Faculty of Chemistry Repository - Cherry
University of Belgrade - Faculty of Chemistry
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   Cherry
  • Hemijski fakultet / Faculty of Chemistry
  • Publikacije / Publications
  • View Item
  •   Cherry
  • Hemijski fakultet / Faculty of Chemistry
  • Publikacije / Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae

Thumbnail
2012
1298.pdf (689.3Kb)
Authors
Pavlovic, R.
Grujić, Marica
Dojnov, Biljana
Vujčić, Miroslava
Nenadovic, Vera
Ivanovic, Jelisaveta
Vujčić, Zoran
Article (Published version)
Metadata
Show full item record
Abstract
The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven beta-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and beta-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-beta-1,4-glucanase; subsequently, the obtained fragments are broken down by beta-D-glucosidase mostly in middl...e midgut.

Keywords:
Cerambycidae / Cerambyx cerdo / Cerambyx cerdo / cellulase / cellulase / β-D-glucosidase / beta-D-glucosidase / endocellulase / endocellulase / isoforms / isoforms / zymogram / zymogram / midgut / midgut / Serbia / Serbia
Source:
Archives of biological sciences, 2012, 64, 2, 757-765
Publisher:
  • Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Funding / projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)

DOI: 10.2298/ABS1202757P

ISSN: 0354-4664

WoS: 000304339100043

Scopus: 2-s2.0-84862255096
[ Google Scholar ]
6
5
URI
https://cherry.chem.bg.ac.rs/handle/123456789/1300
Collections
  • Publikacije / Publications
Institution/Community
Hemijski fakultet / Faculty of Chemistry
TY  - JOUR
AU  - Pavlovic, R.
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Nenadovic, Vera
AU  - Ivanovic, Jelisaveta
AU  - Vujčić, Zoran
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1300
AB  - The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven beta-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and beta-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-beta-1,4-glucanase; subsequently, the obtained fragments are broken down by beta-D-glucosidase mostly in middle midgut.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae
VL  - 64
IS  - 2
SP  - 757
EP  - 765
DO  - 10.2298/ABS1202757P
ER  - 
@article{
author = "Pavlovic, R. and Grujić, Marica and Dojnov, Biljana and Vujčić, Miroslava and Nenadovic, Vera and Ivanovic, Jelisaveta and Vujčić, Zoran",
year = "2012",
abstract = "The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven beta-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and beta-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-beta-1,4-glucanase; subsequently, the obtained fragments are broken down by beta-D-glucosidase mostly in middle midgut.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae",
volume = "64",
number = "2",
pages = "757-765",
doi = "10.2298/ABS1202757P"
}
Pavlovic, R., Grujić, M., Dojnov, B., Vujčić, M., Nenadovic, V., Ivanovic, J.,& Vujčić, Z.. (2012). Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 64(2), 757-765.
https://doi.org/10.2298/ABS1202757P
Pavlovic R, Grujić M, Dojnov B, Vujčić M, Nenadovic V, Ivanovic J, Vujčić Z. Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences. 2012;64(2):757-765.
doi:10.2298/ABS1202757P .
Pavlovic, R., Grujić, Marica, Dojnov, Biljana, Vujčić, Miroslava, Nenadovic, Vera, Ivanovic, Jelisaveta, Vujčić, Zoran, "Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae" in Archives of biological sciences, 64, no. 2 (2012):757-765,
https://doi.org/10.2298/ABS1202757P . .

DSpace software copyright © 2002-2015  DuraSpace
About CHERRY - CHEmistry RepositoRY | Send Feedback

re3dataOpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CHERRY - CHEmistry RepositoRY | Send Feedback

re3dataOpenAIRERCUB