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Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis

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2012
Authors
Kovacevic-Filipovic, Milica
Ilić, Vesna
Vujčić, Zoran
Dojnov, Biljana
Stevanov-Pavlović, Marija
Mijacevic, Zora
Božić, Tatjana T.
Article (Published version)
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Abstract
Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows... in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content. (C) 2011 Elsevier B.V. All rights reserved.

Keywords:
Serum amyloid A / Isoforms / Subclinical mastitis / Staphylococcus aureus
Source:
Veterinary Immunology and Immunopathology, 2012, 145, 1-2, 120-128
Publisher:
  • Elsevier Science Bv, Amsterdam
Projects:
  • Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-175061)
  • Regenerative and modulatory potential of adult stem cells (RS-175062)
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
  • Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-46009)

DOI: 10.1016/j.vetimm.2011.10.015

ISSN: 0165-2427

PubMed: 22100192

WoS: 000301159200013

Scopus: 2-s2.0-84856555615
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URI
http://cherry.chem.bg.ac.rs/handle/123456789/1257
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Hemijski fakultet

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