TY  - DATA
AU  - Đurđić, Slađana
AU  - Vlahović, Filip
AU  - Markićević, Milan
AU  - Mutić, Jelena
AU  - Manojlović, Dragan D.
AU  - Stanković, Vesna
AU  - Švorc, Ľubomír
AU  - Stanković, Dalibor
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5812
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5839
AB  - Herein, a screen–printed diamond electrode (SPDE) coupled with a “point-of-care” platform (30 µL-drop concepts, single-drop-detection approach) was successfully applied for the electrochemical determination of pterostilbene (PTS). Cyclic voltammetry identified irreversible oxidation of PTS, where oxidation peak was shown to be strongly dependent on the pH of the working environmental. Although the proposition of the detailed electrochemical oxidation mechanism of PTS goes out of the scope of the present research, we have determined the most probable reactive site of our analyte, by utilizing DFT-based reactivity descriptors (Fukui functions). For electrochemical quantification of PTS, oxidation peak at 0.32 V (vs. Ag/AgCl) was followed in presence of 0.5 mol L−1 of Briton–Robinson buffer solution (pH = 9). Coupled with the optimized parameters of differential pulse voltammetry (DPV), SPDE detected PTS in two linear ranges (first range was from 0.011 to 0.912 µmol L−1; second range was from 0.912 to 4.420 µmol L−1), providing the LOD and LOQ on a nanomolar level (3.1 nmol L−1 and 10.0 nmol L−1, respectively). The selectivity of the optimized DPV method was found to be excellent, with the current changes of less than 7%, in the presence of ten times higher concentrations of the certain interferences. The practical applicability of the SPDE and single-drop-detection approach in dietary supplements (with a declared PTS content of 50 mg/tablet), with the recovery values ranging from 95 to 102%, shows that the developed method has high potential for precise and accurate PTS detection, as well as exceptional miniaturization possibilities of relevant equipment for on-site sensing.
PB  - MDPI
T2  - Chemosensors
T1  - Supplementary material for: Đurđić, S., Vlahović, F., Markićević, M., Mutić, J., Manojlović, D., Stanković, V., Švorc, Ľ.,& Stanković, D.. (2023). Application of Screen Printed Diamond Electrode, Coupled with “Point-of-Care” Platform, for Nanomolar Quantification of Phytonutrient Pterostilbene in Dietary Supplements: An Experimental Study Supported by Theory. in Chemosensors MDPI., 11(1), 15. https://doi.org/10.3390/chemosensors11010015
VL  - 11
IS  - 1
SP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5839
ER  - 

@misc{
author = "Đurđić, Slađana and Vlahović, Filip and Markićević, Milan and Mutić, Jelena and Manojlović, Dragan D. and Stanković, Vesna and Švorc, Ľubomír and Stanković, Dalibor",
abstract = "Herein, a screen–printed diamond electrode (SPDE) coupled with a “point-of-care” platform (30 µL-drop concepts, single-drop-detection approach) was successfully applied for the electrochemical determination of pterostilbene (PTS). Cyclic voltammetry identified irreversible oxidation of PTS, where oxidation peak was shown to be strongly dependent on the pH of the working environmental. Although the proposition of the detailed electrochemical oxidation mechanism of PTS goes out of the scope of the present research, we have determined the most probable reactive site of our analyte, by utilizing DFT-based reactivity descriptors (Fukui functions). For electrochemical quantification of PTS, oxidation peak at 0.32 V (vs. Ag/AgCl) was followed in presence of 0.5 mol L−1 of Briton–Robinson buffer solution (pH = 9). Coupled with the optimized parameters of differential pulse voltammetry (DPV), SPDE detected PTS in two linear ranges (first range was from 0.011 to 0.912 µmol L−1; second range was from 0.912 to 4.420 µmol L−1), providing the LOD and LOQ on a nanomolar level (3.1 nmol L−1 and 10.0 nmol L−1, respectively). The selectivity of the optimized DPV method was found to be excellent, with the current changes of less than 7%, in the presence of ten times higher concentrations of the certain interferences. The practical applicability of the SPDE and single-drop-detection approach in dietary supplements (with a declared PTS content of 50 mg/tablet), with the recovery values ranging from 95 to 102%, shows that the developed method has high potential for precise and accurate PTS detection, as well as exceptional miniaturization possibilities of relevant equipment for on-site sensing.",
publisher = "MDPI",
journal = "Chemosensors",
title = "Supplementary material for: Đurđić, S., Vlahović, F., Markićević, M., Mutić, J., Manojlović, D., Stanković, V., Švorc, Ľ.,& Stanković, D.. (2023). Application of Screen Printed Diamond Electrode, Coupled with “Point-of-Care” Platform, for Nanomolar Quantification of Phytonutrient Pterostilbene in Dietary Supplements: An Experimental Study Supported by Theory. in Chemosensors MDPI., 11(1), 15. https://doi.org/10.3390/chemosensors11010015",
volume = "11",
number = "1",
pages = "15",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5839"
}

Đurđić, S., Vlahović, F., Markićević, M., Mutić, J., Manojlović, D. D., Stanković, V., Švorc, Ľ.,& Stanković, D..Supplementary material for: Đurđić, S., Vlahović, F., Markićević, M., Mutić, J., Manojlović, D., Stanković, V., Švorc, Ľ.,& Stanković, D.. (2023). Application of Screen Printed Diamond Electrode, Coupled with “Point-of-Care” Platform, for Nanomolar Quantification of Phytonutrient Pterostilbene in Dietary Supplements: An Experimental Study Supported by Theory. in Chemosensors MDPI., 11(1), 15. https://doi.org/10.3390/chemosensors11010015. in Chemosensors
MDPI., 11(1), 15.
https://hdl.handle.net/21.15107/rcub_cherry_5839

Đurđić S, Vlahović F, Markićević M, Mutić J, Manojlović DD, Stanković V, Švorc Ľ, Stanković D. Supplementary material for: Đurđić, S., Vlahović, F., Markićević, M., Mutić, J., Manojlović, D., Stanković, V., Švorc, Ľ.,& Stanković, D.. (2023). Application of Screen Printed Diamond Electrode, Coupled with “Point-of-Care” Platform, for Nanomolar Quantification of Phytonutrient Pterostilbene in Dietary Supplements: An Experimental Study Supported by Theory. in Chemosensors MDPI., 11(1), 15. https://doi.org/10.3390/chemosensors11010015. in Chemosensors.11(1):15.
https://hdl.handle.net/21.15107/rcub_cherry_5839 .

Đurđić, Slađana, Vlahović, Filip, Markićević, Milan, Mutić, Jelena, Manojlović, Dragan D., Stanković, Vesna, Švorc, Ľubomír, Stanković, Dalibor, "Supplementary material for: Đurđić, S., Vlahović, F., Markićević, M., Mutić, J., Manojlović, D., Stanković, V., Švorc, Ľ.,& Stanković, D.. (2023). Application of Screen Printed Diamond Electrode, Coupled with “Point-of-Care” Platform, for Nanomolar Quantification of Phytonutrient Pterostilbene in Dietary Supplements: An Experimental Study Supported by Theory. in Chemosensors MDPI., 11(1), 15. https://doi.org/10.3390/chemosensors11010015" in Chemosensors, 11, no. 1:15,
https://hdl.handle.net/21.15107/rcub_cherry_5839 .

TY  - DATA
AU  - Aleksić, Jovana
AU  - Stojanović, Milovan
AU  - Bošković, Jakša
AU  - Baranac-Stojanović, Marija
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5846
AB  - We present the green, highly atom-economical, solid-state silica gel-catalyzed synthesis of polysubstituted 1,4- and 1,2-dihydropyridines (DHPs) from commercially available materials, amines and ethyl propiolate. The DHP skeleton was assembled by heating the reactants and silica gel in a closed vessel. Aliphatic amines provided 1,4-isomers as the main or only DHP products, but the reactions of aromatic amines yielded a mixture of 1,4- and 1,2-isomers. To the best of our knowledge, this is the first example of the formation of a 1,2-DHP structure by the reaction of an amine with propiolic ester. Addition of 1 mass percent of H2SO4 to silica gel shifted the product distribution to 1,4-DHP as the main or the only isomer obtained. Experimental and theoretical analyses led to the identification of two key intermediates en route to DHPs and the explanation of the observed regioisomeric ratios. 1,2-DHPs show blue-cyan fluorescence in MeOH with the quantum yield Φ = 0.10–0.22 relative to quinine sulfate Φ = 0.58 and 1,4-DHPs show blue-violet fluorescence with Φ = 0.09–0.81.
PB  - Royal Society of Chemistry
T2  - Organic & Biomolecular Chemistry
T1  - Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F
VL  - 21
IS  - 6
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5846
ER  - 

@misc{
author = "Aleksić, Jovana and Stojanović, Milovan and Bošković, Jakša and Baranac-Stojanović, Marija",
abstract = "We present the green, highly atom-economical, solid-state silica gel-catalyzed synthesis of polysubstituted 1,4- and 1,2-dihydropyridines (DHPs) from commercially available materials, amines and ethyl propiolate. The DHP skeleton was assembled by heating the reactants and silica gel in a closed vessel. Aliphatic amines provided 1,4-isomers as the main or only DHP products, but the reactions of aromatic amines yielded a mixture of 1,4- and 1,2-isomers. To the best of our knowledge, this is the first example of the formation of a 1,2-DHP structure by the reaction of an amine with propiolic ester. Addition of 1 mass percent of H2SO4 to silica gel shifted the product distribution to 1,4-DHP as the main or the only isomer obtained. Experimental and theoretical analyses led to the identification of two key intermediates en route to DHPs and the explanation of the observed regioisomeric ratios. 1,2-DHPs show blue-cyan fluorescence in MeOH with the quantum yield Φ = 0.10–0.22 relative to quinine sulfate Φ = 0.58 and 1,4-DHPs show blue-violet fluorescence with Φ = 0.09–0.81.",
publisher = "Royal Society of Chemistry",
journal = "Organic & Biomolecular Chemistry",
title = "Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F",
volume = "21",
number = "6",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5846"
}

Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M..Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6).
https://hdl.handle.net/21.15107/rcub_cherry_5846

Aleksić J, Stojanović M, Bošković J, Baranac-Stojanović M. Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F. in Organic & Biomolecular Chemistry.21(6).
https://hdl.handle.net/21.15107/rcub_cherry_5846 .

