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dc.creatorMilutinovic, Milena
dc.creatorSallard, Sebastien
dc.creatorManojlović, Dragan D.
dc.creatorMano, Nicolas
dc.creatorŠojić, Nešo
dc.date.accessioned2018-11-22T00:18:52Z
dc.date.available2018-11-22T00:18:52Z
dc.date.issued2011
dc.identifier.issn1567-5394
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1203
dc.description.abstractIn this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensity of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.en
dc.publisherElsevier Science Sa, Lausanne
dc.relationCNRS (Chemistry department)
dc.relationMinistere des Affaires Etrangeres et Europeennes
dc.relationCentre culturel francais de Belgrade
dc.relationMinistry of Science and Technological Development (Republic of Serbia)
dc.rightsrestrictedAccess
dc.sourceBioelectrochemistry
dc.subjectGlucoseen
dc.subjectNADHen
dc.subjectDehydrogenaseen
dc.subjectElectrochemiluminescenceen
dc.subjectRedox hydrogelen
dc.titleGlucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogelen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМано, Ницолас; Сојиц, Несо; Саллард, Себастиен; Милутиновиц, Милена; Манојловић, Драган;
dc.citation.volume82
dc.citation.issue1
dc.citation.spage63
dc.citation.epage68
dc.identifier.wos000295301700008
dc.identifier.doi10.1016/j.bioelechem.2011.05.005
dc.citation.other82(1): 63-68
dc.citation.rankM21
dc.identifier.pmid21733762
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-80051825170
dc.identifier.rcubKon_2225


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