The chemical composition of the essential oil of fresh young needles with twigs of Douglas fir (Pseudosuga menziesii Mirb. Franco) obtained by hydrodistillation were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Ten compounds, accounting for 94.26 % of the oil, were identified. The main compounds found were bornyl acetate (34.65 %), camphene (29.82 %), alpha-pinene (11.65 %) and santene (5.45 %). The antifiangal activity of the essential oil was tested against various fungal species. The minimum inhibitory concentration of Douglas fir essential oil ranged from 1.5 to 4 mu g mL(-1). The fungi most sensitive to the tested oil were Phomopsis helianthi, while Penicillium species, along with Microsporum canis, were the most resistant. Compared to the commercial fungicidal agent bifonazole, the studied essential oil demonstrated higher antifungal activity.
Hemijski sastav etarskog ulja mladih iglica sa grančicama duglazije (Pseudosuga menziesii Mirb. Franco) dobijenog destilacijom vodenom parom, analiziran je gasnom hromatografijom (GC) i kombinacijom gasne hromatografije i masene spektrometrije (GC/MS). Identifikovano je deset jedinjenja ukupne zastupljenosti 94,26 %. Kao glavne komponente su nađeni: bornil-acetat (34,65 %), kamfen (29,82 %), α-pinen (11,65 %) i santen (5,45 %). Pored toga, etarsko ulje je testirano na antifungalnu aktivnost. Etarsko ulje pokazuje mnogo bolju antifugalnu akivnost od komercijalnog fungicidnog agensa bifonazola. Minimalna inhibitorna koncentracija etarskog ulja duglazije je u opsegu od 0,6 do 1,4 μL mL-1. Najosetljiviji na testirano ulje je bio soj Phomopsis helianthi, dok sojevi Penicillium i Microsporum canis pokazuju najveću rezistentnost.
Keywords:
antifungal activity / bornyl acetate / bornyl acetate / Douglas fir / Douglas fir / essential oil / essential oil
Source:
Journal of the Serbian Chemical Society, 2009, 74, 10, 1035-1040
TY - JOUR
AU - Tešević, Vele
AU - Milosavljević, Slobodan M.
AU - Vajs, Vlatka
AU - Đorđević, Iris
AU - Soković, Marina
AU - Lavadinovic, Vera
AU - Novaković, Miroslav M.
PY - 2009
UR - https://cherry.chem.bg.ac.rs/handle/123456789/1017
AB - The chemical composition of the essential oil of fresh young needles with twigs of Douglas fir (Pseudosuga menziesii Mirb. Franco) obtained by hydrodistillation were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Ten compounds, accounting for 94.26 % of the oil, were identified. The main compounds found were bornyl acetate (34.65 %), camphene (29.82 %), alpha-pinene (11.65 %) and santene (5.45 %). The antifiangal activity of the essential oil was tested against various fungal species. The minimum inhibitory concentration of Douglas fir essential oil ranged from 1.5 to 4 mu g mL(-1). The fungi most sensitive to the tested oil were Phomopsis helianthi, while Penicillium species, along with Microsporum canis, were the most resistant. Compared to the commercial fungicidal agent bifonazole, the studied essential oil demonstrated higher antifungal activity.
AB - Hemijski sastav etarskog ulja mladih iglica sa grančicama duglazije (Pseudosuga menziesii Mirb. Franco) dobijenog destilacijom vodenom parom, analiziran je gasnom hromatografijom (GC) i kombinacijom gasne hromatografije i masene spektrometrije (GC/MS). Identifikovano je deset jedinjenja ukupne zastupljenosti 94,26 %. Kao glavne komponente su nađeni: bornil-acetat (34,65 %), kamfen (29,82 %), α-pinen (11,65 %) i santen (5,45 %). Pored toga, etarsko ulje je testirano na antifungalnu aktivnost. Etarsko ulje pokazuje mnogo bolju antifugalnu akivnost od komercijalnog fungicidnog agensa bifonazola. Minimalna inhibitorna koncentracija etarskog ulja duglazije je u opsegu od 0,6 do 1,4 μL mL-1. Najosetljiviji na testirano ulje je bio soj Phomopsis helianthi, dok sojevi Penicillium i Microsporum canis pokazuju najveću rezistentnost.