Aleksić, Jovana, Stojanović, Milovan, Bošković, Jakša, Baranac-Stojanović, Marija, "Supplementary material for: Aleksić, J., Stojanović, M., Bošković, J.,& Baranac-Stojanović, M.. (2023). Solid-state silica gel-catalyzed synthesis of fluorescent polysubstituted 1,4- and 1,2-dihydropyridines. in Organic & Biomolecular Chemistry
Royal Society of Chemistry., 21(6), 1187-1205.
https://doi.org/10.1039/D2OB02119F" in Organic & Biomolecular Chemistry, 21, no. 6,
https://hdl.handle.net/21.15107/rcub_cherry_5846 .

TY  - DATA
AU  - Penjišević, Jelena 
AU  - Šukalović, Vladimir 
AU  - Dukić-Stefanović, Slađana
AU  - Deuther-Conrad, Winnie
AU  - Andrić, Deana 
AU  - Kostić-Rajačić, Slađana
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5845
AB  - Serotonin receptors modulate numerous behavioral and neuropsychological processes. Therefore, they are the target for the action of many drugs, such as antipsychotics, antidepressants, antiemetics, migraine remedies, and many others. The 5-HT1A receptors have been involved in the pathogenesis and treatment of anxiety and depression and represent a promising target for new drugs with reduced extrapyramidal side effects. In most antidepressants, a piperazine-based structural motif can be identified as a common moiety. Here we describe the synthesis, pharmacological, and in silico characterization of a novel arylpiperazines series with excellent 5-HT1A affinity. The final compounds, 4a, 8a, and 8b, were selected according to predictions of in silico pharmacokinetics, docking analysis, and molecular dynamics in conjunction with physical properties, and metabolic stability. The accentuated molecules could serve as a lead compound for developing 5-HT1A drug-like molecules for depression treatment.
PB  - Elsevier
T2  - Arabian Journal of Chemistry
T1  - Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636
VL  - 16
IS  - 4
SP  - 104636
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5845
ER  - 

@misc{
author = "Penjišević, Jelena  and Šukalović, Vladimir  and Dukić-Stefanović, Slađana and Deuther-Conrad, Winnie and Andrić, Deana  and Kostić-Rajačić, Slađana",
abstract = "Serotonin receptors modulate numerous behavioral and neuropsychological processes. Therefore, they are the target for the action of many drugs, such as antipsychotics, antidepressants, antiemetics, migraine remedies, and many others. The 5-HT1A receptors have been involved in the pathogenesis and treatment of anxiety and depression and represent a promising target for new drugs with reduced extrapyramidal side effects. In most antidepressants, a piperazine-based structural motif can be identified as a common moiety. Here we describe the synthesis, pharmacological, and in silico characterization of a novel arylpiperazines series with excellent 5-HT1A affinity. The final compounds, 4a, 8a, and 8b, were selected according to predictions of in silico pharmacokinetics, docking analysis, and molecular dynamics in conjunction with physical properties, and metabolic stability. The accentuated molecules could serve as a lead compound for developing 5-HT1A drug-like molecules for depression treatment.",
publisher = "Elsevier",
journal = "Arabian Journal of Chemistry",
title = "Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636",
volume = "16",
number = "4",
pages = "104636",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5845"
}

Penjišević, J., Šukalović, V., Dukić-Stefanović, S., Deuther-Conrad, W., Andrić, D.,& Kostić-Rajačić, S..Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636. in Arabian Journal of Chemistry
Elsevier., 16(4), 104636.
https://hdl.handle.net/21.15107/rcub_cherry_5845

Penjišević J, Šukalović V, Dukić-Stefanović S, Deuther-Conrad W, Andrić D, Kostić-Rajačić S. Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636. in Arabian Journal of Chemistry.16(4):104636.
https://hdl.handle.net/21.15107/rcub_cherry_5845 .

Penjišević, Jelena , Šukalović, Vladimir , Dukić-Stefanović, Slađana, Deuther-Conrad, Winnie, Andrić, Deana , Kostić-Rajačić, Slađana, "Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636" in Arabian Journal of Chemistry, 16, no. 4:104636,
https://hdl.handle.net/21.15107/rcub_cherry_5845 .

TY  - JOUR
AU  - Spahić, Darko
AU  - Šajnović, Aleksandra
AU  - Burazer, Nikola
AU  - Radisavljević, Marija
AU  - Jovančićević, Branimir
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6260
AB  - With the objective to quantify the Neogene extensional geodynamic drivers of the Peri Pannonian Realm s.l., a series of 1D basin models were carried out by integrating the available geological and geochemical data extracted from the landlocked Toplica Basin (central-southern Serbia; Serbo-Macedonian Unit). The modeling was conducted on four wells located immediately south of Jastrebac Mt., in the area of the Toplica Basin. The investigated southernmost point of the Peri Pannonian Ream s.l. (areas surrounding the basin, to the south of rivers Danube and Sava) underwent intense tectonically-driven subsidence episodes, which lasted from the Lower Miocene up to the Pliocene. The restored Neogene burial history (subsidence rates), paleotemperature, and calibrated paleo-heat fluxes (or changes in the ancient crustal or basal heat flow) indicated a two-staged crustal stretching of the wider Toplica area. The combined tectonic, i.e., thermal subsidence, resulted in a very high sedimentation rate, having an average ca. 300–400 m/Ma (Čučale unit). The intense tectonically-driven subsidence is consistent with the involvement of extensional processes, affecting the crustal and lithospheric layers of the retreating Adria/Dinarides, amplified by a distant Carpathian rollback stage. The regional extension led to the asymmetric opening and the formation of the Toplica Lower Miocene mesosaline to the freshwater lacustrine system. Higher participation of mixed microbiologically reworked/terrestrial organic matter incorporated in oil-prone Type II kerogen mainly characterized the investigated lacustrine sedimentary sequence. The latest Oligocene–earliest Neogene subsidence onset was characterized by elevated temperatures, affecting the early development of the oil window (ca. at 16 Ma). The 1D models also showed that the Lower Čučale unit achieved ca. 50% transformation ratio for the Type II organic matter. The two intra-Neogene extensional episodes suggested the existence of the Early Neogene “syn-rift” stage, followed by a thermal sagging. The Neogene continuous thinning of the overriding Serbo-Macedonian continental crust contributed to the “negative inversion” of the former pre-Neogene nappes. Therefore, Neogene tectonic reactivation induced contemporaneous subsidence, further allowing the extensional rise of the Jastrebac core complex.
PB  - Elsevier
T2  - Geoenergy Science and Engineering
T1  - Neogene subsidence rates of the southern Peri Pannonian realm (1D basin modeling): Constraints on the extensional geodynamic drivers of the asymmetric Toplica basin (central-southern Serbia)
VL  - 226
SP  - 211714
DO  - 10.1016/j.geoen.2023.211714
ER  - 

@article{
author = "Spahić, Darko and Šajnović, Aleksandra and Burazer, Nikola and Radisavljević, Marija and Jovančićević, Branimir",
year = "2023",
abstract = "With the objective to quantify the Neogene extensional geodynamic drivers of the Peri Pannonian Realm s.l., a series of 1D basin models were carried out by integrating the available geological and geochemical data extracted from the landlocked Toplica Basin (central-southern Serbia; Serbo-Macedonian Unit). The modeling was conducted on four wells located immediately south of Jastrebac Mt., in the area of the Toplica Basin. The investigated southernmost point of the Peri Pannonian Ream s.l. (areas surrounding the basin, to the south of rivers Danube and Sava) underwent intense tectonically-driven subsidence episodes, which lasted from the Lower Miocene up to the Pliocene. The restored Neogene burial history (subsidence rates), paleotemperature, and calibrated paleo-heat fluxes (or changes in the ancient crustal or basal heat flow) indicated a two-staged crustal stretching of the wider Toplica area. The combined tectonic, i.e., thermal subsidence, resulted in a very high sedimentation rate, having an average ca. 300–400 m/Ma (Čučale unit). The intense tectonically-driven subsidence is consistent with the involvement of extensional processes, affecting the crustal and lithospheric layers of the retreating Adria/Dinarides, amplified by a distant Carpathian rollback stage. The regional extension led to the asymmetric opening and the formation of the Toplica Lower Miocene mesosaline to the freshwater lacustrine system. Higher participation of mixed microbiologically reworked/terrestrial organic matter incorporated in oil-prone Type II kerogen mainly characterized the investigated lacustrine sedimentary sequence. The latest Oligocene–earliest Neogene subsidence onset was characterized by elevated temperatures, affecting the early development of the oil window (ca. at 16 Ma). The 1D models also showed that the Lower Čučale unit achieved ca. 50% transformation ratio for the Type II organic matter. The two intra-Neogene extensional episodes suggested the existence of the Early Neogene “syn-rift” stage, followed by a thermal sagging. The Neogene continuous thinning of the overriding Serbo-Macedonian continental crust contributed to the “negative inversion” of the former pre-Neogene nappes. Therefore, Neogene tectonic reactivation induced contemporaneous subsidence, further allowing the extensional rise of the Jastrebac core complex.",
publisher = "Elsevier",
journal = "Geoenergy Science and Engineering",
title = "Neogene subsidence rates of the southern Peri Pannonian realm (1D basin modeling): Constraints on the extensional geodynamic drivers of the asymmetric Toplica basin (central-southern Serbia)",
volume = "226",
pages = "211714",
doi = "10.1016/j.geoen.2023.211714"
}

Spahić, D., Šajnović, A., Burazer, N., Radisavljević, M.,& Jovančićević, B.. (2023). Neogene subsidence rates of the southern Peri Pannonian realm (1D basin modeling): Constraints on the extensional geodynamic drivers of the asymmetric Toplica basin (central-southern Serbia). in Geoenergy Science and Engineering
Elsevier., 226, 211714.
https://doi.org/10.1016/j.geoen.2023.211714

Spahić D, Šajnović A, Burazer N, Radisavljević M, Jovančićević B. Neogene subsidence rates of the southern Peri Pannonian realm (1D basin modeling): Constraints on the extensional geodynamic drivers of the asymmetric Toplica basin (central-southern Serbia). in Geoenergy Science and Engineering. 2023;226:211714.
doi:10.1016/j.geoen.2023.211714 .

