Chemical Characterization of Lippia Alba Essential Oil: An Alternative to Control Green Molds
Апстракт
The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by H-1 and C-13-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control. Essential oil yield is of 0.15% and the major components are neral (33.32%) and geranial (50.94%). The L. alba essential oil has MIC of 0.300-1.250 mg/mL and MFC of 0.600-1.250 mg/mL. Ketoconazole and Bifonazole show MIC ranging from... 0.025-0.500 to 0.100-0.200 mg/mL, and MFC ranging from 0.250-0.100 to 0.200-0.250 mg/mL, respectively. L. alba essential oil is classified as citral type and the results indicate that it is a potential alternative to synthetic fungicides.
Кључне речи:
antifungal / essential oil / neral / geranial / Lippia albaИзвор:
Brazilian Journal of Microbiology, 2011, 42, 4, 1537-1546Издавач:
- Soc Brasileira Microbiologia, Sao Paulo
Финансирање / пројекти:
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-MESTD-Basic Research (BR or ON)-172053)
- Paranaense University
DOI: 10.1590/S1517-83822011000400041
ISSN: 1517-8382
PubMed: 24031788
WoS: 000300430600041
Scopus: 2-s2.0-84857417473
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Glamočlija, Jasmina AU - Soković, Marina AU - Tešević, Vele AU - Linde, Giani Andrea AU - Colauto, Nelson Barros PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1250 AB - The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by H-1 and C-13-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control. Essential oil yield is of 0.15% and the major components are neral (33.32%) and geranial (50.94%). The L. alba essential oil has MIC of 0.300-1.250 mg/mL and MFC of 0.600-1.250 mg/mL. Ketoconazole and Bifonazole show MIC ranging from 0.025-0.500 to 0.100-0.200 mg/mL, and MFC ranging from 0.250-0.100 to 0.200-0.250 mg/mL, respectively. L. alba essential oil is classified as citral type and the results indicate that it is a potential alternative to synthetic fungicides. PB - Soc Brasileira Microbiologia, Sao Paulo T2 - Brazilian Journal of Microbiology T1 - Chemical Characterization of Lippia Alba Essential Oil: An Alternative to Control Green Molds VL - 42 IS - 4 SP - 1537 EP - 1546 DO - 10.1590/S1517-83822011000400041 ER -
@article{ author = "Glamočlija, Jasmina and Soković, Marina and Tešević, Vele and Linde, Giani Andrea and Colauto, Nelson Barros", year = "2011", abstract = "The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by H-1 and C-13-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control. Essential oil yield is of 0.15% and the major components are neral (33.32%) and geranial (50.94%). The L. alba essential oil has MIC of 0.300-1.250 mg/mL and MFC of 0.600-1.250 mg/mL. Ketoconazole and Bifonazole show MIC ranging from 0.025-0.500 to 0.100-0.200 mg/mL, and MFC ranging from 0.250-0.100 to 0.200-0.250 mg/mL, respectively. L. alba essential oil is classified as citral type and the results indicate that it is a potential alternative to synthetic fungicides.", publisher = "Soc Brasileira Microbiologia, Sao Paulo", journal = "Brazilian Journal of Microbiology", title = "Chemical Characterization of Lippia Alba Essential Oil: An Alternative to Control Green Molds", volume = "42", number = "4", pages = "1537-1546", doi = "10.1590/S1517-83822011000400041" }
Glamočlija, J., Soković, M., Tešević, V., Linde, G. A.,& Colauto, N. B.. (2011). Chemical Characterization of Lippia Alba Essential Oil: An Alternative to Control Green Molds. in Brazilian Journal of Microbiology Soc Brasileira Microbiologia, Sao Paulo., 42(4), 1537-1546. https://doi.org/10.1590/S1517-83822011000400041
Glamočlija J, Soković M, Tešević V, Linde GA, Colauto NB. Chemical Characterization of Lippia Alba Essential Oil: An Alternative to Control Green Molds. in Brazilian Journal of Microbiology. 2011;42(4):1537-1546. doi:10.1590/S1517-83822011000400041 .
Glamočlija, Jasmina, Soković, Marina, Tešević, Vele, Linde, Giani Andrea, Colauto, Nelson Barros, "Chemical Characterization of Lippia Alba Essential Oil: An Alternative to Control Green Molds" in Brazilian Journal of Microbiology, 42, no. 4 (2011):1537-1546, https://doi.org/10.1590/S1517-83822011000400041 . .