Harvard MRSEC [DMR-0820484]

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Harvard MRSEC [DMR-0820484]

Authors

Publications

Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

(Wiley-Blackwell, Hoboken, 2015)

TY  - DATA
AU  - Utech, Stefanie
AU  - Prodanović, Radivoje
AU  - Mao, Angelo S.
AU  - Ostafe, Raluca
AU  - Mooney, David J.
AU  - Weitz, David A.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3383
PB  - Wiley-Blackwell, Hoboken
T2  - Advanced Healthcare Materials
T1  - Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021
ER  - 
@misc{
author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Advanced Healthcare Materials",
title = "Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021"
}
Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A.. (2015). Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021. in Advanced Healthcare Materials
Wiley-Blackwell, Hoboken..
Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021. in Advanced Healthcare Materials. 2015;..
Utech, Stefanie, Prodanović, Radivoje, Mao, Angelo S., Ostafe, Raluca, Mooney, David J., Weitz, David A., "Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021" in Advanced Healthcare Materials (2015).

Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

(Wiley-Blackwell, Hoboken, 2015)

TY  - JOUR
AU  - Utech, Stefanie
AU  - Prodanović, Radivoje
AU  - Mao, Angelo S.
AU  - Ostafe, Raluca
AU  - Mooney, David J.
AU  - Weitz, David A.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2785
AB  - Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
PB  - Wiley-Blackwell, Hoboken
T2  - Advanced Healthcare Materials
T1  - Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
VL  - 4
IS  - 11
SP  - 1628
EP  - 1633
DO  - 10.1002/adhm.201500021
ER  - 
@article{
author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.",
year = "2015",
abstract = "Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Advanced Healthcare Materials",
title = "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture",
volume = "4",
number = "11",
pages = "1628-1633",
doi = "10.1002/adhm.201500021"
}
Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A.. (2015). Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials
Wiley-Blackwell, Hoboken., 4(11), 1628-1633.
https://doi.org/10.1002/adhm.201500021
Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials. 2015;4(11):1628-1633.
doi:10.1002/adhm.201500021 .
Utech, Stefanie, Prodanović, Radivoje, Mao, Angelo S., Ostafe, Raluca, Mooney, David J., Weitz, David A., "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture" in Advanced Healthcare Materials, 4, no. 11 (2015):1628-1633,
https://doi.org/10.1002/adhm.201500021 . .
189
167
175

Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

(Wiley-Blackwell, Hoboken, 2015)

TY  - JOUR
AU  - Utech, Stefanie
AU  - Prodanović, Radivoje
AU  - Mao, Angelo S.
AU  - Ostafe, Raluca
AU  - Mooney, David J.
AU  - Weitz, David A.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1751
PB  - Wiley-Blackwell, Hoboken
T2  - Advanced Healthcare Materials
T1  - Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
VL  - 4
IS  - 11
SP  - 1628
EP  - 1633
DO  - 10.1002/adhm.201500021
UR  - Kon_2897
ER  - 
@article{
author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Advanced Healthcare Materials",
title = "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture",
volume = "4",
number = "11",
pages = "1628-1633",
doi = "10.1002/adhm.201500021",
url = "Kon_2897"
}
Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A.. (2015). Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials
Wiley-Blackwell, Hoboken., 4(11), 1628-1633.
https://doi.org/10.1002/adhm.201500021
Kon_2897
Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. in Advanced Healthcare Materials. 2015;4(11):1628-1633.
doi:10.1002/adhm.201500021
Kon_2897 .
Utech, Stefanie, Prodanović, Radivoje, Mao, Angelo S., Ostafe, Raluca, Mooney, David J., Weitz, David A., "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture" in Advanced Healthcare Materials, 4, no. 11 (2015):1628-1633,
https://doi.org/10.1002/adhm.201500021 .,
Kon_2897 .
189
167
176

Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771

Ostafe, Raluca; Prodanović, Radivoje; Ung, Lloyd W.; Weitz, David A.; Fischer, Rainer

(Amer Inst Physics, Melville, 2014)

TY  - DATA
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Ung, Lloyd W.
AU  - Weitz, David A.
AU  - Fischer, Rainer
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3666
PB  - Amer Inst Physics, Melville
T2  - Biomicrofluidics
T1  - Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771
ER  - 
@misc{
author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer",
year = "2014",
publisher = "Amer Inst Physics, Melville",
journal = "Biomicrofluidics",
title = "Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771"
}
Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R.. (2014). Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771. in Biomicrofluidics
Amer Inst Physics, Melville..
Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771. in Biomicrofluidics. 2014;..
Ostafe, Raluca, Prodanović, Radivoje, Ung, Lloyd W., Weitz, David A., Fischer, Rainer, "Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771" in Biomicrofluidics (2014).

A high-throughput cellulase screening system based on droplet microfluidics

Ostafe, Raluca; Prodanović, Radivoje; Ung, Lloyd W.; Weitz, David A.; Fischer, Rainer

(Amer Inst Physics, Melville, 2014)

TY  - JOUR
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Ung, Lloyd W.
AU  - Weitz, David A.
AU  - Fischer, Rainer
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1873
AB  - A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
PB  - Amer Inst Physics, Melville
T2  - Biomicrofluidics
T1  - A high-throughput cellulase screening system based on droplet microfluidics
VL  - 8
IS  - 4
DO  - 10.1063/1.4886771
UR  - Kon_2756
ER  - 
@article{
author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer",
year = "2014",
abstract = "A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.",
publisher = "Amer Inst Physics, Melville",
journal = "Biomicrofluidics",
title = "A high-throughput cellulase screening system based on droplet microfluidics",
volume = "8",
number = "4",
doi = "10.1063/1.4886771",
url = "Kon_2756"
}
Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R.. (2014). A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics
Amer Inst Physics, Melville., 8(4).
https://doi.org/10.1063/1.4886771
Kon_2756
Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics. 2014;8(4).
doi:10.1063/1.4886771
Kon_2756 .
Ostafe, Raluca, Prodanović, Radivoje, Ung, Lloyd W., Weitz, David A., Fischer, Rainer, "A high-throughput cellulase screening system based on droplet microfluidics" in Biomicrofluidics, 8, no. 4 (2014),
https://doi.org/10.1063/1.4886771 .,
Kon_2756 .
4
44
38
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