Spahić, Darko, Šajnović, Aleksandra, Burazer, Nikola, Radisavljević, Marija, Jovančićević, Branimir, "Neogene subsidence rates of the southern Peri Pannonian realm (1D basin modeling): Constraints on the extensional geodynamic drivers of the asymmetric Toplica basin (central-southern Serbia)" in Geoenergy Science and Engineering, 226 (2023):211714,
https://doi.org/10.1016/j.geoen.2023.211714 . .

TY  - JOUR
AU  - Đurović, Živanka
AU  - Mutavdžin, Slavica
AU  - Drobnjaković, Miloš
AU  - Đurović, Marko
AU  - Cvrkota, Irena
AU  - Jovanović, Vladimir
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6256
AB  - AbstractGlioblastoma (GBM) is the most common primary malignant brain tumor in adults. Homocysteine (Hcy) has a detrimental influence on human neurons, considering that human GBM cells undergo cell death already at D,L-Hcy concentrations in culture medium of 50 μM. This data demonstrate that Hcy is a potent gliotoxic agent capable of inducing the death of human glial cells already at concentrations reached in brain during hyperhomocysteinemia. The one retrospective study found that the serum vitamin B12 level can be used to predict survival time in metastatic cancer patients including neurological cancer. Cancer risk increases with elevated vitamin B12 level, mostly within the first year of the follow-up period, suggesting that vitamin B12 level could be used as a cancer diagnostic marker. In addition, the relationship between elevated vitamin B12 level and poor cancer survival time has been reported. Previous investigation suggests that the folate supplementation could be used as an adjuvant in antiglioma therapy to limit the low DNA methylation level because this confers a poor prognosis in glioblastoma multiforme patients. Taking into account all presented data, it can be concluded that effect of homocystein, folic acid and vitamin B12 on formation, development and outcome of treatment in patients with
PB  - Sciendo
T2  - Experimental and Applied Biomedical Research (EABR)
T1  - Relationship Between Malignant Brain Tumors and Values of Homocysteine, Folic Acid and Vitamin B12
VL  - 24
IS  - 1
SP  - 69
EP  - 74
DO  - 10.2478/sjecr-2018-0045
ER  - 

@article{
author = "Đurović, Živanka and Mutavdžin, Slavica and Drobnjaković, Miloš and Đurović, Marko and Cvrkota, Irena and Jovanović, Vladimir",
year = "2023",
abstract = "AbstractGlioblastoma (GBM) is the most common primary malignant brain tumor in adults. Homocysteine (Hcy) has a detrimental influence on human neurons, considering that human GBM cells undergo cell death already at D,L-Hcy concentrations in culture medium of 50 μM. This data demonstrate that Hcy is a potent gliotoxic agent capable of inducing the death of human glial cells already at concentrations reached in brain during hyperhomocysteinemia. The one retrospective study found that the serum vitamin B12 level can be used to predict survival time in metastatic cancer patients including neurological cancer. Cancer risk increases with elevated vitamin B12 level, mostly within the first year of the follow-up period, suggesting that vitamin B12 level could be used as a cancer diagnostic marker. In addition, the relationship between elevated vitamin B12 level and poor cancer survival time has been reported. Previous investigation suggests that the folate supplementation could be used as an adjuvant in antiglioma therapy to limit the low DNA methylation level because this confers a poor prognosis in glioblastoma multiforme patients. Taking into account all presented data, it can be concluded that effect of homocystein, folic acid and vitamin B12 on formation, development and outcome of treatment in patients with",
publisher = "Sciendo",
journal = "Experimental and Applied Biomedical Research (EABR)",
title = "Relationship Between Malignant Brain Tumors and Values of Homocysteine, Folic Acid and Vitamin B12",
volume = "24",
number = "1",
pages = "69-74",
doi = "10.2478/sjecr-2018-0045"
}

Đurović, Ž., Mutavdžin, S., Drobnjaković, M., Đurović, M., Cvrkota, I.,& Jovanović, V.. (2023). Relationship Between Malignant Brain Tumors and Values of Homocysteine, Folic Acid and Vitamin B12. in Experimental and Applied Biomedical Research (EABR)
Sciendo., 24(1), 69-74.
https://doi.org/10.2478/sjecr-2018-0045

Đurović Ž, Mutavdžin S, Drobnjaković M, Đurović M, Cvrkota I, Jovanović V. Relationship Between Malignant Brain Tumors and Values of Homocysteine, Folic Acid and Vitamin B12. in Experimental and Applied Biomedical Research (EABR). 2023;24(1):69-74.
doi:10.2478/sjecr-2018-0045 .

Đurović, Živanka, Mutavdžin, Slavica, Drobnjaković, Miloš, Đurović, Marko, Cvrkota, Irena, Jovanović, Vladimir, "Relationship Between Malignant Brain Tumors and Values of Homocysteine, Folic Acid and Vitamin B12" in Experimental and Applied Biomedical Research (EABR), 24, no. 1 (2023):69-74,
https://doi.org/10.2478/sjecr-2018-0045 . .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Ilić-Tomić, Tatjana
AU  - Đorđević, Iris
AU  - Anđelković, Boban
AU  - Tešević, Slobodan
AU  - Milosavljević, Slobodan
AU  - Asakawa, Yoshinori
PY  - 2023
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6259
AB  - Bisbibenzyls are specialized metabolites found exclusively in liverworts, until recently; they represent chemical markers of liverworts. Their occurrence in vascular plants was noticed in 2007, when they were found in Primula veris subsp. macrocalyx from Russia. This report prompted us to chemically analyze the two most common Serbian Primula species, P. veris subsp. columnae and P. acaulis, in order to determine the presence of bisbibenzyls in them. Our study revealed nine structurally distinct bisbibenzyls (1–9), identified based on 1D and 2D NMR, IR, UV and HRESIMS data. Among them were five previously undescribed compounds (2–6). The remaining com­ pounds found and previously described in the literature were: the bisbibenzyls riccardin C (1), isoperrottetin A (7), isoplagiochin E (8) and 11-O-demethylmarchantin I (9), as well as 4-hydroxyphenylmethylketone (10) and 4-hydroxy-3-methoxyphenylmethylketone (11). Riccardin C was the most dominant bisbibenzyl in both species studied. Previously, it was the first bisbibenzyl found in vascular plants (P. veris subsp. macrocalyx). An assessment of the cytotoxic activity of the isolated compounds against A549 lung cancer and healthy MRC5 cell lines was also the subject of our study. Compounds 6 and 9 exhibited significant cytotoxic activity expressed by IC50 values of 12 μM, but the selectivity was not satisfactory.
PB  - Elsevier
T2  - Phytochemistry
T1  - Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L
VL  - 212
EP  - 113719
DO  - 10.1016/j.phytochem.2023.113719
ER  - 

@article{
author = "Novaković, Miroslav and Ilić-Tomić, Tatjana and Đorđević, Iris and Anđelković, Boban and Tešević, Slobodan and Milosavljević, Slobodan and Asakawa, Yoshinori",
year = "2023, 2023",
abstract = "Bisbibenzyls are specialized metabolites found exclusively in liverworts, until recently; they represent chemical markers of liverworts. Their occurrence in vascular plants was noticed in 2007, when they were found in Primula veris subsp. macrocalyx from Russia. This report prompted us to chemically analyze the two most common Serbian Primula species, P. veris subsp. columnae and P. acaulis, in order to determine the presence of bisbibenzyls in them. Our study revealed nine structurally distinct bisbibenzyls (1–9), identified based on 1D and 2D NMR, IR, UV and HRESIMS data. Among them were five previously undescribed compounds (2–6). The remaining com­ pounds found and previously described in the literature were: the bisbibenzyls riccardin C (1), isoperrottetin A (7), isoplagiochin E (8) and 11-O-demethylmarchantin I (9), as well as 4-hydroxyphenylmethylketone (10) and 4-hydroxy-3-methoxyphenylmethylketone (11). Riccardin C was the most dominant bisbibenzyl in both species studied. Previously, it was the first bisbibenzyl found in vascular plants (P. veris subsp. macrocalyx). An assessment of the cytotoxic activity of the isolated compounds against A549 lung cancer and healthy MRC5 cell lines was also the subject of our study. Compounds 6 and 9 exhibited significant cytotoxic activity expressed by IC50 values of 12 μM, but the selectivity was not satisfactory.",
publisher = "Elsevier",
journal = "Phytochemistry",
title = "Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L",
volume = "212",
pages = "113719",
doi = "10.1016/j.phytochem.2023.113719"
}

Novaković, M., Ilić-Tomić, T., Đorđević, I., Anđelković, B., Tešević, S., Milosavljević, S.,& Asakawa, Y.. (2023). Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L. in Phytochemistry
Elsevier., 212.
https://doi.org/10.1016/j.phytochem.2023.113719

Novaković M, Ilić-Tomić T, Đorđević I, Anđelković B, Tešević S, Milosavljević S, Asakawa Y. Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L. in Phytochemistry. 2023;212:null-113719.
doi:10.1016/j.phytochem.2023.113719 .

Novaković, Miroslav, Ilić-Tomić, Tatjana, Đorđević, Iris, Anđelković, Boban, Tešević, Slobodan, Milosavljević, Slobodan, Asakawa, Yoshinori, "Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L" in Phytochemistry, 212 (2023),
https://doi.org/10.1016/j.phytochem.2023.113719 . .

