harrer, Thomas, Ishii, Isao, Gollasch, Maik, Wood, Mark E., Galardon, Erwan, Xian, Ming, Whiteman, Matthew, Banerjee, Ruma, Filipović, Miloš R., "Supplementary material for the article: Wedmann, R.; Onderka, C.; Wei, S.; Szijártó, I. A.; Miljkovic, J. L.; Mitrovic, A.; Lange, M.; Savitsky, S.; Yadav, P. K.; Torregrossa, R.; et al. Improved Tag-Switch Method Reveals That Thioredoxin Acts as Depersulfidase and Controls the Intracellular Levels of Protein Persulfidation. Chemical Science 2016, 7 (5), 3414–3426. https://doi.org/10.1039/c5sc04818d" in Chemical Science (2016).

Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet

Chen, Qiushui; Utech, Stefanie; Chen, Dong; Prodanović, Radivoje; Lin, Jin-Ming; Weitz, David A.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Chen, Qiushui
AU  - Utech, Stefanie
AU  - Chen, Dong
AU  - Prodanović, Radivoje
AU  - Lin, Jin-Ming
AU  - Weitz, David A.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2820
AB  - This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.
PB  - Royal Soc Chemistry, Cambridge
T2  - Lab on A Chip
T1  - Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet
VL  - 16
IS  - 8
SP  - 1346
EP  - 1349
DO  - 10.1039/c6lc00231e
ER  - 
@article{
author = "Chen, Qiushui and Utech, Stefanie and Chen, Dong and Prodanović, Radivoje and Lin, Jin-Ming and Weitz, David A.",
year = "2016",
abstract = "This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Lab on A Chip",
title = "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet",
volume = "16",
number = "8",
pages = "1346-1349",
doi = "10.1039/c6lc00231e"
}
Chen, Q., Utech, S., Chen, D., Prodanović, R., Lin, J.,& Weitz, D. A.. (2016). Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet. in Lab on A Chip
Royal Soc Chemistry, Cambridge., 16(8), 1346-1349.
https://doi.org/10.1039/c6lc00231e
Chen Q, Utech S, Chen D, Prodanović R, Lin J, Weitz DA. Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet. in Lab on A Chip. 2016;16(8):1346-1349.
doi:10.1039/c6lc00231e .
Chen, Qiushui, Utech, Stefanie, Chen, Dong, Prodanović, Radivoje, Lin, Jin-Ming, Weitz, David A., "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet" in Lab on A Chip, 16, no. 8 (2016):1346-1349,
https://doi.org/10.1039/c6lc00231e . .
6
121
110
99