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dc.creatorLončar, Nikola L.
dc.creatorVujčić, Zoran
dc.date.accessioned2018-11-22T00:19:15Z
dc.date.available2018-11-22T00:19:15Z
dc.date.issued2011
dc.identifier.issn0304-3894
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1228
dc.description.abstractHalogenated compounds represent one of the most dangerous environmental pollutants, due to their widespread usage as biocides, fungicides, disinfectants, solvent and other industrial chemicals. Immobilization of a protein through coordinate bonds formed with divalent metal ions is becoming an attractive method due to its reversible nature, since the protein may be easily removed from the support matrix through interruption of the protein-metal bond hence giving inherently cleaner and cheaper technology for wastewater treatment. We have synthesized novel 'tentacle' carrier (TC) and used it for immobilization of partially purified potato polyphenol oxidase (PPO). The obtained biocatalyst TC-PPO showed pH optimum at 7.0-8.0 and temperature optimum at 25 degrees C. Immobilized PPO shows almost 100% of activity at 0 degrees C. TC-PPO was more resistant to the denaturation induced by sodium dodecyl sulphate (SDS) detergent as compared to its soluble counterpart and was even slightly activated at SDS concentration of 1%. TC-PPO was tested in the batch reactor for 4-chlorophenol and 4-bromophenol removal. More than 90% removal was achieved for both halogenophenols at concentration of 100 mg/L from aqueous solution. For both halogenophenols TC-PPO works with over 90% removal during first three cycles which decrease to 60% removal efficiency after six cycles each of 8 h duration. (C) 2011 Elsevier B.V. All rights reserved.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Hazardous Materials
dc.subjectPolyphenol oxidaseen
dc.subjectPotatoen
dc.subjectBromophenolen
dc.subjectChlorophenolen
dc.subjectImmobilizationen
dc.titleTentacle carrier for immobilization of potato phenoloxidase and its application for halogenophenols removal from aqueous solutionsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЛончар, Никола; Вујчић, Зоран;
dc.citation.volume196
dc.citation.spage73
dc.citation.epage78
dc.identifier.wos000297881100010
dc.identifier.doi10.1016/j.jhazmat.2011.08.071
dc.citation.other196: 73-78
dc.citation.rankaM21
dc.identifier.pmid21924549
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-80455174593
dc.identifier.rcubKon_2250


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