TY  - CONF
AU  - Ivković, Đurđa
AU  - Topić, Milica
AU  - Trifković, Jelena
AU  - Krstić-Ristivojević, Maja
AU  - Ristivojević, Petar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6219
AB  - Due to their strong antioxidative potential, flower petals are considered important starting materials for the development of cosmetic products1. Present study explored phenolic profile and antioxidative potential of 17 flower petals and their potential application in the cosmetics industry. The phenolic profile of investigated extracts was determined using High-Performance Thin Layer Chromatography (HPTLC). The antioxidative potential was assessed using both ABTS and DPPH spectrophotometric tests, and total phenol content. DPPH-HPTLC assay was applied to identify compounds with the highest antioxidative activity. Five phenols were identified in the investigated extracts. Based on radical antioxidative tests, Paeonia lactifora, Paeonia decora, Rosa istriaca and Pelargonium peltatum were identified as species with the highest antioxidative potential, while gallic acid was recognized as the strongest antioxidants.

1. D. F. Hernandez et all, Crit. Rev. Food. Sci. Nutr, 2021, 61, 3740-3755.

Acknowledgement: This work was supported by The Science Fund of the Republic of Serbia, Serbian Science and Diaspora Collaboration Program, No.6389927.
C3  - 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia
T1  - The phenolic profile and antioxidative activity of seventeen flower petals extracts
IS  - 44
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6219
ER  - 

@conference{
author = "Ivković, Đurđa and Topić, Milica and Trifković, Jelena and Krstić-Ristivojević, Maja and Ristivojević, Petar",
year = "2023",
abstract = "Due to their strong antioxidative potential, flower petals are considered important starting materials for the development of cosmetic products1. Present study explored phenolic profile and antioxidative potential of 17 flower petals and their potential application in the cosmetics industry. The phenolic profile of investigated extracts was determined using High-Performance Thin Layer Chromatography (HPTLC). The antioxidative potential was assessed using both ABTS and DPPH spectrophotometric tests, and total phenol content. DPPH-HPTLC assay was applied to identify compounds with the highest antioxidative activity. Five phenols were identified in the investigated extracts. Based on radical antioxidative tests, Paeonia lactifora, Paeonia decora, Rosa istriaca and Pelargonium peltatum were identified as species with the highest antioxidative potential, while gallic acid was recognized as the strongest antioxidants.

1. D. F. Hernandez et all, Crit. Rev. Food. Sci. Nutr, 2021, 61, 3740-3755.

Acknowledgement: This work was supported by The Science Fund of the Republic of Serbia, Serbian Science and Diaspora Collaboration Program, No.6389927.",
journal = "59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia",
title = "The phenolic profile and antioxidative activity of seventeen flower petals extracts",
number = "44",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6219"
}

Ivković, Đ., Topić, M., Trifković, J., Krstić-Ristivojević, M.,& Ristivojević, P.. (2023). The phenolic profile and antioxidative activity of seventeen flower petals extracts. in 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia(44).
https://hdl.handle.net/21.15107/rcub_cherry_6219

Ivković Đ, Topić M, Trifković J, Krstić-Ristivojević M, Ristivojević P. The phenolic profile and antioxidative activity of seventeen flower petals extracts. in 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia. 2023;(44).
https://hdl.handle.net/21.15107/rcub_cherry_6219 .

Ivković, Đurđa, Topić, Milica, Trifković, Jelena, Krstić-Ristivojević, Maja, Ristivojević, Petar, "The phenolic profile and antioxidative activity of seventeen flower petals extracts" in 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia, no. 44 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6219 .

TY  - CONF
AU  - Ivković, Đurđa
AU  - Ristivojević, Petar
AU  - Krstić-Ristivojević, Maja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6224
AB  - The cosmetic industry is currently experiencing substantial growth, accompanied by a surge in the number of consumers. Due to natural ingredients' safety and efficiency, an increasingly prominent trend in this industry is the design of herbal-based cosmetic products and formulations. To meet demand, integrating innovative natural resources like plant-based formulations is essential [1]. Skin ageing-related enzymes, elastase and tyrosinase, contribute to the emergence of aesthetic issues such as wrinkles, freckles, and loss of elasticity, as well as hyperpigmentation and melasma [2]. Hence, there is an increasing focus on finding inhibitors for enzymes associated with skin ageing. The fact is that plant metabolites, particularly phenolic acids and flavonoids, are frequently concentrated in plant leaves and flowers. Accordingly, we assessed the inhibitory effects on elastase and tyrosinase in seventeen particularly selected methanolic extracts obtained from various flower petal varieties cultivated in Serbia. Our analysis revealed that petals exhibit significantly more pronounced inhibitory effects, expressed as IC50 values, on elastase compared to tyrosinase. White peony (Paeonia lactifora) (230 ± 1 μg/mL), pelargonium (Pelargonium peltatum) (206 ± 3 μg/mL) and purple rose (Rosa centifolia, purple) (222 ± 8 μg/mL) emerged as the most potent elastase inhibitors, surpassing the reference compound epigallocatechin gallate (348 ± 9 μg/mL). Additionally, the lowest IC50 value for tyrosinase inhibition was attributed to lilac (Syringa vulgaris) petal extract (272 ± 28 μg/mL), which was compared to the standard compound kojic acid (50 ± 14 μg/mL). In conclusion, white peony, pelargonium, purple rose, and lilac represent promising candidates for further investigation and incorporation into cosmetic products, showcasing their potential to effectively address skin-related concerns.

