All Publications

Link to this page

Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481

Šegan, Sandra B.; Jevtić, Ivana; Tosti, Tomislav; Penjišević, Jelena; Šukalović, Vladimir; Kostić-Rajačić, Slađana; Milojković-Opsenica, Dušanka

TY  - DATA
AU  - Šegan, Sandra B.
AU  - Jevtić, Ivana
AU  - Tosti, Tomislav
AU  - Penjišević, Jelena
AU  - Šukalović, Vladimir
AU  - Kostić-Rajačić, Slađana
AU  - Milojković-Opsenica, Dušanka
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5650
AB  - Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of thepiperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constantof fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-ophilicity, expressed as retention indices RM0 , b, and C0, as well as PC1, was determined and correlated with insilico values. Ionization constants were determined on the basis of the relationships between analyte’s retentionexpressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, weresubjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to providebasic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeationwas investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-lecular weight. In general, results of the present research corroborate well with previously determined anti-nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, anotherset of important parameters should be taken into account when designing new derivatives of C-3 substitutedfentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLCcan be considered as a valuable asset in the ligand-based drug design
PB  - Elsevier
T2  - Journal of Chromatography B
T1  - Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481
VL  - 1211
SP  - 123481
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5650
ER  - 
@misc{
author = "Šegan, Sandra B. and Jevtić, Ivana and Tosti, Tomislav and Penjišević, Jelena and Šukalović, Vladimir and Kostić-Rajačić, Slađana and Milojković-Opsenica, Dušanka",
abstract = "Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of thepiperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constantof fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-ophilicity, expressed as retention indices RM0 , b, and C0, as well as PC1, was determined and correlated with insilico values. Ionization constants were determined on the basis of the relationships between analyte’s retentionexpressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, weresubjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to providebasic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeationwas investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-lecular weight. In general, results of the present research corroborate well with previously determined anti-nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, anotherset of important parameters should be taken into account when designing new derivatives of C-3 substitutedfentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLCcan be considered as a valuable asset in the ligand-based drug design",
publisher = "Elsevier",
journal = "Journal of Chromatography B",
title = "Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481",
volume = "1211",
pages = "123481",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5650"
}
Šegan, S. B., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D..Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481. in Journal of Chromatography B
Elsevier., 1211, 123481.
https://hdl.handle.net/21.15107/rcub_cherry_5650
Šegan SB, Jevtić I, Tosti T, Penjišević J, Šukalović V, Kostić-Rajačić S, Milojković-Opsenica D. Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481. in Journal of Chromatography B.1211:123481.
https://hdl.handle.net/21.15107/rcub_cherry_5650 .
Šegan, Sandra B., Jevtić, Ivana, Tosti, Tomislav, Penjišević, Jelena, Šukalović, Vladimir, Kostić-Rajačić, Slađana, Milojković-Opsenica, Dušanka, "Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481" in Journal of Chromatography B, 1211:123481,
https://hdl.handle.net/21.15107/rcub_cherry_5650 .

Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184

Krstić, Đurđa D.; Ristivojević, Petar; Gašić, Uroš M.; Lazović, Mila; Fotirić Akšić, Milica M.; Milivojević, Jasminka; Morlock, Gertrud E.; Milojković-Opsenica, Dušanka; Trifković, Jelena

(Elsevier, 2023)

TY  - DATA
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5633
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184
VL  - 402
SP  - 134184
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5633
ER  - 
@misc{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184",
volume = "402",
pages = "134184",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5633"
}
Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D.,& Trifković, J.. (2023). Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry
Elsevier., 402, 134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633
Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica D, Trifković J. Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry. 2023;402:134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633 .
Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka, Trifković, Jelena, "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184" in Food Chemistry, 402 (2023):134184,
https://hdl.handle.net/21.15107/rcub_cherry_5633 .

Authenticity assessment of cultivated berries via phenolic profiles of seeds

Krstić, Đurđa D.; Ristivojević, Petar; Gašić, Uroš M.; Lazović, Mila; Fotirić Akšić, Milica M.; Milivojević, Jasminka; Morlock, Gertrud E.; Milojković-Opsenica, Dušanka; Trifković, Jelena

(Elsevier, 2023)

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/5633
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Authenticity assessment of cultivated berries via phenolic profiles of seeds
VL  - 402
SP  - 134184
DO  - 10.1016/j.foodchem.2022.134184
ER  - 
@article{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Authenticity assessment of cultivated berries via phenolic profiles of seeds",
volume = "402",
pages = "134184",
doi = "10.1016/j.foodchem.2022.134184"
}
Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D.,& Trifković, J.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry
Elsevier., 402, 134184.
https://doi.org/10.1016/j.foodchem.2022.134184
Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica D, Trifković J. Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry. 2023;402:134184.
doi:10.1016/j.foodchem.2022.134184 .
Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka, Trifković, Jelena, "Authenticity assessment of cultivated berries via phenolic profiles of seeds" in Food Chemistry, 402 (2023):134184,
https://doi.org/10.1016/j.foodchem.2022.134184 . .

Gamma rays induced synthesis of graphene oxide/gold nanoparticle composites: structural and photothermal study

Kepić, Dejan P.; Stefanović, Anđela M.; Budimir, Milica D.; Pavlović, Vladimir B.; Bonasera, Aurelio; Scopelliti, Michelangelo; Todorović-Marković, Biljana

(Elsevier, 2023)

TY  - JOUR
AU  - Kepić, Dejan P.
AU  - Stefanović, Anđela M.
AU  - Budimir, Milica D.
AU  - Pavlović, Vladimir B.
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Todorović-Marković, Biljana
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5627
AB  - Gamma irradiation provides an alternative pathway to conventional gold nanoparticle synthesis because it is simple, fast, and economical. Here, we employed gamma irradiation at low doses (1–20 kGy) to obtain gold nanoparticles (Au NPs) anchored onto graphene oxide (GO) sheets. GO was selected as a suitable platform for the nucleation and growth of Au NPs because of its large surface area and good dispersibility in water due to the presence of polar oxygen-containing functional groups in its structure. Gamma irradiation at all the applied doses led to the reduction of chloroauric acid and the formation of evenly distributed Au NPs at the GO surface, simultaneously causing the reduction of GO and partial restoration of the graphene structure. As-prepared Au NPs have predominately spheric shapes and the smallest nanoparticles were reported for the dose of 1 kGy. The increase in the irradiation dose caused either the growth of larger particles (5 and 10 kGy) or the broad distribution of particles’ sizes (20 kGy). All samples showed a temperature increase upon exposure to 800 nm laser and photothermal efficiency was the highest for the sample prepared at 20 kGy.
PB  - Elsevier
T2  - Radiation Physics and Chemistry
T1  - Gamma rays induced synthesis of graphene oxide/gold nanoparticle composites: structural and photothermal study
VL  - 202
SP  - 110545
DO  - 10.1016/j.radphyschem.2022.110545
ER  - 
@article{
author = "Kepić, Dejan P. and Stefanović, Anđela M. and Budimir, Milica D. and Pavlović, Vladimir B. and Bonasera, Aurelio and Scopelliti, Michelangelo and Todorović-Marković, Biljana",
year = "2023",
abstract = "Gamma irradiation provides an alternative pathway to conventional gold nanoparticle synthesis because it is simple, fast, and economical. Here, we employed gamma irradiation at low doses (1–20 kGy) to obtain gold nanoparticles (Au NPs) anchored onto graphene oxide (GO) sheets. GO was selected as a suitable platform for the nucleation and growth of Au NPs because of its large surface area and good dispersibility in water due to the presence of polar oxygen-containing functional groups in its structure. Gamma irradiation at all the applied doses led to the reduction of chloroauric acid and the formation of evenly distributed Au NPs at the GO surface, simultaneously causing the reduction of GO and partial restoration of the graphene structure. As-prepared Au NPs have predominately spheric shapes and the smallest nanoparticles were reported for the dose of 1 kGy. The increase in the irradiation dose caused either the growth of larger particles (5 and 10 kGy) or the broad distribution of particles’ sizes (20 kGy). All samples showed a temperature increase upon exposure to 800 nm laser and photothermal efficiency was the highest for the sample prepared at 20 kGy.",
publisher = "Elsevier",
journal = "Radiation Physics and Chemistry",
title = "Gamma rays induced synthesis of graphene oxide/gold nanoparticle composites: structural and photothermal study",
volume = "202",
pages = "110545",
doi = "10.1016/j.radphyschem.2022.110545"
}
Kepić, D. P., Stefanović, A. M., Budimir, M. D., Pavlović, V. B., Bonasera, A., Scopelliti, M.,& Todorović-Marković, B.. (2023). Gamma rays induced synthesis of graphene oxide/gold nanoparticle composites: structural and photothermal study. in Radiation Physics and Chemistry
Elsevier., 202, 110545.
https://doi.org/10.1016/j.radphyschem.2022.110545
Kepić DP, Stefanović AM, Budimir MD, Pavlović VB, Bonasera A, Scopelliti M, Todorović-Marković B. Gamma rays induced synthesis of graphene oxide/gold nanoparticle composites: structural and photothermal study. in Radiation Physics and Chemistry. 2023;202:110545.
doi:10.1016/j.radphyschem.2022.110545 .
Kepić, Dejan P., Stefanović, Anđela M., Budimir, Milica D., Pavlović, Vladimir B., Bonasera, Aurelio, Scopelliti, Michelangelo, Todorović-Marković, Biljana, "Gamma rays induced synthesis of graphene oxide/gold nanoparticle composites: structural and photothermal study" in Radiation Physics and Chemistry, 202 (2023):110545,
https://doi.org/10.1016/j.radphyschem.2022.110545 . .