TY  - JOUR
AU  - Stojković, Pavle
AU  - Kostić, Ana
AU  - Lupšić, Ema
AU  - Jovanović, Nataša Terzić
AU  - Novaković, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6258
AB  - The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and 11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 µM. The concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a, 12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress accompanied by inhibition of mitochondria.
PB  - Elsevier
T2  - Bioorganic Chemistry
T1  - Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells
VL  - 138
SP  - 106605
DO  - 10.1016/j.bioorg.2023.106605
ER  - 

@article{
author = "Stojković, Pavle and Kostić, Ana and Lupšić, Ema and Jovanović, Nataša Terzić and Novaković, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Pešić, Milica and Opsenica, Igor M.",
year = "2023",
abstract = "The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and 11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 µM. The concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a, 12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress accompanied by inhibition of mitochondria.",
publisher = "Elsevier",
journal = "Bioorganic Chemistry",
title = "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells",
volume = "138",
pages = "106605",
doi = "10.1016/j.bioorg.2023.106605"
}

Stojković, P., Kostić, A., Lupšić, E., Jovanović, N. T., Novaković, M., Nedialkov, P., Trendafilova, A., Pešić, M.,& Opsenica, I. M.. (2023). Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry
Elsevier., 138, 106605.
https://doi.org/10.1016/j.bioorg.2023.106605

Stojković P, Kostić A, Lupšić E, Jovanović NT, Novaković M, Nedialkov P, Trendafilova A, Pešić M, Opsenica IM. Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry. 2023;138:106605.
doi:10.1016/j.bioorg.2023.106605 .

Stojković, Pavle, Kostić, Ana, Lupšić, Ema, Jovanović, Nataša Terzić, Novaković, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Pešić, Milica, Opsenica, Igor M., "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells" in Bioorganic Chemistry, 138 (2023):106605,
https://doi.org/10.1016/j.bioorg.2023.106605 . .

TY  - JOUR
AU  - Zelenović, Nevena D.
AU  - Kojadinović, Milica I.
AU  - Filipović, Lidija
AU  - Vučić, Vesna M.
AU  - Milčić, Miloš K.
AU  - Arsic, Aleksndra
AU  - Popović, Milica M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6257
AB  - Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.
PB  - SAGE Publications
T2  - Natural Product Communications
T1  - Interactions of Different Urolithins With Bovine Serum Albumin
VL  - 18
IS  - 5
DO  - 10.1177/1934578X231169366
ER  - 

@article{
author = "Zelenović, Nevena D. and Kojadinović, Milica I. and Filipović, Lidija and Vučić, Vesna M. and Milčić, Miloš K. and Arsic, Aleksndra and Popović, Milica M.",
year = "2023",
abstract = "Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.",
publisher = "SAGE Publications",
journal = "Natural Product Communications",
title = "Interactions of Different Urolithins With Bovine Serum Albumin",
volume = "18",
number = "5",
doi = "10.1177/1934578X231169366"
}

Zelenović, N. D., Kojadinović, M. I., Filipović, L., Vučić, V. M., Milčić, M. K., Arsic, A.,& Popović, M. M.. (2023). Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications
SAGE Publications., 18(5).
https://doi.org/10.1177/1934578X231169366

Zelenović ND, Kojadinović MI, Filipović L, Vučić VM, Milčić MK, Arsic A, Popović MM. Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications. 2023;18(5).
doi:10.1177/1934578X231169366 .

Zelenović, Nevena D., Kojadinović, Milica I., Filipović, Lidija, Vučić, Vesna M., Milčić, Miloš K., Arsic, Aleksndra, Popović, Milica M., "Interactions of Different Urolithins With Bovine Serum Albumin" in Natural Product Communications, 18, no. 5 (2023),
https://doi.org/10.1177/1934578X231169366 . .

TY  - JOUR
AU  - Andjelković, Ljubica
AU  - Šuljagić, Marija
AU  - Mirković, Miljana
AU  - Pavlović, Vera P.
AU  - Petronijević, Ivan
AU  - Stanković, Dalibor
AU  - Jeremić, Dejan
AU  - Uskoković, Vuk
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6254
AB  - The combination of an intense absorption of visible light and p-type semiconducting nature makes spinel cobalt oxide (Co3O4) a very attractive material for various optoelectronic applications. However, the traditional methods for its synthesis have been either time- and energy-consuming or relying on toxic chemicals. To solve this issue, a simple, facile, and eco-friendly method of synthesis was successfully developed to obtain spinel Co3O4 nanoparticles. The novel method for obtaining pure and monophasic Co3O4 reported here is based on the thermal decomposition of hexaaquacobalt(II) D-camphor10-sulfonate at 900 °C. This fast solid-state synthesis route overcomes the disadvantages of many combustion methods, most critically by avoiding the use of toxic organic solvents. The synthesized material was subjected to a detailed characterization to assess its potential for use as a nanocatalyst. The band gap measurements indicated the presence of two band gaps, one at 2.10 eV and another at 1.22 eV, confirming the purity and semiconducting properties of the sample. The electrochemical studies demonstrated a significant enhancement in the electron transfer kinetics with the addition of the synthesized Co3O4 to the carbon-paste electrode, leading to an enhanced electrocatalytic performance. These prominent functional properties, suitable for a wide range of technological applications, pave way for the implementation of the reported method for the synthesis of Co3O4 on a larger industrial scale.
PB  - Elsevier
T2  - Ceramics International
T1  - Semiconducting cobalt oxide nanocatalyst obtained through an eco-friendly thermal decomposition
VL  - 49
IS  - 14, Part A
SP  - 23491
EP  - 23498
DO  - 10.1016/j.ceramint.2023.04.182
ER  - 

@article{
author = "Andjelković, Ljubica and Šuljagić, Marija and Mirković, Miljana and Pavlović, Vera P. and Petronijević, Ivan and Stanković, Dalibor and Jeremić, Dejan and Uskoković, Vuk",
year = "2023",
abstract = "The combination of an intense absorption of visible light and p-type semiconducting nature makes spinel cobalt oxide (Co3O4) a very attractive material for various optoelectronic applications. However, the traditional methods for its synthesis have been either time- and energy-consuming or relying on toxic chemicals. To solve this issue, a simple, facile, and eco-friendly method of synthesis was successfully developed to obtain spinel Co3O4 nanoparticles. The novel method for obtaining pure and monophasic Co3O4 reported here is based on the thermal decomposition of hexaaquacobalt(II) D-camphor10-sulfonate at 900 °C. This fast solid-state synthesis route overcomes the disadvantages of many combustion methods, most critically by avoiding the use of toxic organic solvents. The synthesized material was subjected to a detailed characterization to assess its potential for use as a nanocatalyst. The band gap measurements indicated the presence of two band gaps, one at 2.10 eV and another at 1.22 eV, confirming the purity and semiconducting properties of the sample. The electrochemical studies demonstrated a significant enhancement in the electron transfer kinetics with the addition of the synthesized Co3O4 to the carbon-paste electrode, leading to an enhanced electrocatalytic performance. These prominent functional properties, suitable for a wide range of technological applications, pave way for the implementation of the reported method for the synthesis of Co3O4 on a larger industrial scale.",
publisher = "Elsevier",
journal = "Ceramics International",
title = "Semiconducting cobalt oxide nanocatalyst obtained through an eco-friendly thermal decomposition",
volume = "49",
number = "14, Part A",
pages = "23491-23498",
doi = "10.1016/j.ceramint.2023.04.182"
}

Andjelković, L., Šuljagić, M., Mirković, M., Pavlović, V. P., Petronijević, I., Stanković, D., Jeremić, D.,& Uskoković, V.. (2023). Semiconducting cobalt oxide nanocatalyst obtained through an eco-friendly thermal decomposition. in Ceramics International
Elsevier., 49(14, Part A), 23491-23498.
https://doi.org/10.1016/j.ceramint.2023.04.182

Andjelković L, Šuljagić M, Mirković M, Pavlović VP, Petronijević I, Stanković D, Jeremić D, Uskoković V. Semiconducting cobalt oxide nanocatalyst obtained through an eco-friendly thermal decomposition. in Ceramics International. 2023;49(14, Part A):23491-23498.
doi:10.1016/j.ceramint.2023.04.182 .

Andjelković, Ljubica, Šuljagić, Marija, Mirković, Miljana, Pavlović, Vera P., Petronijević, Ivan, Stanković, Dalibor, Jeremić, Dejan, Uskoković, Vuk, "Semiconducting cobalt oxide nanocatalyst obtained through an eco-friendly thermal decomposition" in Ceramics International, 49, no. 14, Part A (2023):23491-23498,
https://doi.org/10.1016/j.ceramint.2023.04.182 . .

