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Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636

Penjišević, Jelena ; Šukalović, Vladimir ; Dukić-Stefanović, Slađana; Deuther-Conrad, Winnie; Andrić, Deana ; Kostić-Rajačić, Slađana

TY  - DATA
AU  - Penjišević, Jelena 
AU  - Šukalović, Vladimir 
AU  - Dukić-Stefanović, Slađana
AU  - Deuther-Conrad, Winnie
AU  - Andrić, Deana 
AU  - Kostić-Rajačić, Slađana
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5845
AB  - Serotonin receptors modulate numerous behavioral and neuropsychological processes. Therefore, they are the target for the action of many drugs, such as antipsychotics, antidepressants, antiemetics, migraine remedies, and many others. The 5-HT1A receptors have been involved in the pathogenesis and treatment of anxiety and depression and represent a promising target for new drugs with reduced extrapyramidal side effects. In most antidepressants, a piperazine-based structural motif can be identified as a common moiety. Here we describe the synthesis, pharmacological, and in silico characterization of a novel arylpiperazines series with excellent 5-HT1A affinity. The final compounds, 4a, 8a, and 8b, were selected according to predictions of in silico pharmacokinetics, docking analysis, and molecular dynamics in conjunction with physical properties, and metabolic stability. The accentuated molecules could serve as a lead compound for developing 5-HT1A drug-like molecules for depression treatment.
PB  - Elsevier
T2  - Arabian Journal of Chemistry
T1  - Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636
VL  - 16
IS  - 4
SP  - 104636
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5845
ER  - 
@misc{
author = "Penjišević, Jelena  and Šukalović, Vladimir  and Dukić-Stefanović, Slađana and Deuther-Conrad, Winnie and Andrić, Deana  and Kostić-Rajačić, Slađana",
abstract = "Serotonin receptors modulate numerous behavioral and neuropsychological processes. Therefore, they are the target for the action of many drugs, such as antipsychotics, antidepressants, antiemetics, migraine remedies, and many others. The 5-HT1A receptors have been involved in the pathogenesis and treatment of anxiety and depression and represent a promising target for new drugs with reduced extrapyramidal side effects. In most antidepressants, a piperazine-based structural motif can be identified as a common moiety. Here we describe the synthesis, pharmacological, and in silico characterization of a novel arylpiperazines series with excellent 5-HT1A affinity. The final compounds, 4a, 8a, and 8b, were selected according to predictions of in silico pharmacokinetics, docking analysis, and molecular dynamics in conjunction with physical properties, and metabolic stability. The accentuated molecules could serve as a lead compound for developing 5-HT1A drug-like molecules for depression treatment.",
publisher = "Elsevier",
journal = "Arabian Journal of Chemistry",
title = "Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636",
volume = "16",
number = "4",
pages = "104636",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5845"
}
Penjišević, J., Šukalović, V., Dukić-Stefanović, S., Deuther-Conrad, W., Andrić, D.,& Kostić-Rajačić, S..Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636. in Arabian Journal of Chemistry
Elsevier., 16(4), 104636.
https://hdl.handle.net/21.15107/rcub_cherry_5845
Penjišević J, Šukalović V, Dukić-Stefanović S, Deuther-Conrad W, Andrić D, Kostić-Rajačić S. Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636. in Arabian Journal of Chemistry.16(4):104636.
https://hdl.handle.net/21.15107/rcub_cherry_5845 .
Penjišević, Jelena , Šukalović, Vladimir , Dukić-Stefanović, Slađana, Deuther-Conrad, Winnie, Andrić, Deana , Kostić-Rajačić, Slađana, "Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636" in Arabian Journal of Chemistry, 16, no. 4:104636,
https://hdl.handle.net/21.15107/rcub_cherry_5845 .

Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry Royal Society of Chemistry., 21(6), 1187-1205. https://doi.org/10.1039/D2OB02119F

Aleksić, Jovana; Stojanović, Milovan; Bošković, Jakša; Baranac-Stojanović, Marija

TY  - DATA
AU  - Aleksić, Jovana
AU  - Stojanović, Milovan
AU  - Bošković, Jakša
AU  - Baranac-Stojanović, Marija
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5846
AB  - We present the green, highly atom-economical, solid-state silica gel-catalyzed synthesis of polysubstituted 1,4- and 1,2-dihydropyridines (DHPs) from commercially available materials, amines and ethyl propiolate. The DHP skeleton was assembled by heating the reactants and silica gel in a closed vessel. Aliphatic amines provided 1,4-isomers as the main or only DHP products, but the reactions of aromatic amines yielded a mixture of 1,4- and 1,2-isomers. To the best of our knowledge, this is the first example of the formation of a 1,2-DHP structure by the reaction of an amine with propiolic ester. Addition of 1 mass percent of H2SO4 to silica gel shifted the product distribution to 1,4-DHP as the main or the only isomer obtained. Experimental and theoretical analyses led to the identification of two key intermediates en route to DHPs and the explanation of the observed regioisomeric ratios. 1,2-DHPs show blue-cyan fluorescence in MeOH with the quantum yield Φ = 0.10–0.22 relative to quinine sulfate Φ = 0.58 and 1,4-DHPs show blue-violet fluorescence with Φ = 0.09–0.81.
PB  - Royal Society of Chemistry
T2  - Organic & Biomolecular Chemistry
T1  - Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F
VL  - 21
IS  - 6
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5846
ER  - 
@misc{
author = "Aleksić, Jovana and Stojanović, Milovan and Bošković, Jakša and Baranac-Stojanović, Marija",
abstract = "We present the green, highly atom-economical, solid-state silica gel-catalyzed synthesis of polysubstituted 1,4- and 1,2-dihydropyridines (DHPs) from commercially available materials, amines and ethyl propiolate. The DHP skeleton was assembled by heating the reactants and silica gel in a closed vessel. Aliphatic amines provided 1,4-isomers as the main or only DHP products, but the reactions of aromatic amines yielded a mixture of 1,4- and 1,2-isomers. To the best of our knowledge, this is the first example of the formation of a 1,2-DHP structure by the reaction of an amine with propiolic ester. Addition of 1 mass percent of H2SO4 to silica gel shifted the product distribution to 1,4-DHP as the main or the only isomer obtained. Experimental and theoretical analyses led to the identification of two key intermediates en route to DHPs and the explanation of the observed regioisomeric ratios. 1,2-DHPs show blue-cyan fluorescence in MeOH with the quantum yield Φ = 0.10–0.22 relative to quinine sulfate Φ = 0.58 and 1,4-DHPs show blue-violet fluorescence with Φ = 0.09–0.81.",
publisher = "Royal Society of Chemistry",
journal = "Organic & Biomolecular Chemistry",
title = "Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F",
volume = "21",
number = "6",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5846"
}
Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M..Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6).
https://hdl.handle.net/21.15107/rcub_cherry_5846
Aleksić J, Stojanović M, Bošković J, Baranac-Stojanović M. Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F. in Organic & Biomolecular Chemistry.21(6).
https://hdl.handle.net/21.15107/rcub_cherry_5846 .
Aleksić, Jovana, Stojanović, Milovan, Bošković, Jakša, Baranac-Stojanović, Marija, "Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F" in Organic & Biomolecular Chemistry, 21, no. 6,
https://hdl.handle.net/21.15107/rcub_cherry_5846 .

Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen

Đurašinović, Tatjana; Lopandić, Zorana; Protić-Rosić, Isidora; Nešić, Andrijana; Trbojević-Ivić, Jovana; Jappe, Uta; Gavrović-Jankulović, Marija

(Elsevier, 2024)

TY  - JOUR
AU  - Đurašinović, Tatjana
AU  - Lopandić, Zorana
AU  - Protić-Rosić, Isidora
AU  - Nešić, Andrijana
AU  - Trbojević-Ivić, Jovana
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6338
AB  - Banana allergy is often associated with the pollen and latex allergies, which led us to the  hypothesis that some yet unidentified banana allergen could provide a basis of the latex-pollen-fruit syndrome. S-adenosyl-L-homocysteine hydrolase (SAHH) was recently identified in the literature as a novel plant allergen. This study aimed to assess the allergenic potential of the naturally occurring banana SAHH (nSAHH) and its recombinant homolog produced in E. coli (rSAHH). nSAHH showed IgE reactivity with a serum pool of twelve banana-allergic persons,
while rSAHH displayed IgE reactivity in ten out of the twelve tested patients. Five linear B-cell epitopes were identified on the rSAHH surface, exhibiting ≥ 90 % sequence homology with relevant plant SAHH allergens. Our findings have elucidated SAHH as a novel plant panallergen, underlying the cross-reactivity between plant derived food and respiratory allergens, confirming our initial hypothesis.
PB  - Elsevier
T2  - Food Chemistry
T1  - Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen
VL  - 437
SP  - 137782
DO  - 10.1016/j.foodchem.2023.137782
ER  - 
@article{
author = "Đurašinović, Tatjana and Lopandić, Zorana and Protić-Rosić, Isidora and Nešić, Andrijana and Trbojević-Ivić, Jovana and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2024",
abstract = "Banana allergy is often associated with the pollen and latex allergies, which led us to the  hypothesis that some yet unidentified banana allergen could provide a basis of the latex-pollen-fruit syndrome. S-adenosyl-L-homocysteine hydrolase (SAHH) was recently identified in the literature as a novel plant allergen. This study aimed to assess the allergenic potential of the naturally occurring banana SAHH (nSAHH) and its recombinant homolog produced in E. coli (rSAHH). nSAHH showed IgE reactivity with a serum pool of twelve banana-allergic persons,
while rSAHH displayed IgE reactivity in ten out of the twelve tested patients. Five linear B-cell epitopes were identified on the rSAHH surface, exhibiting ≥ 90 % sequence homology with relevant plant SAHH allergens. Our findings have elucidated SAHH as a novel plant panallergen, underlying the cross-reactivity between plant derived food and respiratory allergens, confirming our initial hypothesis.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen",
volume = "437",
pages = "137782",
doi = "10.1016/j.foodchem.2023.137782"
}
Đurašinović, T., Lopandić, Z., Protić-Rosić, I., Nešić, A., Trbojević-Ivić, J., Jappe, U.,& Gavrović-Jankulović, M.. (2024). Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen. in Food Chemistry
Elsevier., 437, 137782.
https://doi.org/10.1016/j.foodchem.2023.137782
Đurašinović T, Lopandić Z, Protić-Rosić I, Nešić A, Trbojević-Ivić J, Jappe U, Gavrović-Jankulović M. Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen. in Food Chemistry. 2024;437:137782.
doi:10.1016/j.foodchem.2023.137782 .
Đurašinović, Tatjana, Lopandić, Zorana, Protić-Rosić, Isidora, Nešić, Andrijana, Trbojević-Ivić, Jovana, Jappe, Uta, Gavrović-Jankulović, Marija, "Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen" in Food Chemistry, 437 (2024):137782,
https://doi.org/10.1016/j.foodchem.2023.137782 . .

Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects

Milenković, Mila; Ciasca, Gabriele; Bonasera, Aurelio; Scopelliti, Michelangelo; Marković, Olivera; Verbić, Tatjana; Todorović Marković, Biljana; Jovanović, Svetlana

(Elsevier, 2024)

TY  - JOUR
AU  - Milenković, Mila
AU  - Ciasca, Gabriele
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Todorović Marković, Biljana
AU  - Jovanović, Svetlana
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6303
AB  - The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give 
in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, 
smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the 
antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. 
Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic 
resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of 
modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in 
the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol 
groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 
4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed 
that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs 
combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 
sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against 
S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.
PB  - Elsevier
T2  - Journal of Photochemistry & Photobiology, B: Biology
T1  - Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects
VL  - 250
SP  - 112818
DO  - 10.1016/j.jphotobiol.2023.112818
ER  - 
@article{
author = "Milenković, Mila and Ciasca, Gabriele and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Todorović Marković, Biljana and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give 
in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, 
smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the 
antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. 
Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic 
resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of 
modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in 
the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol 
groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 
4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed 
that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs 
combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 
sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against 
S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.",
publisher = "Elsevier",
journal = "Journal of Photochemistry & Photobiology, B: Biology",
title = "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects",
volume = "250",
pages = "112818",
doi = "10.1016/j.jphotobiol.2023.112818"
}
Milenković, M., Ciasca, G., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Todorović Marković, B.,& Jovanović, S.. (2024). Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects. in Journal of Photochemistry & Photobiology, B: Biology
Elsevier., 250, 112818.
https://doi.org/10.1016/j.jphotobiol.2023.112818
Milenković M, Ciasca G, Bonasera A, Scopelliti M, Marković O, Verbić T, Todorović Marković B, Jovanović S. Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects. in Journal of Photochemistry & Photobiology, B: Biology. 2024;250:112818.
doi:10.1016/j.jphotobiol.2023.112818 .
Milenković, Mila, Ciasca, Gabriele, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Todorović Marković, Biljana, Jovanović, Svetlana, "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects" in Journal of Photochemistry & Photobiology, B: Biology, 250 (2024):112818,
https://doi.org/10.1016/j.jphotobiol.2023.112818 . .

