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Ispitivanje stabilnosti vakcine kućne prašine za sublingvalnu imunoterapiju

dc.creatorBurazer, Lidija M.
dc.creatorMilovanovic, Katarina
dc.creatorĆirković-Veličković, Tanja
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2018-11-22T00:15:53Z
dc.date.available2018-11-22T00:15:53Z
dc.date.issued2010
dc.identifier.issn0352-5139
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1045
dc.description.abstractAllergen-specific immunotherapy with house dust mite (HDM) allergen extracts can effectively alleviate the symptoms of allergic rhinitis and asthma. The efficacy of the immunotherapeutic treatment is highly dependent on the quality of house dust mite vaccines. This study was performed to assess the stability of house dust mite allergen vaccines prepared for sublingual immunotherapy. Lyophilized Dermatophagoides pteronyssinus (Dpt) mite bodies were the starting material for the production of sublingual vaccines in four therapeutic concentrations. The stability of the extract for vaccine production, which was stored below 4 degrees C for one month, showed consistence in the protein profile in SDS PAGE. ELISA-inhibition showed that the potencies of Dpt vaccines during a 12 month period were to 65-80 % preserved at all analyzed therapeutic concentrations. This study showed that glycerinated Dpt vaccines stored at 4 degrees C preserved their IgE-binding potential during a 12 month period, implying their suitability for sublingual immunotherapeutic treatment of HDM allergy.en
dc.description.abstractAlergen-specifična imunoterapija predstavlja postupak koji može da promeni tok bolesti kod alergijskog rinitisa i astme. Kvalitet vakcine pripremljene od kućnih grinja značajno utiče na efikasnost imunoterapeutskog tretmana. Ispitivanje je imalo za cilj procenu stabilnosti vakcine kućnih grinja namenjene za sublingvalnu imunoterapiju. Telo liofilozovanih Dermatophagoides pteronyssinus grinja (Dpt-grinja) upotrebljeno je kao polazni materijal za proizvodnju sublingvalne vakcine u 4 različita terapeutska razblaženja. Potentnost Dpt vakcina praćena ELISA-inhibicijom u 4 terapeutska razblaženja, nakon 12 meseci zadržan je u svim razblaženjima u opsegu 65-80%. Ispitivanje je pokazalo da glicerolom stabilizovane Dpt vakcine za subligvalnu imunoterapiju, uz čuvanje na 4°C zadržavaju alergeni potencijal ( gt 65%) nakon 12 meseci od njihove pripreme, i kao takve mogu se koristiti za tretman alergije na kućne grinje.sr
dc.publisherSerbian Chemical Soc, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjectDermatophagoides pteronyssinusen
dc.subjectallergen extracten
dc.subjectallergen extracten
dc.subjectvaccinesen
dc.subjectvaccinesen
dc.subjectELISA inhibitionen
dc.subjectELISA inhibitionen
dc.subjectstabilityen
dc.subjectstabilityen
dc.titleStability evaluation of house dust mite vaccines for sublingual immunotherapyen
dc.titleIspitivanje stabilnosti vakcine kućne prašine za sublingvalnu imunoterapijusr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractЋирковић-Величковић, Тања; Гавровић-Јанкуловић, Марија; Миловановиц, Катарина; Буразер, Лидија; Испитивање стабилности вакцине кућне прашине за сублингвалну имунотерапију; Испитивање стабилности вакцине кућне прашине за сублингвалну имунотерапију;
dc.citation.volume75
dc.citation.issue1
dc.citation.spage19
dc.citation.epage26
dc.identifier.wos000274586300003
dc.identifier.doi10.2298/JSC1001019B
dc.citation.other75(1): 19-26
dc.citation.rankM23
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-77950951080
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/8250/1043.pdf
dc.identifier.rcubKon_2045


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