TY  - JOUR
AU  - Rosenthal, R. G.
AU  - Diana Zhang, X.
AU  - Ilić Đurđić, Karla
AU  - Collins, J. J.
AU  - Weitz, D. A.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6255
AB  - Enzymes are highly specific catalysts delivering improved drugs and greener industrial processes. Naturally occurring enzymes must typically be optimized which is often accomplished through directed evolution; however, this is still a labor- and capital-intensive process, due in part to multiple molecular biology steps including DNA extraction, in vitro library generation, transformation, and limited screening throughput. We present an effective and broadly applicable continuous evolution platform that enables controlled exploration of fitness landscape to evolve enzymes at ultrahigh throughput based on direct measurement of enzymatic activity. This drop-based microfluidics platform cycles cells between growth and mutagenesis followed by screening with minimal human intervention, relying on the nCas9 chimera with mutagenesis polymerase to produce in vivo gene diversification using sgRNAs tiled along the gene. We evolve alditol oxidase to change its substrate specificity towards glycerol, turning a waste product into a valuable feedstock. We identify a variant with a 10.5-fold catalytic efficiency.
PB  - John Wiley and Sons Inc
T2  - Angewandte Chemie International Edition
T1  - Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics
VL  - 62
IS  - 24
SP  - e202303112
DO  - 10.1002/anie.202303112
ER  - 

@article{
author = "Rosenthal, R. G. and Diana Zhang, X. and Ilić Đurđić, Karla and Collins, J. J. and Weitz, D. A.",
year = "2023",
abstract = "Enzymes are highly specific catalysts delivering improved drugs and greener industrial processes. Naturally occurring enzymes must typically be optimized which is often accomplished through directed evolution; however, this is still a labor- and capital-intensive process, due in part to multiple molecular biology steps including DNA extraction, in vitro library generation, transformation, and limited screening throughput. We present an effective and broadly applicable continuous evolution platform that enables controlled exploration of fitness landscape to evolve enzymes at ultrahigh throughput based on direct measurement of enzymatic activity. This drop-based microfluidics platform cycles cells between growth and mutagenesis followed by screening with minimal human intervention, relying on the nCas9 chimera with mutagenesis polymerase to produce in vivo gene diversification using sgRNAs tiled along the gene. We evolve alditol oxidase to change its substrate specificity towards glycerol, turning a waste product into a valuable feedstock. We identify a variant with a 10.5-fold catalytic efficiency.",
publisher = "John Wiley and Sons Inc",
journal = "Angewandte Chemie International Edition",
title = "Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics",
volume = "62",
number = "24",
pages = "e202303112",
doi = "10.1002/anie.202303112"
}

Rosenthal, R. G., Diana Zhang, X., Ilić Đurđić, K., Collins, J. J.,& Weitz, D. A.. (2023). Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics. in Angewandte Chemie International Edition
John Wiley and Sons Inc., 62(24), e202303112.
https://doi.org/10.1002/anie.202303112

Rosenthal RG, Diana Zhang X, Ilić Đurđić K, Collins JJ, Weitz DA. Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics. in Angewandte Chemie International Edition. 2023;62(24):e202303112.
doi:10.1002/anie.202303112 .

Rosenthal, R. G., Diana Zhang, X., Ilić Đurđić, Karla, Collins, J. J., Weitz, D. A., "Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics" in Angewandte Chemie International Edition, 62, no. 24 (2023):e202303112,
https://doi.org/10.1002/anie.202303112 . .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Kojić, Milan
AU  - Margetić, Aleksandra
AU  - Ristović, Marina
AU  - Pavlović, Marija
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6008
AB  - The combination ofb-oligosaccharides from enzymatically hydrolysed barleyb-glucan has attracted inter-est recently due to its positive effects on human health. This study aimed to assess the impact of theendo-b-1,3-1,4-glucanase enzyme fromBacillus  subtilis168 on improving the nutritional and bioactiveproperties of barleyb-glucan. A new procedure for the isolation ofb-glucan was developed, at a lowertemperature (45°C), enabling purity from starch contamination, without affecting the yield (6 gb-glucanfrom 100 g of barley flour). The endo-b-1,3-1,4-glucanase is cloned intoE. colipQE_Ek enables the highproduction and purification (82% yield, 1.8 mg mL 1and 440 U mg 1) of an enzyme identical to thenatural one (25.5 kDa). The enzymatic reaction showed high efficiency ofb-glucan degradation by recom-binant enzyme, giving a mixture of products (of which 3-O-b-cellobiosyl-D-glucose and 3-O-b-cellotriosyl-D-glucose are the most abundant), the reduction of viscosity (17%) and increase in antioxidant capacitiesby 15.2%, 30.9% and 44.0% assessed by ABTS, DPPH and ORAC, respectively. These results indicatethe possible application of endo-b-1,3-1,4-glucanase enzyme in improving the properties of barleyb-glucan used as functional foods.
PB  - Wiley
T2  - International Journal of Food Science and Technology
T1  - Improvement of nutritional and bioactive properties of barleyb-glucan-based food products usingBacillus subtilis168endo-b-1,3-1,4-glucanase
VL  - 58
IS  - 12
SP  - 6825
EP  - 6835
DO  - 10.1111/ijfs.16647
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Kojić, Milan and Margetić, Aleksandra and Ristović, Marina and Pavlović, Marija and Nikolić, Stefan and Vujčić, Zoran",
year = "2023",
abstract = "The combination ofb-oligosaccharides from enzymatically hydrolysed barleyb-glucan has attracted inter-est recently due to its positive effects on human health. This study aimed to assess the impact of theendo-b-1,3-1,4-glucanase enzyme fromBacillus  subtilis168 on improving the nutritional and bioactiveproperties of barleyb-glucan. A new procedure for the isolation ofb-glucan was developed, at a lowertemperature (45°C), enabling purity from starch contamination, without affecting the yield (6 gb-glucanfrom 100 g of barley flour). The endo-b-1,3-1,4-glucanase is cloned intoE. colipQE_Ek enables the highproduction and purification (82% yield, 1.8 mg mL 1and 440 U mg 1) of an enzyme identical to thenatural one (25.5 kDa). The enzymatic reaction showed high efficiency ofb-glucan degradation by recom-binant enzyme, giving a mixture of products (of which 3-O-b-cellobiosyl-D-glucose and 3-O-b-cellotriosyl-D-glucose are the most abundant), the reduction of viscosity (17%) and increase in antioxidant capacitiesby 15.2%, 30.9% and 44.0% assessed by ABTS, DPPH and ORAC, respectively. These results indicatethe possible application of endo-b-1,3-1,4-glucanase enzyme in improving the properties of barleyb-glucan used as functional foods.",
publisher = "Wiley",
journal = "International Journal of Food Science and Technology",
title = "Improvement of nutritional and bioactive properties of barleyb-glucan-based food products usingBacillus subtilis168endo-b-1,3-1,4-glucanase",
volume = "58",
number = "12",
pages = "6825-6835",
doi = "10.1111/ijfs.16647"
}

Šokarda Slavić, M., Kojić, M., Margetić, A., Ristović, M., Pavlović, M., Nikolić, S.,& Vujčić, Z.. (2023). Improvement of nutritional and bioactive properties of barleyb-glucan-based food products usingBacillus subtilis168endo-b-1,3-1,4-glucanase. in International Journal of Food Science and Technology
Wiley., 58(12), 6825-6835.
https://doi.org/10.1111/ijfs.16647

Šokarda Slavić M, Kojić M, Margetić A, Ristović M, Pavlović M, Nikolić S, Vujčić Z. Improvement of nutritional and bioactive properties of barleyb-glucan-based food products usingBacillus subtilis168endo-b-1,3-1,4-glucanase. in International Journal of Food Science and Technology. 2023;58(12):6825-6835.
doi:10.1111/ijfs.16647 .

Šokarda Slavić, Marinela, Kojić, Milan, Margetić, Aleksandra, Ristović, Marina, Pavlović, Marija, Nikolić, Stefan, Vujčić, Zoran, "Improvement of nutritional and bioactive properties of barleyb-glucan-based food products usingBacillus subtilis168endo-b-1,3-1,4-glucanase" in International Journal of Food Science and Technology, 58, no. 12 (2023):6825-6835,
https://doi.org/10.1111/ijfs.16647 . .

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić-Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/5633
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Authenticity assessment of cultivated berries via phenolic profiles of seeds
VL  - 402
SP  - 134184
DO  - 10.1016/j.foodchem.2022.134184
ER  - 

@article{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić-Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Authenticity assessment of cultivated berries via phenolic profiles of seeds",
volume = "402",
pages = "134184",
doi = "10.1016/j.foodchem.2022.134184"
}

Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić-Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D.,& Trifković, J.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry
Elsevier., 402, 134184.
https://doi.org/10.1016/j.foodchem.2022.134184

Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić-Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica D, Trifković J. Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry. 2023;402:134184.
doi:10.1016/j.foodchem.2022.134184 .

Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić-Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka, Trifković, Jelena, "Authenticity assessment of cultivated berries via phenolic profiles of seeds" in Food Chemistry, 402 (2023):134184,
https://doi.org/10.1016/j.foodchem.2022.134184 . .

TY  - DATA
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić-Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5633
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184
VL  - 402
SP  - 134184
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5633
ER  - 

@misc{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić-Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184",
volume = "402",
pages = "134184",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5633"
}

Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić-Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D.,& Trifković, J.. (2023). Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry
Elsevier., 402, 134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633

Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić-Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica D, Trifković J. Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry. 2023;402:134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633 .

Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić-Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka, Trifković, Jelena, "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184" in Food Chemistry, 402 (2023):134184,
https://hdl.handle.net/21.15107/rcub_cherry_5633 .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Prđun, Saša
AU  - Bubalo, Dragan
AU  - Lušić, Dražen
AU  - Vahčić, Nada
AU  - Kopjar, Nevenka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5874
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study
VL  - 24
IS  - 3
SP  - 1903
DO  - 10.3390/ijms24031903
ER  - 

@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka",
year = "2023",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study",
volume = "24",
number = "3",
pages = "1903",
doi = "10.3390/ijms24031903"
}

Jurič, A., Brčić Karačonji, I., Gašić, U. M., Milojković-Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences
MDPI., 24(3), 1903.
https://doi.org/10.3390/ijms24031903

Jurič A, Brčić Karačonji I, Gašić UM, Milojković-Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3):1903.
doi:10.3390/ijms24031903 .

Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023):1903,
https://doi.org/10.3390/ijms24031903 . .

TY  - DATA
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Prđun, Saša
AU  - Bubalo, Dragan
AU  - Lušić, Dražen
AU  - Vahčić, Nada
AU  - Kopjar, Nevenka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5875
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study
VL  - 24
IS  - 3
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5875
ER  - 

@misc{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka",
year = "2023",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study",
volume = "24",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5875"
}

Jurič, A., Brčić Karačonji, I., Gašić, U. M., Milojković-Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences
MDPI., 24(3).
https://hdl.handle.net/21.15107/rcub_cherry_5875

Jurič A, Brčić Karačonji I, Gašić UM, Milojković-Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3).
https://hdl.handle.net/21.15107/rcub_cherry_5875 .

Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5875 .