PB - Serbian Chemical Soc, Belgrade
T2 - Journal of the Serbian Chemical Society
T1 - Chemical composition and antifungal activity of the essential oil of Douglas fir (Pseudosuga menziesii Mirb. Franco) from Serbia
T1 - Hemijski sastav i antifungalna aktivnost etarskog ulja daglasove jele (Pseudosuga menziesii mirb. Franco) iz Srbije
VL - 74
IS - 10
SP - 1035
EP - 1040
DO - 10.2298/JSC0910035T
ER -
@article{
author = "Tešević, Vele and Milosavljević, Slobodan M. and Vajs, Vlatka and Đorđević, Iris and Soković, Marina and Lavadinovic, Vera and Novaković, Miroslav M.",
year = "2009",
abstract = "The chemical composition of the essential oil of fresh young needles with twigs of Douglas fir (Pseudosuga menziesii Mirb. Franco) obtained by hydrodistillation were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Ten compounds, accounting for 94.26 % of the oil, were identified. The main compounds found were bornyl acetate (34.65 %), camphene (29.82 %), alpha-pinene (11.65 %) and santene (5.45 %). The antifiangal activity of the essential oil was tested against various fungal species. The minimum inhibitory concentration of Douglas fir essential oil ranged from 1.5 to 4 mu g mL(-1). The fungi most sensitive to the tested oil were Phomopsis helianthi, while Penicillium species, along with Microsporum canis, were the most resistant. Compared to the commercial fungicidal agent bifonazole, the studied essential oil demonstrated higher antifungal activity., Hemijski sastav etarskog ulja mladih iglica sa grančicama duglazije (Pseudosuga menziesii Mirb. Franco) dobijenog destilacijom vodenom parom, analiziran je gasnom hromatografijom (GC) i kombinacijom gasne hromatografije i masene spektrometrije (GC/MS). Identifikovano je deset jedinjenja ukupne zastupljenosti 94,26 %. Kao glavne komponente su nađeni: bornil-acetat (34,65 %), kamfen (29,82 %), α-pinen (11,65 %) i santen (5,45 %). Pored toga, etarsko ulje je testirano na antifungalnu aktivnost. Etarsko ulje pokazuje mnogo bolju antifugalnu akivnost od komercijalnog fungicidnog agensa bifonazola. Minimalna inhibitorna koncentracija etarskog ulja duglazije je u opsegu od 0,6 do 1,4 μL mL-1. Najosetljiviji na testirano ulje je bio soj Phomopsis helianthi, dok sojevi Penicillium i Microsporum canis pokazuju najveću rezistentnost.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical composition and antifungal activity of the essential oil of Douglas fir (Pseudosuga menziesii Mirb. Franco) from Serbia, Hemijski sastav i antifungalna aktivnost etarskog ulja daglasove jele (Pseudosuga menziesii mirb. Franco) iz Srbije",
volume = "74",
number = "10",
pages = "1035-1040",
doi = "10.2298/JSC0910035T"
}
Tešević, V., Milosavljević, S. M., Vajs, V., Đorđević, I., Soković, M., Lavadinovic, V.,& Novaković, M. M.. (2009). Chemical composition and antifungal activity of the essential oil of Douglas fir (Pseudosuga menziesii Mirb. Franco) from Serbia. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 74(10), 1035-1040.
https://doi.org/10.2298/JSC0910035T
Tešević V, Milosavljević SM, Vajs V, Đorđević I, Soković M, Lavadinovic V, Novaković MM. Chemical composition and antifungal activity of the essential oil of Douglas fir (Pseudosuga menziesii Mirb. Franco) from Serbia. in Journal of the Serbian Chemical Society. 2009;74(10):1035-1040.
doi:10.2298/JSC0910035T .
Tešević, Vele, Milosavljević, Slobodan M., Vajs, Vlatka, Đorđević, Iris, Soković, Marina, Lavadinovic, Vera, Novaković, Miroslav M., "Chemical composition and antifungal activity of the essential oil of Douglas fir (Pseudosuga menziesii Mirb. Franco) from Serbia" in Journal of the Serbian Chemical Society, 74, no. 10 (2009):1035-1040,
https://doi.org/10.2298/JSC0910035T . .
