Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale
Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou
2010
Аутори
Beškoski, VladimirTakić, Miloš
Milic, Jelena
Ilić, Mila V.
Gojgić-Cvijović, Gordana D.
Jovančićević, Branimir
Vrvić, Miroslav M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m(3)). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/reinoculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg(-1) day(-1) for the aliphatic fraction, 6 mg kg(-1) day(-1) for the aromatic fraction and 3 mg kg(-1) day(-1) for the nitroge...n-sulphur-oxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyse isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type.
Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji met...odom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa.
Кључне речи:
mazut / bioremediation / bioremediation / field experiment / field experiment / zymogenous microbial consortia / zymogenous microbial consortia / isoprenoids / isoprenoids / steranes and terpanes / steranes and terpanesИзвор:
Journal of the Serbian Chemical Society, 2010, 75, 11, 1605-1616Издавач:
- Serbian Chemical Soc, Belgrade
Финансирање / пројекти:
- Биомаса и метаболизам неких микроорганизама као извор широко употребљивих производа и биохемијских реакција (RS-MESTD-MPN2006-2010-142018)
- Производни мобилни биореактор и добијање биомасе микроорганизама за биоремедијацију (RS-MESTD-MPN2006-2010-20131)
DOI: 10.2298/JSC100505091B
ISSN: 0352-5139
WoS: 000285214100013
Scopus: 2-s2.0-78649614679
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Beškoski, Vladimir AU - Takić, Miloš AU - Milic, Jelena AU - Ilić, Mila V. AU - Gojgić-Cvijović, Gordana D. AU - Jovančićević, Branimir AU - Vrvić, Miroslav M. PY - 2010 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1139 AB - This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m(3)). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/reinoculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg(-1) day(-1) for the aliphatic fraction, 6 mg kg(-1) day(-1) for the aromatic fraction and 3 mg kg(-1) day(-1) for the nitrogen-sulphur-oxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyse isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type. AB - Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji metodom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa. PB - Serbian Chemical Soc, Belgrade T2 - Journal of the Serbian Chemical Society T1 - Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale T1 - Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou VL - 75 IS - 11 SP - 1605 EP - 1616 DO - 10.2298/JSC100505091B ER -
@article{ author = "Beškoski, Vladimir and Takić, Miloš and Milic, Jelena and Ilić, Mila V. and Gojgić-Cvijović, Gordana D. and Jovančićević, Branimir and Vrvić, Miroslav M.", year = "2010", abstract = "This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m(3)). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/reinoculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg(-1) day(-1) for the aliphatic fraction, 6 mg kg(-1) day(-1) for the aromatic fraction and 3 mg kg(-1) day(-1) for the nitrogen-sulphur-oxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyse isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type., Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji metodom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa.", publisher = "Serbian Chemical Soc, Belgrade", journal = "Journal of the Serbian Chemical Society", title = "Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale, Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou", volume = "75", number = "11", pages = "1605-1616", doi = "10.2298/JSC100505091B" }
Beškoski, V., Takić, M., Milic, J., Ilić, M. V., Gojgić-Cvijović, G. D., Jovančićević, B.,& Vrvić, M. M.. (2010). Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale. in Journal of the Serbian Chemical Society Serbian Chemical Soc, Belgrade., 75(11), 1605-1616. https://doi.org/10.2298/JSC100505091B
Beškoski V, Takić M, Milic J, Ilić MV, Gojgić-Cvijović GD, Jovančićević B, Vrvić MM. Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale. in Journal of the Serbian Chemical Society. 2010;75(11):1605-1616. doi:10.2298/JSC100505091B .
Beškoski, Vladimir, Takić, Miloš, Milic, Jelena, Ilić, Mila V., Gojgić-Cvijović, Gordana D., Jovančićević, Branimir, Vrvić, Miroslav M., "Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale" in Journal of the Serbian Chemical Society, 75, no. 11 (2010):1605-1616, https://doi.org/10.2298/JSC100505091B . .