Process Analysis of the Conversion of Styrene to Biomass and Medium Chain Length Polyhydroxyalkanoate in a Two-Phase Bioreactor
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Аутори
Nikodinović-Runić, JasminaCasey, Eoin
Duane, Gearoid F.
Mitić, Dragana
Hume, Aisling R.
Kenny, Shane T.
O'Connor, Kevin E.
Чланак у часопису (Објављена верзија)
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Приказ свих података о документуАпстракт
The improvement and modeling of a process for the supply of the volatile aromatic hydrocarbon, styrene, to a fermentor for increased biomass production of the medium chain length polyhydroxyalkanoate (mcl-PHA) accumulating bacterium Pseudomonas putida CA-3 was investigated. Fed-batch experiments were undertaken using different methods to provide the styrene. Initial experiments where styrene was supplied as a liquid to the bioreactor had detrimental effects on cell growth and inhibited PHA polymer accumulation. By changing the feed of gaseous styrene to liquid styrene through the air sparger a 5.4-fold increase in cell dry-weight was achieved (total of 10.56 g L(-1)) which corresponds to a fourfold improvement in PHA production (3.36 g L(-1)) compared to previous studies performed in our laboratory (0.82 g L(-1)). In addition this final improved feeding strategy reduced the release of styrene from the fermentor 50-fold compared to initial experiments (0.12mL total styrene released per ...48 h run). An unstructured kinetic model was developed to describe cell growth along with substrate and oxygen utilization. The formation of dispersed gas (air) and liquid (styrene) phases in the medium and the transfer of styrene between the aqueous and dispersed liquid droplet phases was also modeled. The model provided a detailed description of these phase transitions and helped explain how the feeding strategy led to improved process performance in terms of final biomass levels. It also highlighted the key factors to be considered during further process improvement. Biotechnol. Bioeng. 2011; 108: 2447-2455. (C) 2011 Wiley Periodicals, Inc.
Кључне речи:
styrene / unstructured kinetic model / two phase system / polyhydroxyalkanoate / Pseudomonas putida CA-3Извор:
Biotechnology and Bioengineering, 2011, 108, 10, 2447-2455Издавач:
- Wiley-Blackwell, Malden
DOI: 10.1002/bit.23187
ISSN: 0006-3592
PubMed: 21520026
WoS: 000294107700020
Scopus: 2-s2.0-80051784632
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Nikodinović-Runić, Jasmina AU - Casey, Eoin AU - Duane, Gearoid F. AU - Mitić, Dragana AU - Hume, Aisling R. AU - Kenny, Shane T. AU - O'Connor, Kevin E. PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1193 AB - The improvement and modeling of a process for the supply of the volatile aromatic hydrocarbon, styrene, to a fermentor for increased biomass production of the medium chain length polyhydroxyalkanoate (mcl-PHA) accumulating bacterium Pseudomonas putida CA-3 was investigated. Fed-batch experiments were undertaken using different methods to provide the styrene. Initial experiments where styrene was supplied as a liquid to the bioreactor had detrimental effects on cell growth and inhibited PHA polymer accumulation. By changing the feed of gaseous styrene to liquid styrene through the air sparger a 5.4-fold increase in cell dry-weight was achieved (total of 10.56 g L(-1)) which corresponds to a fourfold improvement in PHA production (3.36 g L(-1)) compared to previous studies performed in our laboratory (0.82 g L(-1)). In addition this final improved feeding strategy reduced the release of styrene from the fermentor 50-fold compared to initial experiments (0.12mL total styrene released per 48 h run). An unstructured kinetic model was developed to describe cell growth along with substrate and oxygen utilization. The formation of dispersed gas (air) and liquid (styrene) phases in the medium and the transfer of styrene between the aqueous and dispersed liquid droplet phases was also modeled. The model provided a detailed description of these phase transitions and helped explain how the feeding strategy led to improved process performance in terms of final biomass levels. It also highlighted the key factors to be considered during further process improvement. Biotechnol. Bioeng. 