The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model
2010
Authors
Laketa, DanijelaBogdanović, Jelena
Prodanović, Radivoje
Kalauzi, Aleksandar
Radotić, Ksenija
Article (Published version)
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We studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can ...be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active.
Keywords:
pH-related POD activity / Mathematical model / Picea omorika (Pane.) Purkinye / Peroxidase fractionsSource:
General Physiology and Biophysics, 2010, 29, 2, 122-128Publisher:
- General Physiol And Biophysics, Bratislava
Funding / projects:
DOI: 10.4149/gpb_2010_02_122
ISSN: 0231-5882
PubMed: 20577023
WoS: 000279094900002
Scopus: 2-s2.0-77954127434
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Laketa, Danijela AU - Bogdanović, Jelena AU - Prodanović, Radivoje AU - Kalauzi, Aleksandar AU - Radotić, Ksenija PY - 2010 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1089 AB - We studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active. PB - General Physiol And Biophysics, Bratislava T2 - General Physiology and Biophysics T1 - The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model VL - 29 IS - 2 SP - 122 EP - 128 DO - 10.4149/gpb_2010_02_122 ER -
@article{ author = "Laketa, Danijela and Bogdanović, Jelena and Prodanović, Radivoje and Kalauzi, Aleksandar and Radotić, Ksenija", year = "2010", abstract = "We studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active.", publisher = "General Physiol And Biophysics, Bratislava", journal = "General Physiology and Biophysics", title = "The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model", volume = "29", number = "2", pages = "122-128", doi = "10.4149/gpb_2010_02_122" }
Laketa, D., Bogdanović, J., Prodanović, R., Kalauzi, A.,& Radotić, K.. (2010). The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model. in General Physiology and Biophysics General Physiol And Biophysics, Bratislava., 29(2), 122-128. https://doi.org/10.4149/gpb_2010_02_122
Laketa D, Bogdanović J, Prodanović R, Kalauzi A, Radotić K. The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model. in General Physiology and Biophysics. 2010;29(2):122-128. doi:10.4149/gpb_2010_02_122 .
Laketa, Danijela, Bogdanović, Jelena, Prodanović, Radivoje, Kalauzi, Aleksandar, Radotić, Ksenija, "The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model" in General Physiology and Biophysics, 29, no. 2 (2010):122-128, https://doi.org/10.4149/gpb_2010_02_122 . .