Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes
Abstract
This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential bene...fits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved.
Keywords:
Rubus idaeus / CBMN assay / LC/UV/ESI-TOF MS / Flavanol / EllagitanninSource:
Food and Chemical Toxicology, 2009, 47, 11, 2853-2859Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Sekundarni metaboliti samoniklih, lekovitih biljaka: izolovanje, karakterizacija i biloška aktivnost (RS-142053)
DOI: 10.1016/j.fct.2009.09.006
ISSN: 0278-6915
PubMed: 19748543
WoS: 000272013400026
Scopus: 2-s2.0-70350344384
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gođevac, Dejan AU - Tešević, Vele AU - Vajs, Vlatka AU - Milosavljević, Slobodan M. AU - Stanković, Miroslava PY - 2009 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1029 AB - This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Food and Chemical Toxicology T1 - Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes VL - 47 IS - 11 SP - 2853 EP - 2859 DO - 10.1016/j.fct.2009.09.006 ER -
@article{ author = "Gođevac, Dejan and Tešević, Vele and Vajs, Vlatka and Milosavljević, Slobodan M. and Stanković, Miroslava", year = "2009", abstract = "This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Food and Chemical Toxicology", title = "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes", volume = "47", number = "11", pages = "2853-2859", doi = "10.1016/j.fct.2009.09.006" }
Gođevac, D., Tešević, V., Vajs, V., Milosavljević, S. M.,& Stanković, M.. (2009). Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes. in Food and Chemical Toxicology Pergamon-Elsevier Science Ltd, Oxford., 47(11), 2853-2859. https://doi.org/10.1016/j.fct.2009.09.006
Gođevac D, Tešević V, Vajs V, Milosavljević SM, Stanković M. Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes. in Food and Chemical Toxicology. 2009;47(11):2853-2859. doi:10.1016/j.fct.2009.09.006 .
Gođevac, Dejan, Tešević, Vele, Vajs, Vlatka, Milosavljević, Slobodan M., Stanković, Miroslava, "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes" in Food and Chemical Toxicology, 47, no. 11 (2009):2853-2859, https://doi.org/10.1016/j.fct.2009.09.006 . .