The Effect of Rare-Earth Elements on the Morphological Aspect of Borate and Electrocatalytic Sensing of Biological Compounds

Morozov, Roman; Stanković, Dalibor; Avdin, Viacheslav; Zherebtsov, Dmitri; Romashov, Mikhail; Selezneva, Anastasia; Uchaev, Daniil; Senin, Anatoly; Chernukha, Alexander

(MDPI, 2023)

TY  - JOUR
AU  - Morozov, Roman
AU  - Stanković, Dalibor
AU  - Avdin, Viacheslav
AU  - Zherebtsov, Dmitri
AU  - Romashov, Mikhail
AU  - Selezneva, Anastasia
AU  - Uchaev, Daniil
AU  - Senin, Anatoly
AU  - Chernukha, Alexander
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6382
AB  - Adjusting the morphological characteristics of a material can result in improved electrocatalytic capabilities of the material itself. An example of this is the introduction of rare-earth elements into the borate structure, which gives a new perspective on the possibilities of this type of material in the field of (bio)sensing. In this paper, we present the preparation of borates including La, Nd and Dy and their application for the modification of a glassy carbon electrode, which is used for the non-enzymatic detection of a biologically relevant molecule, vitamin B6 (pyridoxine). Compared with the others, dysprosium borate has the best electrocatalytic performance, showing the highest current and the lowest impedance, respectively, as determined using cyclic voltammetry and impedance tests. Quantitative testing of B6 was performed in DPV mode in a Britton–Robinson buffer solution with a pH of 6 and an oxidation potential of about +0.8 V. The calibration graph for the evaluation of B6 has a linear range from 1 to 100 μM, with a correlation coefficient of 0.9985 and a detection limit of 0.051 μM. The DyBO3-modified electrode can be used repeatedly, retaining more than 90% of the initial signal level after six cycles. The satisfactory selectivity offered a potential practical application of the chosen method for the monitoring of pyridoxine in artificially prepared biological fluids with acceptable recovery. In light of all the obtained results, this paper shows an important approach for the successful design of electrocatalysts with tuned architecture and opens new strategies for the development of materials for the needs of electrochemical (bio)sensing.
PB  - MDPI
T2  - Biosensors
T1  - The Effect of Rare-Earth Elements on the Morphological Aspect of Borate and Electrocatalytic Sensing of Biological Compounds
VL  - 13
IS  - 10
SP  - 901
DO  - 10.3390/bios13100901
ER  - 
@article{
author = "Morozov, Roman and Stanković, Dalibor and Avdin, Viacheslav and Zherebtsov, Dmitri and Romashov, Mikhail and Selezneva, Anastasia and Uchaev, Daniil and Senin, Anatoly and Chernukha, Alexander",
year = "2023",
abstract = "Adjusting the morphological characteristics of a material can result in improved electrocatalytic capabilities of the material itself. An example of this is the introduction of rare-earth elements into the borate structure, which gives a new perspective on the possibilities of this type of material in the field of (bio)sensing. In this paper, we present the preparation of borates including La, Nd and Dy and their application for the modification of a glassy carbon electrode, which is used for the non-enzymatic detection of a biologically relevant molecule, vitamin B6 (pyridoxine). Compared with the others, dysprosium borate has the best electrocatalytic performance, showing the highest current and the lowest impedance, respectively, as determined using cyclic voltammetry and impedance tests. Quantitative testing of B6 was performed in DPV mode in a Britton–Robinson buffer solution with a pH of 6 and an oxidation potential of about +0.8 V. The calibration graph for the evaluation of B6 has a linear range from 1 to 100 μM, with a correlation coefficient of 0.9985 and a detection limit of 0.051 μM. The DyBO3-modified electrode can be used repeatedly, retaining more than 90% of the initial signal level after six cycles. The satisfactory selectivity offered a potential practical application of the chosen method for the monitoring of pyridoxine in artificially prepared biological fluids with acceptable recovery. In light of all the obtained results, this paper shows an important approach for the successful design of electrocatalysts with tuned architecture and opens new strategies for the development of materials for the needs of electrochemical (bio)sensing.",
publisher = "MDPI",
journal = "Biosensors",
title = "The Effect of Rare-Earth Elements on the Morphological Aspect of Borate and Electrocatalytic Sensing of Biological Compounds",
volume = "13",
number = "10",
pages = "901",
doi = "10.3390/bios13100901"
}
Morozov, R., Stanković, D., Avdin, V., Zherebtsov, D., Romashov, M., Selezneva, A., Uchaev, D., Senin, A.,& Chernukha, A.. (2023). The Effect of Rare-Earth Elements on the Morphological Aspect of Borate and Electrocatalytic Sensing of Biological Compounds. in Biosensors
MDPI., 13(10), 901.
https://doi.org/10.3390/bios13100901
Morozov R, Stanković D, Avdin V, Zherebtsov D, Romashov M, Selezneva A, Uchaev D, Senin A, Chernukha A. The Effect of Rare-Earth Elements on the Morphological Aspect of Borate and Electrocatalytic Sensing of Biological Compounds. in Biosensors. 2023;13(10):901.
doi:10.3390/bios13100901 .
Morozov, Roman, Stanković, Dalibor, Avdin, Viacheslav, Zherebtsov, Dmitri, Romashov, Mikhail, Selezneva, Anastasia, Uchaev, Daniil, Senin, Anatoly, Chernukha, Alexander, "The Effect of Rare-Earth Elements on the Morphological Aspect of Borate and Electrocatalytic Sensing of Biological Compounds" in Biosensors, 13, no. 10 (2023):901,
https://doi.org/10.3390/bios13100901 . .

Nickel(II) and nickel(III) Thiosemicarbazone and Hydrazone Complexes: An Unexpected Journey

Jevtović, Mima; Pevec, Andrej; Turel, Iztok; Radanović, Dušanka; Milčić, Miloš; Gruden, Maja; Zlatar, Matija; Mitić, Dragana; Anđelković, Katarina; Čobeljić, Božidar

(Elsevier, 2023)

TY  - JOUR
AU  - Jevtović, Mima
AU  - Pevec, Andrej
AU  - Turel, Iztok
AU  - Radanović, Dušanka
AU  - Milčić, Miloš
AU  - Gruden, Maja
AU  - Zlatar, Matija
AU  - Mitić, Dragana
AU  - Anđelković, Katarina
AU  - Čobeljić, Božidar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6381
AB  - In this paper, we report the  synthesis, characterization, and X-ray structure analysis of one octahedral Ni(II) complex,  [Ni(HL1 )2](BF4)2*H2O, formed by coordination of two ligands with NNS donor atom sets (HL1 ligand, (E)-2-(1-(thiazol-2-yl)ethylidene)hydrazine-1-carbothioamide), and Ni(III) complex,  [Ni(L2 )(N3)3], formed by coordination of one hydrazone ligand coordinated through NNO donor  set and three azide anions (HL2Cl ligand, (E)−1-(2-oxo-2-(2-(1-(pyridin-2- yl)ethylidene)hydrazinyl)ethyl)pyridin-1-ium chloride), complemented by DFT calculations. To the best of our knowledge this is the first Ni(III) complex obtained under ambient  conditions.
PB  - Elsevier
T2  - Inorganic Chemistry Communications
T1  - Nickel(II) and nickel(III) Thiosemicarbazone and Hydrazone Complexes: An Unexpected Journey
VL  - 158
SP  - 111582
DO  - 10.1016/j.inoche.2023.111582
ER  - 
@article{
author = "Jevtović, Mima and Pevec, Andrej and Turel, Iztok and Radanović, Dušanka and Milčić, Miloš and Gruden, Maja and Zlatar, Matija and Mitić, Dragana and Anđelković, Katarina and Čobeljić, Božidar",
year = "2023",
abstract = "In this paper, we report the  synthesis, characterization, and X-ray structure analysis of one octahedral Ni(II) complex,  [Ni(HL1 )2](BF4)2*H2O, formed by coordination of two ligands with NNS donor atom sets (HL1 ligand, (E)-2-(1-(thiazol-2-yl)ethylidene)hydrazine-1-carbothioamide), and Ni(III) complex,  [Ni(L2 )(N3)3], formed by coordination of one hydrazone ligand coordinated through NNO donor  set and three azide anions (HL2Cl ligand, (E)−1-(2-oxo-2-(2-(1-(pyridin-2- yl)ethylidene)hydrazinyl)ethyl)pyridin-1-ium chloride), complemented by DFT calculations. To the best of our knowledge this is the first Ni(III) complex obtained under ambient  conditions.",
publisher = "Elsevier",
journal = "Inorganic Chemistry Communications",
title = "Nickel(II) and nickel(III) Thiosemicarbazone and Hydrazone Complexes: An Unexpected Journey",
volume = "158",
pages = "111582",
doi = "10.1016/j.inoche.2023.111582"
}
Jevtović, M., Pevec, A., Turel, I., Radanović, D., Milčić, M., Gruden, M., Zlatar, M., Mitić, D., Anđelković, K.,& Čobeljić, B.. (2023). Nickel(II) and nickel(III) Thiosemicarbazone and Hydrazone Complexes: An Unexpected Journey. in Inorganic Chemistry Communications
Elsevier., 158, 111582.
https://doi.org/10.1016/j.inoche.2023.111582
Jevtović M, Pevec A, Turel I, Radanović D, Milčić M, Gruden M, Zlatar M, Mitić D, Anđelković K, Čobeljić B. Nickel(II) and nickel(III) Thiosemicarbazone and Hydrazone Complexes: An Unexpected Journey. in Inorganic Chemistry Communications. 2023;158:111582.
doi:10.1016/j.inoche.2023.111582 .
Jevtović, Mima, Pevec, Andrej, Turel, Iztok, Radanović, Dušanka, Milčić, Miloš, Gruden, Maja, Zlatar, Matija, Mitić, Dragana, Anđelković, Katarina, Čobeljić, Božidar, "Nickel(II) and nickel(III) Thiosemicarbazone and Hydrazone Complexes: An Unexpected Journey" in Inorganic Chemistry Communications, 158 (2023):111582,
https://doi.org/10.1016/j.inoche.2023.111582 . .

The interplay between spin states, geometries and biological activity of Fe(III) and Mn(II) complexes with thiosemicarbazone

Stojičkov, Marko; Zlatar, Matija; Mazzeo, Paolo Pio; Bacchi, Alessia; Radanović, Dušanka D.; Stevanović, Nevena; Jevtović, Mima; Novaković, Irena T.; Anđelković, Katarina K.; Sladić, Dušan; Čobeljić, Božidar; Gruden, Maja

(Elsevier, 2023)

