Method for monitoring of the protein amino group changes during carbonylation
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2011
Authors
Aćimović, Jelena M.Jovanović, Vesna B.
Veselinovic, Milica R.
Serckovic, Vesna Dimitrijevic
Mandić, Ljuba M.
Article (Published version)
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Objectives: Carbonylation of the protein amino, guanidino and thiol groups inane of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. Design and methods: The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation in vitro with methylglyoxal and in vivo in 13 patients with type 2 diabetes and 20 healthy persons. Results: The method is simple, fast, precise (RSD in the range of 1.2-1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p lt 0.01) in comparison with a control group. Conclusion: The method developed is suitable for quantification of protein amino groups during in vitro carbonylation as well as for clinical practice. (C) 2011 The Canadian Society of Clinical Chemists.... Published by Elsevier Inc. All rights reserved.
Keywords:
Protein amino group / Carbonylation / Methylglyoxal / Diabetes / Spectrophotometric determination with p-benzoquinoneSource:
Clinical Biochemistry, 2011, 44, 12, 994-999Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1016/j.clinbiochem.2011.05.019
ISSN: 0009-9120
PubMed: 21640090
WoS: 000293043400011
Scopus: 2-s2.0-79960324403
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Aćimović, Jelena M. AU - Jovanović, Vesna B. AU - Veselinovic, Milica R. AU - Serckovic, Vesna Dimitrijevic AU - Mandić, Ljuba M. PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1179 AB - Objectives: Carbonylation of the protein amino, guanidino and thiol groups inane of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. Design and methods: The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation in vitro with methylglyoxal and in vivo in 13 patients with type 2 diabetes and 20 healthy persons. Results: The method is simple, fast, precise (RSD in the range of 1.2-1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p lt 0.01) in comparison with a control group. Conclusion: The method developed is suitable for quantification of protein amino groups during in vitro carbonylation as well as for clinical practice. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Clinical Biochemistry T1 - Method for monitoring of the protein amino group changes during carbonylation VL - 44 IS - 12 SP - 994 EP - 999 DO - 10.1016/j.clinbiochem.2011.05.019 ER -
@article{ author = "Aćimović, Jelena M. and Jovanović, Vesna B. and Veselinovic, Milica R. and Serckovic, Vesna Dimitrijevic and Mandić, Ljuba M.", year = "2011", abstract = "Objectives: Carbonylation of the protein amino, guanidino and thiol groups inane of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. Design and methods: The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation in vitro with methylglyoxal and in vivo in 13 patients with type 2 diabetes and 20 healthy persons. Results: The method is simple, fast, precise (RSD in the range of 1.2-1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p lt 0.01) in comparison with a control group. Conclusion: The method developed is suitable for quantification of protein amino groups during in vitro carbonylation as well as for clinical practice. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Clinical Biochemistry", title = "Method for monitoring of the protein amino group changes during carbonylation", volume = "44", number = "12", pages = "994-999", doi = "10.1016/j.clinbiochem.2011.05.019" }
Aćimović, J. M., Jovanović, V. B., Veselinovic, M. R., Serckovic, V. D.,& Mandić, L. M.. (2011). Method for monitoring of the protein amino group changes during carbonylation. in Clinical Biochemistry Pergamon-Elsevier Science Ltd, Oxford., 44(12), 994-999. https://doi.org/10.1016/j.clinbiochem.2011.05.019
Aćimović JM, Jovanović VB, Veselinovic MR, Serckovic VD, Mandić LM. Method for monitoring of the protein amino group changes during carbonylation. in Clinical Biochemistry. 2011;44(12):994-999. doi:10.1016/j.clinbiochem.2011.05.019 .
Aćimović, Jelena M., Jovanović, Vesna B., Veselinovic, Milica R., Serckovic, Vesna Dimitrijevic, Mandić, Ljuba M., "Method for monitoring of the protein amino group changes during carbonylation" in Clinical Biochemistry, 44, no. 12 (2011):994-999, https://doi.org/10.1016/j.clinbiochem.2011.05.019 . .