Supplementary material for: Aćimović, M., Šovljanski, O., Pezo, L., Travičić, V., Tomić, A., Zheljazkov, V. D., Ćetković, G., Švarc-Gajić, J., Brezo-Borjan, T.,& Sofrenić, I.. (2022). Variability in Biological Activities of Satureja montana Subsp. montana and Subsp. variegata Based on Different Extraction Methods. in Antibiotics MDPI., 11, 1235. https://doi.org/10.3390/antibiotics11091235

Aćimović, Milica; Šovljanski, Olja; Pezo, Lato; Travičić, Vanja; Tomić, Ana; Zheljazkov, Valtcho D.; Ćetković, Gordana; Švarc-Gajić, Jaroslava; Brezo-Borjan, Tanja; Sofrenić, Ivana

(MDPI, 2022)

TY  - DATA
AU  - Aćimović, Milica
AU  - Šovljanski, Olja
AU  - Pezo, Lato
AU  - Travičić, Vanja
AU  - Tomić, Ana
AU  - Zheljazkov, Valtcho D.
AU  - Ćetković, Gordana
AU  - Švarc-Gajić, Jaroslava
AU  - Brezo-Borjan, Tanja
AU  - Sofrenić, Ivana
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5661
AB  - Winter savory (Satureja montana L.) is a well-known spice and medicinal plant with a wide range of activities and applications. Two subspecies of S. montana, subsp. montana and subsp. variegata, were used for the preparation of seven different extracts: steam distillation (essential oil (EO) and hydrolate (HY)), subcritical water (SWE), ultrasound-assisted (UAE-MeOH and UAE-H2O), and microwave-assisted (MAE-MeOH and MAE-H2O) extraction. The obtained EOs, HYs, and extracts were used for an in vitro evaluation of the antioxidant activity (DPPH, ABTS, reducing power, and superoxide anion methods) and in vitro antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella Typhimurium, Saccharomyces cerevisiae, and Candida albicans. The antimicrobial screening was conducted using disk-diffusion assessment, minimal inhibitory concentration, time–kill kinetics modeling, and pharmacodynamic study of the biocide effect. The total phenolic content (TPC) was highest in EO, followed by SWE, MAE, and UAE, and the lowest was in HY. The highest antimicrobial activity shows EO and SWE for both varieties, while different UAE and MAE extracts have not exhibited antimicrobial activity. The natural antimicrobials in the S. montana extract samples obtained by green extraction methods, indicated the possibility of ecologically and economically better solutions for future in vivo application of the selected plant subspecies.
PB  - MDPI
T2  - Antibiotics
T1  - Supplementary material for: Aćimović, M., Šovljanski, O., Pezo, L., Travičić, V., Tomić, A., Zheljazkov, V. D., Ćetković, G., Švarc-Gajić, J., Brezo-Borjan, T.,& Sofrenić, I.. (2022). Variability in Biological Activities of Satureja montana Subsp. montana and Subsp. variegata Based on Different Extraction Methods. in Antibiotics MDPI., 11, 1235. https://doi.org/10.3390/antibiotics11091235
VL  - 11
SP  - 1235
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5661
ER  - 
@misc{
author = "Aćimović, Milica and Šovljanski, Olja and Pezo, Lato and Travičić, Vanja and Tomić, Ana and Zheljazkov, Valtcho D. and Ćetković, Gordana and Švarc-Gajić, Jaroslava and Brezo-Borjan, Tanja and Sofrenić, Ivana",
year = "2022",
abstract = "Winter savory (Satureja montana L.) is a well-known spice and medicinal plant with a wide range of activities and applications. Two subspecies of S. montana, subsp. montana and subsp. variegata, were used for the preparation of seven different extracts: steam distillation (essential oil (EO) and hydrolate (HY)), subcritical water (SWE), ultrasound-assisted (UAE-MeOH and UAE-H2O), and microwave-assisted (MAE-MeOH and MAE-H2O) extraction. The obtained EOs, HYs, and extracts were used for an in vitro evaluation of the antioxidant activity (DPPH, ABTS, reducing power, and superoxide anion methods) and in vitro antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella Typhimurium, Saccharomyces cerevisiae, and Candida albicans. The antimicrobial screening was conducted using disk-diffusion assessment, minimal inhibitory concentration, time–kill kinetics modeling, and pharmacodynamic study of the biocide effect. The total phenolic content (TPC) was highest in EO, followed by SWE, MAE, and UAE, and the lowest was in HY. The highest antimicrobial activity shows EO and SWE for both varieties, while different UAE and MAE extracts have not exhibited antimicrobial activity. The natural antimicrobials in the S. montana extract samples obtained by green extraction methods, indicated the possibility of ecologically and economically better solutions for future in vivo application of the selected plant subspecies.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Supplementary material for: Aćimović, M., Šovljanski, O., Pezo, L., Travičić, V., Tomić, A., Zheljazkov, V. D., Ćetković, G., Švarc-Gajić, J., Brezo-Borjan, T.,& Sofrenić, I.. (2022). Variability in Biological Activities of Satureja montana Subsp. montana and Subsp. variegata Based on Different Extraction Methods. in Antibiotics MDPI., 11, 1235. https://doi.org/10.3390/antibiotics11091235",
volume = "11",
pages = "1235",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5661"
}
Aćimović, M., Šovljanski, O., Pezo, L., Travičić, V., Tomić, A., Zheljazkov, V. D., Ćetković, G., Švarc-Gajić, J., Brezo-Borjan, T.,& Sofrenić, I.. (2022). Supplementary material for: Aćimović, M., Šovljanski, O., Pezo, L., Travičić, V., Tomić, A., Zheljazkov, V. D., Ćetković, G., Švarc-Gajić, J., Brezo-Borjan, T.,& Sofrenić, I.. (2022). Variability in Biological Activities of Satureja montana Subsp. montana and Subsp. variegata Based on Different Extraction Methods. in Antibiotics MDPI., 11, 1235. https://doi.org/10.3390/antibiotics11091235. in Antibiotics
MDPI., 11, 1235.
https://hdl.handle.net/21.15107/rcub_cherry_5661
Aćimović M, Šovljanski O, Pezo L, Travičić V, Tomić A, Zheljazkov VD, Ćetković G, Švarc-Gajić J, Brezo-Borjan T, Sofrenić I. Supplementary material for: Aćimović, M., Šovljanski, O., Pezo, L., Travičić, V., Tomić, A., Zheljazkov, V. D., Ćetković, G., Švarc-Gajić, J., Brezo-Borjan, T.,& Sofrenić, I.. (2022). Variability in Biological Activities of Satureja montana Subsp. montana and Subsp. variegata Based on Different Extraction Methods. in Antibiotics MDPI., 11, 1235. https://doi.org/10.3390/antibiotics11091235. in Antibiotics. 2022;11:1235.
https://hdl.handle.net/21.15107/rcub_cherry_5661 .
Aćimović, Milica, Šovljanski, Olja, Pezo, Lato, Travičić, Vanja, Tomić, Ana, Zheljazkov, Valtcho D., Ćetković, Gordana, Švarc-Gajić, Jaroslava, Brezo-Borjan, Tanja, Sofrenić, Ivana, "Supplementary material for: Aćimović, M., Šovljanski, O., Pezo, L., Travičić, V., Tomić, A., Zheljazkov, V. D., Ćetković, G., Švarc-Gajić, J., Brezo-Borjan, T.,& Sofrenić, I.. (2022). Variability in Biological Activities of Satureja montana Subsp. montana and Subsp. variegata Based on Different Extraction Methods. in Antibiotics MDPI., 11, 1235. https://doi.org/10.3390/antibiotics11091235" in Antibiotics, 11 (2022):1235,
https://hdl.handle.net/21.15107/rcub_cherry_5661 .

Supplementary material for: Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2022). Efficiency of Natural Deep Eutectic Solvents to Extract Phenolic Compounds from Agrimonia eupatoria: Experimental Study and In Silico Modelling. in Plants MDPI., 11, 2346. https://doi.org/10.3390/plants11182346

Lazović, Mila; Cvijetić, Ilija; Jankov, Milica; Milojković-Opsenica, Dušanka; Trifković, Jelena; Ristivojević, Petar

(MDPI, 2022)

TY  - DATA
AU  - Lazović, Mila
AU  - Cvijetić, Ilija
AU  - Jankov, Milica
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
AU  - Ristivojević, Petar
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5659
AB  - To replace common organic solvents that present inherent toxicity and have high volatility and to improve the extraction efficiency, a range of natural deep eutectic solvents (NADESs) were evaluated for the extraction of phenolic compounds from Agrimonia eupatoria. Screening of NADES efficiency was carried out based on the total phenolic and flavonoid content and radical-scavenging activity, determined by spectrophotometry, as well as phenolic compounds quantified, obtained using ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer. Increased extraction efficiency when compared with organic solvent was achieved using NADES mixtures choline chloride (ChCl):urea 1:2 and choline chloride:glycerol 1:1. Flavonol glycosides were the most abundant compounds in all extracts. The COSMO-RS model provided insights into the most important intermolecular interactions that drive the extraction process. Moreover, it could explain the extraction efficiency of flavonol glycosides using ChCl:glycerol NADES. The current article offers experimental evidence and mechanistic insights for the selection of optimal NADES to extract bioactive components from Agrimonia eupatoria. © 2022 by the authors.
PB  - MDPI
T2  - Plants
T1  - Supplementary material for: Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2022). Efficiency of Natural Deep Eutectic Solvents to Extract Phenolic Compounds from Agrimonia eupatoria: Experimental Study and In Silico Modelling. in Plants MDPI., 11, 2346. https://doi.org/10.3390/plants11182346
VL  - 11
SP  - 2346
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5659
ER  - 
@misc{
author = "Lazović, Mila and Cvijetić, Ilija and Jankov, Milica and Milojković-Opsenica, Dušanka and Trifković, Jelena and Ristivojević, Petar",
year = "2022",
abstract = "To replace common organic solvents that present inherent toxicity and have high volatility and to improve the extraction efficiency, a range of natural deep eutectic solvents (NADESs) were evaluated for the extraction of phenolic compounds from Agrimonia eupatoria. Screening of NADES efficiency was carried out based on the total phenolic and flavonoid content and radical-scavenging activity, determined by spectrophotometry, as well as phenolic compounds quantified, obtained using ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer. Increased extraction efficiency when compared with organic solvent was achieved using NADES mixtures choline chloride (ChCl):urea 1:2 and choline chloride:glycerol 1:1. Flavonol glycosides were the most abundant compounds in all extracts. The COSMO-RS model provided insights into the most important intermolecular interactions that drive the extraction process. Moreover, it could explain the extraction efficiency of flavonol glycosides using ChCl:glycerol NADES. The current article offers experimental evidence and mechanistic insights for the selection of optimal NADES to extract bioactive components from Agrimonia eupatoria. © 2022 by the authors.",
publisher = "MDPI",
journal = "Plants",
title = "Supplementary material for: Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2022). Efficiency of Natural Deep Eutectic Solvents to Extract Phenolic Compounds from Agrimonia eupatoria: Experimental Study and In Silico Modelling. in Plants MDPI., 11, 2346. https://doi.org/10.3390/plants11182346",
volume = "11",
pages = "2346",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5659"
}
Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2022). Supplementary material for: Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2022). Efficiency of Natural Deep Eutectic Solvents to Extract Phenolic Compounds from Agrimonia eupatoria: Experimental Study and In Silico Modelling. in Plants MDPI., 11, 2346. https://doi.org/10.3390/plants11182346. in Plants
MDPI., 11, 2346.
https://hdl.handle.net/21.15107/rcub_cherry_5659
Lazović M, Cvijetić I, Jankov M, Milojković-Opsenica D, Trifković J, Ristivojević P. Supplementary material for: Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2022). Efficiency of Natural Deep Eutectic Solvents to Extract Phenolic Compounds from Agrimonia eupatoria: Experimental Study and In Silico Modelling. in Plants MDPI., 11, 2346. https://doi.org/10.3390/plants11182346. in Plants. 2022;11:2346.
https://hdl.handle.net/21.15107/rcub_cherry_5659 .
Lazović, Mila, Cvijetić, Ilija, Jankov, Milica, Milojković-Opsenica, Dušanka, Trifković, Jelena, Ristivojević, Petar, "Supplementary material for: Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2022). Efficiency of Natural Deep Eutectic Solvents to Extract Phenolic Compounds from Agrimonia eupatoria: Experimental Study and In Silico Modelling. in Plants MDPI., 11, 2346. https://doi.org/10.3390/plants11182346" in Plants, 11 (2022):2346,
https://hdl.handle.net/21.15107/rcub_cherry_5659 .

Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profling Microbial Glycans

Dragačević, Luka; Lopandić, Zorana; Gavrović-Jankulović, Marija; Živković, Irena; Blagojević, Veljko; Polović, Natalija; Minić, Rajna

(Springer, 2022)

TY  - JOUR
AU  - Dragačević, Luka
AU  - Lopandić, Zorana
AU  - Gavrović-Jankulović, Marija
AU  - Živković, Irena
AU  - Blagojević, Veljko
AU  - Polović, Natalija
AU  - Minić, Rajna
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4887
AB  - The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T2  - Applied Biochemistry and BiotechnologyAppl Biochem Biotechnol
T1  - Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans
VL  - n/a
DO  - 10.1007/s12010-021-03772-w
ER  - 
@article{
author = "Dragačević, Luka and Lopandić, Zorana and Gavrović-Jankulović, Marija and Živković, Irena and Blagojević, Veljko and Polović, Natalija and Minić, Rajna",
year = "2022",
abstract = "The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology, Applied Biochemistry and BiotechnologyAppl Biochem Biotechnol",
title = "Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans",
volume = "n/a",
doi = "10.1007/s12010-021-03772-w"
}
Dragačević, L., Lopandić, Z., Gavrović-Jankulović, M., Živković, I., Blagojević, V., Polović, N.,& Minić, R.. (2022). Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans. in Applied Biochemistry and Biotechnology
Springer., n/a.
https://doi.org/10.1007/s12010-021-03772-w
Dragačević L, Lopandić Z, Gavrović-Jankulović M, Živković I, Blagojević V, Polović N, Minić R. Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans. in Applied Biochemistry and Biotechnology. 2022;n/a.
doi:10.1007/s12010-021-03772-w .
Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, "Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans" in Applied Biochemistry and Biotechnology, n/a (2022),
https://doi.org/10.1007/s12010-021-03772-w . .
1
1

Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.

Dragačević, Luka; Lopandić, Zorana; Gavrović-Jankulović, Marija; Živković, Irena; Blagojević, Veljko; Polović, Natalija; Minić, Rajna

(Springer, 2022)

TY  - DATA
AU  - Dragačević, Luka
AU  - Lopandić, Zorana
AU  - Gavrović-Jankulović, Marija
AU  - Živković, Irena
AU  - Blagojević, Veljko
AU  - Polović, Natalija
AU  - Minić, Rajna
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4888
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T1  - Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4888
ER  - 
@misc{
author = "Dragačević, Luka and Lopandić, Zorana and Gavrović-Jankulović, Marija and Živković, Irena and Blagojević, Veljko and Polović, Natalija and Minić, Rajna",
year = "2022",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology",
title = "Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4888"
}
Dragačević, L., Lopandić, Z., Gavrović-Jankulović, M., Živković, I., Blagojević, V., Polović, N.,& Minić, R.. (2022). Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.. in Applied Biochemistry and Biotechnology
Springer..
https://hdl.handle.net/21.15107/rcub_cherry_4888
Dragačević L, Lopandić Z, Gavrović-Jankulović M, Živković I, Blagojević V, Polović N, Minić R. Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.. in Applied Biochemistry and Biotechnology. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_4888 .
Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, "Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w." in Applied Biochemistry and Biotechnology (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4888 .

Izrada alata za izračunavanje dostupne površine proteina u rastvaraču (SASA)

Đarmati, Deneš

(2022)

TY  - THES
AU  - Đarmati, Deneš
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5013
AB  - U ovom radu će biti opisani algoritmi za analizu geometrijske strukture proteina, njihova
efikasnost i metode optimizacije.
T1  - Izrada alata za izračunavanje dostupne površine proteina u rastvaraču (SASA)
SP  - 1
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5013
ER  - 
@mastersthesis{
author = "Đarmati, Deneš",
year = "2022",
abstract = "U ovom radu će biti opisani algoritmi za analizu geometrijske strukture proteina, njihova
efikasnost i metode optimizacije.",
title = "Izrada alata za izračunavanje dostupne površine proteina u rastvaraču (SASA)",
pages = "1-36",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5013"
}
Đarmati, D.. (2022). Izrada alata za izračunavanje dostupne površine proteina u rastvaraču (SASA). , 1-36.
https://hdl.handle.net/21.15107/rcub_cherry_5013
Đarmati D. Izrada alata za izračunavanje dostupne površine proteina u rastvaraču (SASA). 2022;:1-36.
https://hdl.handle.net/21.15107/rcub_cherry_5013 .
Đarmati, Deneš, "Izrada alata za izračunavanje dostupne površine proteina u rastvaraču (SASA)" (2022):1-36,
https://hdl.handle.net/21.15107/rcub_cherry_5013 .

Diferencijalna laboratorijska dijagnostika mukopolisaharidoza, prikaz kroz pacijente sa mukopolisaharidozom IV-A

Stepanović, Sandra D.

(2022)

TY  - THES
AU  - Stepanović, Sandra D.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5456
AB  - Mukopolisaharidoze (MPS), su podgrupa lizozomalnih bolesti. Uzrokovane su nedostatkom specifičnih enzima, što dovodi do nemogućnosti razgradnje glikozaminoglikana (GAG), koji se potom abnormalno akumuliraju u ćelijama i izlučuju urinom. Preliminarna dijagnoza MPS-a se postavlja izvođenjem prvo neenzimskih testova skrininga, Beri test, test kiselog zamućenja, spektrofotometrijsko određivanje koncentracije GAG u urinu i elektroforeza fragmenata GAG. Kada je određen tip MPS, korišćen je enzimski test sa fluorogenim supstratom za merenje specifične aktivnosti enzima u leukocitima kako bi se potvrdila dijagnoza MPS, ali je određena i aktivnost kontrolnog enzima.
T1  - Diferencijalna laboratorijska dijagnostika mukopolisaharidoza, prikaz kroz pacijente sa mukopolisaharidozom IV-A
SP  - 1
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5456
ER  - 
@mastersthesis{
author = "Stepanović, Sandra D.",
year = "2022",
abstract = "Mukopolisaharidoze (MPS), su podgrupa lizozomalnih bolesti. Uzrokovane su nedostatkom specifičnih enzima, što dovodi do nemogućnosti razgradnje glikozaminoglikana (GAG), koji se potom abnormalno akumuliraju u ćelijama i izlučuju urinom. Preliminarna dijagnoza MPS-a se postavlja izvođenjem prvo neenzimskih testova skrininga, Beri test, test kiselog zamućenja, spektrofotometrijsko određivanje koncentracije GAG u urinu i elektroforeza fragmenata GAG. Kada je određen tip MPS, korišćen je enzimski test sa fluorogenim supstratom za merenje specifične aktivnosti enzima u leukocitima kako bi se potvrdila dijagnoza MPS, ali je određena i aktivnost kontrolnog enzima.",
title = "Diferencijalna laboratorijska dijagnostika mukopolisaharidoza, prikaz kroz pacijente sa mukopolisaharidozom IV-A",
pages = "1-64",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5456"
}
Stepanović, S. D.. (2022). Diferencijalna laboratorijska dijagnostika mukopolisaharidoza, prikaz kroz pacijente sa mukopolisaharidozom IV-A. , 1-64.
https://hdl.handle.net/21.15107/rcub_cherry_5456
Stepanović SD. Diferencijalna laboratorijska dijagnostika mukopolisaharidoza, prikaz kroz pacijente sa mukopolisaharidozom IV-A. 2022;:1-64.
https://hdl.handle.net/21.15107/rcub_cherry_5456 .
Stepanović, Sandra D., "Diferencijalna laboratorijska dijagnostika mukopolisaharidoza, prikaz kroz pacijente sa mukopolisaharidozom IV-A" (2022):1-64,
https://hdl.handle.net/21.15107/rcub_cherry_5456 .

Proizvodnja i prečišćavanje fragmenta N proteina SARS-Cov-2

Bićanin, Maša

(2022)

TY  - THES
AU  - Bićanin, Maša
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5463
AB  - Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov-2) je u periodu od 2 godine uzrokovao smrt miliona ljudi. Brza dijagnostika je prvi front borbe protiv ovog virusa. Razvijanje ELISA tehnika za dijagnostifikovanje, praćenje toka bolesti, ali i efikasnosti vakcina, sada već uveliko dostupnih, su od velikog značaja. Ekspresija nukleokapsida - N proteina, jednog od strukturnih proteina SARS-CoV-2, u bakterijskom sistemu je brza, reproducibilna i jeftina u poređenju sa drugim sistemima, a daje odgovarajući protein kompatibilan za ELISA testove. U ovom radu je eksprimiran fragment N proteina jer je pokazano da ceo N protein daje lažno pozitivne rezultate zbog konzerviranog N - terminalnog N domena. N protein je proizveden u E. coli BL21, a zatim je prečišćen i proverena je njegova i reaktivnost u ELISA testu sa serumima pacijenata koji su preležali ovaj virus i razvili antitela na ovaj strukturni protein.
T1  - Proizvodnja i prečišćavanje fragmenta N proteina SARS-Cov-2
SP  - 2
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5463
ER  - 
@misc{
author = "Bićanin, Maša",
year = "2022",
abstract = "Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov-2) je u periodu od 2 godine uzrokovao smrt miliona ljudi. Brza dijagnostika je prvi front borbe protiv ovog virusa. Razvijanje ELISA tehnika za dijagnostifikovanje, praćenje toka bolesti, ali i efikasnosti vakcina, sada već uveliko dostupnih, su od velikog značaja. Ekspresija nukleokapsida - N proteina, jednog od strukturnih proteina SARS-CoV-2, u bakterijskom sistemu je brza, reproducibilna i jeftina u poređenju sa drugim sistemima, a daje odgovarajući protein kompatibilan za ELISA testove. U ovom radu je eksprimiran fragment N proteina jer je pokazano da ceo N protein daje lažno pozitivne rezultate zbog konzerviranog N - terminalnog N domena. N protein je proizveden u E. coli BL21, a zatim je prečišćen i proverena je njegova i reaktivnost u ELISA testu sa serumima pacijenata koji su preležali ovaj virus i razvili antitela na ovaj strukturni protein.",
title = "Proizvodnja i prečišćavanje fragmenta N proteina SARS-Cov-2",
pages = "2-45",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5463"
}
Bićanin, M.. (2022). Proizvodnja i prečišćavanje fragmenta N proteina SARS-Cov-2. , 2-45.
https://hdl.handle.net/21.15107/rcub_cherry_5463
Bićanin M. Proizvodnja i prečišćavanje fragmenta N proteina SARS-Cov-2. 2022;:2-45.
https://hdl.handle.net/21.15107/rcub_cherry_5463 .
Bićanin, Maša, "Proizvodnja i prečišćavanje fragmenta N proteina SARS-Cov-2" (2022):2-45,
https://hdl.handle.net/21.15107/rcub_cherry_5463 .

Kloniranje, ekspresija i prečišćavanje rekombinantnog Bet v 1l U E. coli

Spasojević, Nađa B.