TY  - JOUR
AU  - Stojičkov, Marko
AU  - Zlatar, Matija
AU  - Mazzeo, Paolo Pio
AU  - Bacchi, Alessia
AU  - Radanović, Dušanka D.
AU  - Stevanović, Nevena
AU  - Jevtović, Mima
AU  - Novaković, Irena T.
AU  - Anđelković, Katarina K.
AU  - Sladić, Dušan
AU  - Čobeljić, Božidar
AU  - Gruden, Maja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6199
AB  - Fe(III) and Mn(II) complexes with condensation product of thiosemicarbazide and 2-acetylthiazole (HL1, (E)-2-(1-(thiazol-2-yl)ethylidene)hydrazine-1-carbothioamide) have been synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy, and elemental analysis. In both complexes, the thiosemicarbazone ligand is coordinated in deprotonated form through the NNS donor set of atoms. However, while Fe(III) complex is in the doublet ground state with distorted octahedral geometry, the coordination environment around Mn(II) is distorted trigonal-prismatic, and the sextet state is found to be the ground state. DFT calculations were performed to rationalize spin state preferences, and continuous shape measure describes the deviation from ideal six-coordinated polyhedral geometries in the ground and excited states. Antimicrobial activity (against a panel of Gram-negative and Gram-positive bacteria, two yeast, and one fungal strain), brine shrimp assay, and DPPH radical scavenging activity of both complexes were evaluated, and these results relate to the electronic structure of the complexes.
PB  - Elsevier
T2  - Polyhedron
T1  - The interplay between spin states, geometries and biological activity of Fe(III) and Mn(II) complexes with thiosemicarbazone
VL  - 237
SP  - 116389
DO  - 10.1016/j.poly.2023.116389
ER  - 
@article{
author = "Stojičkov, Marko and Zlatar, Matija and Mazzeo, Paolo Pio and Bacchi, Alessia and Radanović, Dušanka D. and Stevanović, Nevena and Jevtović, Mima and Novaković, Irena T. and Anđelković, Katarina K. and Sladić, Dušan and Čobeljić, Božidar and Gruden, Maja",
year = "2023",
abstract = "Fe(III) and Mn(II) complexes with condensation product of thiosemicarbazide and 2-acetylthiazole (HL1, (E)-2-(1-(thiazol-2-yl)ethylidene)hydrazine-1-carbothioamide) have been synthesized and characterized by single-crystal X-ray diffraction, IR spectroscopy, and elemental analysis. In both complexes, the thiosemicarbazone ligand is coordinated in deprotonated form through the NNS donor set of atoms. However, while Fe(III) complex is in the doublet ground state with distorted octahedral geometry, the coordination environment around Mn(II) is distorted trigonal-prismatic, and the sextet state is found to be the ground state. DFT calculations were performed to rationalize spin state preferences, and continuous shape measure describes the deviation from ideal six-coordinated polyhedral geometries in the ground and excited states. Antimicrobial activity (against a panel of Gram-negative and Gram-positive bacteria, two yeast, and one fungal strain), brine shrimp assay, and DPPH radical scavenging activity of both complexes were evaluated, and these results relate to the electronic structure of the complexes.",
publisher = "Elsevier",
journal = "Polyhedron",
title = "The interplay between spin states, geometries and biological activity of Fe(III) and Mn(II) complexes with thiosemicarbazone",
volume = "237",
pages = "116389",
doi = "10.1016/j.poly.2023.116389"
}
Stojičkov, M., Zlatar, M., Mazzeo, P. P., Bacchi, A., Radanović, D. D., Stevanović, N., Jevtović, M., Novaković, I. T., Anđelković, K. K., Sladić, D., Čobeljić, B.,& Gruden, M.. (2023). The interplay between spin states, geometries and biological activity of Fe(III) and Mn(II) complexes with thiosemicarbazone. in Polyhedron
Elsevier., 237, 116389.
https://doi.org/10.1016/j.poly.2023.116389
Stojičkov M, Zlatar M, Mazzeo PP, Bacchi A, Radanović DD, Stevanović N, Jevtović M, Novaković IT, Anđelković KK, Sladić D, Čobeljić B, Gruden M. The interplay between spin states, geometries and biological activity of Fe(III) and Mn(II) complexes with thiosemicarbazone. in Polyhedron. 2023;237:116389.
doi:10.1016/j.poly.2023.116389 .
Stojičkov, Marko, Zlatar, Matija, Mazzeo, Paolo Pio, Bacchi, Alessia, Radanović, Dušanka D., Stevanović, Nevena, Jevtović, Mima, Novaković, Irena T., Anđelković, Katarina K., Sladić, Dušan, Čobeljić, Božidar, Gruden, Maja, "The interplay between spin states, geometries and biological activity of Fe(III) and Mn(II) complexes with thiosemicarbazone" in Polyhedron, 237 (2023):116389,
https://doi.org/10.1016/j.poly.2023.116389 . .
1
1
1

Correlating Structure and KA2 Catalytic Activity of ZnII Hydrazone Complexes

Adejumo, Temiloluwa T.; Danopoulou, Marianna; Zorba, Leandros P.; Pevec, Andrej; Zlatar, Matija; Radanović, Dušanka D.; Savić, Milica; Gruden, Maja; Anđelković, Katarina K.; Turel, Iztok; Čobeljić, Božidar; Vougioukalakis, Georgios C.

(Wiley, 2023)

TY  - JOUR
AU  - Adejumo, Temiloluwa T.
AU  - Danopoulou, Marianna
AU  - Zorba, Leandros P.
AU  - Pevec, Andrej
AU  - Zlatar, Matija
AU  - Radanović, Dušanka D.
AU  - Savić, Milica
AU  - Gruden, Maja
AU  - Anđelković, Katarina K.
AU  - Turel, Iztok
AU  - Čobeljić, Božidar
AU  - Vougioukalakis, Georgios C.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6366
AB  - Two new Zn(II) complexes bearing tridentate hydrazone-based ligands with NNO or NNS donor atoms were synthesised and characterised by elemental analysis, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies, and single crystal X-ray diffraction methods. These complexes, together with four previously synthesised analogues, having hydrazone ligands with a NNO donor set of atoms, were successfully employed as catalysts in the ketone-amine-alkyne (KA2) coupling reaction, furnishing tetrasubstituted propargylamines, compounds with unique applications in organic chemistry. DFT calculations at the CAM-B3LYP/TZP level of theory were performed to elucidate the electronic structure of the investigated Zn(II) complexes, excellently correlating the structure of the complexes to their catalytic reactivity.
PB  - Wiley
T2  - European Journal of Inorganic Chemistry
T1  - Correlating Structure and KA2 Catalytic Activity of ZnII Hydrazone Complexes
VL  - 26
IS  - 33
SP  - e202300193
DO  - 10.1002/ejic.202300193
ER  - 
@article{
author = "Adejumo, Temiloluwa T. and Danopoulou, Marianna and Zorba, Leandros P. and Pevec, Andrej and Zlatar, Matija and Radanović, Dušanka D. and Savić, Milica and Gruden, Maja and Anđelković, Katarina K. and Turel, Iztok and Čobeljić, Božidar and Vougioukalakis, Georgios C.",
year = "2023",
abstract = "Two new Zn(II) complexes bearing tridentate hydrazone-based ligands with NNO or NNS donor atoms were synthesised and characterised by elemental analysis, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies, and single crystal X-ray diffraction methods. These complexes, together with four previously synthesised analogues, having hydrazone ligands with a NNO donor set of atoms, were successfully employed as catalysts in the ketone-amine-alkyne (KA2) coupling reaction, furnishing tetrasubstituted propargylamines, compounds with unique applications in organic chemistry. DFT calculations at the CAM-B3LYP/TZP level of theory were performed to elucidate the electronic structure of the investigated Zn(II) complexes, excellently correlating the structure of the complexes to their catalytic reactivity.",
publisher = "Wiley",
journal = "European Journal of Inorganic Chemistry",
title = "Correlating Structure and KA2 Catalytic Activity of ZnII Hydrazone Complexes",
volume = "26",
number = "33",
pages = "e202300193",
doi = "10.1002/ejic.202300193"
}
Adejumo, T. T., Danopoulou, M., Zorba, L. P., Pevec, A., Zlatar, M., Radanović, D. D., Savić, M., Gruden, M., Anđelković, K. K., Turel, I., Čobeljić, B.,& Vougioukalakis, G. C.. (2023). Correlating Structure and KA2 Catalytic Activity of ZnII Hydrazone Complexes. in European Journal of Inorganic Chemistry
Wiley., 26(33), e202300193.
https://doi.org/10.1002/ejic.202300193
Adejumo TT, Danopoulou M, Zorba LP, Pevec A, Zlatar M, Radanović DD, Savić M, Gruden M, Anđelković KK, Turel I, Čobeljić B, Vougioukalakis GC. Correlating Structure and KA2 Catalytic Activity of ZnII Hydrazone Complexes. in European Journal of Inorganic Chemistry. 2023;26(33):e202300193.
doi:10.1002/ejic.202300193 .
Adejumo, Temiloluwa T., Danopoulou, Marianna, Zorba, Leandros P., Pevec, Andrej, Zlatar, Matija, Radanović, Dušanka D., Savić, Milica, Gruden, Maja, Anđelković, Katarina K., Turel, Iztok, Čobeljić, Božidar, Vougioukalakis, Georgios C., "Correlating Structure and KA2 Catalytic Activity of ZnII Hydrazone Complexes" in European Journal of Inorganic Chemistry, 26, no. 33 (2023):e202300193,
https://doi.org/10.1002/ejic.202300193 . .
4
1

Dobijanje provodne elektrokeramike na bazi barijum-stanata dopiranog antimonom sa linearnom strujno-naponskom karakteristikom

Mitrović, Jelena

(Универзитет у Београду, Хемијски факултет, 2023)

TY  - THES
AU  - Mitrović, Jelena
PY  - 2023
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=9381
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:31851/bdef:Content/download
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/130953737
UR  - https://nardus.mpn.gov.rs/handle/123456789/21933
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6238
AB  - U okviru ove disertacije ispitivani su uticaji koncentracije dopanta i parametara sinterovanja nastrukturu i svojstva keramičkih uzoraka barijum-stanata dopiranog antimonom, BaSn1-xSbxO3(BSSO, x = 0,00; 0,04; 0,06; 0,08 i 0,10) u cilju dobijanja provodne elektrokeramike sa linearnomstrujno-naponskom (I-U) karakteristikom...
AB  - The main goal of this doctoral thesis was to investigate the influence of different dopantconcentrations and sintering parameters on the structure and properties of antimony-doped bariumstannate, BaSn1-xSbxO3 (BSSO, x = 0,00; 0,04; 0,06; 0,08 and 0,10) to obtain conductiveelectroceramic samples with linear current-voltage (I-U) characteristics...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Dobijanje provodne elektrokeramike na bazi barijum-stanata dopiranog antimonom sa linearnom strujno-naponskom karakteristikom
UR  - https://hdl.handle.net/21.15107/rcub_nardus_21933
ER  - 
@phdthesis{
author = "Mitrović, Jelena",
year = "2023",
abstract = "U okviru ove disertacije ispitivani su uticaji koncentracije dopanta i parametara sinterovanja nastrukturu i svojstva keramičkih uzoraka barijum-stanata dopiranog antimonom, BaSn1-xSbxO3(BSSO, x = 0,00; 0,04; 0,06; 0,08 i 0,10) u cilju dobijanja provodne elektrokeramike sa linearnomstrujno-naponskom (I-U) karakteristikom..., The main goal of this doctoral thesis was to investigate the influence of different dopantconcentrations and sintering parameters on the structure and properties of antimony-doped bariumstannate, BaSn1-xSbxO3 (BSSO, x = 0,00; 0,04; 0,06; 0,08 and 0,10) to obtain conductiveelectroceramic samples with linear current-voltage (I-U) characteristics...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Dobijanje provodne elektrokeramike na bazi barijum-stanata dopiranog antimonom sa linearnom strujno-naponskom karakteristikom",
url = "https://hdl.handle.net/21.15107/rcub_nardus_21933"
}
Mitrović, J.. (2023). Dobijanje provodne elektrokeramike na bazi barijum-stanata dopiranog antimonom sa linearnom strujno-naponskom karakteristikom. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_21933
Mitrović J. Dobijanje provodne elektrokeramike na bazi barijum-stanata dopiranog antimonom sa linearnom strujno-naponskom karakteristikom. in Универзитет у Београду. 2023;.
https://hdl.handle.net/21.15107/rcub_nardus_21933 .
Mitrović, Jelena, "Dobijanje provodne elektrokeramike na bazi barijum-stanata dopiranog antimonom sa linearnom strujno-naponskom karakteristikom" in Универзитет у Београду (2023),
https://hdl.handle.net/21.15107/rcub_nardus_21933 .

Određivanje mezotriona elektrodom od borom dopovanog dijamanta

Arbutina, Dušan

(2023)

TY  - THES
AU  - Arbutina, Dušan
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6113
AB  - Različite vrste pesticida se koriste za poboljšavanje poljoprivredne produktivnosti i 
održavanje svežine poljoprivrednih namirnica. Zbog sve veće i nekontrolisane primene,
pesticidi i njihovi derivati predstavljaju velike pretnje za ekosistem. Razvoj novih modernih 
metoda za njihovo praćenje i detekciju je od primarnog značaja. U ovom radu je korišćena 
nemodifikovana dijamantska elektroda dopovana borom. Cilj ovog rada je jednostavno, brzo, 
efikasno izvršiti detekciju mezotriona, opasnog herbicida koji se najviše koristi u kulturi 
kukuruza. Prvi put je korišćena nemodifikovana dijamantska elektroda dopovana borom za 
detekciju mezotriona. Struktura površine same elektrode omogućava uspešnu oksidaciju 
mezotriona pri viskoj vrednosti potencijala (+1.4V). Postignuta je granica detekcije 0,34μM i 
izuzetno dobra selektivnost između interferirajućih molekula. Ovom metodom omogućena je
laka i jednostavna primena u analizi realnih uzoraka – prehrambenih proizvoda kukuruznog 
porekla.
T1  - Određivanje mezotriona elektrodom od borom dopovanog dijamanta
SP  - 1
EP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6113
ER  - 
@mastersthesis{
author = "Arbutina, Dušan",
year = "2023",
abstract = "Različite vrste pesticida se koriste za poboljšavanje poljoprivredne produktivnosti i 
održavanje svežine poljoprivrednih namirnica. Zbog sve veće i nekontrolisane primene,
pesticidi i njihovi derivati predstavljaju velike pretnje za ekosistem. Razvoj novih modernih 
metoda za njihovo praćenje i detekciju je od primarnog značaja. U ovom radu je korišćena 
nemodifikovana dijamantska elektroda dopovana borom. Cilj ovog rada je jednostavno, brzo, 
efikasno izvršiti detekciju mezotriona, opasnog herbicida koji se najviše koristi u kulturi 
kukuruza. Prvi put je korišćena nemodifikovana dijamantska elektroda dopovana borom za 
detekciju mezotriona. Struktura površine same elektrode omogućava uspešnu oksidaciju 
mezotriona pri viskoj vrednosti potencijala (+1.4V). Postignuta je granica detekcije 0,34μM i 
izuzetno dobra selektivnost između interferirajućih molekula. Ovom metodom omogućena je
laka i jednostavna primena u analizi realnih uzoraka – prehrambenih proizvoda kukuruznog 
porekla.",
title = "Određivanje mezotriona elektrodom od borom dopovanog dijamanta",
pages = "1-23",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6113"
}
Arbutina, D.. (2023). Određivanje mezotriona elektrodom od borom dopovanog dijamanta. , 1-23.
https://hdl.handle.net/21.15107/rcub_cherry_6113
Arbutina D. Određivanje mezotriona elektrodom od borom dopovanog dijamanta. 2023;:1-23.
https://hdl.handle.net/21.15107/rcub_cherry_6113 .
Arbutina, Dušan, "Određivanje mezotriona elektrodom od borom dopovanog dijamanta" (2023):1-23,
https://hdl.handle.net/21.15107/rcub_cherry_6113 .

