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Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel

Authorized Users Only
2011
Authors
Milutinovic, Milena
Sallard, Sebastien
Manojlović, Dragan D.
Mano, Nicolas
Šojić, Nešo
Article (Published version)
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Abstract
In this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensi...ty of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.

Keywords:
Glucose / NADH / Dehydrogenase / Electrochemiluminescence / Redox hydrogel
Source:
Bioelectrochemistry, 2011, 82, 1, 63-68
Publisher:
  • Elsevier Science Sa, Lausanne
Funding / projects:
  • CNRS (Chemistry department)
  • Ministere des Affaires Etrangeres et Europeennes
  • Centre culturel francais de Belgrade
  • Ministry of Science and Technological Development (Republic of Serbia)

DOI: 10.1016/j.bioelechem.2011.05.005

ISSN: 1567-5394

PubMed: 21733762

WoS: 000295301700008

Scopus: 2-s2.0-80051825170
[ Google Scholar ]
22
21
URI
https://cherry.chem.bg.ac.rs/handle/123456789/1203
Collections
  • Publikacije
Institution/Community
Hemijski fakultet
TY  - JOUR
AU  - Milutinovic, Milena
AU  - Sallard, Sebastien
AU  - Manojlović, Dragan D.
AU  - Mano, Nicolas
AU  - Šojić, Nešo
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1203
AB  - In this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensity of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Sa, Lausanne
T2  - Bioelectrochemistry
T1  - Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel
VL  - 82
IS  - 1
SP  - 63
EP  - 68
DO  - 10.1016/j.bioelechem.2011.05.005
UR  - Kon_2225
ER  - 
@article{
author = "Milutinovic, Milena and Sallard, Sebastien and Manojlović, Dragan D. and Mano, Nicolas and Šojić, Nešo",
year = "2011",
abstract = "In this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensity of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Bioelectrochemistry",
title = "Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel",
volume = "82",
number = "1",
pages = "63-68",
doi = "10.1016/j.bioelechem.2011.05.005",
url = "Kon_2225"
}
Milutinovic, M., Sallard, S., Manojlović, D. D., Mano, N.,& Šojić, N.. (2011). Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel. in Bioelectrochemistry
Elsevier Science Sa, Lausanne., 82(1), 63-68.
https://doi.org/10.1016/j.bioelechem.2011.05.005
Kon_2225
Milutinovic M, Sallard S, Manojlović DD, Mano N, Šojić N. Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel. in Bioelectrochemistry. 2011;82(1):63-68.
doi:10.1016/j.bioelechem.2011.05.005
Kon_2225 .
Milutinovic, Milena, Sallard, Sebastien, Manojlović, Dragan D., Mano, Nicolas, Šojić, Nešo, "Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel" in Bioelectrochemistry, 82, no. 1 (2011):63-68,
https://doi.org/10.1016/j.bioelechem.2011.05.005 .,
Kon_2225 .

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