(2022)

TY  - THES
AU  - Spasojević, Nađa B.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5486
AB  - Zapisi o postojanju alergije datiraju još od vremena drevne Grčke i rimskog carstva, no, ipak, moderno doba proučavanja alergija se beleži 1800-ih godina, opisivanjem polenske groznice 1819. godine., koja je i dalje najzastupljenija vrsta alergija i danas. Kasnije, 1869. godine se pojavljuje prvi kožni test na alergije. Ovaj test je podrazumevao ubacivanje male količine polena u posekotinu na ruci i posmatranje reakcija. Termin „alergija“ prvi put uvodi bečki pedijatar Clemens von Pirquet 1906. koji je uočio da i kod imuniteta i reakcija preosetljivosti antigen izaziva „izmenjenu reaktivnosti“. Vremenom je reč alergija promenila značenje i sada se koristi kao sinonim za reakcije preosetljivosti posredovane imunoglobulinom E (IgE) (1,2).
Danas alergija, ili atopija, podrazumeva stvaranje specifičnih IgE antitela prilikom kontakta sa alergenom, koje je praćeno otpuštanjem medijatora, a potom i pojavom simptoma.
Koncept imunoterapije je uveden 1914. i predstavljao je isto što i danas, a to je poboljšavanje imunog sistema administriranjem malih doza hipoalergenih formi alergena kako bi se potencijalno postigla dezensibilizacija na određeni alergen, a kasnije i tolerancija. Kasnije, 1930. su proizvedeni antihistaminici, lekovi za suzbijanje simptoma alergijskih reakcija. 1948. je uvedeno korišćenje kortikosteroida kod astme i alergijskih reakcija.
T1  - Kloniranje, ekspresija i prečišćavanje rekombinantnog Bet v 1l U E. coli
SP  - 1
EP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5486
ER  - 
@mastersthesis{
author = "Spasojević, Nađa B.",
year = "2022",
abstract = "Zapisi o postojanju alergije datiraju još od vremena drevne Grčke i rimskog carstva, no, ipak, moderno doba proučavanja alergija se beleži 1800-ih godina, opisivanjem polenske groznice 1819. godine., koja je i dalje najzastupljenija vrsta alergija i danas. Kasnije, 1869. godine se pojavljuje prvi kožni test na alergije. Ovaj test je podrazumevao ubacivanje male količine polena u posekotinu na ruci i posmatranje reakcija. Termin „alergija“ prvi put uvodi bečki pedijatar Clemens von Pirquet 1906. koji je uočio da i kod imuniteta i reakcija preosetljivosti antigen izaziva „izmenjenu reaktivnosti“. Vremenom je reč alergija promenila značenje i sada se koristi kao sinonim za reakcije preosetljivosti posredovane imunoglobulinom E (IgE) (1,2).
Danas alergija, ili atopija, podrazumeva stvaranje specifičnih IgE antitela prilikom kontakta sa alergenom, koje je praćeno otpuštanjem medijatora, a potom i pojavom simptoma.
Koncept imunoterapije je uveden 1914. i predstavljao je isto što i danas, a to je poboljšavanje imunog sistema administriranjem malih doza hipoalergenih formi alergena kako bi se potencijalno postigla dezensibilizacija na određeni alergen, a kasnije i tolerancija. Kasnije, 1930. su proizvedeni antihistaminici, lekovi za suzbijanje simptoma alergijskih reakcija. 1948. je uvedeno korišćenje kortikosteroida kod astme i alergijskih reakcija.",
title = "Kloniranje, ekspresija i prečišćavanje rekombinantnog Bet v 1l U E. coli",
pages = "1-55",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5486"
}
Spasojević, N. B.. (2022). Kloniranje, ekspresija i prečišćavanje rekombinantnog Bet v 1l U E. coli. , 1-55.
https://hdl.handle.net/21.15107/rcub_cherry_5486
Spasojević NB. Kloniranje, ekspresija i prečišćavanje rekombinantnog Bet v 1l U E. coli. 2022;:1-55.
https://hdl.handle.net/21.15107/rcub_cherry_5486 .
Spasojević, Nađa B., "Kloniranje, ekspresija i prečišćavanje rekombinantnog Bet v 1l U E. coli" (2022):1-55,
https://hdl.handle.net/21.15107/rcub_cherry_5486 .

Kloniranje i ekspresija rekombinantne himere Bet v1.001-BanLecH84T u Escherichia coli

Cvetković, Katarina I.

(2022)

TY  - THES
AU  - Cvetković, Katarina I.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5487
AB  - Respiratorna alergijska bolest predstavlja značajan zdravstveni problem. Najčešća je hronična
bolest kako kod odraslih tako i kod dece. Kliničke manifestacije alergijske bolesti uključuju
astmu, rinitis, konjuktivitis, anafilaksu, ekcem i urtikartiju. Prema statistikama Svetske
zdravstvene organizacije (SZO), milioni ljudi u svetu boluju od rinitisa i procenjuje se da 235
miliona ljudi ima astmu. Evropska akademija za alergiju i kliničku imunologiju predviđa da će u
narednih nekoliko decenija više od polovine evropske populacije će imati neku vrstu alergije.
Alergen specifična imunoterapija se bavi osnovom alergijske bolesti. Utiče na imunološke
mehanizme i rezultira indukcijom imunološke tolerancije. Indukovana tolerancija podrazumeva
modifikaciju bolesti, čiji su efekti trajno smanjenje simptoma nakon završetka lečanja i
sprečavanje progresije bolesti.
T1  - Kloniranje i ekspresija rekombinantne himere Bet v1.001-BanLecH84T u Escherichia coli
SP  - 2
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5487
ER  - 
@mastersthesis{
author = "Cvetković, Katarina I.",
year = "2022",
abstract = "Respiratorna alergijska bolest predstavlja značajan zdravstveni problem. Najčešća je hronična
bolest kako kod odraslih tako i kod dece. Kliničke manifestacije alergijske bolesti uključuju
astmu, rinitis, konjuktivitis, anafilaksu, ekcem i urtikartiju. Prema statistikama Svetske
zdravstvene organizacije (SZO), milioni ljudi u svetu boluju od rinitisa i procenjuje se da 235
miliona ljudi ima astmu. Evropska akademija za alergiju i kliničku imunologiju predviđa da će u
narednih nekoliko decenija više od polovine evropske populacije će imati neku vrstu alergije.
Alergen specifična imunoterapija se bavi osnovom alergijske bolesti. Utiče na imunološke
mehanizme i rezultira indukcijom imunološke tolerancije. Indukovana tolerancija podrazumeva
modifikaciju bolesti, čiji su efekti trajno smanjenje simptoma nakon završetka lečanja i
sprečavanje progresije bolesti.",
title = "Kloniranje i ekspresija rekombinantne himere Bet v1.001-BanLecH84T u Escherichia coli",
pages = "2-34",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5487"
}
Cvetković, K. I.. (2022). Kloniranje i ekspresija rekombinantne himere Bet v1.001-BanLecH84T u Escherichia coli. , 2-34.
https://hdl.handle.net/21.15107/rcub_cherry_5487
Cvetković KI. Kloniranje i ekspresija rekombinantne himere Bet v1.001-BanLecH84T u Escherichia coli. 2022;:2-34.
https://hdl.handle.net/21.15107/rcub_cherry_5487 .
Cvetković, Katarina I., "Kloniranje i ekspresija rekombinantne himere Bet v1.001-BanLecH84T u Escherichia coli" (2022):2-34,
https://hdl.handle.net/21.15107/rcub_cherry_5487 .

Optimizacija enzimskog spektrofotometrijskog eseja za kvantifikaciju L-fenilalanina

Maksimović, Dušan

(2022)

TY  - THES
AU  - Maksimović, Dušan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5550
AB  - Fenilketonurija (PKU) predstavlja jednu od najčešćih naslednih poremećaja metabolizma aminokiselina. PKU je poremećaj metabolizma fenilalanina koji se prenosi autozomno recesivnim putem. Javlja se kao posledica mutacije enzima jetre fenilalanin hidroksilaze, koja katalizuje hidroksilaciju fenilalanina u tirozin. Nedostatak ovog enzima dovodi do nagomilavanja fenilalanina u organizmu, što za posledicu ima niz neurotoksičnih efekata kao i razvoj mentalne retardacije. Razvojem neonatalnih skrining testova širom sveta su omogućile blagovremeno uspostavljanje dijagnoze i tretmana naslednih metaboličkih bolesti. Metode koje se koriste za neonatalni skrining su Gutrijeva metoda, fluorimetrijski testovi, enzimski testovi, HPLC i masena spektrometrija. Ubrzan razvoj biohemije i biotehnologije dovode do boljeg razumevanja molekularne osnove PKU, kao i potreba za poboljšanjem mogućnosti dijagnoze i razvoja novih efikasnijih metoda za utvrđivanje koncentracije fenilalanina.
U fokusu ovog završnog rada je rekombinantna L-fenilalanin dehidrogenaza (PheDH) bakterijskog porekla. Kako je ovaj enzim obećavajući kandidat za razvoj biosenzora za monitoring fenilketonurije, cilj rada je bio proizvodnja rekombinantnog enzima i optimizacija spektrofotometrijskog eseja radi određivanja koncentracije L-Phe u različitim uzorcima.
T1  - Optimizacija enzimskog spektrofotometrijskog eseja za kvantifikaciju L-fenilalanina
SP  - 1
EP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5550
ER  - 
@misc{
author = "Maksimović, Dušan",
year = "2022",
abstract = "Fenilketonurija (PKU) predstavlja jednu od najčešćih naslednih poremećaja metabolizma aminokiselina. PKU je poremećaj metabolizma fenilalanina koji se prenosi autozomno recesivnim putem. Javlja se kao posledica mutacije enzima jetre fenilalanin hidroksilaze, koja katalizuje hidroksilaciju fenilalanina u tirozin. Nedostatak ovog enzima dovodi do nagomilavanja fenilalanina u organizmu, što za posledicu ima niz neurotoksičnih efekata kao i razvoj mentalne retardacije. Razvojem neonatalnih skrining testova širom sveta su omogućile blagovremeno uspostavljanje dijagnoze i tretmana naslednih metaboličkih bolesti. Metode koje se koriste za neonatalni skrining su Gutrijeva metoda, fluorimetrijski testovi, enzimski testovi, HPLC i masena spektrometrija. Ubrzan razvoj biohemije i biotehnologije dovode do boljeg razumevanja molekularne osnove PKU, kao i potreba za poboljšanjem mogućnosti dijagnoze i razvoja novih efikasnijih metoda za utvrđivanje koncentracije fenilalanina.
U fokusu ovog završnog rada je rekombinantna L-fenilalanin dehidrogenaza (PheDH) bakterijskog porekla. Kako je ovaj enzim obećavajući kandidat za razvoj biosenzora za monitoring fenilketonurije, cilj rada je bio proizvodnja rekombinantnog enzima i optimizacija spektrofotometrijskog eseja radi određivanja koncentracije L-Phe u različitim uzorcima.",
title = "Optimizacija enzimskog spektrofotometrijskog eseja za kvantifikaciju L-fenilalanina",
pages = "1-40",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5550"
}
Maksimović, D.. (2022). Optimizacija enzimskog spektrofotometrijskog eseja za kvantifikaciju L-fenilalanina. , 1-40.
https://hdl.handle.net/21.15107/rcub_cherry_5550
Maksimović D. Optimizacija enzimskog spektrofotometrijskog eseja za kvantifikaciju L-fenilalanina. 2022;:1-40.
https://hdl.handle.net/21.15107/rcub_cherry_5550 .
Maksimović, Dušan, "Optimizacija enzimskog spektrofotometrijskog eseja za kvantifikaciju L-fenilalanina" (2022):1-40,
https://hdl.handle.net/21.15107/rcub_cherry_5550 .