References
1. C. Lourenço-Lopes, M. Fraga-Corral, M. Carpena, Resour. 2020, 9, 101.
2. I. Chiocchio, M. Mandrone, C. Sanna, A. Maxia, M. Tacchini, F. Poli, Ind. Crops Prod. 2018, 122, 498.
Acknowledgements

This work was supported by The Science Fund of the Republic of Serbia, Serbian Science and Diaspora Collaboration Program, No. 6389927, Ministry of Science, Technological Development and Innovation of Republic of Serbia Contract numbers 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Elastase and tyrosinase inhibitory activity of plant petals cultivated in Serbia
IS  - 132
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6224
ER  - 

@conference{
author = "Ivković, Đurđa and Ristivojević, Petar and Krstić-Ristivojević, Maja",
year = "2023",
abstract = "The cosmetic industry is currently experiencing substantial growth, accompanied by a surge in the number of consumers. Due to natural ingredients' safety and efficiency, an increasingly prominent trend in this industry is the design of herbal-based cosmetic products and formulations. To meet demand, integrating innovative natural resources like plant-based formulations is essential [1]. Skin ageing-related enzymes, elastase and tyrosinase, contribute to the emergence of aesthetic issues such as wrinkles, freckles, and loss of elasticity, as well as hyperpigmentation and melasma [2]. Hence, there is an increasing focus on finding inhibitors for enzymes associated with skin ageing. The fact is that plant metabolites, particularly phenolic acids and flavonoids, are frequently concentrated in plant leaves and flowers. Accordingly, we assessed the inhibitory effects on elastase and tyrosinase in seventeen particularly selected methanolic extracts obtained from various flower petal varieties cultivated in Serbia. Our analysis revealed that petals exhibit significantly more pronounced inhibitory effects, expressed as IC50 values, on elastase compared to tyrosinase. White peony (Paeonia lactifora) (230 ± 1 μg/mL), pelargonium (Pelargonium peltatum) (206 ± 3 μg/mL) and purple rose (Rosa centifolia, purple) (222 ± 8 μg/mL) emerged as the most potent elastase inhibitors, surpassing the reference compound epigallocatechin gallate (348 ± 9 μg/mL). Additionally, the lowest IC50 value for tyrosinase inhibition was attributed to lilac (Syringa vulgaris) petal extract (272 ± 28 μg/mL), which was compared to the standard compound kojic acid (50 ± 14 μg/mL). In conclusion, white peony, pelargonium, purple rose, and lilac represent promising candidates for further investigation and incorporation into cosmetic products, showcasing their potential to effectively address skin-related concerns.

References
1. C. Lourenço-Lopes, M. Fraga-Corral, M. Carpena, Resour. 2020, 9, 101.
2. I. Chiocchio, M. Mandrone, C. Sanna, A. Maxia, M. Tacchini, F. Poli, Ind. Crops Prod. 2018, 122, 498.
Acknowledgements

This work was supported by The Science Fund of the Republic of Serbia, Serbian Science and Diaspora Collaboration Program, No. 6389927, Ministry of Science, Technological Development and Innovation of Republic of Serbia Contract numbers 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Elastase and tyrosinase inhibitory activity of plant petals cultivated in Serbia",
number = "132",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6224"
}

Ivković, Đ., Ristivojević, P.,& Krstić-Ristivojević, M.. (2023). Elastase and tyrosinase inhibitory activity of plant petals cultivated in Serbia. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science(132).
https://hdl.handle.net/21.15107/rcub_cherry_6224

Ivković Đ, Ristivojević P, Krstić-Ristivojević M. Elastase and tyrosinase inhibitory activity of plant petals cultivated in Serbia. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;(132).
https://hdl.handle.net/21.15107/rcub_cherry_6224 .

Ivković, Đurđa, Ristivojević, Petar, Krstić-Ristivojević, Maja, "Elastase and tyrosinase inhibitory activity of plant petals cultivated in Serbia" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science, no. 132 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6224 .