Interactions of Different Urolithins With Bovine Serum Albumin

Zelenović, Nevena D.; Kojadinović, Milica I.; Filipović, Lidija; Vučić, Vesna M.; Milčić, Miloš K.; Arsic, Aleksndra; Popović, Milica M.

(SAGE Publications, 2023)

TY  - JOUR
AU  - Zelenović, Nevena D.
AU  - Kojadinović, Milica I.
AU  - Filipović, Lidija
AU  - Vučić, Vesna M.
AU  - Milčić, Miloš K.
AU  - Arsic, Aleksndra
AU  - Popović, Milica M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6257
AB  - Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.
PB  - SAGE Publications
T2  - Natural Product Communications
T1  - Interactions of Different Urolithins With Bovine Serum Albumin
VL  - 18
IS  - 5
DO  - 10.1177/1934578X231169366
ER  - 
@article{
author = "Zelenović, Nevena D. and Kojadinović, Milica I. and Filipović, Lidija and Vučić, Vesna M. and Milčić, Miloš K. and Arsic, Aleksndra and Popović, Milica M.",
year = "2023",
abstract = "Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.",
publisher = "SAGE Publications",
journal = "Natural Product Communications",
title = "Interactions of Different Urolithins With Bovine Serum Albumin",
volume = "18",
number = "5",
doi = "10.1177/1934578X231169366"
}
Zelenović, N. D., Kojadinović, M. I., Filipović, L., Vučić, V. M., Milčić, M. K., Arsic, A.,& Popović, M. M.. (2023). Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications
SAGE Publications., 18(5).
https://doi.org/10.1177/1934578X231169366
Zelenović ND, Kojadinović MI, Filipović L, Vučić VM, Milčić MK, Arsic A, Popović MM. Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications. 2023;18(5).
doi:10.1177/1934578X231169366 .
Zelenović, Nevena D., Kojadinović, Milica I., Filipović, Lidija, Vučić, Vesna M., Milčić, Miloš K., Arsic, Aleksndra, Popović, Milica M., "Interactions of Different Urolithins With Bovine Serum Albumin" in Natural Product Communications, 18, no. 5 (2023),
https://doi.org/10.1177/1934578X231169366 . .

Sinteza i karakterizacija kompleksa Zn(II) sa kondenzacionim proizvodom 2-acetiltiazola i tiosemikarbazida

Razić, Maja

(2023)

TY  - THES
AU  - Razić, Maja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6132
AB  - U ovom radu je sintetisan i strukturnom analizom (infracrvenom spektroskopijom, 
nuklearno magnetnom rezonancijom, rendgenskom strukturnom analizom, elementalnom 
analizom) okarakterisan kompleks Zn(II) sa kondenzacionim proizvodom 2-acetiltiazola i 
tiosemikarbazida. Ligand koji je korišćen za ovu sintezu je HL1
-(E)-2-(1-(tiazol-2-
il)etiliden)hidrazin-1-karbotioamid.
T1  - Sinteza i karakterizacija kompleksa Zn(II) sa  kondenzacionim proizvodom 2-acetiltiazola i  tiosemikarbazida
SP  - 1
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6132
ER  - 
@mastersthesis{
author = "Razić, Maja",
year = "2023",
abstract = "U ovom radu je sintetisan i strukturnom analizom (infracrvenom spektroskopijom, 
nuklearno magnetnom rezonancijom, rendgenskom strukturnom analizom, elementalnom 
analizom) okarakterisan kompleks Zn(II) sa kondenzacionim proizvodom 2-acetiltiazola i 
tiosemikarbazida. Ligand koji je korišćen za ovu sintezu je HL1
-(E)-2-(1-(tiazol-2-
il)etiliden)hidrazin-1-karbotioamid.",
title = "Sinteza i karakterizacija kompleksa Zn(II) sa  kondenzacionim proizvodom 2-acetiltiazola i  tiosemikarbazida",
pages = "1-19",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6132"
}
Razić, M.. (2023). Sinteza i karakterizacija kompleksa Zn(II) sa  kondenzacionim proizvodom 2-acetiltiazola i  tiosemikarbazida. , 1-19.
https://hdl.handle.net/21.15107/rcub_cherry_6132
Razić M. Sinteza i karakterizacija kompleksa Zn(II) sa  kondenzacionim proizvodom 2-acetiltiazola i  tiosemikarbazida. 2023;:1-19.
https://hdl.handle.net/21.15107/rcub_cherry_6132 .
Razić, Maja, "Sinteza i karakterizacija kompleksa Zn(II) sa  kondenzacionim proizvodom 2-acetiltiazola i  tiosemikarbazida" (2023):1-19,
https://hdl.handle.net/21.15107/rcub_cherry_6132 .

On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae)

Vranić, Sofija; Vujisić, Ljubodrag V.; Vesović, Nikola; Todosijević, Marina; Pavićević, Miloš; Radović, Dejan; Ćurčić, Srećko

(MDPI, 2023)

TY  - JOUR
AU  - Vranić, Sofija
AU  - Vujisić, Ljubodrag V.
AU  - Vesović, Nikola
AU  - Todosijević, Marina
AU  - Pavićević, Miloš
AU  - Radović, Dejan
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5891
AB  - Pygidial glands are of great importance to ground beetles for defense against predators, especially for the species that live in subterranean habitats. The purpose of our study is to better understand the chemistry of the pygidial gland secretions of subterranean ground beetles, as well as the function and structure of the glands. We studied both the chemical composition of the pygidial gland secretion and morphology of the glands in adults of the troglophilic ground beetle species Laemostenus (Antisphodrus) cavicola (Schaum, 1858). The chemical composition of its defensive secretion was revealed using gas chromatography-mass spectrometry (GC-MS), while pygidial gland morphology of the beetle was investigated using bright-field microcopy. In total, seven chemical compounds were detected in the secretion mixture. Formic acid was the most dominant compound, followed by dodecyl acetate and undecane. Other chemicals were present in minor amounts. The morphological structure of the pygidial glands of L. (A.) cavicola was compared with the structure of the glands of the related congeneric troglophilic species Laemostenus (Pristonychus) punctatus (Dejean, 1828). Summary data on the semiochemicals that have been recorded so far in subterranean ground beetle species are presented, and the differences in the chemical composition of the secretions between and among troglobitic and troglophilic species are discussed. So far, forty-four compounds have been detected in four subterranean ground beetle species (two troglobites belonging to the tribe Trechini and two troglophiles belonging to the tribe Sphodrini). The results of this study indicate the great diversity of chemicals in the pygidial gland secretions of subterranean ground beetles. © 2023 by the authors.
PB  - MDPI
T2  - Diversity
T1  - On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae)
VL  - 15
IS  - 2
DO  - 10.3390/d15020136
ER  - 
@article{
author = "Vranić, Sofija and Vujisić, Ljubodrag V. and Vesović, Nikola and Todosijević, Marina and Pavićević, Miloš and Radović, Dejan and Ćurčić, Srećko",
year = "2023",
abstract = "Pygidial glands are of great importance to ground beetles for defense against predators, especially for the species that live in subterranean habitats. The purpose of our study is to better understand the chemistry of the pygidial gland secretions of subterranean ground beetles, as well as the function and structure of the glands. We studied both the chemical composition of the pygidial gland secretion and morphology of the glands in adults of the troglophilic ground beetle species Laemostenus (Antisphodrus) cavicola (Schaum, 1858). The chemical composition of its defensive secretion was revealed using gas chromatography-mass spectrometry (GC-MS), while pygidial gland morphology of the beetle was investigated using bright-field microcopy. In total, seven chemical compounds were detected in the secretion mixture. Formic acid was the most dominant compound, followed by dodecyl acetate and undecane. Other chemicals were present in minor amounts. The morphological structure of the pygidial glands of L. (A.) cavicola was compared with the structure of the glands of the related congeneric troglophilic species Laemostenus (Pristonychus) punctatus (Dejean, 1828). Summary data on the semiochemicals that have been recorded so far in subterranean ground beetle species are presented, and the differences in the chemical composition of the secretions between and among troglobitic and troglophilic species are discussed. So far, forty-four compounds have been detected in four subterranean ground beetle species (two troglobites belonging to the tribe Trechini and two troglophiles belonging to the tribe Sphodrini). The results of this study indicate the great diversity of chemicals in the pygidial gland secretions of subterranean ground beetles. © 2023 by the authors.",
publisher = "MDPI",
journal = "Diversity",
title = "On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae)",
volume = "15",
number = "2",
doi = "10.3390/d15020136"
}
Vranić, S., Vujisić, L. V., Vesović, N., Todosijević, M., Pavićević, M., Radović, D.,& Ćurčić, S.. (2023). On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae). in Diversity
MDPI., 15(2).
https://doi.org/10.3390/d15020136
Vranić S, Vujisić LV, Vesović N, Todosijević M, Pavićević M, Radović D, Ćurčić S. On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae). in Diversity. 2023;15(2).
doi:10.3390/d15020136 .
Vranić, Sofija, Vujisić, Ljubodrag V., Vesović, Nikola, Todosijević, Marina, Pavićević, Miloš, Radović, Dejan, Ćurčić, Srećko, "On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae)" in Diversity, 15, no. 2 (2023),
https://doi.org/10.3390/d15020136 . .
2

The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)

Vesović, Nikola; Nenadić, Marija; Vranić, Sofija; Vujisić, Ljubodrag V.; Milinčić, Katarina M.; Todosijević, Marina; Dimkić, Ivica; Janakiev, Tamara; Ćurčić, Nina B.; Stevanović, Nataša; Mihajlović, Ljiljana; Vukoičić, Danijela Ž.; Ćurčić, Srećko

(Frontiers Media, 2023)

TY  - JOUR
AU  - Vesović, Nikola
AU  - Nenadić, Marija
AU  - Vranić, Sofija
AU  - Vujisić, Ljubodrag V.
AU  - Milinčić, Katarina M.
AU  - Todosijević, Marina
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Ćurčić, Nina B.
AU  - Stevanović, Nataša
AU  - Mihajlović, Ljiljana
AU  - Vukoičić, Danijela Ž.
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6189
AB  - The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.
PB  - Frontiers Media
T2  - Frontiers in Ecology and Evolution
T1  - The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)
VL  - 11
DO  - 10.3389/fevo.2023.1120006
ER  - 
@article{
author = "Vesović, Nikola and Nenadić, Marija and Vranić, Sofija and Vujisić, Ljubodrag V. and Milinčić, Katarina M. and Todosijević, Marina and Dimkić, Ivica and Janakiev, Tamara and Ćurčić, Nina B. and Stevanović, Nataša and Mihajlović, Ljiljana and Vukoičić, Danijela Ž. and Ćurčić, Srećko",
year = "2023",
abstract = "The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.",
publisher = "Frontiers Media",
journal = "Frontiers in Ecology and Evolution",
title = "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)",
volume = "11",
doi = "10.3389/fevo.2023.1120006"
}
Vesović, N., Nenadić, M., Vranić, S., Vujisić, L. V., Milinčić, K. M., Todosijević, M., Dimkić, I., Janakiev, T., Ćurčić, N. B., Stevanović, N., Mihajlović, L., Vukoičić, D. Ž.,& Ćurčić, S.. (2023). The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution
Frontiers Media., 11.
https://doi.org/10.3389/fevo.2023.1120006
Vesović N, Nenadić M, Vranić S, Vujisić LV, Milinčić KM, Todosijević M, Dimkić I, Janakiev T, Ćurčić NB, Stevanović N, Mihajlović L, Vukoičić DŽ, Ćurčić S. The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution. 2023;11.
doi:10.3389/fevo.2023.1120006 .
Vesović, Nikola, Nenadić, Marija, Vranić, Sofija, Vujisić, Ljubodrag V., Milinčić, Katarina M., Todosijević, Marina, Dimkić, Ivica, Janakiev, Tamara, Ćurčić, Nina B., Stevanović, Nataša, Mihajlović, Ljiljana, Vukoičić, Danijela Ž., Ćurčić, Srećko, "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)" in Frontiers in Ecology and Evolution, 11 (2023),
https://doi.org/10.3389/fevo.2023.1120006 . .
1
1
1

The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.