Poređenje različitih metoda prečišćavanja F(ab’)2 fragmenta antitela

Filipović, Goran

(2022)

TY  - THES
AU  - Filipović, Goran
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5577
AB  - Glavni cilj ovog rada bio je ispitvanje mogućnosti prečišćavanja F(ab)2 fragmenata imunoglobilina iz nativne plazme različitim tehnikama, uz identifikovanje svih kritičnih faza/koraka i procesnih parametara, koji mogu da utiču na kvalitet i prinos proizvoda.
T1  - Poređenje različitih metoda prečišćavanja F(ab’)2 fragmenta antitela
SP  - 1
EP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5577
ER  - 
@mastersthesis{
author = "Filipović, Goran",
year = "2022",
abstract = "Glavni cilj ovog rada bio je ispitvanje mogućnosti prečišćavanja F(ab)2 fragmenata imunoglobilina iz nativne plazme različitim tehnikama, uz identifikovanje svih kritičnih faza/koraka i procesnih parametara, koji mogu da utiču na kvalitet i prinos proizvoda.",
title = "Poređenje različitih metoda prečišćavanja F(ab’)2 fragmenta antitela",
pages = "1-57",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5577"
}
Filipović, G.. (2022). Poređenje različitih metoda prečišćavanja F(ab’)2 fragmenta antitela. , 1-57.
https://hdl.handle.net/21.15107/rcub_cherry_5577
Filipović G. Poređenje različitih metoda prečišćavanja F(ab’)2 fragmenta antitela. 2022;:1-57.
https://hdl.handle.net/21.15107/rcub_cherry_5577 .
Filipović, Goran, "Poređenje različitih metoda prečišćavanja F(ab’)2 fragmenta antitela" (2022):1-57,
https://hdl.handle.net/21.15107/rcub_cherry_5577 .

Primena rekombinantnih proteina SARS-CoV-2 virusa u analizi virus-specifičnog imunskog odgovora

Anđelović, Ivana

(2022)

TY  - THES
AU  - Anđelović, Ivana
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5578
AB  - Nakon skoro tri godine od pojave SARS-CoV-2 virusa u kineskom gradu Vuhanu, koji se veoma brzo proširio i na ostatak sveta, izazivajući najgoru pandemiju još od kraja Prvog svetskog rata i tzv. Španske groznice, zahvaljujući biomedicinskim istraživanjima, danas raspolažemo širokim spektrom vakcina protiv COVID-19. Vakcine protiv COVID-19 uključuju tradicionalne - inaktivirane vakcine, kao i vektorske vakcine i mRNA vakcine koje predstavljaju novu generaciju vakcina i platformu za mnoga istraživanja i proizvodnju drugih proizvoda zasnovanih na mRNA tehnologiji. Takođe, raspolažemo i brojnim serološkim testovima, kako za kvalitativnu tako i za semi-kvantitativnu i kvantitativnu analizu. Navedeni testovi u kratkom vremenskom roku daju rezultate koji mogu biti iskorišćeni u dijagnostici, praćenju kontakata i za epidemiološke studije.
Ova studija je rađena u Institutu za virusologiju, vakcine i serume "Torlak", u Odseku za naučno-istraživački rad i ima za cilj da pokaže mogućnost primene antigena SARS-CoV-2 virusa, proizvedenih rekombinantnom tehnologijom, u praćenju SARS-CoV-2 specifičnog imunskog odgovora. U urađenim istraživanjima korišćen je receptor vezujući domen (engl. receptor-binding domain, RBD) spike (S) proteina SARS-CoV-2.
In house ELISA test za praćenje humoralnog imunskog odgovora na RBD SARS-CoV-2 virusa poređen je sa komercijalno dostupnim ELISA test-om za detekciju i kvantifikaciju IgG antitela na SARS-CoV-2. Pored toga, rezultate ova dva serološka testa smo uporedili sa rezultatima dobijenim u testu neutralizacije, koji služi kao zlatni standard za određivanje funkcionalne sposobnosti antitela da neutrališu SARS-CoV-2 virus. Povezivanje neutralizacione aktivnosti sa rezultatom serološkog testa pomaže u boljoj karakterizaciji njihove dijagnostičke vrednosti, a to može biti iskorišćeno u cilju postavljanja dijagnoze, potvrđivanja zaštite od infekcije, sprovođenja epidemioloških istraživanja i slično.
Pored analiziranja koncentracije anti-SARS-CoV-2 IgG antitela, koja su deo humoralnog imunog odgovora, deo studije se bavio i ćelijskim imunitetom - stimulacijom T ćelija strukturnim antigenima SARS-CoV-2 virusa.
T1  - Primena rekombinantnih proteina SARS-CoV-2 virusa u analizi virus-specifičnog imunskog odgovora
SP  - 1
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5578
ER  - 
@mastersthesis{
author = "Anđelović, Ivana",
year = "2022",
abstract = "Nakon skoro tri godine od pojave SARS-CoV-2 virusa u kineskom gradu Vuhanu, koji se veoma brzo proširio i na ostatak sveta, izazivajući najgoru pandemiju još od kraja Prvog svetskog rata i tzv. Španske groznice, zahvaljujući biomedicinskim istraživanjima, danas raspolažemo širokim spektrom vakcina protiv COVID-19. Vakcine protiv COVID-19 uključuju tradicionalne - inaktivirane vakcine, kao i vektorske vakcine i mRNA vakcine koje predstavljaju novu generaciju vakcina i platformu za mnoga istraživanja i proizvodnju drugih proizvoda zasnovanih na mRNA tehnologiji. Takođe, raspolažemo i brojnim serološkim testovima, kako za kvalitativnu tako i za semi-kvantitativnu i kvantitativnu analizu. Navedeni testovi u kratkom vremenskom roku daju rezultate koji mogu biti iskorišćeni u dijagnostici, praćenju kontakata i za epidemiološke studije.
Ova studija je rađena u Institutu za virusologiju, vakcine i serume "Torlak", u Odseku za naučno-istraživački rad i ima za cilj da pokaže mogućnost primene antigena SARS-CoV-2 virusa, proizvedenih rekombinantnom tehnologijom, u praćenju SARS-CoV-2 specifičnog imunskog odgovora. U urađenim istraživanjima korišćen je receptor vezujući domen (engl. receptor-binding domain, RBD) spike (S) proteina SARS-CoV-2.
In house ELISA test za praćenje humoralnog imunskog odgovora na RBD SARS-CoV-2 virusa poređen je sa komercijalno dostupnim ELISA test-om za detekciju i kvantifikaciju IgG antitela na SARS-CoV-2. Pored toga, rezultate ova dva serološka testa smo uporedili sa rezultatima dobijenim u testu neutralizacije, koji služi kao zlatni standard za određivanje funkcionalne sposobnosti antitela da neutrališu SARS-CoV-2 virus. Povezivanje neutralizacione aktivnosti sa rezultatom serološkog testa pomaže u boljoj karakterizaciji njihove dijagnostičke vrednosti, a to može biti iskorišćeno u cilju postavljanja dijagnoze, potvrđivanja zaštite od infekcije, sprovođenja epidemioloških istraživanja i slično.
Pored analiziranja koncentracije anti-SARS-CoV-2 IgG antitela, koja su deo humoralnog imunog odgovora, deo studije se bavio i ćelijskim imunitetom - stimulacijom T ćelija strukturnim antigenima SARS-CoV-2 virusa.",
title = "Primena rekombinantnih proteina SARS-CoV-2 virusa u analizi virus-specifičnog imunskog odgovora",
pages = "1-36",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5578"
}
Anđelović, I.. (2022). Primena rekombinantnih proteina SARS-CoV-2 virusa u analizi virus-specifičnog imunskog odgovora. , 1-36.
https://hdl.handle.net/21.15107/rcub_cherry_5578
Anđelović I. Primena rekombinantnih proteina SARS-CoV-2 virusa u analizi virus-specifičnog imunskog odgovora. 2022;:1-36.
https://hdl.handle.net/21.15107/rcub_cherry_5578 .
Anđelović, Ivana, "Primena rekombinantnih proteina SARS-CoV-2 virusa u analizi virus-specifičnog imunskog odgovora" (2022):1-36,
https://hdl.handle.net/21.15107/rcub_cherry_5578 .

La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells

Knežević, Sara; Ognjanović, Miloš; Stanković, Vesna; Zlatanova, Milena; Nešić, Andrijana; Gavrović-Jankulović, Marija; Stanković, Dalibor

(MDPI, 2022)

TY  - JOUR
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5622
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells
VL  - 12
IS  - 9
SP  - 705
DO  - 10.3390/bios12090705
ER  - 
@article{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells",
volume = "12",
number = "9",
pages = "705",
doi = "10.3390/bios12090705"
}
Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors
MDPI., 12(9), 705.
https://doi.org/10.3390/bios12090705
Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić A, Gavrović-Jankulović M, Stanković D. La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors. 2022;12(9):705.
doi:10.3390/bios12090705 .
Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana, Gavrović-Jankulović, Marija, Stanković, Dalibor, "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells" in Biosensors, 12, no. 9 (2022):705,
https://doi.org/10.3390/bios12090705 . .
1

A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels

Nešić, Andrijana; Stojsavljević, Aleksandar; Jagodić, Jovana; Čavić, Milena; Stefanović, Aleksandra; Manojlović, Dragan D.; Gavrović-Jankulović, Marija

(Elsevier, 2022)

TY  - JOUR
AU  - Nešić, Andrijana
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Čavić, Milena
AU  - Stefanović, Aleksandra
AU  - Manojlović, Dragan D.
AU  - Gavrović-Jankulović, Marija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5636
AB  - Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels
VL  - 74
SP  - 127079
DO  - 10.1016/j.jtemb.2022.127079
ER  - 
@article{
author = "Nešić, Andrijana and Stojsavljević, Aleksandar and Jagodić, Jovana and Čavić, Milena and Stefanović, Aleksandra and Manojlović, Dragan D. and Gavrović-Jankulović, Marija",
year = "2022",
abstract = "Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels",
volume = "74",
pages = "127079",
doi = "10.1016/j.jtemb.2022.127079"
}
Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D. D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology
Elsevier., 74, 127079.
https://doi.org/10.1016/j.jtemb.2022.127079
Nešić A, Stojsavljević A, Jagodić J, Čavić M, Stefanović A, Manojlović DD, Gavrović-Jankulović M. A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology. 2022;74:127079.
doi:10.1016/j.jtemb.2022.127079 .
Nešić, Andrijana, Stojsavljević, Aleksandar, Jagodić, Jovana, Čavić, Milena, Stefanović, Aleksandra, Manojlović, Dragan D., Gavrović-Jankulović, Marija, "A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels" in Journal of Trace Elements in Medicine and Biology, 74 (2022):127079,
https://doi.org/10.1016/j.jtemb.2022.127079 . .

Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705

Knežević, Sara; Ognjanović, Miloš; Stanković, Vesna; Zlatanova, Milena; Nešić, Andrijana; Gavrović-Jankulović, Marija; Stanković, Dalibor

(MDPI, 2022)

TY  - DATA
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5632
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705
VL  - 12
IS  - 9
SP  - 705
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5632
ER  - 
@misc{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705",
volume = "12",
number = "9",
pages = "705",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5632"
}
Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors
MDPI., 12(9), 705.
https://hdl.handle.net/21.15107/rcub_cherry_5632
Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić A, Gavrović-Jankulović M, Stanković D. Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors. 2022;12(9):705.
https://hdl.handle.net/21.15107/rcub_cherry_5632 .
Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana, Gavrović-Jankulović, Marija, Stanković, Dalibor, "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705" in Biosensors, 12, no. 9 (2022):705,
https://hdl.handle.net/21.15107/rcub_cherry_5632 .

Upotreba hidrogelova dobijenih modifikacijom polisaharida fenolnim jedinjenjima za imobilizaciju ćelija i biokatalizatora

Popović Kokar, Nikolina

(Универзитет у Београду, Хемијски факултет, 2022)

TY  - THES
AU  - Popović Kokar, Nikolina
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5641
AB  - Hidrogelovi predstavljaju polimerne mreže sa hidrofilnim osobinama, sposobne da apsorbuju velike količine vode, uz povećanje svoje zapremine. Geliranje se može izvesti fizičkim ili hemijskim tipovima umrežavanja polimernih lanaca, koji mogu da vode poreklo od prirodnih i sintetičkih polimera. U oblastima biomedicine, najzastupljeniji su hidrogelovi dobijeni od prirodnih polimera, kao što su polisaharidi i njihovi derivati.Cilj ovog rada je bio modifikovati različite polisaharide (alginat, pektin, karboksimetilceluloza) fenolnim jedinjenjima (tiramin, tirozin, dopamin, L-DOPA), sa ciljem dobijanja polimera sposobnih da grade hidrogelove. Uspešnost modifikacije je okarakterisana UV-Vis, FTIR i 1H NMR spektroskopskim metodama. Ispitana je njihova sposobnost geliranja, nakon čega su oni sa najpogodnijim karakteristikama primenjeni u različitim oblastima.Dopaminski derivati alginata su korišćeni za enkapsulaciju ćelijskih zidova sa enzimom lakazom, dobijenih lizom ćelija Saccharomyces cerevisiae sa prethodno eksprimiranom lakazom na površini ćelije. Dobijeni ćelijski zidovi sa lakazom su pokazali 300 puta veću enzimsku aktivnost u odnosu na nelizirane ćelije. Uspešno je urađena imobilizacija u kalcijum-dopamin-alginatnim kuglicama, koje su pokazale prednost u odnosu na kuglice sa nativnim alginatom. Ovako dobijeni biokatalizatori su korišćeni za dekolorizaciju tekstilnih boja Amido Black 10B, Reactive Black 5, Evans Blue, i Remazol Brilliant Blue. Nakon 10 ciklusa dekolorizacije od po 48 sati, efikasnost dekolorizacije za boje Evans Blue i Amido Black 10 B je iznosila 90 % i 61 %, navedenim redom.Lakaza iz Streptomyces cyaneus je takođe eksprimirana u E. coli, izolovana i prečišćena. Izolovana lakaza je imobilizovana u dopamin-pektinskim mikrokuglicama, metodom enzimske polimerizacije u emulziji. Imobilizovana lakaza je pokazala povećanu termalnu i pH stabilnost u odnosu na slobodan enzim, kao i sposobnost dekolorizacije boja Amido Black 10B, Reactive Black 5 i Evans Blue. U uzastopnim ciklusima dekolorizacije boja je ovaj biokatalizator pokazao najviše potencijala za degradaciju boja Amido Black 10B i Reactive Black 5.Sintetisani dopaminski derivati karboksimetilceluloze i alginata su korišćeni za proizvodnju nanovlakana elektrospining metodom. Dobijena nanovlakna su dodatno umrežavana i korišćena kao podloga za gajenje ćelija fibroblasta (MRC-5). Dopamin-alginatna nanovlakna su se pokazala kao dobar materijal za gajenje ćelija, što je pokazano snimanjem SEM-a i MTT esejem, iz čega bi moglo da se zaključi da su adhezija i proliferacija ćelija moguće na ovim nanovlaknima i da su ona potencijalno dobar materijal za tkivni inženjering.
AB  - Hydrogels are polymer networks, which have hydrophilic properties and could absorb large amount of water, increasing their volume. They could form gels either by physical or chemical cross-linking of polymer chains and could be derived from natural and synthetic polymers.The aim of this study was to modificate diverse polysaccharides (alginate, pectin, and carboxymethylcellulose) with phenol compounds (tyramine, tyrosine, dopamine, L-DOPA), in order to obtain polymers which could form hydrogels. The success of modification was confirmed by UV-Vis, FTIR and 1H NMR spectroscopy. Their gelling ability was investigated, afterward polymers with the most suitable characteristics were applied in different areas.Dopamine alginate derivatives were used to encapsulate cell walls with the enzyme laccase, obtained by lysing cells of Saccharomyces cerevisiae, with previously expressed laccase on the cell surface. The obtained cell wall laccase has shown 300 times higher enzyme activity, compared to the whole cells. Immobilization was successfully performed in calcium-dopamine-alginate beads, which showed an advantage compared to beads with native alginate. Thus obtained biocatalyst were used for decolorization of textile dyes Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue. After ten cycles of repeated use, decolourization efficiency for dyes Evans Blue and Amido Black 10B was 90 % and 61 %, respectively.Laccase from Streptomyces cyaneus was also expressed in E. coli, isolated and purified. The isolated laccase was immobilized in dopamine-pectin microbeads, using emulsion-based enzymatic crosslinking polymerization. The immobilized laccase showed improved thermal and pH stability in comparison to the free enzyme, and ability of decolorization dyes Amido Black 10B, Reactive Black 5 and Evans Blue. In successive cycles of dyes decolorization, this biocatalyst showed the greatest potential for degradation of Amido Black 10B and Reactive Black 5.The synthesized dopamine derivatives of carboxymethylcellulose and alginate were used for the production of nanofibers by the method of electrospinning. The obtained nanofibers were additionaly crosslinked and used as a scaffold for fibroblast cells (MRC-5) cultivation. Dopamin-alginate nanofibers have been shown as a good material for cell cultivation, as shown by SEMimaging and MTT assay. It could be concluded that cell adhesion and proliferation are possible on these nanofibers and they are potentially good material for tissue engineering.
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Upotreba hidrogelova dobijenih modifikacijom polisaharida fenolnim jedinjenjima za imobilizaciju ćelija i biokatalizatora
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5641
ER  - 
@phdthesis{
author = "Popović Kokar, Nikolina",
year = "2022",
abstract = "Hidrogelovi predstavljaju polimerne mreže sa hidrofilnim osobinama, sposobne da apsorbuju velike količine vode, uz povećanje svoje zapremine. Geliranje se može izvesti fizičkim ili hemijskim tipovima umrežavanja polimernih lanaca, koji mogu da vode poreklo od prirodnih i sintetičkih polimera. U oblastima biomedicine, najzastupljeniji su hidrogelovi dobijeni od prirodnih polimera, kao što su polisaharidi i njihovi derivati.Cilj ovog rada je bio modifikovati različite polisaharide (alginat, pektin, karboksimetilceluloza) fenolnim jedinjenjima (tiramin, tirozin, dopamin, L-DOPA), sa ciljem dobijanja polimera sposobnih da grade hidrogelove. Uspešnost modifikacije je okarakterisana UV-Vis, FTIR i 1H NMR spektroskopskim metodama. Ispitana je njihova sposobnost geliranja, nakon čega su oni sa najpogodnijim karakteristikama primenjeni u različitim oblastima.Dopaminski derivati alginata su korišćeni za enkapsulaciju ćelijskih zidova sa enzimom lakazom, dobijenih lizom ćelija Saccharomyces cerevisiae sa prethodno eksprimiranom lakazom na površini ćelije. Dobijeni ćelijski zidovi sa lakazom su pokazali 300 puta veću enzimsku aktivnost u odnosu na nelizirane ćelije. Uspešno je urađena imobilizacija u kalcijum-dopamin-alginatnim kuglicama, koje su pokazale prednost u odnosu na kuglice sa nativnim alginatom. Ovako dobijeni biokatalizatori su korišćeni za dekolorizaciju tekstilnih boja Amido Black 10B, Reactive Black 5, Evans Blue, i Remazol Brilliant Blue. Nakon 10 ciklusa dekolorizacije od po 48 sati, efikasnost dekolorizacije za boje Evans Blue i Amido Black 10 B je iznosila 90 % i 61 %, navedenim redom.Lakaza iz Streptomyces cyaneus je takođe eksprimirana u E. coli, izolovana i prečišćena. Izolovana lakaza je imobilizovana u dopamin-pektinskim mikrokuglicama, metodom enzimske polimerizacije u emulziji. Imobilizovana lakaza je pokazala povećanu termalnu i pH stabilnost u odnosu na slobodan enzim, kao i sposobnost dekolorizacije boja Amido Black 10B, Reactive Black 5 i Evans Blue. U uzastopnim ciklusima dekolorizacije boja je ovaj biokatalizator pokazao najviše potencijala za degradaciju boja Amido Black 10B i Reactive Black 5.Sintetisani dopaminski derivati karboksimetilceluloze i alginata su korišćeni za proizvodnju nanovlakana elektrospining metodom. Dobijena nanovlakna su dodatno umrežavana i korišćena kao podloga za gajenje ćelija fibroblasta (MRC-5). Dopamin-alginatna nanovlakna su se pokazala kao dobar materijal za gajenje ćelija, što je pokazano snimanjem SEM-a i MTT esejem, iz čega bi moglo da se zaključi da su adhezija i proliferacija ćelija moguće na ovim nanovlaknima i da su ona potencijalno dobar materijal za tkivni inženjering., Hydrogels are polymer networks, which have hydrophilic properties and could absorb large amount of water, increasing their volume. They could form gels either by physical or chemical cross-linking of polymer chains and could be derived from natural and synthetic polymers.The aim of this study was to modificate diverse polysaccharides (alginate, pectin, and carboxymethylcellulose) with phenol compounds (tyramine, tyrosine, dopamine, L-DOPA), in order to obtain polymers which could form hydrogels. The success of modification was confirmed by UV-Vis, FTIR and 1H NMR spectroscopy. Their gelling ability was investigated, afterward polymers with the most suitable characteristics were applied in different areas.Dopamine alginate derivatives were used to encapsulate cell walls with the enzyme laccase, obtained by lysing cells of Saccharomyces cerevisiae, with previously expressed laccase on the cell surface. The obtained cell wall laccase has shown 300 times higher enzyme activity, compared to the whole cells. Immobilization was successfully performed in calcium-dopamine-alginate beads, which showed an advantage compared to beads with native alginate. Thus obtained biocatalyst were used for decolorization of textile dyes Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue. After ten cycles of repeated use, decolourization efficiency for dyes Evans Blue and Amido Black 10B was 90 % and 61 %, respectively.Laccase from Streptomyces cyaneus was also expressed in E. coli, isolated and purified. The isolated laccase was immobilized in dopamine-pectin microbeads, using emulsion-based enzymatic crosslinking polymerization. The immobilized laccase showed improved thermal and pH stability in comparison to the free enzyme, and ability of decolorization dyes Amido Black 10B, Reactive Black 5 and Evans Blue. In successive cycles of dyes decolorization, this biocatalyst showed the greatest potential for degradation of Amido Black 10B and Reactive Black 5.The synthesized dopamine derivatives of carboxymethylcellulose and alginate were used for the production of nanofibers by the method of electrospinning. The obtained nanofibers were additionaly crosslinked and used as a scaffold for fibroblast cells (MRC-5) cultivation. Dopamin-alginate nanofibers have been shown as a good material for cell cultivation, as shown by SEMimaging and MTT assay. It could be concluded that cell adhesion and proliferation are possible on these nanofibers and they are potentially good material for tissue engineering.",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Upotreba hidrogelova dobijenih modifikacijom polisaharida fenolnim jedinjenjima za imobilizaciju ćelija i biokatalizatora",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5641"
}
Popović Kokar, N.. (2022). Upotreba hidrogelova dobijenih modifikacijom polisaharida fenolnim jedinjenjima za imobilizaciju ćelija i biokatalizatora. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_cherry_5641
Popović Kokar N. Upotreba hidrogelova dobijenih modifikacijom polisaharida fenolnim jedinjenjima za imobilizaciju ćelija i biokatalizatora. in Универзитет у Београду. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5641 .
Popović Kokar, Nikolina, "Upotreba hidrogelova dobijenih modifikacijom polisaharida fenolnim jedinjenjima za imobilizaciju ćelija i biokatalizatora" in Универзитет у Београду (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5641 .

Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079

Nešić, Andrijana; Stojsavljević, Aleksandar; Jagodić, Jovana; Čavić, Milena; Stefanović, Aleksandra; Manojlović, Dragan D.; Gavrović-Jankulović, Marija

(Elsevier, 2022)

TY  - DATA
AU  - Nešić, Andrijana
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Čavić, Milena
AU  - Stefanović, Aleksandra
AU  - Manojlović, Dragan D.
AU  - Gavrović-Jankulović, Marija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5658
AB  - Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079
VL  - 74
SP  - 127079
DO  - 10.1016/j.jtemb.2022.127079
ER  - 
@misc{
author = "Nešić, Andrijana and Stojsavljević, Aleksandar and Jagodić, Jovana and Čavić, Milena and Stefanović, Aleksandra and Manojlović, Dragan D. and Gavrović-Jankulović, Marija",
year = "2022",
abstract = "Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079",
volume = "74",
pages = "127079",
doi = "10.1016/j.jtemb.2022.127079"
}
Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D. D.,& Gavrović-Jankulović, M.. (2022). Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079. in Journal of Trace Elements in Medicine and Biology
Elsevier., 74, 127079.
https://doi.org/10.1016/j.jtemb.2022.127079
Nešić A, Stojsavljević A, Jagodić J, Čavić M, Stefanović A, Manojlović DD, Gavrović-Jankulović M. Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079. in Journal of Trace Elements in Medicine and Biology. 2022;74:127079.
doi:10.1016/j.jtemb.2022.127079 .
Nešić, Andrijana, Stojsavljević, Aleksandar, Jagodić, Jovana, Čavić, Milena, Stefanović, Aleksandra, Manojlović, Dragan D., Gavrović-Jankulović, Marija, "Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079" in Journal of Trace Elements in Medicine and Biology, 74 (2022):127079,
https://doi.org/10.1016/j.jtemb.2022.127079 . .

Određivanje sadržaja toksičnih i esencijalnih elemenata u tkivu placente

Ivanović, Katarina

(2022)

TY  - THES
AU  - Ivanović, Katarina
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4872
AB  - Izloženost određenim elementima prepoznata je kao faktor rizika po zdravlje ljudi, a uzrok može biti izlaganje toksičnim elementima kroz ishranu, vazduh, vodu za piće, konzumiranjem cigareta i profesionalnu izloženost. Posebnu pažnju treba posvetiti izloženosti elementima kod trudnica, zbog razvoja fetusa. Ukoliko dođe do prekomernog izlaganja polutantima iz životne sredine, poput elemenata u tragovima, oni mogu uticati štetno na zdravlje i razvoj fetusa.
Placenta predstavlja vezu između majke i fetusa, posreduje u prenosu hranljivih materija i sprečava prodiranje toksičnih elemenata. Novorođenčad zbog nerazvijenog imunskog sistema i razvoj fetalnih organa ima veće apsorpcije u odnosu na odrasle. U okviru ovog istraživanja iz tkiva placenti, ispitani su sadržaji toksičnih elemenata poput arsena, olova, aluminijuma, kadmijuma, nikla i esencijalni elementi koji su od značaja za fetus selen, cink, bakar, mangan i hrom.
Određivanje elemenata u tragovima u kliničkim uzorcima od velike su važnosti za veliki broj kliničkih ispitivanja. Propustljivost placente za esencijalne i neesencijalne elemente u tragovima su nedovoljno proučeni. Poslednjih decenija proučavana je izloženost elementima u tragovima in utero, iako je većina radova razmatrala samo nekoliko metala u tragovima ili nedovoljan broj uzoraka placente. U ovom radu smo imali za cilj da odredimo sadržaj 10 elemenata u tragovima u tkivima placenti. Dalji cilj bio je da se predloži normalni (referentni) opseg za svaki određivani element u tragovima.
Pored toga, imali smo za cilj da uporedimo naše rezultate sa drugim grupama stanovništva širom sveta, da se istakne značaj elemenata na zdravlje placente.Postupak određivanja vrednosti ovih elemenata u tragovima rađen je ICP-MS metodom.
T1  - Određivanje sadržaja toksičnih i esencijalnih elemenata u tkivu placente
SP  - 1
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4872
ER  - 
@misc{
author = "Ivanović, Katarina",
year = "2022",
abstract = "Izloženost određenim elementima prepoznata je kao faktor rizika po zdravlje ljudi, a uzrok može biti izlaganje toksičnim elementima kroz ishranu, vazduh, vodu za piće, konzumiranjem cigareta i profesionalnu izloženost. Posebnu pažnju treba posvetiti izloženosti elementima kod trudnica, zbog razvoja fetusa. Ukoliko dođe do prekomernog izlaganja polutantima iz životne sredine, poput elemenata u tragovima, oni mogu uticati štetno na zdravlje i razvoj fetusa.
Placenta predstavlja vezu između majke i fetusa, posreduje u prenosu hranljivih materija i sprečava prodiranje toksičnih elemenata. Novorođenčad zbog nerazvijenog imunskog sistema i razvoj fetalnih organa ima veće apsorpcije u odnosu na odrasle. U okviru ovog istraživanja iz tkiva placenti, ispitani su sadržaji toksičnih elemenata poput arsena, olova, aluminijuma, kadmijuma, nikla i esencijalni elementi koji su od značaja za fetus selen, cink, bakar, mangan i hrom.
Određivanje elemenata u tragovima u kliničkim uzorcima od velike su važnosti za veliki broj kliničkih ispitivanja. Propustljivost placente za esencijalne i neesencijalne elemente u tragovima su nedovoljno proučeni. Poslednjih decenija proučavana je izloženost elementima u tragovima in utero, iako je većina radova razmatrala samo nekoliko metala u tragovima ili nedovoljan broj uzoraka placente. U ovom radu smo imali za cilj da odredimo sadržaj 10 elemenata u tragovima u tkivima placenti. Dalji cilj bio je da se predloži normalni (referentni) opseg za svaki određivani element u tragovima.
Pored toga, imali smo za cilj da uporedimo naše rezultate sa drugim grupama stanovništva širom sveta, da se istakne značaj elemenata na zdravlje placente.Postupak određivanja vrednosti ovih elemenata u tragovima rađen je ICP-MS metodom.",
title = "Određivanje sadržaja toksičnih i esencijalnih elemenata u tkivu placente",
pages = "1-16",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4872"
}
Ivanović, K.. (2022). Određivanje sadržaja toksičnih i esencijalnih elemenata u tkivu placente. , 1-16.
https://hdl.handle.net/21.15107/rcub_cherry_4872
Ivanović K. Određivanje sadržaja toksičnih i esencijalnih elemenata u tkivu placente. 2022;:1-16.
https://hdl.handle.net/21.15107/rcub_cherry_4872 .
Ivanović, Katarina, "Određivanje sadržaja toksičnih i esencijalnih elemenata u tkivu placente" (2022):1-16,
https://hdl.handle.net/21.15107/rcub_cherry_4872 .

Analysis of essential, toxic, rare earth, and noble elements in maternal and umbilical cord blood

Stojsavljević, Aleksandar; Rovčanin, Marija; Miković, Željko; Perović, Milan; Jeremić, Ana; Zečević, Nebojša; Manojlović, Dragan D.