TY  - JOUR
AU  - Mosić, Mirjana D.
AU  - Trifković, Jelena
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5876
AB  - The aim of this study is to develop a rapid, effect-directed screening method for quality assessment of bee pollen-honey mixtures. The comparative antioxidant potential and phenolic content of honey, bee pollen, and the bee pollen-honey mixtures, was performed using spectrophotometry. The total phenolic content and antioxidative activity of bee pollen-honey mixtures with 20 % bee pollen share were in the range 3.03–3.11 mg GAE/g, and 6.02–6.96 mmol TE/kg, respectively, while mixtures with 30 % bee pollen share contained 3.92–4.18 mg GAE/g, and 9.69–10.11 mmol TE/kg. Chromatographic fingerprint of bee pollen-honey mixtures was performed by high-performance thin-layer chromatography with conditions developed by authors and reported for the first time. Fingerprint analysis hyphenated with chemometrics enabled authenticity assessments of honey in mixtures. Results indicate that bee pollen-honey mixtures represent a food with highly, both, nutritious characteristics and health-promoting effect.
PB  - Wiley
T2  - Chemistry & Biodiversity
T2  - Chemistry & Biodiversity
T1  - Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics
VL  - 20
IS  - 3
SP  - e202201141
DO  - 10.1002/cbdv.202201141
ER  - 

@article{
author = "Mosić, Mirjana D. and Trifković, Jelena and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "The aim of this study is to develop a rapid, effect-directed screening method for quality assessment of bee pollen-honey mixtures. The comparative antioxidant potential and phenolic content of honey, bee pollen, and the bee pollen-honey mixtures, was performed using spectrophotometry. The total phenolic content and antioxidative activity of bee pollen-honey mixtures with 20 % bee pollen share were in the range 3.03–3.11 mg GAE/g, and 6.02–6.96 mmol TE/kg, respectively, while mixtures with 30 % bee pollen share contained 3.92–4.18 mg GAE/g, and 9.69–10.11 mmol TE/kg. Chromatographic fingerprint of bee pollen-honey mixtures was performed by high-performance thin-layer chromatography with conditions developed by authors and reported for the first time. Fingerprint analysis hyphenated with chemometrics enabled authenticity assessments of honey in mixtures. Results indicate that bee pollen-honey mixtures represent a food with highly, both, nutritious characteristics and health-promoting effect.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity, Chemistry & Biodiversity",
title = "Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics",
volume = "20",
number = "3",
pages = "e202201141",
doi = "10.1002/cbdv.202201141"
}

Mosić, M. D., Trifković, J., Ristivojević, P.,& Milojković-Opsenica, D.. (2023). Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics. in Chemistry & Biodiversity
Wiley., 20(3), e202201141.
https://doi.org/10.1002/cbdv.202201141

Mosić MD, Trifković J, Ristivojević P, Milojković-Opsenica D. Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics. in Chemistry & Biodiversity. 2023;20(3):e202201141.
doi:10.1002/cbdv.202201141 .

Mosić, Mirjana D., Trifković, Jelena, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics" in Chemistry & Biodiversity, 20, no. 3 (2023):e202201141,
https://doi.org/10.1002/cbdv.202201141 . .

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Nešić, Jelena
AU  - Andrić, Filip
AU  - Nedić, Nebojša
AU  - Stanisavljević, Ljubiša
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5895
AB  - Propolis is a resinous natural substance collected by honeybees from different plant sources. The element content of propolis is influenced by the content of elements in the soil, climatic factors, and the degree of pollution. The aim of the study was to characterize element composition and content of toxic elements in 51 propolis collected from different locations in Serbia and to evaluate the differences among them. Determination of the 21 elements were performed using Inductively coupled plasma - optical emission spectrometry. K was the most abundant element, followed by Ca, Mg, Si, Fe, Zn, and Al. The microelements B, Ba, Mn, Na, Pb, and Ti were also present in propolis, while Co, Cr, Cu, Mo, Ni, Sr, and V were found in trace. Toxic elements such as Pb, As, and Cd were found in the trace. Kruskal-Wallis tests differentiate groups of samples by geographical origin. The presence of B, Fe, Sr, Ti, Zn, and As demonstrated statistical significance between six regions of Serbia.
PB  - Wiley
T2  - Chemistry & Biodiversity
T2  - Chemistry & Biodiversity
T1  - Elemental Profile of Propolis from Different Areas of Serbia
VL  - 20
IS  - 3
SP  - e202201140
DO  - 10.1002/cbdv.202201140
ER  - 

@article{
author = "Ristivojević, Petar and Nešić, Jelena and Andrić, Filip and Nedić, Nebojša and Stanisavljević, Ljubiša and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Propolis is a resinous natural substance collected by honeybees from different plant sources. The element content of propolis is influenced by the content of elements in the soil, climatic factors, and the degree of pollution. The aim of the study was to characterize element composition and content of toxic elements in 51 propolis collected from different locations in Serbia and to evaluate the differences among them. Determination of the 21 elements were performed using Inductively coupled plasma - optical emission spectrometry. K was the most abundant element, followed by Ca, Mg, Si, Fe, Zn, and Al. The microelements B, Ba, Mn, Na, Pb, and Ti were also present in propolis, while Co, Cr, Cu, Mo, Ni, Sr, and V were found in trace. Toxic elements such as Pb, As, and Cd were found in the trace. Kruskal-Wallis tests differentiate groups of samples by geographical origin. The presence of B, Fe, Sr, Ti, Zn, and As demonstrated statistical significance between six regions of Serbia.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity, Chemistry & Biodiversity",
title = "Elemental Profile of Propolis from Different Areas of Serbia",
volume = "20",
number = "3",
pages = "e202201140",
doi = "10.1002/cbdv.202201140"
}

Ristivojević, P., Nešić, J., Andrić, F., Nedić, N., Stanisavljević, L., Milojković-Opsenica, D.,& Trifković, J.. (2023). Elemental Profile of Propolis from Different Areas of Serbia. in Chemistry & Biodiversity
Wiley., 20(3), e202201140.
https://doi.org/10.1002/cbdv.202201140

Ristivojević P, Nešić J, Andrić F, Nedić N, Stanisavljević L, Milojković-Opsenica D, Trifković J. Elemental Profile of Propolis from Different Areas of Serbia. in Chemistry & Biodiversity. 2023;20(3):e202201140.
doi:10.1002/cbdv.202201140 .

Ristivojević, Petar, Nešić, Jelena, Andrić, Filip, Nedić, Nebojša, Stanisavljević, Ljubiša, Milojković-Opsenica, Dušanka, Trifković, Jelena, "Elemental Profile of Propolis from Different Areas of Serbia" in Chemistry & Biodiversity, 20, no. 3 (2023):e202201140,
https://doi.org/10.1002/cbdv.202201140 . .

TY  - CONF
AU  - Jakanovski, Mihajlo V.
AU  - Dramićanin, Aleksandra M.
AU  - Horvacki, Nikola
AU  - Trifković, Jelena
AU  - Rašić, Dubravka
AU  - Brčić Karačonji, Irena
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5933
AB  - Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products
SP  - 73
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5933
ER  - 

@conference{
author = "Jakanovski, Mihajlo V. and Dramićanin, Aleksandra M. and Horvacki, Nikola and Trifković, Jelena and Rašić, Dubravka and Brčić Karačonji, Irena and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products",
pages = "73-74",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5933"
}

Jakanovski, M. V., Dramićanin, A. M., Horvacki, N., Trifković, J., Rašić, D., Brčić Karačonji, I.,& Milojković-Opsenica, D.. (2023). Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo., 73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933

Jakanovski MV, Dramićanin AM, Horvacki N, Trifković J, Rašić D, Brčić Karačonji I, Milojković-Opsenica D. Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933 .

Jakanovski, Mihajlo V., Dramićanin, Aleksandra M., Horvacki, Nikola, Trifković, Jelena, Rašić, Dubravka, Brčić Karačonji, Irena, Milojković-Opsenica, Dušanka, "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):73-74,
https://hdl.handle.net/21.15107/rcub_cherry_5933 .

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Drami, Aleksandra
AU  - Mosić, Mirjana
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5974
AB  - Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).

Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.

Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.

References:
[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).
[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process
SP  - 175
EP  - 175
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5974
ER  - 

@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Drami, Aleksandra and Mosić, Mirjana and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).

Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.

Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.

References:
[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).
[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process",
pages = "175-175",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5974"
}

Momirović, N., Nikolić, D., Jakanovski, M. V., Drami, A., Mosić, M., Momirović, N.,& Milojković-Opsenica, D.. (2023). The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo., 175-175.
https://hdl.handle.net/21.15107/rcub_cherry_5974

Momirović N, Nikolić D, Jakanovski MV, Drami A, Mosić M, Momirović N, Milojković-Opsenica D. The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:175-175.
https://hdl.handle.net/21.15107/rcub_cherry_5974 .

Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Drami, Aleksandra, Mosić, Mirjana, Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):175-175,
https://hdl.handle.net/21.15107/rcub_cherry_5974 .

TY  - CONF
AU  - Cvijanović, Vojin
AU  - Dramićanin, Aleksandra
AU  - Sarić, Beka
AU  - Jakanovski, Mihajlo V.
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5976
AB  - Interest in production of protected crops has grown significantly over the past decade. Different cultivation systems (conventional, integral, and organic) affect the biological activity, which is ultimately reflected in the high quality of the fruits of different vegetable crops. In accordance with the requirements for health-safe products without residues of harmful substances in fruits, there is an increasing need for the production of tomatoes in integral and organic production systems. One of the most important features of these vegetables is their high quality and health safety, high nutritional and biological value. Agricultural production systems and growing practices are critical factors in determining the nutritional quality of tomato fruits [1]. In tomato, the sugar content is one of the important factors and one of the most significant parameters from the aspect of food quality [1]. Therefore, the aim of this work is to monitor fluctuations in sugars content, as parameters
that determine the nutritional value in tomato cultivars, induced by growing under integral and organic conditions. For this purpose, a set of sixteen samples of four types of tomatoes - beef, grapolo, mini and midi plum, and cherry - was analyzed. Each type of tomato included two varieties, grown in two agricultural systems - integral and organic. The sugars profile was obtained using High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAECPAD). The content of eleven sugar components was determined. Fructose and glucose were the major sugar compounds [2], while the sugar microcomponents were trehalose, arabinose, melibiose, sucrose, isomaltose, gentiobiose, raffinose, maltose, and panose. The results showed differences between samples produced in integral and organic growing systems, primarily in microsugar components. Higher content of trehalose and melibiose was found in samples obtained from organic production.