2011; 108: 2447-2455. (C) 2011 Wiley Periodicals, Inc. PB - Wiley-Blackwell, Malden T2 - Biotechnology and Bioengineering T1 - Process Analysis of the Conversion of Styrene to Biomass and Medium Chain Length Polyhydroxyalkanoate in a Two-Phase Bioreactor VL - 108 IS - 10 SP - 2447 EP - 2455 DO - 10.1002/bit.23187 ER -
@article{ author = "Nikodinović-Runić, Jasmina and Casey, Eoin and Duane, Gearoid F. and Mitić, Dragana and Hume, Aisling R. and Kenny, Shane T. and O'Connor, Kevin E.", year = "2011", abstract = "The improvement and modeling of a process for the supply of the volatile aromatic hydrocarbon, styrene, to a fermentor for increased biomass production of the medium chain length polyhydroxyalkanoate (mcl-PHA) accumulating bacterium Pseudomonas putida CA-3 was investigated. Fed-batch experiments were undertaken using different methods to provide the styrene. Initial experiments where styrene was supplied as a liquid to the bioreactor had detrimental effects on cell growth and inhibited PHA polymer accumulation. By changing the feed of gaseous styrene to liquid styrene through the air sparger a 5.4-fold increase in cell dry-weight was achieved (total of 10.56 g L(-1)) which corresponds to a fourfold improvement in PHA production (3.36 g L(-1)) compared to previous studies performed in our laboratory (0.82 g L(-1)). In addition this final improved feeding strategy reduced the release of styrene from the fermentor 50-fold compared to initial experiments (0.12mL total styrene released per 48 h run). An unstructured kinetic model was developed to describe cell growth along with substrate and oxygen utilization. The formation of dispersed gas (air) and liquid (styrene) phases in the medium and the transfer of styrene between the aqueous and dispersed liquid droplet phases was also modeled. The model provided a detailed description of these phase transitions and helped explain how the feeding strategy led to improved process performance in terms of final biomass levels. It also highlighted the key factors to be considered during further process improvement. Biotechnol. Bioeng. 2011; 108: 2447-2455. (C) 2011 Wiley Periodicals, Inc.", publisher = "Wiley-Blackwell, Malden", journal = "Biotechnology and Bioengineering", title = "Process Analysis of the Conversion of Styrene to Biomass and Medium Chain Length Polyhydroxyalkanoate in a Two-Phase Bioreactor", volume = "108", number = "10", pages = "2447-2455", doi = "10.1002/bit.23187" }
Nikodinović-Runić, J., Casey, E., Duane, G. F., Mitić, D., Hume, A. R., Kenny, S. T.,& O'Connor, K. E.. (2011). Process Analysis of the Conversion of Styrene to Biomass and Medium Chain Length Polyhydroxyalkanoate in a Two-Phase Bioreactor. in Biotechnology and Bioengineering Wiley-Blackwell, Malden., 108(10), 2447-2455. https://doi.org/10.1002/bit.23187
Nikodinović-Runić J, Casey E, Duane GF, Mitić D, Hume AR, Kenny ST, O'Connor KE. Process Analysis of the Conversion of Styrene to Biomass and Medium Chain Length Polyhydroxyalkanoate in a Two-Phase Bioreactor. in Biotechnology and Bioengineering. 2011;108(10):2447-2455. doi:10.1002/bit.23187 .
Nikodinović-Runić, Jasmina, Casey, Eoin, Duane, Gearoid F., Mitić, Dragana, Hume, Aisling R., Kenny, Shane T., O'Connor, Kevin E., "Process Analysis of the Conversion of Styrene to Biomass and Medium Chain Length Polyhydroxyalkanoate in a Two-Phase Bioreactor" in Biotechnology and Bioengineering, 108, no. 10 (2011):2447-2455, https://doi.org/10.1002/bit.23187 . .