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dc.creatorVeličković, Dušan
dc.creatorDimitrijević, Aleksandra
dc.creatorBihelović, Filip
dc.creatorBezbradica, Dejan
dc.creatorKnezevic-Jugovic, Zorica
dc.creatorMilosavić, Nenad
dc.date.accessioned2018-11-22T00:21:44Z
dc.date.available2018-11-22T00:21:44Z
dc.date.issued2012
dc.identifier.issn1615-7591
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1325
dc.description.abstractNovel glucoside of physiological active vanillyl alcohol was synthesized for the first time using maltase from Saccharomyces cerevisiae as catalyst, and established its structure as 4-hydroxy-3-methoxybenzyl-alpha-d-glucopyranoside. The key reaction factors for this transglucosylation reaction were optimized using response surface methodology and the highest yield so far in maltase catalyzed transglucosylation reaction was obtained. It was found out that optimum temperature of reaction was 37 A degrees C, optimal maltose concentration was 60% (w/v), optimal pH was 6.6, and optimal concentration of vanillyl alcohol was 158 mM. Under these conditions, yield of glucoside was 90 mM with no by product formation. It was shown that this compound posses good antioxidant activity as well as stability in gastrointestinal tract. It was demonstrated that it is hydrolyzed on brush border membrane of enterocytes, so it can serve in protecting gastrointestinal system from oxidation, as well as source of anticonvulsive drug after the hydrolysis of glucoside on brush border membrane of small intestine.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/256716/EU//
dc.rightsrestrictedAccess
dc.sourceBioprocess and Biosystems Engineering
dc.subjectVanillyl alcoholen
dc.subjectGlucosideen
dc.subjectMaltaseen
dc.subjectTransglucosylationen
dc.subjectAnticonvulsiveen
dc.subjectAntioxidant activityen
dc.titleNovel glycoside of vanillyl alcohol, 4-hydroxy-3-methoxybenzyl-alpha-d-glucopyranoside: study of enzymatic synthesis, in vitro digestion and antioxidant activityen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБезбрадица, Дејан; Велицковиц, Дусан; Димитријевиц, Aлександра; Милосавиц, Ненад; Кнезевиц-Југовиц, Зорица; Бихеловић, Филип;
dc.citation.volume35
dc.citation.issue7
dc.citation.spage1107
dc.citation.epage1115
dc.identifier.wos000307514500007
dc.identifier.doi10.1007/s00449-012-0695-3
dc.citation.other35(7): 1107-1115
dc.citation.rankM21
dc.identifier.pmid22307809
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84865357405
dc.identifier.rcubKon_2349


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