Fig.1. Principal Component Analysis (A)- The difference between integral (I) and organic (O) type of production; score plot (1a) – tomato samples: integral samples (I) 9-14, organic samples (O) 1-8, and loading plot (1b), and results of the Mann-Whitney U test (B) - sugar markers of type of production.

Acknowledgments: The authors would like to thank the Zeleni hit d.o.o. from Belgrade, Serbia, for field trials and support. This work has been supported by the Ministry of Science, Technological 
Development and Innovation of Republic of Serbia, Contract number: 451-03- 47/2023-01/200168, 451-03-47/2023-01/200288 and 451-03-47/2023-01/ 200045.

References:
[1] E. Rosa-Martínez, M. D. García-Martínez, A. M. Adalid-Martínez, L. Pereira-Dias, C. Casanova, E. Soler, M. Rosario Figas, M. Dolores
Raigon, M. Plazas, S. Soler, J. Prohens, Food Research International, 147 (2021) 110531.
[2] C. Agius, S. von Tucher, B. Poppenberger, W. Rozhon, MethodsX, 5 (2018). 537-550.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023
T1  - The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars
SP  - 87
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5976
ER  - 

@conference{
author = "Cvijanović, Vojin and Dramićanin, Aleksandra and Sarić, Beka and Jakanovski, Mihajlo V. and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Interest in production of protected crops has grown significantly over the past decade. Different cultivation systems (conventional, integral, and organic) affect the biological activity, which is ultimately reflected in the high quality of the fruits of different vegetable crops. In accordance with the requirements for health-safe products without residues of harmful substances in fruits, there is an increasing need for the production of tomatoes in integral and organic production systems. One of the most important features of these vegetables is their high quality and health safety, high nutritional and biological value. Agricultural production systems and growing practices are critical factors in determining the nutritional quality of tomato fruits [1]. In tomato, the sugar content is one of the important factors and one of the most significant parameters from the aspect of food quality [1]. Therefore, the aim of this work is to monitor fluctuations in sugars content, as parameters
that determine the nutritional value in tomato cultivars, induced by growing under integral and organic conditions. For this purpose, a set of sixteen samples of four types of tomatoes - beef, grapolo, mini and midi plum, and cherry - was analyzed. Each type of tomato included two varieties, grown in two agricultural systems - integral and organic. The sugars profile was obtained using High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAECPAD). The content of eleven sugar components was determined. Fructose and glucose were the major sugar compounds [2], while the sugar microcomponents were trehalose, arabinose, melibiose, sucrose, isomaltose, gentiobiose, raffinose, maltose, and panose. The results showed differences between samples produced in integral and organic growing systems, primarily in microsugar components. Higher content of trehalose and melibiose was found in samples obtained from organic production.

Fig.1. Principal Component Analysis (A)- The difference between integral (I) and organic (O) type of production; score plot (1a) – tomato samples: integral samples (I) 9-14, organic samples (O) 1-8, and loading plot (1b), and results of the Mann-Whitney U test (B) - sugar markers of type of production.

Acknowledgments: The authors would like to thank the Zeleni hit d.o.o. from Belgrade, Serbia, for field trials and support. This work has been supported by the Ministry of Science, Technological 
Development and Innovation of Republic of Serbia, Contract number: 451-03- 47/2023-01/200168, 451-03-47/2023-01/200288 and 451-03-47/2023-01/ 200045.

References:
[1] E. Rosa-Martínez, M. D. García-Martínez, A. M. Adalid-Martínez, L. Pereira-Dias, C. Casanova, E. Soler, M. Rosario Figas, M. Dolores
Raigon, M. Plazas, S. Soler, J. Prohens, Food Research International, 147 (2021) 110531.
[2] C. Agius, S. von Tucher, B. Poppenberger, W. Rozhon, MethodsX, 5 (2018). 537-550.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023",
title = "The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars",
pages = "87-87",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5976"
}

Cvijanović, V., Dramićanin, A., Sarić, B., Jakanovski, M. V., Momirović, N.,& Milojković-Opsenica, D.. (2023). The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023
Beograd : Srpsko hemijsko društvo., 87-87.
https://hdl.handle.net/21.15107/rcub_cherry_5976

Cvijanović V, Dramićanin A, Sarić B, Jakanovski MV, Momirović N, Milojković-Opsenica D. The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023. 2023;:87-87.
https://hdl.handle.net/21.15107/rcub_cherry_5976 .

Cvijanović, Vojin, Dramićanin, Aleksandra, Sarić, Beka, Jakanovski, Mihajlo V., Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023 (2023):87-87,
https://hdl.handle.net/21.15107/rcub_cherry_5976 .

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Drami, Aleksandra
AU  - Mosić, Mirjana
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5975
AB  - Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.References:[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5975
ER  - 

@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Drami, Aleksandra and Mosić, Mirjana and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.References:[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5975"
}

Momirović, N., Nikolić, D., Jakanovski, M. V., Drami, A., Mosić, M., Momirović, N.,& Milojković-Opsenica, D.. (2023). The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo..
https://hdl.handle.net/21.15107/rcub_cherry_5975

Momirović N, Nikolić D, Jakanovski MV, Drami A, Mosić M, Momirović N, Milojković-Opsenica D. The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_5975 .

Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Drami, Aleksandra, Mosić, Mirjana, Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5975 .

TY  - JOUR
AU  - Jankov, Milica
AU  - Ristivojević, Petar
AU  - Cvijetić, Ilija
AU  - Milojković Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6232
AB  - High-Performance Thin-Layer Chromatography (HPTLC)-radical scavenging capacity (RSC) assays are stan- dard techniques for the separation and identification of antioxidants from complex mixtures. HPTLC coupled with DPPH · visualization of chromatograms allows for the detection of individual antioxidants. However, other HPTLC-RSC assays that recognize compounds exhibiting different mechanisms of radical- scavenging activity are rarely reported. In this study, we developed an integrated approach that combines five HPTLC-RSC assays, principal component analysis (PCA) and quantum chemical calculations to assess the antioxidant capacity of Sempervivum tectorum L. leaf extracts. Two HPTLC assays - potassium hexa- cyanoferrate(III) total reducing power assay (TRP) and total antioxidant capacity by phosphomolybdenum method (TAC) - were developed for the first time. The method supports a more in-depth study of the RSC of natural products, as it compares the radical scavenging fingerprints of S. tectorum leaf extracts and recognizes differences in their individual bioactive constituents. Kaempferol, kaempferol 3- O -glucoside, quercetin 3- O -glucoside, caffeic acid, and gallic acid were identified as the compounds that discriminate HPTLC-RSC assays according to their mechanism of action and capture the similarities between 20 S. tec- torum samples. Additionally, DFT calculations on M06–2X/6–31 + G (d,p) level were applied to map ther- modynamic feasibility of hydrogen atom transfer (HAT) and single electron transfer (SET) mechanisms of the identified compounds. Based on experimental and theoretical results, a combination of HPTLC-ABTS and HPTLC-TAC assays were proposed as the optimal method for mapping the antioxidants from S. tec- torum . This study represents a step forward in identifying and quantifying individual antioxidants from complex food and natural product matrices in a more rational manner.
PB  - Elsevier
T2  - Journal of Chromatography A
T1  - Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach
VL  - 1703
SP  - 464082
DO  - 10.1016/j.chroma.2023.464082
ER  - 

@article{
author = "Jankov, Milica and Ristivojević, Petar and Cvijetić, Ilija and Milojković Opsenica, Dušanka",
year = "2023",
abstract = "High-Performance Thin-Layer Chromatography (HPTLC)-radical scavenging capacity (RSC) assays are stan- dard techniques for the separation and identification of antioxidants from complex mixtures. HPTLC coupled with DPPH · visualization of chromatograms allows for the detection of individual antioxidants. However, other HPTLC-RSC assays that recognize compounds exhibiting different mechanisms of radical- scavenging activity are rarely reported. In this study, we developed an integrated approach that combines five HPTLC-RSC assays, principal component analysis (PCA) and quantum chemical calculations to assess the antioxidant capacity of Sempervivum tectorum L. leaf extracts. Two HPTLC assays - potassium hexa- cyanoferrate(III) total reducing power assay (TRP) and total antioxidant capacity by phosphomolybdenum method (TAC) - were developed for the first time. The method supports a more in-depth study of the RSC of natural products, as it compares the radical scavenging fingerprints of S. tectorum leaf extracts and recognizes differences in their individual bioactive constituents. Kaempferol, kaempferol 3- O -glucoside, quercetin 3- O -glucoside, caffeic acid, and gallic acid were identified as the compounds that discriminate HPTLC-RSC assays according to their mechanism of action and capture the similarities between 20 S. tec- torum samples. Additionally, DFT calculations on M06–2X/6–31 + G (d,p) level were applied to map ther- modynamic feasibility of hydrogen atom transfer (HAT) and single electron transfer (SET) mechanisms of the identified compounds. Based on experimental and theoretical results, a combination of HPTLC-ABTS and HPTLC-TAC assays were proposed as the optimal method for mapping the antioxidants from S. tec- torum . This study represents a step forward in identifying and quantifying individual antioxidants from complex food and natural product matrices in a more rational manner.",
publisher = "Elsevier",
journal = "Journal of Chromatography A",
title = "Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach",
volume = "1703",
pages = "464082",
doi = "10.1016/j.chroma.2023.464082"
}

Jankov, M., Ristivojević, P., Cvijetić, I.,& Milojković Opsenica, D.. (2023). Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach. in Journal of Chromatography A
Elsevier., 1703, 464082.
https://doi.org/10.1016/j.chroma.2023.464082

Jankov M, Ristivojević P, Cvijetić I, Milojković Opsenica D. Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach. in Journal of Chromatography A. 2023;1703:464082.
doi:10.1016/j.chroma.2023.464082 .

