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dc.creatorLaketa, Danijela
dc.creatorBogdanović, Jelena
dc.creatorProdanović, Radivoje
dc.creatorKalauzi, Aleksandar
dc.creatorRadotić, Ksenija
dc.date.accessioned2018-11-22T00:16:33Z
dc.date.available2018-11-22T00:16:33Z
dc.date.issued2010
dc.identifier.issn0231-5882
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1089
dc.description.abstractWe studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active.en
dc.publisherGeneral Physiol And Biophysics, Bratislava
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143043/RS//
dc.rightsopenAccess
dc.sourceGeneral Physiology and Biophysics
dc.subjectpH-related POD activityen
dc.subjectMathematical modelen
dc.subjectPicea omorika (Pane.) Purkinyeen
dc.subjectPeroxidase fractionsen
dc.titleThe effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical modelen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРадотиц, Ксенија; Калаузи, Aлександар; Лакета, Данијела; Продановић, Радивоје; Богдановиц, Јелена;
dc.citation.volume29
dc.citation.issue2
dc.citation.spage122
dc.citation.epage128
dc.identifier.wos000279094900002
dc.identifier.doi10.4149/gpb_2010_02_122
dc.citation.other29(2): 122-128
dc.citation.rankM23
dc.identifier.pmid20577023
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-77954127434
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/8290/1087.pdf
dc.identifier.rcubKon_2089


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