Nenadić, Marija; Stojković, Dejan; Soković, Marina; Ćirić, Ana; Dimkić, Ivica; Janakiev, Tamara; Vesović, Nikola; Vujisić, Ljubodrag V.; Todosijević, Marina; Stanković, Saša S; Ćurčić, Nina B.; Milinčić, Uroš; Petrović, Dragan; Milinčić, Miroljub; Ćurčić, Srećko

(Frontiers Media S.A., 2023)

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag V.
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6270
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
SP  - 1148309
DO  - 10.3389/fevo.2023.1148309
ER  - 
@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag V. and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
pages = "1148309",
doi = "10.3389/fevo.2023.1148309"
}
Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L. V., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309
Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić LV, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .
Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag V., Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .
1

SERBIAN BLACK TRUFFLE TUBER AESTIVUM: MICROBIOTA AND EFFECTS OF DIFFERENT FREEZING REGIMES ON VOLATILE AROMA COMPOUNDS DURING STORAGE

Paunović, Dušanka D.; Mirković, Milica M.; Mirković, Nemanja L.; Tešević, Vele V.; Stanković Jeremić, Jovana M.; Todosijević, Marina M.; Radulović, Zorica T.

(2023)

TY  - JOUR
AU  - Paunović, Dušanka D.
AU  - Mirković, Milica M.
AU  - Mirković, Nemanja L.
AU  - Tešević, Vele V.
AU  - Stanković Jeremić, Jovana M.
AU  - Todosijević, Marina M.
AU  - Radulović, Zorica T.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6379
AB  - The use of truffles in food is based mainly on the addition of artificial flavors, aiming to achieve an intense aroma in the products. As truffle is a natural product with nutritional and functional properties, it is important to find an optimal method for truffle storage. As the microbiota contribute to truffle aroma, the bacterial and yeast compositions in the rhizosphere and fruiting body of the truffle and the impact of different freezing methods on the volatile profile of the truffle Tuber aestivum during 90 days of the storage were determined. Bacteria and yeasts isolates were identified using 16s rRNA and 18s rRNA. The effect of freezing truffles at -20°C and -80°C with and without previous dipping in liquid N2 on the volatile compounds was observed using GC/MS. The results demonstrated that the isolated bacteria belonged to the phylum Proteobacteria, Firmicutes and Actinobacteria, and the identified species mainly belonged to Firmicutes, genus Bacillus sp. Isolated yeasts were identified as Cryptococcus sp., Debaromyces hanseinii, Candida fermentati and Rhodotorula mucilaginosa. The GC/MS analysis revealed that frozen truffle samples were richer in the compounds 2-butanone, 2-methyl-butanal, methanethiol and 2-butanol after freezing or during storage. The content of DMS, acetaldehyde, 3-octanone, ethanol, and 2-methyl-1-propanol significantly decreased immediately after freezing. Overall, the gained results indicated that freezing of truffles as a preservation method had profound effects on the volatile compounds, while previous dipping in liquid N2 showed no significant impact on the volatile profile of truffle Tuber aestivum. © 2023 University of Belgrade - Faculty of Agriculture. All Rights Reserved.
T2  - Journal of Agricultural Sciences (Belgrade)
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - SERBIAN BLACK TRUFFLE TUBER AESTIVUM: MICROBIOTA AND EFFECTS OF DIFFERENT FREEZING REGIMES ON VOLATILE AROMA COMPOUNDS DURING STORAGE
VL  - 68
IS  - 3
SP  - 329
EP  - 346
DO  - 10.2298/JAS2303329P
ER  - 
@article{
author = "Paunović, Dušanka D. and Mirković, Milica M. and Mirković, Nemanja L. and Tešević, Vele V. and Stanković Jeremić, Jovana M. and Todosijević, Marina M. and Radulović, Zorica T.",
year = "2023",
abstract = "The use of truffles in food is based mainly on the addition of artificial flavors, aiming to achieve an intense aroma in the products. As truffle is a natural product with nutritional and functional properties, it is important to find an optimal method for truffle storage. As the microbiota contribute to truffle aroma, the bacterial and yeast compositions in the rhizosphere and fruiting body of the truffle and the impact of different freezing methods on the volatile profile of the truffle Tuber aestivum during 90 days of the storage were determined. Bacteria and yeasts isolates were identified using 16s rRNA and 18s rRNA. The effect of freezing truffles at -20°C and -80°C with and without previous dipping in liquid N2 on the volatile compounds was observed using GC/MS. The results demonstrated that the isolated bacteria belonged to the phylum Proteobacteria, Firmicutes and Actinobacteria, and the identified species mainly belonged to Firmicutes, genus Bacillus sp. Isolated yeasts were identified as Cryptococcus sp., Debaromyces hanseinii, Candida fermentati and Rhodotorula mucilaginosa. The GC/MS analysis revealed that frozen truffle samples were richer in the compounds 2-butanone, 2-methyl-butanal, methanethiol and 2-butanol after freezing or during storage. The content of DMS, acetaldehyde, 3-octanone, ethanol, and 2-methyl-1-propanol significantly decreased immediately after freezing. Overall, the gained results indicated that freezing of truffles as a preservation method had profound effects on the volatile compounds, while previous dipping in liquid N2 showed no significant impact on the volatile profile of truffle Tuber aestivum. © 2023 University of Belgrade - Faculty of Agriculture. All Rights Reserved.",
journal = "Journal of Agricultural Sciences (Belgrade), Journal of Agricultural Sciences (Belgrade)",
title = "SERBIAN BLACK TRUFFLE TUBER AESTIVUM: MICROBIOTA AND EFFECTS OF DIFFERENT FREEZING REGIMES ON VOLATILE AROMA COMPOUNDS DURING STORAGE",
volume = "68",
number = "3",
pages = "329-346",
doi = "10.2298/JAS2303329P"
}
Paunović, D. D., Mirković, M. M., Mirković, N. L., Tešević, V. V., Stanković Jeremić, J. M., Todosijević, M. M.,& Radulović, Z. T.. (2023). SERBIAN BLACK TRUFFLE TUBER AESTIVUM: MICROBIOTA AND EFFECTS OF DIFFERENT FREEZING REGIMES ON VOLATILE AROMA COMPOUNDS DURING STORAGE. in Journal of Agricultural Sciences (Belgrade), 68(3), 329-346.
https://doi.org/10.2298/JAS2303329P
Paunović DD, Mirković MM, Mirković NL, Tešević VV, Stanković Jeremić JM, Todosijević MM, Radulović ZT. SERBIAN BLACK TRUFFLE TUBER AESTIVUM: MICROBIOTA AND EFFECTS OF DIFFERENT FREEZING REGIMES ON VOLATILE AROMA COMPOUNDS DURING STORAGE. in Journal of Agricultural Sciences (Belgrade). 2023;68(3):329-346.
doi:10.2298/JAS2303329P .
Paunović, Dušanka D., Mirković, Milica M., Mirković, Nemanja L., Tešević, Vele V., Stanković Jeremić, Jovana M., Todosijević, Marina M., Radulović, Zorica T., "SERBIAN BLACK TRUFFLE TUBER AESTIVUM: MICROBIOTA AND EFFECTS OF DIFFERENT FREEZING REGIMES ON VOLATILE AROMA COMPOUNDS DURING STORAGE" in Journal of Agricultural Sciences (Belgrade), 68, no. 3 (2023):329-346,
https://doi.org/10.2298/JAS2303329P . .

Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency

Veljović Jovanović, Sonja; Kasalica, Bećko; Miletić, Katarina; Vidović, Marija; Šušić, Nikola; Jeremić, Dejan; Belča, Ivan

(MDPI, 2023)

TY  - JOUR
AU  - Veljović Jovanović, Sonja
AU  - Kasalica, Bećko
AU  - Miletić, Katarina
AU  - Vidović, Marija
AU  - Šušić, Nikola
AU  - Jeremić, Dejan
AU  - Belča, Ivan
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6380
AB  - Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency
VL  - 24
IS  - 18
SP  - 14265
DO  - 10.3390/ijms241814265
ER  - 
@article{
author = "Veljović Jovanović, Sonja and Kasalica, Bećko and Miletić, Katarina and Vidović, Marija and Šušić, Nikola and Jeremić, Dejan and Belča, Ivan",
year = "2023",
abstract = "Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency",
volume = "24",
number = "18",
pages = "14265",
doi = "10.3390/ijms241814265"
}
Veljović Jovanović, S., Kasalica, B., Miletić, K., Vidović, M., Šušić, N., Jeremić, D.,& Belča, I.. (2023). Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences
MDPI., 24(18), 14265.
https://doi.org/10.3390/ijms241814265
Veljović Jovanović S, Kasalica B, Miletić K, Vidović M, Šušić N, Jeremić D, Belča I. Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences. 2023;24(18):14265.
doi:10.3390/ijms241814265 .
Veljović Jovanović, Sonja, Kasalica, Bećko, Miletić, Katarina, Vidović, Marija, Šušić, Nikola, Jeremić, Dejan, Belča, Ivan, "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency" in International Journal of Molecular Sciences, 24, no. 18 (2023):14265,
https://doi.org/10.3390/ijms241814265 . .

Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia

Sofrenić, Ivana; Anđelković, Boban D.; Gođevac, Dejan; Ivanović, Stefan; Simić, Katarina; Ljujić, Jovana; Tešević, Vele; Milosavljević, Slobodan M.

(MDPI, 2023)

TY  - JOUR
AU  - Sofrenić, Ivana
AU  - Anđelković, Boban D.
AU  - Gođevac, Dejan
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Ljujić, Jovana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5811
AB  - Chemotaxonomy presents various challenges that need to be overcome in order to obtain valid and reliable results. Individual genetic and environmental variations can give a false picture and lead to wrong conclusions. Applying a holistic approach, based on multivariate data analysis, these challenges can be overcome. Thus, a metabolomics approach has to be optimized depending on the subject of research. We used 1H NMR-based metabolomics as a potential chemotaxonomic tool on the selected Euphorbia species growing wild in Serbia. Principal components analysis (PCA), soft independent modeling by class analogy (SIMCA) and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) were used to analyze obtained NMR data in order to reveal chemotaxonomic biomarkers. The standard protocol for plant metabolomics was optimized aiming to extract more specific metabolites, which are characteristic for the Euphorbia genus. The obtained models were validated, which revealed that variables unique for each species were associated with certain classes of molecules according to literature data. In E. salicifolia, acacetin-7-O-glycoside (not found before in the species) was detected, and the structure of the aglycone part was solved based on 2D NMR data. In the presented paper, we have shown that metabolomics can be successfully used in Euphorbia chemotaxonomy.
PB  - MDPI
T2  - Plants
T1  - Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia
VL  - 12
IS  - 2
SP  - 262
DO  - 10.3390/plants12020262
ER  - 
@article{
author = "Sofrenić, Ivana and Anđelković, Boban D. and Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Ljujić, Jovana and Tešević, Vele and Milosavljević, Slobodan M.",
year = "2023",
abstract = "Chemotaxonomy presents various challenges that need to be overcome in order to obtain valid and reliable results. Individual genetic and environmental variations can give a false picture and lead to wrong conclusions. Applying a holistic approach, based on multivariate data analysis, these challenges can be overcome. Thus, a metabolomics approach has to be optimized depending on the subject of research. We used 1H NMR-based metabolomics as a potential chemotaxonomic tool on the selected Euphorbia species growing wild in Serbia. Principal components analysis (PCA), soft independent modeling by class analogy (SIMCA) and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) were used to analyze obtained NMR data in order to reveal chemotaxonomic biomarkers. The standard protocol for plant metabolomics was optimized aiming to extract more specific metabolites, which are characteristic for the Euphorbia genus. The obtained models were validated, which revealed that variables unique for each species were associated with certain classes of molecules according to literature data. In E. salicifolia, acacetin-7-O-glycoside (not found before in the species) was detected, and the structure of the aglycone part was solved based on 2D NMR data. In the presented paper, we have shown that metabolomics can be successfully used in Euphorbia chemotaxonomy.",
publisher = "MDPI",
journal = "Plants",
title = "Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia",
volume = "12",
number = "2",
pages = "262",
doi = "10.3390/plants12020262"
}
Sofrenić, I., Anđelković, B. D., Gođevac, D., Ivanović, S., Simić, K., Ljujić, J., Tešević, V.,& Milosavljević, S. M.. (2023). Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia. in Plants
MDPI., 12(2), 262.
https://doi.org/10.3390/plants12020262
Sofrenić I, Anđelković BD, Gođevac D, Ivanović S, Simić K, Ljujić J, Tešević V, Milosavljević SM. Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia. in Plants. 2023;12(2):262.
doi:10.3390/plants12020262 .
Sofrenić, Ivana, Anđelković, Boban D., Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Ljujić, Jovana, Tešević, Vele, Milosavljević, Slobodan M., "Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia" in Plants, 12, no. 2 (2023):262,
https://doi.org/10.3390/plants12020262 . .
1