(Springer, 2022)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Marija
AU  - Miković, Željko
AU  - Perović, Milan
AU  - Jeremić, Ana
AU  - Zečević, Nebojša
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4879
AB  - Progressive industrialization in recent decades has contributed to the increase of metal levels in the environment, which has a dangerous impact on human health, primarily pregnant women. In this study, we aimed to compare levels of various elements in maternal and umbilical cord (UC) plasma samples collected from 125 healthy pregnant women, conduct correlation analysis among paired plasma samples, and compare our data with other populations worldwide. The study design included the following elements: essential (Mn, Co, Cu, Zn, Se, Mo), non-essential (Be, Al, Ni, As, Rb, Sr, Cd, Sb, Pb, U), rare earth (La, Pr, Ce, Nd, Sm, Eu, Gd, Dy, Ho, Er), and noble metals (Ru, Rh, Re, Pt). Levels of 30 elements were higher in maternal plasma than in UC plasma samples. However, no disparities at the statistically significant level were found for Be, Zn, Rb, Cd, Ce, and Ho. Correlation analysis among paired plasma samples revealed only positive/synergistic correlations of different strengths between most elements. Compared to other countries across the globe, our participants had considerably lower plasma levels of Zn and higher levels of Co, Ni, and As. This study provides not only a new and deeper comprehension, but also the first insight into the levels, correlation, distribution, and potential transplacental transfer of 30 elements.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Analysis of essential, toxic, rare earth, and noble elements in maternal and umbilical cord blood
VL  - 29
SP  - 37375
EP  - 37383
DO  - 10.1007/s11356-021-18190-y
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Marija and Miković, Željko and Perović, Milan and Jeremić, Ana and Zečević, Nebojša and Manojlović, Dragan D.",
year = "2022",
abstract = "Progressive industrialization in recent decades has contributed to the increase of metal levels in the environment, which has a dangerous impact on human health, primarily pregnant women. In this study, we aimed to compare levels of various elements in maternal and umbilical cord (UC) plasma samples collected from 125 healthy pregnant women, conduct correlation analysis among paired plasma samples, and compare our data with other populations worldwide. The study design included the following elements: essential (Mn, Co, Cu, Zn, Se, Mo), non-essential (Be, Al, Ni, As, Rb, Sr, Cd, Sb, Pb, U), rare earth (La, Pr, Ce, Nd, Sm, Eu, Gd, Dy, Ho, Er), and noble metals (Ru, Rh, Re, Pt). Levels of 30 elements were higher in maternal plasma than in UC plasma samples. However, no disparities at the statistically significant level were found for Be, Zn, Rb, Cd, Ce, and Ho. Correlation analysis among paired plasma samples revealed only positive/synergistic correlations of different strengths between most elements. Compared to other countries across the globe, our participants had considerably lower plasma levels of Zn and higher levels of Co, Ni, and As. This study provides not only a new and deeper comprehension, but also the first insight into the levels, correlation, distribution, and potential transplacental transfer of 30 elements.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Analysis of essential, toxic, rare earth, and noble elements in maternal and umbilical cord blood",
volume = "29",
pages = "37375-37383",
doi = "10.1007/s11356-021-18190-y"
}
Stojsavljević, A., Rovčanin, M., Miković, Ž., Perović, M., Jeremić, A., Zečević, N.,& Manojlović, D. D.. (2022). Analysis of essential, toxic, rare earth, and noble elements in maternal and umbilical cord blood. in Environmental Science and Pollution Research
Springer., 29, 37375-37383.
https://doi.org/10.1007/s11356-021-18190-y
Stojsavljević A, Rovčanin M, Miković Ž, Perović M, Jeremić A, Zečević N, Manojlović DD. Analysis of essential, toxic, rare earth, and noble elements in maternal and umbilical cord blood. in Environmental Science and Pollution Research. 2022;29:37375-37383.
doi:10.1007/s11356-021-18190-y .
Stojsavljević, Aleksandar, Rovčanin, Marija, Miković, Željko, Perović, Milan, Jeremić, Ana, Zečević, Nebojša, Manojlović, Dragan D., "Analysis of essential, toxic, rare earth, and noble elements in maternal and umbilical cord blood" in Environmental Science and Pollution Research, 29 (2022):37375-37383,
https://doi.org/10.1007/s11356-021-18190-y . .
4
2
2

Supplementary data for the article: Stojsavljević, A.; Rovčanin, M.; Miković, Ž.; Perović, M.; Jeremić, A.; Zečević, N.; Manojlović, D. Analysis of Essential, Toxic, Rare Earth, and Noble Elements in Maternal and Umbilical Cord Blood. Environ Sci Pollut Res 2022. https://doi.org/10.1007/s11356-021-18190-y.

Stojsavljević, Aleksandar; Rovčanin, Marija; Miković, Željko; Perović, Milan; Jeremić, Ana; Zečević, Nebojša; Manojlović, Dragan D.

(Springer, 2022)

TY  - DATA
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Marija
AU  - Miković, Željko
AU  - Perović, Milan
AU  - Jeremić, Ana
AU  - Zečević, Nebojša
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4880
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Supplementary data for the article: Stojsavljević, A.; Rovčanin, M.; Miković, Ž.; Perović, M.; Jeremić, A.; Zečević, N.; Manojlović, D. Analysis of Essential, Toxic, Rare Earth, and Noble Elements in Maternal and Umbilical Cord Blood. Environ Sci Pollut Res 2022. https://doi.org/10.1007/s11356-021-18190-y.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4880
ER  - 
@misc{
author = "Stojsavljević, Aleksandar and Rovčanin, Marija and Miković, Željko and Perović, Milan and Jeremić, Ana and Zečević, Nebojša and Manojlović, Dragan D.",
year = "2022",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Supplementary data for the article: Stojsavljević, A.; Rovčanin, M.; Miković, Ž.; Perović, M.; Jeremić, A.; Zečević, N.; Manojlović, D. Analysis of Essential, Toxic, Rare Earth, and Noble Elements in Maternal and Umbilical Cord Blood. Environ Sci Pollut Res 2022. https://doi.org/10.1007/s11356-021-18190-y.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4880"
}
Stojsavljević, A., Rovčanin, M., Miković, Ž., Perović, M., Jeremić, A., Zečević, N.,& Manojlović, D. D.. (2022). Supplementary data for the article: Stojsavljević, A.; Rovčanin, M.; Miković, Ž.; Perović, M.; Jeremić, A.; Zečević, N.; Manojlović, D. Analysis of Essential, Toxic, Rare Earth, and Noble Elements in Maternal and Umbilical Cord Blood. Environ Sci Pollut Res 2022. https://doi.org/10.1007/s11356-021-18190-y.. in Environmental Science and Pollution Research
Springer..
https://hdl.handle.net/21.15107/rcub_cherry_4880
Stojsavljević A, Rovčanin M, Miković Ž, Perović M, Jeremić A, Zečević N, Manojlović DD. Supplementary data for the article: Stojsavljević, A.; Rovčanin, M.; Miković, Ž.; Perović, M.; Jeremić, A.; Zečević, N.; Manojlović, D. Analysis of Essential, Toxic, Rare Earth, and Noble Elements in Maternal and Umbilical Cord Blood. Environ Sci Pollut Res 2022. https://doi.org/10.1007/s11356-021-18190-y.. in Environmental Science and Pollution Research. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_4880 .
Stojsavljević, Aleksandar, Rovčanin, Marija, Miković, Željko, Perović, Milan, Jeremić, Ana, Zečević, Nebojša, Manojlović, Dragan D., "Supplementary data for the article: Stojsavljević, A.; Rovčanin, M.; Miković, Ž.; Perović, M.; Jeremić, A.; Zečević, N.; Manojlović, D. Analysis of Essential, Toxic, Rare Earth, and Noble Elements in Maternal and Umbilical Cord Blood. Environ Sci Pollut Res 2022. https://doi.org/10.1007/s11356-021-18190-y." in Environmental Science and Pollution Research (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4880 .

Karakterizacija i komparacija sastava eteričnih ulja lista lovora (Laurus nobilis) sa različitih lokaliteta pri različitim tehnikama sušenja

Nikolić, Marko G.

(2022)

TY  - THES
AU  - Nikolić, Marko G.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4900
AB  - U okviru ovog rada, ispitivan je hemotip eteričnog ulja biljke lovor (Laurus nobilis) na različitim staništima, kao i kakav uticaj imaju različite tehnike sušenja na njegov sastav. Listovi lovora ubrani su na tri lokaliteta: Grbalj, Podgorica i Ulcinj. Time se pokušao pokriti čitiav opseg područja na kojima lovor uspeva u Crnoj Gori: primorski, brdsko-primorski i kontinentalni. Takođe, listovi sa sva tri lokaliteta su sušeni na sobnoj temperaturi i procesom liofilizacije. Komparacijom sastava eteričnih ulja sušenih listova sa svežim, ispitivano je koje se komponente gube i u kojoj meri. Da bi se moglo to moglo uporediti, određivana je vlaga svežih listova po Metodi sa prenosom(JUS ISO 939) koja je služila za dalji proračun. Eterična ulja iz svih uzoraka dobijana su metodom hidrodestilacije pomoću Klevendžerove aparature. Nakon ekstrakcije iz biljnog materijala, ulja su analizirana na gasnom hromatografu sa masenim detektorom, tipa kvadrupol. Komponente su identifikovane pomoću ugrađenih spektralnih baza podataka a njihova potvrda vršena je pomoću Kovačevih retencionih indeksa. Analizom dobijenih eteričnih ulja, utvrđen je hemotip biljke lovor sa ovih prostora, kao i njegove razlike u biljkama sa različitih lokaliteta. Grafički i tabelarno dati su podaci o uticaju obe metode sušenja lista biljke na sastav i kvalitet eteričnog ulja dobijenog iz lista. Iz priloženih podataka moglo se zaključiti da sušenje lista ima veliki uticaj na sastav i prinos eteričnog ulja. Metoda sušenja na sobnoj temperaturi pokazala se kao nešto blaža u odnosu na metodu liofilizacije.
T1  - Karakterizacija i komparacija sastava eteričnih ulja lista lovora (Laurus nobilis) sa različitih lokaliteta pri različitim tehnikama sušenja
SP  - 2
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4900
ER  - 
@mastersthesis{
author = "Nikolić, Marko G.",
year = "2022",
abstract = "U okviru ovog rada, ispitivan je hemotip eteričnog ulja biljke lovor (Laurus nobilis) na različitim staništima, kao i kakav uticaj imaju različite tehnike sušenja na njegov sastav. Listovi lovora ubrani su na tri lokaliteta: Grbalj, Podgorica i Ulcinj. Time se pokušao pokriti čitiav opseg područja na kojima lovor uspeva u Crnoj Gori: primorski, brdsko-primorski i kontinentalni. Takođe, listovi sa sva tri lokaliteta su sušeni na sobnoj temperaturi i procesom liofilizacije. Komparacijom sastava eteričnih ulja sušenih listova sa svežim, ispitivano je koje se komponente gube i u kojoj meri. Da bi se moglo to moglo uporediti, određivana je vlaga svežih listova po Metodi sa prenosom(JUS ISO 939) koja je služila za dalji proračun. Eterična ulja iz svih uzoraka dobijana su metodom hidrodestilacije pomoću Klevendžerove aparature. Nakon ekstrakcije iz biljnog materijala, ulja su analizirana na gasnom hromatografu sa masenim detektorom, tipa kvadrupol. Komponente su identifikovane pomoću ugrađenih spektralnih baza podataka a njihova potvrda vršena je pomoću Kovačevih retencionih indeksa. Analizom dobijenih eteričnih ulja, utvrđen je hemotip biljke lovor sa ovih prostora, kao i njegove razlike u biljkama sa različitih lokaliteta. Grafički i tabelarno dati su podaci o uticaju obe metode sušenja lista biljke na sastav i kvalitet eteričnog ulja dobijenog iz lista. Iz priloženih podataka moglo se zaključiti da sušenje lista ima veliki uticaj na sastav i prinos eteričnog ulja. Metoda sušenja na sobnoj temperaturi pokazala se kao nešto blaža u odnosu na metodu liofilizacije.",
title = "Karakterizacija i komparacija sastava eteričnih ulja lista lovora (Laurus nobilis) sa različitih lokaliteta pri različitim tehnikama sušenja",
pages = "2-54",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4900"
}
Nikolić, M. G.. (2022). Karakterizacija i komparacija sastava eteričnih ulja lista lovora (Laurus nobilis) sa različitih lokaliteta pri različitim tehnikama sušenja. , 2-54.
https://hdl.handle.net/21.15107/rcub_cherry_4900
Nikolić MG. Karakterizacija i komparacija sastava eteričnih ulja lista lovora (Laurus nobilis) sa različitih lokaliteta pri različitim tehnikama sušenja. 2022;:2-54.
https://hdl.handle.net/21.15107/rcub_cherry_4900 .
Nikolić, Marko G., "Karakterizacija i komparacija sastava eteričnih ulja lista lovora (Laurus nobilis) sa različitih lokaliteta pri različitim tehnikama sušenja" (2022):2-54,
https://hdl.handle.net/21.15107/rcub_cherry_4900 .