Jankov, Milica, Ristivojević, Petar, Cvijetić, Ilija, Milojković Opsenica, Dušanka, "Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach" in Journal of Chromatography A, 1703 (2023):464082,
https://doi.org/10.1016/j.chroma.2023.464082 . .

TY  - CONF
AU  - Jankov, Milica
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6209
AB  - Sempervivum tectorum L. is a perennial plant with succulent leaves arranged in a rosette. Despite its wide use in traditional medicine for the treatment of various changes on the skin, nervousness, anxiety, menstrual ailments, etc., it still does not have a biography in the European Pharmacopoeia¹. The antibacterial effect of juice squeezed from the leaves and tea made from dried leaves was tested against bacteria that cause inflammation of the ear and bladder². Direct bioautography on HPTLC plates for the investigation of antibacterial activity of the houseleek leaves extracts was done for the first time. Four HPTLC bioassays were developed for gram-positive bacteria (B. subtilis, M. lysodeikticus, MRSA, and S. aureus), and two for gramnegative bacteria (E. coli and K. pneumoniae). Antibacterial activity of grampositive bacteria comes from active compounds at RF 0.57 and 0.92. HPTLC bioassays show higher sensitivity for gram-negative bacteria, as numerous active bands were observed at RF 0.03, 0.38, 0.42, 0.56, 0.57, 0.86 and 0.92. By applying chemometrics and principal components analysis, the HPTLC bioassays of grampositive bacteria were separated into two groups on the scores plot graphic along PC1 axis. The bioassays for B. subtilis and M. lysodeikticus form a less uniform group in the upper left quadrant, while the bioassays for MRSA and S. aureus form a homogeneous group in the lower right quadrant. Presence or absence of the active bands at RF 0.57 and 0.92 affect these separations. The bioassays for the two gramnegative bacteria are separated along both axes on the scores plot graphic from each other but also from the other bioassays for the gram-positive bacteria. This separation is influenced by the presence of numerous active bands. For further investigation it is necessary to identify compounds responsible for the antibacterial activity of the houseleek leaves extracts.
C3  - 9th Conference of Young Chemists of Serbia, Book of abstracts
T1  - Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6209
ER  - 

@conference{
author = "Jankov, Milica and Milojković-Opsenica, Dušanka and Ristivojević, Petar",
year = "2023",
abstract = "Sempervivum tectorum L. is a perennial plant with succulent leaves arranged in a rosette. Despite its wide use in traditional medicine for the treatment of various changes on the skin, nervousness, anxiety, menstrual ailments, etc., it still does not have a biography in the European Pharmacopoeia¹. The antibacterial effect of juice squeezed from the leaves and tea made from dried leaves was tested against bacteria that cause inflammation of the ear and bladder². Direct bioautography on HPTLC plates for the investigation of antibacterial activity of the houseleek leaves extracts was done for the first time. Four HPTLC bioassays were developed for gram-positive bacteria (B. subtilis, M. lysodeikticus, MRSA, and S. aureus), and two for gramnegative bacteria (E. coli and K. pneumoniae). Antibacterial activity of grampositive bacteria comes from active compounds at RF 0.57 and 0.92. HPTLC bioassays show higher sensitivity for gram-negative bacteria, as numerous active bands were observed at RF 0.03, 0.38, 0.42, 0.56, 0.57, 0.86 and 0.92. By applying chemometrics and principal components analysis, the HPTLC bioassays of grampositive bacteria were separated into two groups on the scores plot graphic along PC1 axis. The bioassays for B. subtilis and M. lysodeikticus form a less uniform group in the upper left quadrant, while the bioassays for MRSA and S. aureus form a homogeneous group in the lower right quadrant. Presence or absence of the active bands at RF 0.57 and 0.92 affect these separations. The bioassays for the two gramnegative bacteria are separated along both axes on the scores plot graphic from each other but also from the other bioassays for the gram-positive bacteria. This separation is influenced by the presence of numerous active bands. For further investigation it is necessary to identify compounds responsible for the antibacterial activity of the houseleek leaves extracts.",
journal = "9th Conference of Young Chemists of Serbia, Book of abstracts",
title = "Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6209"
}

Jankov, M., Milojković-Opsenica, D.,& Ristivojević, P.. (2023). Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics. in 9th Conference of Young Chemists of Serbia, Book of abstracts.
https://hdl.handle.net/21.15107/rcub_cherry_6209

Jankov M, Milojković-Opsenica D, Ristivojević P. Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics. in 9th Conference of Young Chemists of Serbia, Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6209 .

Jankov, Milica, Milojković-Opsenica, Dušanka, Ristivojević, Petar, "Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics" in 9th Conference of Young Chemists of Serbia, Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6209 .

TY  - JOUR
AU  - Lazović, Mila
AU  - Cvijetić, Ilija
AU  - Jankov, Milica
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
AU  - Ristivojević, Petar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6230
AB  - Natural Eutectic Solvents (NES) are a promising class of environmentally friendly liquids that offer an alternative to conventional organic solvents. To investigate their potential for extracting phenolic compounds from plant materials, we employed the COSMO-RS (COnductor like Screening MOdel for Real Solvents) computational method. Nineteen NES solvents were prepared to determine the most selective and effective solvent for extracting phenolic compounds from Teucrium chamaedrys using both theoretical and experimental evaluation. Screening of extraction efficiency was performed by quantification of individual compounds using ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS), as well as spectrophotometric assays (total phenolic content, total flavonoid content, and radical scavenging activity). In this paper, we propose a new approach to model NES properties using COSMO-RS. While the majority of previous studies have modeled these solvents as individual or pseudo-component complexes between hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA), we have developed a ternary HBD:HBA:water supramolecular complex model to represent NES solution structures more accurately. This model provides insights into the intermolecular interactions driving the extraction process and predicts extraction efficiencies that agree with experimental data. Two eutectic mixtures (choline chloride:succinic acid in molar ratio 1:1 and 20% of water (w/w) and choline chloride: glycerol in molar ratio 1:1 and 20% of water (w/w)) showed strong affinity towards phenolic compounds. Overall, our findings suggest that the ternary complex model is a more appropriate approach for modeling NES properties.
PB  - Elsevier
T2  - Journal of Molecular Liquids
T1  - COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys
VL  - 387
SP  - 122649
DO  - 10.1016/j.molliq.2023.122649
ER  - 

@article{
author = "Lazović, Mila and Cvijetić, Ilija and Jankov, Milica and Milojković-Opsenica, Dušanka and Trifković, Jelena and Ristivojević, Petar",
year = "2023",
abstract = "Natural Eutectic Solvents (NES) are a promising class of environmentally friendly liquids that offer an alternative to conventional organic solvents. To investigate their potential for extracting phenolic compounds from plant materials, we employed the COSMO-RS (COnductor like Screening MOdel for Real Solvents) computational method. Nineteen NES solvents were prepared to determine the most selective and effective solvent for extracting phenolic compounds from Teucrium chamaedrys using both theoretical and experimental evaluation. Screening of extraction efficiency was performed by quantification of individual compounds using ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS), as well as spectrophotometric assays (total phenolic content, total flavonoid content, and radical scavenging activity). In this paper, we propose a new approach to model NES properties using COSMO-RS. While the majority of previous studies have modeled these solvents as individual or pseudo-component complexes between hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA), we have developed a ternary HBD:HBA:water supramolecular complex model to represent NES solution structures more accurately. This model provides insights into the intermolecular interactions driving the extraction process and predicts extraction efficiencies that agree with experimental data. Two eutectic mixtures (choline chloride:succinic acid in molar ratio 1:1 and 20% of water (w/w) and choline chloride: glycerol in molar ratio 1:1 and 20% of water (w/w)) showed strong affinity towards phenolic compounds. Overall, our findings suggest that the ternary complex model is a more appropriate approach for modeling NES properties.",
publisher = "Elsevier",
journal = "Journal of Molecular Liquids",
title = "COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys",
volume = "387",
pages = "122649",
doi = "10.1016/j.molliq.2023.122649"
}

Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2023). COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys. in Journal of Molecular Liquids
Elsevier., 387, 122649.
https://doi.org/10.1016/j.molliq.2023.122649

Lazović M, Cvijetić I, Jankov M, Milojković-Opsenica D, Trifković J, Ristivojević P. COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys. in Journal of Molecular Liquids. 2023;387:122649.
doi:10.1016/j.molliq.2023.122649 .

Lazović, Mila, Cvijetić, Ilija, Jankov, Milica, Milojković-Opsenica, Dušanka, Trifković, Jelena, Ristivojević, Petar, "COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys" in Journal of Molecular Liquids, 387 (2023):122649,
https://doi.org/10.1016/j.molliq.2023.122649 . .

TY  - CONF
AU  - Lazović, Mila Č.
AU  - Jović, Marko D.
AU  - Dimkić, Ivica Z.
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
AU  - Trifković, Jelena Đ.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6220
AB  - Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.
C3  - XXII Congress EuroFoodChem, Book of abstracts
T1  - Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6220
ER  - 

@conference{
author = "Lazović, Mila Č. and Jović, Marko D. and Dimkić, Ivica Z. and Milojković-Opsenica, Dušanka and Ristivojević, Petar and Trifković, Jelena Đ.",
year = "2023",
abstract = "Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.",
journal = "XXII Congress EuroFoodChem, Book of abstracts",
title = "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6220"
}

Lazović, M. Č., Jović, M. D., Dimkić, I. Z., Milojković-Opsenica, D., Ristivojević, P.,& Trifković, J. Đ.. (2023). Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts.
https://hdl.handle.net/21.15107/rcub_cherry_6220

Lazović MČ, Jović MD, Dimkić IZ, Milojković-Opsenica D, Ristivojević P, Trifković JĐ. Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6220 .

Lazović, Mila Č., Jović, Marko D., Dimkić, Ivica Z., Milojković-Opsenica, Dušanka, Ristivojević, Petar, Trifković, Jelena Đ., "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries" in XXII Congress EuroFoodChem, Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6220 .