The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstač and Žižak: Scientific paper

Madžgalj, Valerija; Petrović, Aleksandar; Čakar, Uroš; Maraš, Vesna; Sofrenić, Ivana; Tešević, Vele

(Belgrade : Serbian Chemical Society, 2023)

TY  - JOUR
AU  - Madžgalj, Valerija
AU  - Petrović, Aleksandar
AU  - Čakar, Uroš
AU  - Maraš, Vesna
AU  - Sofrenić, Ivana
AU  - Tešević, Vele
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5818
AB  - This study aimed to show aromatic profile of wines produced from two autochthonous grape cultivars Krstač (K) and Žižak (Z). During the wine production two enzymatic preparations (EP) Lallzyme cuvee blanc (CB) and Lallzyme enzymatic preparation β (EB) and different time of skin contact (4 and 8 h) were applied. Aromatic compounds were detected by GC/FID–MS ana­lysis. Significantly higher content of total detected aromatic compounds com­pared to appropriate controls (168.54 and 161.72 mg L-1) was observed for K EB4h (176.33 mg L-1) and Z CB4h (177.29 mg L-1) wines. Skin contact and usage of EP mostly increased content of 2-phenylethyl and isoamyl alcohols. Wines from both varieties showed higher content of hexanoic and octanoic acids compared to the control. It is interesting to emphasize that content of esters that are responsible for fruity aroma of wine which is important for plea­sant taste (isoamyl acetate – banana, ethyl hexanoate – ripe banana, 2-phe­nyl­ethyl acetate – powerful fruity rose like) were increased in all samples com­pared to the controls. The highest grades, after sensory evaluation, were obtained for K EB 8h (18.0 out of 20.0) and Z CB 8h (18.2 out of 20.0).
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstač and Žižak: Scientific paper
VL  - 88
IS  - 1
SP  - 11
EP  - 23
EP  - 
DO  - 10.2298/JSC220311056M
ER  - 
@article{
author = "Madžgalj, Valerija and Petrović, Aleksandar and Čakar, Uroš and Maraš, Vesna and Sofrenić, Ivana and Tešević, Vele",
year = "2023",
abstract = "This study aimed to show aromatic profile of wines produced from two autochthonous grape cultivars Krstač (K) and Žižak (Z). During the wine production two enzymatic preparations (EP) Lallzyme cuvee blanc (CB) and Lallzyme enzymatic preparation β (EB) and different time of skin contact (4 and 8 h) were applied. Aromatic compounds were detected by GC/FID–MS ana­lysis. Significantly higher content of total detected aromatic compounds com­pared to appropriate controls (168.54 and 161.72 mg L-1) was observed for K EB4h (176.33 mg L-1) and Z CB4h (177.29 mg L-1) wines. Skin contact and usage of EP mostly increased content of 2-phenylethyl and isoamyl alcohols. Wines from both varieties showed higher content of hexanoic and octanoic acids compared to the control. It is interesting to emphasize that content of esters that are responsible for fruity aroma of wine which is important for plea­sant taste (isoamyl acetate – banana, ethyl hexanoate – ripe banana, 2-phe­nyl­ethyl acetate – powerful fruity rose like) were increased in all samples com­pared to the controls. The highest grades, after sensory evaluation, were obtained for K EB 8h (18.0 out of 20.0) and Z CB 8h (18.2 out of 20.0).",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstač and Žižak: Scientific paper",
volume = "88",
number = "1",
pages = "11-23-",
doi = "10.2298/JSC220311056M"
}
Madžgalj, V., Petrović, A., Čakar, U., Maraš, V., Sofrenić, I.,& Tešević, V.. (2023). The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstač and Žižak: Scientific paper. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 88(1), 11-23.
https://doi.org/10.2298/JSC220311056M
Madžgalj V, Petrović A, Čakar U, Maraš V, Sofrenić I, Tešević V. The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstač and Žižak: Scientific paper. in Journal of the Serbian Chemical Society. 2023;88(1):11-23.
doi:10.2298/JSC220311056M .
Madžgalj, Valerija, Petrović, Aleksandar, Čakar, Uroš, Maraš, Vesna, Sofrenić, Ivana, Tešević, Vele, "The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstač and Žižak: Scientific paper" in Journal of the Serbian Chemical Society, 88, no. 1 (2023):11-23,
https://doi.org/10.2298/JSC220311056M . .
8
7

Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts

Smiljanić, Katarina; Prodić, Ivana; Trifunović, Sara; Krstić-Ristivojević, Maja; Aćimović, Milica G.; Stanković Jeremić, Jovana; Lončar, Biljana; Tešević, Vele

(MDPI, 2023)

TY  - JOUR
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Trifunović, Sara
AU  - Krstić-Ristivojević, Maja
AU  - Aćimović, Milica G.
AU  - Stanković Jeremić, Jovana
AU  - Lončar, Biljana
AU  - Tešević, Vele
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6203
AB  - As byproducts of essential oil distillation, hydrolates are used in natural cosmetics/biomedicine due to their beneficial skin effects. However, data on their safety with relevant biological targets, such as human skin cells, are scarce. Therefore, we have tested nine hydrolates from the Lamiaceae family with skin fibroblasts that are responsible for extracellular collagenous matrix builds. Thyme, oregano, and winter savoury hydrolates showed several times higher total phenolics, which correlated strongly with their radical scavenging and antioxidative capacity; there was no correlation between their viability profiles and the reducing sugar levels. No proteins/peptides were detected. All hydrolates appeared safe for prolonged skin exposure except for 10-fold diluted lavender, which showed cytotoxicity (~20%), as well as rosemary and lavandin (~10%) using viability, DNA synthesis, and cell count testing. Clary sage, oregano, lemon balm, and thyme hydrolates (10-fold diluted) increased fibroblast viability and/or proliferation by 10–30% compared with the control, while their viability remained unaffected by Mentha and winter savoury. In line with the STITCH database, increased viability could be attributed to thymol presence in oregano and thyme hydrolates in lemon balm, which is most likely attributable to neral and geranial. The proliferative effect of clary sage could be supported by alpha-terpineol, not linalool. The major volatile organic compounds (VOCs) associated with cytotoxic effects on fibroblasts were borneol, 1,8-cineole, and terpinene-4-ol. Further research with pure compounds is warranted to confirm the roles of VOCs in the observed effects that are relevant to cosmetic and wound healing aspects.
PB  - MDPI
T2  - Antioxidants
T1  - Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts
VL  - 12
IS  - 11
DO  - 10.3390/antiox12111988
ER  - 
@article{
author = "Smiljanić, Katarina and Prodić, Ivana and Trifunović, Sara and Krstić-Ristivojević, Maja and Aćimović, Milica G. and Stanković Jeremić, Jovana and Lončar, Biljana and Tešević, Vele",
year = "2023",
abstract = "As byproducts of essential oil distillation, hydrolates are used in natural cosmetics/biomedicine due to their beneficial skin effects. However, data on their safety with relevant biological targets, such as human skin cells, are scarce. Therefore, we have tested nine hydrolates from the Lamiaceae family with skin fibroblasts that are responsible for extracellular collagenous matrix builds. Thyme, oregano, and winter savoury hydrolates showed several times higher total phenolics, which correlated strongly with their radical scavenging and antioxidative capacity; there was no correlation between their viability profiles and the reducing sugar levels. No proteins/peptides were detected. All hydrolates appeared safe for prolonged skin exposure except for 10-fold diluted lavender, which showed cytotoxicity (~20%), as well as rosemary and lavandin (~10%) using viability, DNA synthesis, and cell count testing. Clary sage, oregano, lemon balm, and thyme hydrolates (10-fold diluted) increased fibroblast viability and/or proliferation by 10–30% compared with the control, while their viability remained unaffected by Mentha and winter savoury. In line with the STITCH database, increased viability could be attributed to thymol presence in oregano and thyme hydrolates in lemon balm, which is most likely attributable to neral and geranial. The proliferative effect of clary sage could be supported by alpha-terpineol, not linalool. The major volatile organic compounds (VOCs) associated with cytotoxic effects on fibroblasts were borneol, 1,8-cineole, and terpinene-4-ol. Further research with pure compounds is warranted to confirm the roles of VOCs in the observed effects that are relevant to cosmetic and wound healing aspects.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts",
volume = "12",
number = "11",
doi = "10.3390/antiox12111988"
}
Smiljanić, K., Prodić, I., Trifunović, S., Krstić-Ristivojević, M., Aćimović, M. G., Stanković Jeremić, J., Lončar, B.,& Tešević, V.. (2023). Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts. in Antioxidants
MDPI., 12(11).
https://doi.org/10.3390/antiox12111988
Smiljanić K, Prodić I, Trifunović S, Krstić-Ristivojević M, Aćimović MG, Stanković Jeremić J, Lončar B, Tešević V. Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts. in Antioxidants. 2023;12(11).
doi:10.3390/antiox12111988 .
Smiljanić, Katarina, Prodić, Ivana, Trifunović, Sara, Krstić-Ristivojević, Maja, Aćimović, Milica G., Stanković Jeremić, Jovana, Lončar, Biljana, Tešević, Vele, "Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts" in Antioxidants, 12, no. 11 (2023),
https://doi.org/10.3390/antiox12111988 . .

Izolovanje i određivanje strukture triterpenskog alkohola iz medicinske gljive Ganoderma resinaceum

Paunović, Jelena

(2023)

TY  - THES
AU  - Paunović, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6082
AB  - Predmet proučavanja ovog završnog rada je medicinska gljiva Ganoderma resinaceum poznata kao smolasta sjajnica. Osnovni cilj ovog završnog rada je bio izolovanje bioaktivnog jedinjenja triterpenskog tipa i određivanje strukture primenom 1D i 2D NMR spektroskopije.
U okviru ovog završnog rada je izolovano i okarakterisano jedinjenje triterpenskog tipa, ganoderiol B, koje je prvi put izolovano iz plodonosnih tela Ganoderma resinaceum.
T1  - Izolovanje i određivanje strukture triterpenskog alkohola iz medicinske gljive Ganoderma resinaceum
SP  - 1
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6082
ER  - 
@misc{
author = "Paunović, Jelena",
year = "2023",
abstract = "Predmet proučavanja ovog završnog rada je medicinska gljiva Ganoderma resinaceum poznata kao smolasta sjajnica. Osnovni cilj ovog završnog rada je bio izolovanje bioaktivnog jedinjenja triterpenskog tipa i određivanje strukture primenom 1D i 2D NMR spektroskopije.
U okviru ovog završnog rada je izolovano i okarakterisano jedinjenje triterpenskog tipa, ganoderiol B, koje je prvi put izolovano iz plodonosnih tela Ganoderma resinaceum.",
title = "Izolovanje i određivanje strukture triterpenskog alkohola iz medicinske gljive Ganoderma resinaceum",
pages = "1-30",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6082"
}
Paunović, J.. (2023). Izolovanje i određivanje strukture triterpenskog alkohola iz medicinske gljive Ganoderma resinaceum. , 1-30.
https://hdl.handle.net/21.15107/rcub_cherry_6082
Paunović J. Izolovanje i određivanje strukture triterpenskog alkohola iz medicinske gljive Ganoderma resinaceum. 2023;:1-30.
https://hdl.handle.net/21.15107/rcub_cherry_6082 .
Paunović, Jelena, "Izolovanje i određivanje strukture triterpenskog alkohola iz medicinske gljive Ganoderma resinaceum" (2023):1-30,
https://hdl.handle.net/21.15107/rcub_cherry_6082 .

