Yeast cell wall invertase (CWI) was modified with dimethyl suberimidate, glutaraldehyde, formaldehyde, and sodium periodate. Retained activity after modification was 45% for CWI modified with formaldehyde, 77% for CWI modified with sodium periodate, 80% for CWI modified with glutaraldehyde, and 115% for CWI modified with dimethyl suberimidate. Chemically modified and native CWIs showed significantly broad pH stability (pH 3-11), whereas after incubations at 50, 60, and 70 degrees C, CWI modified with glutaraldehyde showed the highest thermostability. Optimum pH for CWI modified with glutaraldehyde was between 4 and 5, whereas optimum temperature was at 60 degrees C. Comparison to CWI modified with glutaraldehyde after immobilization within alginate beads showed broader pH optimum (4.0-5.5) as well as broader temperature optimum (55-70 degrees C). Column bed reactor packed with the immobilized CWI modified with glutaraldehyde was successfully used for the 95% inversion of 60% (w/w) sucr...ose at the flow rate of 3 bed volumes per hour, pH 4.9, and 45 degrees C. A 1 month productivity of 3844 kg of inverted sugar/kg of the immobilisate was obtained.
TY - JOUR
AU - Vujčić, Zoran
AU - Milovanovic, Aleksandra
AU - Božić, Nataša
AU - Dojnov, Biljana
AU - Vujčić, Miroslava
AU - Anđelković, Uroš
AU - Lončar, Nikola L.
PY - 2010
UR - https://cherry.chem.bg.ac.rs/handle/123456789/1133
AB - Yeast cell wall invertase (CWI) was modified with dimethyl suberimidate, glutaraldehyde, formaldehyde, and sodium periodate. Retained activity after modification was 45% for CWI modified with formaldehyde, 77% for CWI modified with sodium periodate, 80% for CWI modified with glutaraldehyde, and 115% for CWI modified with dimethyl suberimidate. Chemically modified and native CWIs showed significantly broad pH stability (pH 3-11), whereas after incubations at 50, 60, and 70 degrees C, CWI modified with glutaraldehyde showed the highest thermostability. Optimum pH for CWI modified with glutaraldehyde was between 4 and 5, whereas optimum temperature was at 60 degrees C. Comparison to CWI modified with glutaraldehyde after immobilization within alginate beads showed broader pH optimum (4.0-5.5) as well as broader temperature optimum (55-70 degrees C). Column bed reactor packed with the immobilized CWI modified with glutaraldehyde was successfully used for the 95% inversion of 60% (w/w) sucrose at the flow rate of 3 bed volumes per hour, pH 4.9, and 45 degrees C. A 1 month productivity of 3844 kg of inverted sugar/kg of the immobilisate was obtained.
PB - Amer Chemical Soc, Washington
T2 - Journal of Agricultural and Food Chemistry
T1 - Immobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugar
VL - 58
IS - 22
SP - 11896
EP - 11900
DO - 10.1021/jf101836r
ER -
@article{
author = "Vujčić, Zoran and Milovanovic, Aleksandra and Božić, Nataša and Dojnov, Biljana and Vujčić, Miroslava and Anđelković, Uroš and Lončar, Nikola L.",
year = "2010",
abstract = "Yeast cell wall invertase (CWI) was modified with dimethyl suberimidate, glutaraldehyde, formaldehyde, and sodium periodate. Retained activity after modification was 45% for CWI modified with formaldehyde, 77% for CWI modified with sodium periodate, 80% for CWI modified with glutaraldehyde, and 115% for CWI modified with dimethyl suberimidate. Chemically modified and native CWIs showed significantly broad pH stability (pH 3-11), whereas after incubations at 50, 60, and 70 degrees C, CWI modified with glutaraldehyde showed the highest thermostability. Optimum pH for CWI modified with glutaraldehyde was between 4 and 5, whereas optimum temperature was at 60 degrees C. Comparison to CWI modified with glutaraldehyde after immobilization within alginate beads showed broader pH optimum (4.0-5.5) as well as broader temperature optimum (55-70 degrees C). Column bed reactor packed with the immobilized CWI modified with glutaraldehyde was successfully used for the 95% inversion of 60% (w/w) sucrose at the flow rate of 3 bed volumes per hour, pH 4.9, and 45 degrees C. A 1 month productivity of 3844 kg of inverted sugar/kg of the immobilisate was obtained.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Immobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugar",
volume = "58",
number = "22",
pages = "11896-11900",
doi = "10.1021/jf101836r"
}
Vujčić, Z., Milovanovic, A., Božić, N., Dojnov, B., Vujčić, M., Anđelković, U.,& Lončar, N. L.. (2010). Immobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugar. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 58(22), 11896-11900.
https://doi.org/10.1021/jf101836r
Vujčić Z, Milovanovic A, Božić N, Dojnov B, Vujčić M, Anđelković U, Lončar NL. Immobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugar. in Journal of Agricultural and Food Chemistry. 2010;58(22):11896-11900.
doi:10.1021/jf101836r .
Vujčić, Zoran, Milovanovic, Aleksandra, Božić, Nataša, Dojnov, Biljana, Vujčić, Miroslava, Anđelković, Uroš, Lončar, Nikola L., "Immobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugar" in Journal of Agricultural and Food Chemistry, 58, no. 22 (2010):11896-11900,
https://doi.org/10.1021/jf101836r . .
