Study of the kinetic parameters for synthesis and hydrolysis of pharmacologically active salicin isomer catalyzed by baker's yeast maltase
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One of the key elements for understanding enzyme reactions is determination of its kinetic parameters. Since transglucosylation is kinetically controlled reaction, besides the reaction of synthesis, very important is the reaction of enzymatic hydrolysis of created product. Therefore, in this study, kinetic parameters for synthesis and secondary hydrolysis of pharmacologically active alpha isosalicin by baker's yeast maltase were calculated, and it was shown that specifity of maltase for hydrolysis is approximately 150 times higher then for synthesis.
Keywords:synthesis and hydrolysis of salicin isomer / catalysis by maltase / kinetic parameters
Source:Russian Journal of Physical Chemistry A, 2011, 85, 13, 2317-2321
- Maik Nauka/Interperiodica/Springer, New York