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Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells

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2012
Authors
Čavić, Milena
Grozdanović, Milica
Bajić, Aleksandar
Srdić-Rajić, Tatjana
Andjus, Pavle R.
Gavrović-Jankulović, Marija
Article (Published version)
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Abstract
Actinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. In this study, the effect of actinidin on cellular morphology and adhesion of T84 intestinal cells was investigated. Both rounding and detachment of T84 cells were observed upon actinidin treatment. The morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. Changes of intercellular adhesion and adhesion of epithelial cells to collagen upon actinidin treatment could be responsible for the cell rounding and give rise to discontinuous breaches in the epithelial monolayer observed in this study. Actinidin's action on cell morphology, adhesion and monolayer integrity were not due to compromised viability of T84 epithelial cells, as confirmed by MTT assay and flow cytometric analysis of the cell cycle. Damage to the epithelial monolayer of the intestine induced by actinidin should be further evaluated as an important factor in the dev...elopment of kiwifruit allergy and other intestinal disorders.

Keywords:
Actinidia sp. / Kiwifruit / Actinidin / T84 cells / Intestinal disorders
Source:
Phytochemistry, 2012, 77, 46-52
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Projects:
  • Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)

DOI: 10.1016/j.phytochem.2011.12.014

ISSN: 0031-9422

PubMed: 22257750

WoS: 000303273300003

Scopus: 2-s2.0-84859163978
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URI
http://cherry.chem.bg.ac.rs/handle/123456789/1284
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