Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation
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2012
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Prikaz svih podataka o dokumentuApstrakt
Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases. (C) 2011 Elsevier Inc. All rights reserved.
Ključne reči:
alpha-Amylase / Glucoamylase / Aspergillus niger / Zymogram / SSFIzvor:
Analytical Biochemistry, 2012, 421, 2, 802-804Izdavač:
- Academic Press Inc Elsevier Science, San Diego
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-MESTD-Basic Research (BR or ON)-172048)
DOI: 10.1016/j.ab.2011.11.039
ISSN: 0003-2697
PubMed: 22206938
WoS: 000300528400067
Scopus: 2-s2.0-84859722982
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Dojnov, Biljana AU - Vujčić, Zoran PY - 2012 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1254 AB - Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases. (C) 2011 Elsevier Inc. All rights reserved. PB - Academic Press Inc Elsevier Science, San Diego T2 - Analytical Biochemistry T1 - Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation VL - 421 IS - 2 SP - 802 EP - 804 DO - 10.1016/j.ab.2011.11.039 ER -
@article{ author = "Dojnov, Biljana and Vujčić, Zoran", year = "2012", abstract = "Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases. (C) 2011 Elsevier Inc. All rights reserved.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Analytical Biochemistry", title = "Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation", volume = "421", number = "2", pages = "802-804", doi = "10.1016/j.ab.2011.11.039" }
Dojnov, B.,& Vujčić, Z.. (2012). Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation. in Analytical Biochemistry Academic Press Inc Elsevier Science, San Diego., 421(2), 802-804. https://doi.org/10.1016/j.ab.2011.11.039
Dojnov B, Vujčić Z. Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation. in Analytical Biochemistry. 2012;421(2):802-804. doi:10.1016/j.ab.2011.11.039 .
Dojnov, Biljana, Vujčić, Zoran, "Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation" in Analytical Biochemistry, 421, no. 2 (2012):802-804, https://doi.org/10.1016/j.ab.2011.11.039 . .