Izolovanje i određivanje strukture flavonoida iz biljne vrste Cotinus coggygria

Jovković, Marija

(2023)

TY  - THES
AU  - Jovković, Marija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6134
AB  - C. coggygria, ruj ili “dimno drvo” je jedna od najpoznatijih vrsta biljnog roda Cotinus koja 
pripada velikoj familiji Anacardiaceae. Zbog raznovrsnosti metabolita koju ova biljna vrsta 
sintetiše i njihove biološke aktivnosti, česta je njena upotreba u tradicionalnoj medicini. Između 
ostalih, ističu se antiinflamatorna, antimikrobna i antikancerogena svojstva. Cilj ovog istraživanja 
bio je izolovanje i karakterizacija flavonoida iz biljne vrste C. coggygria. Primenom klasičnih i 
instrumentalnih hromatografskih metoda iz ekstrakata srži stabla ruja, izolovano je sedam 
jedinjenja: sulfuretin, fustin, fisetin, taksifolin, butin, butein i jedno novo jedinjenje 2,7,3’,4’-
tetrahidroksiflavanon. Struktura ovih jedinjenja određena je primenom 1D i 2D NMR 
spektroskopkih metoda.
T1  - Izolovanje i određivanje strukture flavonoida iz biljne  vrste Cotinus coggygria
SP  - 1
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6134
ER  - 
@mastersthesis{
author = "Jovković, Marija",
year = "2023",
abstract = "C. coggygria, ruj ili “dimno drvo” je jedna od najpoznatijih vrsta biljnog roda Cotinus koja 
pripada velikoj familiji Anacardiaceae. Zbog raznovrsnosti metabolita koju ova biljna vrsta 
sintetiše i njihove biološke aktivnosti, česta je njena upotreba u tradicionalnoj medicini. Između 
ostalih, ističu se antiinflamatorna, antimikrobna i antikancerogena svojstva. Cilj ovog istraživanja 
bio je izolovanje i karakterizacija flavonoida iz biljne vrste C. coggygria. Primenom klasičnih i 
instrumentalnih hromatografskih metoda iz ekstrakata srži stabla ruja, izolovano je sedam 
jedinjenja: sulfuretin, fustin, fisetin, taksifolin, butin, butein i jedno novo jedinjenje 2,7,3’,4’-
tetrahidroksiflavanon. Struktura ovih jedinjenja određena je primenom 1D i 2D NMR 
spektroskopkih metoda.",
title = "Izolovanje i određivanje strukture flavonoida iz biljne  vrste Cotinus coggygria",
pages = "1-63",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6134"
}
Jovković, M.. (2023). Izolovanje i određivanje strukture flavonoida iz biljne  vrste Cotinus coggygria. , 1-63.
https://hdl.handle.net/21.15107/rcub_cherry_6134
Jovković M. Izolovanje i određivanje strukture flavonoida iz biljne  vrste Cotinus coggygria. 2023;:1-63.
https://hdl.handle.net/21.15107/rcub_cherry_6134 .
Jovković, Marija, "Izolovanje i određivanje strukture flavonoida iz biljne  vrste Cotinus coggygria" (2023):1-63,
https://hdl.handle.net/21.15107/rcub_cherry_6134 .

Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens

Veljović, Katarina; Tešević, Vele; Mitrović, Hristina; Stanković, Marija

(Elsevier, 2023)

TY  - JOUR
AU  - Veljović, Katarina
AU  - Tešević, Vele
AU  - Mitrović, Hristina
AU  - Stanković, Marija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6190
AB  - IntroductionOregano essential oil (OEO) is one of the most widely used essential oils worldwide due to its huge therapeutic benefits. Nevertheless, data on the effects of the endemic species Origanum minutiflorum, also known as wild or Turkish oregano, is scarce. On the other hand, various chronic lung diseases, characterised by persistent inflammation, oxidative stress, and common bacterial infections, do not have effective pharmacological therapy. Hence, the aim of this study is to examine the effects of wild oregano essential oil (WOEO) on human bronchial epithelial cells and lung pathogens.MethodsWe provided a detailed chemical composition of WOEO using GC-MS and GC-FID analysis. Anti-inflammatory effects of WOEO were analysed using the qRT-PCR and ELISA methods, while antioxidative properties were examined by using the dichlorofluorescein assay in BEAS-2B cells. Antibacterial activity was tested on lung pathogens by using the agar diffusion assay.ResultsThe major constituents of WOEO, analysed in this study, were carvacrol, linalool, p-cymene, γ-terpinene, and (E)-caryophyllene. We found that treatment with WOEO attenuated LPS-induced IL8 gene expression and hydrogen peroxide-induced oxidative stress in BEAS-2B cells. Moreover, WOEO showed an inhibitory effect on pathogenic bacteria Acinetobacter baumannii and highly resistant Klebsiella pneumoniae commonly seen in healthcare-associated pneumonia.ConclusionOur work presents new insights into the anti-inflammatory, antioxidative, and antimicrobial properties of WOEO which may be used as a simple and local treatment in various chronic lung diseases.
PB  - Elsevier
T2  - Journal of Herbal Medicine
T1  - Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens
VL  - 39
SP  - 100651
DO  - 10.1016/j.hermed.2023.100651
ER  - 
@article{
author = "Veljović, Katarina and Tešević, Vele and Mitrović, Hristina and Stanković, Marija",
year = "2023",
abstract = "IntroductionOregano essential oil (OEO) is one of the most widely used essential oils worldwide due to its huge therapeutic benefits. Nevertheless, data on the effects of the endemic species Origanum minutiflorum, also known as wild or Turkish oregano, is scarce. On the other hand, various chronic lung diseases, characterised by persistent inflammation, oxidative stress, and common bacterial infections, do not have effective pharmacological therapy. Hence, the aim of this study is to examine the effects of wild oregano essential oil (WOEO) on human bronchial epithelial cells and lung pathogens.MethodsWe provided a detailed chemical composition of WOEO using GC-MS and GC-FID analysis. Anti-inflammatory effects of WOEO were analysed using the qRT-PCR and ELISA methods, while antioxidative properties were examined by using the dichlorofluorescein assay in BEAS-2B cells. Antibacterial activity was tested on lung pathogens by using the agar diffusion assay.ResultsThe major constituents of WOEO, analysed in this study, were carvacrol, linalool, p-cymene, γ-terpinene, and (E)-caryophyllene. We found that treatment with WOEO attenuated LPS-induced IL8 gene expression and hydrogen peroxide-induced oxidative stress in BEAS-2B cells. Moreover, WOEO showed an inhibitory effect on pathogenic bacteria Acinetobacter baumannii and highly resistant Klebsiella pneumoniae commonly seen in healthcare-associated pneumonia.ConclusionOur work presents new insights into the anti-inflammatory, antioxidative, and antimicrobial properties of WOEO which may be used as a simple and local treatment in various chronic lung diseases.",
publisher = "Elsevier",
journal = "Journal of Herbal Medicine",
title = "Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens",
volume = "39",
pages = "100651",
doi = "10.1016/j.hermed.2023.100651"
}
Veljović, K., Tešević, V., Mitrović, H.,& Stanković, M.. (2023). Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens. in Journal of Herbal Medicine
Elsevier., 39, 100651.
https://doi.org/10.1016/j.hermed.2023.100651
Veljović K, Tešević V, Mitrović H, Stanković M. Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens. in Journal of Herbal Medicine. 2023;39:100651.
doi:10.1016/j.hermed.2023.100651 .
Veljović, Katarina, Tešević, Vele, Mitrović, Hristina, Stanković, Marija, "Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens" in Journal of Herbal Medicine, 39 (2023):100651,
https://doi.org/10.1016/j.hermed.2023.100651 . .
1
1

Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines

Gospodinova, Z.; Antov, G.; Novaković, M.; Tešević, Vele; Krasteva, N.; Pavlov, D.; Valcheva-Kuzmanova, S.

(Sciendo, 2023)

TY  - JOUR
AU  - Gospodinova, Z.
AU  - Antov, G.
AU  - Novaković, M.
AU  - Tešević, Vele
AU  - Krasteva, N.
AU  - Pavlov, D.
AU  - Valcheva-Kuzmanova, S.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6218
AB  - AbstractBackground and objectiveCotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26).Materials and methodsCell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fluorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line.ResultsThe obtained results showed that fustin reduced the proliferation of all studied cell lines. The highest cytostatic effect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was least affected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell
PB  - Sciendo
T2  - Acta Medica Bulgarica
T1  - Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines
VL  - 50
IS  - 1
SP  - 27
EP  - 33
DO  - 10.2478/amb-2023-0004
ER  - 
@article{
author = "Gospodinova, Z. and Antov, G. and Novaković, M. and Tešević, Vele and Krasteva, N. and Pavlov, D. and Valcheva-Kuzmanova, S.",
year = "2023",
abstract = "AbstractBackground and objectiveCotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26).Materials and methodsCell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fluorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line.ResultsThe obtained results showed that fustin reduced the proliferation of all studied cell lines. The highest cytostatic effect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was least affected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell",
publisher = "Sciendo",
journal = "Acta Medica Bulgarica",
title = "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines",
volume = "50",
number = "1",
pages = "27-33",
doi = "10.2478/amb-2023-0004"
}
Gospodinova, Z., Antov, G., Novaković, M., Tešević, V., Krasteva, N., Pavlov, D.,& Valcheva-Kuzmanova, S.. (2023). Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica
Sciendo., 50(1), 27-33.
https://doi.org/10.2478/amb-2023-0004
Gospodinova Z, Antov G, Novaković M, Tešević V, Krasteva N, Pavlov D, Valcheva-Kuzmanova S. Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica. 2023;50(1):27-33.
doi:10.2478/amb-2023-0004 .
Gospodinova, Z., Antov, G., Novaković, M., Tešević, Vele, Krasteva, N., Pavlov, D., Valcheva-Kuzmanova, S., "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Scop. Against Breast and Colon Cancer Cell Lines" in Acta Medica Bulgarica, 50, no. 1 (2023):27-33,
https://doi.org/10.2478/amb-2023-0004 . .

Nutritivna vrijednost i osnovne fitohemijske osobine blitve (Beta vulgaris ssp. cicla L. Voss) gajene u različitim uslovima navodnjavanja i prihranjivanja

Ivanović, Ljubica

(Универзитет у Београду, Хемијски факултет, 2023)

