Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity
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Zdunić, GordanaGođevac, Dejan
Šavikin, Katarina
Novaković, Miroslav M.
Milosavljević, Slobodan M.
Petrović, Silvana
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The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phen...olic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.
Keywords:
Hypericum richeri / phenolics / HPLC-DAD / on-line HPLC-DPPH / antioxidant capacitySource:
Natural Product Research, 2011, 25, 3, 175-187Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Biljni proizvodi u preventivi i terapiji nezaraznih hroničnih oboljenja kod ljudi (RS-20035)
- Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-143012)
DOI: 10.1080/14786410802401390
ISSN: 1478-6419
PubMed: 19764002
WoS: 000286894900001
Scopus: 2-s2.0-79551671472
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Zdunić, Gordana AU - Gođevac, Dejan AU - Šavikin, Katarina AU - Novaković, Miroslav M. AU - Milosavljević, Slobodan M. AU - Petrović, Silvana PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1148 AB - The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri. PB - Taylor & Francis Ltd, Abingdon T2 - Natural Product Research T1 - Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity VL - 25 IS - 3 SP - 175 EP - 187 DO - 10.1080/14786410802401390 ER -
@article{ author = "Zdunić, Gordana and Gođevac, Dejan and Šavikin, Katarina and Novaković, Miroslav M. and Milosavljević, Slobodan M. and Petrović, Silvana", year = "2011", abstract = "The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Natural Product Research", title = "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity", volume = "25", number = "3", pages = "175-187", doi = "10.1080/14786410802401390" }
Zdunić, G., Gođevac, D., Šavikin, K., Novaković, M. M., Milosavljević, S. M.,& Petrović, S.. (2011). Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research Taylor & Francis Ltd, Abingdon., 25(3), 175-187. https://doi.org/10.1080/14786410802401390
Zdunić G, Gođevac D, Šavikin K, Novaković MM, Milosavljević SM, Petrović S. Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research. 2011;25(3):175-187. doi:10.1080/14786410802401390 .
Zdunić, Gordana, Gođevac, Dejan, Šavikin, Katarina, Novaković, Miroslav M., Milosavljević, Slobodan M., Petrović, Silvana, "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity" in Natural Product Research, 25, no. 3 (2011):175-187, https://doi.org/10.1080/14786410802401390 . .