TY  - THES
AU  - Ivanović, Ljubica
PY  - 2023
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=9369
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:31816/bdef:Content/download
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/130986249
UR  - https://nardus.mpn.gov.rs/handle/123456789/21930
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6239
AB  - U okviru ove doktorske disertacije proučavan je nutritivni i fitonutritivni sadržaj blitve koja je gajena u uslovima različitih režima đubrenja i navodnjavanja, pri čemu su ispitivane i funkcionalne osobine blitve u cilju utvrđivanja njenog potencijala kao funkcionalne hrane.U početnoj fazi eksperimenta određene su nutritivne, antinutritivne i fitonutritivne komponente u blitvi, kao i uticaj primjenjenih agrotehničkih mjera na pomenute komponente. Korišćenjem standardnih metoda za proksimativnu analizu (AOAC metode) utvrđeno je da svježa blitva ima sledeći sastav: voda- 89%, ukupni šećeri- 4,9%, proteini- 2,5%, pepeo- 1,8% i lipidi-0,45%. Savremenim instrumentalnim tehnikama (spektrofotometrija, ICP OES, tečna hromatografija) su kvantifikovane druge nutritivne, antinutritivne i fitonutritivne komponente u blitvi. Nađeno je da su prosječne koncentracije vitamina C, ukupnog hlorofila, karotenoida u 100 g svježe blitve su redom: 26,9 mg, 47,13 mg, 9,85 mg. Blitva ima visok sadržaj makro- i mikroelemenata od kojih su najzastupljeniji sledeći: kalijum (372,49 mg), kalcijum (178,6 mg), mangan (8,35 mg) i gvožđe (1,46 mg) izraženo na 100 g svježe blitve. Analizom ukupnog polifenolnog i flavonoidnog sadržaja pokazano je da je blitva bogata ovim fitonutrijentima, nakon čega su identifikovane i kvantifikovane četiri flavonoidne komponente: viteksin-2"-O-ksilozid, viteksin-6″-O-malonil-2″-O-ksilozid, izoramnetin-3,7-O-diglukozid i viteksin-6″-O-acetil-2″-O-ksilozid. Strukture ovih jedinjenja su potvrđene 1D i 2D NMR spektroskopijom (1H NMR,13 C NMR, COSY, HSQC, HMBC). Osim toga, određen je sadržaj nitrata u blitvi, koji je bio u granicama dozvoljenih vrijednosti.Statističkom analizom uticaja različitih režima navodnjavanja i đubrenja nađeno je da je đubrenje uticalo na prinos, morfološke karakteristike, količinu proteina, sadržaj fosfora, sadržaj izoramnetin-3,7-O-diglukozida, sadržaj hlorofila i vitamina C. Povećanjem količine primjenjene prihrane povećali su se svi pomenuti prametri, osim sadržaj fosfora i sadržaj izoramnetin-3,7-O-diglukozida, koji su bili niži sa povećanjem nivoa đubrenja. Interakcija navodnjavanja i đubrenja je značajno uticala na sadržaj Na i Cu i sadržaj viteksin-2"-O-ksilozida. Navodnjavanje nije imalo značajnog efekta na testirane parametre, osim na sadržaj nitrata, gdje je povećanjem nivoa navodnjavanja sadržaj nitrata u blitvi opadao, dok je povećanjem đubrenja rastao njihov sadržaj.Poslednja faza eksperimenta je obuhvatila ispitivanje antioksidativne, antiproliferativne i antimikrobne aktivnosti ekstrakta blitve. Rezultati su pokazali antioksidativnu aktivnost vodenog ekstrakta blitve u vrijednosti IC50=3,79 mg/ml (mjereno DPPH testom) dok je metanolni ekstrakt imao prosječni antioksidativni kapacitet od 8,0 (mjereno DPPH testom) odnosno 8,57 μmola ekvivalenta trolox-a/g (mjereno ABTS testom). Analizom antiproliferativne aktivnosti ekstrakta blitve je utvrđeno da postoji inhibicija rasta malignih MCF-7 ćelija i to 70% ćelija je inhibirano nakon 48 h inkubacije. Ispitivani ekstrakti blitve nijesu pokazali antimikrobnu aktivnost.
AB  - In this doctoral dissertation, the nutritional and phytonutrient content of Swiss chard grown under different irrigation and fertilization regimes was studied, while the functional properties of Swiss chard were also examined in order to determine its potential as a functional food.In the initial phase of the experiment, nutritional, anti-nutritive and phyto-nutritive components in Swiss chard were determined, as well as the influence of applied agrotechnical measurments on all tested parameters was monitored. Using standard methods for proximate analysis (AOAC methods), it was determined that fresh chard has the following composition: water- 89%, total sugars- 4,9 %, proteins- 2,5 %, ash- 1,8 % and lipids- 0,45%. Using modern instrumental techniques (spectrophotometry, ICP OES, liquid chromatography) other nutritional and phytonutritive components in Swiss chard were quantified. It was found that the average concentrations of vitamin C, total chlorophyll, carotenoids in 100 g of fresh chard are: 26,9 mg, 47,13 mg and 9,85 mg, respectively. Swiss chard has a high content of macro- and microelements, of which the most abundant are the following: potassium (372,49 mg), calcium (178,6 mg), followed by manganese (8,35 mg) and iron (1,46 mg) expressed per 100 g of fresh Swiss chard. The analysis of the total polyphenolic and flavonoid content showed that Swiss chard is rich in these phytonutrients, after which four flavonoid components were identified and quantified: vitexin-2"-O-xyloside, vitexin-6"-O-malonyl-2"-O-xyloside, isorhamnetin-3,7-O-diglucoside and vitexin-6″-O-acetyl-2″-O-xyloside. The structures of these compounds were confirmed by 1D and 2D NMR spectroscopy (1H NMR,13 C NMR, COSY, HSQC, HMBC). In addition, the content of nitrates in Swiss chard was determined, which was within the limits of permitted values.A statistical analysis of the effect of different irrigation and fertilization regimes found that fertilization influenced the yield, morphological characteristics, amount of protein, as well as phosphorus, isorhamnetin-3,7-O-diglucoside, chlorophyll, and vitamin C content. With increasing the amount of fertilization, values for all parameters mentioned above increased, except phosphorus and isorhamnetin-3,7-O-diglucoside content, which were found to be lower with increasing fertilization level. The interaction of irrigation and fertilization significantly affected Na and Cu content, as well as the content of vitexin-2"-O-xyloside. Irrigation had no significant effect on the studied parameters, except for the nitrate content. While the increased level of irrigation led to decreased nitrate content in Swiss chard, with increasing fertilization their content increased.The last phase of the experiment included investigation of the antioxidant, antiproliferative and antimicrobial activity of Swiss chard extract. The results showed the antioxidant activity of the aqueous extract of Swiss chard with an IC50 value of 3,79 mg/ml (measured by the DPPH test), while the methanolic extract had an average antioxidant capacity of 8,0 (measured by the DPPH test), 8,57 μmol of trolox equivalents per gram (measured by ABTS test). Analysis of the antiproliferative activity of Swiss chard extract revealed that there is an inhibition of the growth of malignant MCF-7 cells and that 70% of the cells were inhibited after 48 hours of incubation. The studied Swiss chard extracts did not show antimicrobial activity.
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Nutritivna vrijednost i osnovne fitohemijske osobine blitve (Beta vulgaris ssp. cicla L. Voss) gajene u različitim uslovima navodnjavanja i prihranjivanja
UR  - https://hdl.handle.net/21.15107/rcub_nardus_21930
ER  - 
@phdthesis{
author = "Ivanović, Ljubica",
year = "2023",
abstract = "U okviru ove doktorske disertacije proučavan je nutritivni i fitonutritivni sadržaj blitve koja je gajena u uslovima različitih režima đubrenja i navodnjavanja, pri čemu su ispitivane i funkcionalne osobine blitve u cilju utvrđivanja njenog potencijala kao funkcionalne hrane.U početnoj fazi eksperimenta određene su nutritivne, antinutritivne i fitonutritivne komponente u blitvi, kao i uticaj primjenjenih agrotehničkih mjera na pomenute komponente. Korišćenjem standardnih metoda za proksimativnu analizu (AOAC metode) utvrđeno je da svježa blitva ima sledeći sastav: voda- 89%, ukupni šećeri- 4,9%, proteini- 2,5%, pepeo- 1,8% i lipidi-0,45%. Savremenim instrumentalnim tehnikama (spektrofotometrija, ICP OES, tečna hromatografija) su kvantifikovane druge nutritivne, antinutritivne i fitonutritivne komponente u blitvi. Nađeno je da su prosječne koncentracije vitamina C, ukupnog hlorofila, karotenoida u 100 g svježe blitve su redom: 26,9 mg, 47,13 mg, 9,85 mg. Blitva ima visok sadržaj makro- i mikroelemenata od kojih su najzastupljeniji sledeći: kalijum (372,49 mg), kalcijum (178,6 mg), mangan (8,35 mg) i gvožđe (1,46 mg) izraženo na 100 g svježe blitve. Analizom ukupnog polifenolnog i flavonoidnog sadržaja pokazano je da je blitva bogata ovim fitonutrijentima, nakon čega su identifikovane i kvantifikovane četiri flavonoidne komponente: viteksin-2"-O-ksilozid, viteksin-6″-O-malonil-2″-O-ksilozid, izoramnetin-3,7-O-diglukozid i viteksin-6″-O-acetil-2″-O-ksilozid. Strukture ovih jedinjenja su potvrđene 1D i 2D NMR spektroskopijom (1H NMR,13 C NMR, COSY, HSQC, HMBC). Osim toga, određen je sadržaj nitrata u blitvi, koji je bio u granicama dozvoljenih vrijednosti.Statističkom analizom uticaja različitih režima navodnjavanja i đubrenja nađeno je da je đubrenje uticalo na prinos, morfološke karakteristike, količinu proteina, sadržaj fosfora, sadržaj izoramnetin-3,7-O-diglukozida, sadržaj hlorofila i vitamina C. Povećanjem količine primjenjene prihrane povećali su se svi pomenuti prametri, osim sadržaj fosfora i sadržaj izoramnetin-3,7-O-diglukozida, koji su bili niži sa povećanjem nivoa đubrenja. Interakcija navodnjavanja i đubrenja je značajno uticala na sadržaj Na i Cu i sadržaj viteksin-2"-O-ksilozida. Navodnjavanje nije imalo značajnog efekta na testirane parametre, osim na sadržaj nitrata, gdje je povećanjem nivoa navodnjavanja sadržaj nitrata u blitvi opadao, dok je povećanjem đubrenja rastao njihov sadržaj.Poslednja faza eksperimenta je obuhvatila ispitivanje antioksidativne, antiproliferativne i antimikrobne aktivnosti ekstrakta blitve. Rezultati su pokazali antioksidativnu aktivnost vodenog ekstrakta blitve u vrijednosti IC50=3,79 mg/ml (mjereno DPPH testom) dok je metanolni ekstrakt imao prosječni antioksidativni kapacitet od 8,0 (mjereno DPPH testom) odnosno 8,57 μmola ekvivalenta trolox-a/g (mjereno ABTS testom). Analizom antiproliferativne aktivnosti ekstrakta blitve je utvrđeno da postoji inhibicija rasta malignih MCF-7 ćelija i to 70% ćelija je inhibirano nakon 48 h inkubacije. Ispitivani ekstrakti blitve nijesu pokazali antimikrobnu aktivnost., In this doctoral dissertation, the nutritional and phytonutrient content of Swiss chard grown under different irrigation and fertilization regimes was studied, while the functional properties of Swiss chard were also examined in order to determine its potential as a functional food.In the initial phase of the experiment, nutritional, anti-nutritive and phyto-nutritive components in Swiss chard were determined, as well as the influence of applied agrotechnical measurments on all tested parameters was monitored. Using standard methods for proximate analysis (AOAC methods), it was determined that fresh chard has the following composition: water- 89%, total sugars- 4,9 %, proteins- 2,5 %, ash- 1,8 % and lipids- 0,45%. Using modern instrumental techniques (spectrophotometry, ICP OES, liquid chromatography) other nutritional and phytonutritive components in Swiss chard were quantified. It was found that the average concentrations of vitamin C, total chlorophyll, carotenoids in 100 g of fresh chard are: 26,9 mg, 47,13 mg and 9,85 mg, respectively. Swiss chard has a high content of macro- and microelements, of which the most abundant are the following: potassium (372,49 mg), calcium (178,6 mg), followed by manganese (8,35 mg) and iron (1,46 mg) expressed per 100 g of fresh Swiss chard. The analysis of the total polyphenolic and flavonoid content showed that Swiss chard is rich in these phytonutrients, after which four flavonoid components were identified and quantified: vitexin-2"-O-xyloside, vitexin-6"-O-malonyl-2"-O-xyloside, isorhamnetin-3,7-O-diglucoside and vitexin-6″-O-acetyl-2″-O-xyloside. The structures of these compounds were confirmed by 1D and 2D NMR spectroscopy (1H NMR,13 C NMR, COSY, HSQC, HMBC). In addition, the content of nitrates in Swiss chard was determined, which was within the limits of permitted values.A statistical analysis of the effect of different irrigation and fertilization regimes found that fertilization influenced the yield, morphological characteristics, amount of protein, as well as phosphorus, isorhamnetin-3,7-O-diglucoside, chlorophyll, and vitamin C content. With increasing the amount of fertilization, values for all parameters mentioned above increased, except phosphorus and isorhamnetin-3,7-O-diglucoside content, which were found to be lower with increasing fertilization level. The interaction of irrigation and fertilization significantly affected Na and Cu content, as well as the content of vitexin-2"-O-xyloside. Irrigation had no significant effect on the studied parameters, except for the nitrate content. While the increased level of irrigation led to decreased nitrate content in Swiss chard, with increasing fertilization their content increased.The last phase of the experiment included investigation of the antioxidant, antiproliferative and antimicrobial activity of Swiss chard extract. The results showed the antioxidant activity of the aqueous extract of Swiss chard with an IC50 value of 3,79 mg/ml (measured by the DPPH test), while the methanolic extract had an average antioxidant capacity of 8,0 (measured by the DPPH test), 8,57 μmol of trolox equivalents per gram (measured by ABTS test). Analysis of the antiproliferative activity of Swiss chard extract revealed that there is an inhibition of the growth of malignant MCF-7 cells and that 70% of the cells were inhibited after 48 hours of incubation. The studied Swiss chard extracts did not show antimicrobial activity.",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Nutritivna vrijednost i osnovne fitohemijske osobine blitve (Beta vulgaris ssp. cicla L. Voss) gajene u različitim uslovima navodnjavanja i prihranjivanja",
url = "https://hdl.handle.net/21.15107/rcub_nardus_21930"
}
Ivanović, L.. (2023). Nutritivna vrijednost i osnovne fitohemijske osobine blitve (Beta vulgaris ssp. cicla L. Voss) gajene u različitim uslovima navodnjavanja i prihranjivanja. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_21930
Ivanović L. Nutritivna vrijednost i osnovne fitohemijske osobine blitve (Beta vulgaris ssp. cicla L. Voss) gajene u različitim uslovima navodnjavanja i prihranjivanja. in Универзитет у Београду. 2023;.
https://hdl.handle.net/21.15107/rcub_nardus_21930 .
Ivanović, Ljubica, "Nutritivna vrijednost i osnovne fitohemijske osobine blitve (Beta vulgaris ssp. cicla L. Voss) gajene u različitim uslovima navodnjavanja i prihranjivanja" in Универзитет у Београду (2023),
https://hdl.handle.net/21.15107/rcub_nardus_21930 .