Determination of Chloramphenicol by Differential Pulse Voltammetry at Carbon Paste Electrodes - the Use of Sodium Sulfite for Removal of Oxygen from Electrode Surface
Abstract
The possibility of determination of chloramphenicol by differential pulse voltammetry at four different carbon paste electrodes, in the full pH range (2-12) of Britton-Robinson (BR) buffer was investigated. Electrodes were prepared by mixing spectroscopic graphite powder or glassy carbon microbeads with mineral oil (Nujol) or tricresyl phosphate. Under optimal conditions (BR buffer pH 12, the electrode prepared from glassy carbon microbeads and tricresyl phosphate), linear calibration graph was obtained only in 10-5 M chloramphenicol concentration range. Determination of lower concentrations of chloramphenicol was complicated by irreproducible peak of oxygen from the carbon paste which overlapped with peak of chloramphenicol. Addition of sodium sulfite removed the oxygen peak without influence on the peak of chloramphenicol. Under optimal conditions (electrode paste made from glassy carbon microbeads, BR buffer pH 10 and 0.5 M sodium sulfite), straight calibration line was obtained in ...the 10(-6) and 10(-5) M chloramphenicol concentration range. Limit of determination was 5 x 10(-7) mol/l.
Keywords:
Chloramphenicol / Carbon paste electrodes / Oxygen peak / Oxygen removal / Sodium sulfite / Differential pulse voltammetrySource:
Collection of Czechoslovak Chemical Communications, 2011, 76, 5, 383-397Publisher:
- Inst Organic Chem And Biochem, Prague 6
Funding / projects:
- Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
- [SVV 2010-10-261204]
- Ministry of Education, Youth and Sports of the Czech Republic [LC 06035, MSM 0021620857, ME 10004]
DOI: 10.1135/cccc2011011
ISSN: 0010-0765
WoS: 000290280100003
Scopus: 2-s2.0-79955668919
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Pastor, Ferenc AU - Dejmkova, Hana AU - Zima, Jiri AU - Barek, Jiri PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1334 AB - The possibility of determination of chloramphenicol by differential pulse voltammetry at four different carbon paste electrodes, in the full pH range (2-12) of Britton-Robinson (BR) buffer was investigated. Electrodes were prepared by mixing spectroscopic graphite powder or glassy carbon microbeads with mineral oil (Nujol) or tricresyl phosphate. Under optimal conditions (BR buffer pH 12, the electrode prepared from glassy carbon microbeads and tricresyl phosphate), linear calibration graph was obtained only in 10-5 M chloramphenicol concentration range. Determination of lower concentrations of chloramphenicol was complicated by irreproducible peak of oxygen from the carbon paste which overlapped with peak of chloramphenicol. Addition of sodium sulfite removed the oxygen peak without influence on the peak of chloramphenicol. Under optimal conditions (electrode paste made from glassy carbon microbeads, BR buffer pH 10 and 0.5 M sodium sulfite), straight calibration line was obtained in the 10(-6) and 10(-5) M chloramphenicol concentration range. Limit of determination was 5 x 10(-7) mol/l. PB - Inst Organic Chem And Biochem, Prague 6 T2 - Collection of Czechoslovak Chemical Communications T1 - Determination of Chloramphenicol by Differential Pulse Voltammetry at Carbon Paste Electrodes - the Use of Sodium Sulfite for Removal of Oxygen from Electrode Surface VL - 76 IS - 5 SP - 383 EP - 397 DO - 10.1135/cccc2011011 ER -
@article{ author = "Pastor, Ferenc and Dejmkova, Hana and Zima, Jiri and Barek, Jiri", year = "2011", abstract = "The possibility of determination of chloramphenicol by differential pulse voltammetry at four different carbon paste electrodes, in the full pH range (2-12) of Britton-Robinson (BR) buffer was investigated. Electrodes were prepared by mixing spectroscopic graphite powder or glassy carbon microbeads with mineral oil (Nujol) or tricresyl phosphate. Under optimal conditions (BR buffer pH 12, the electrode prepared from glassy carbon microbeads and tricresyl phosphate), linear calibration graph was obtained only in 10-5 M chloramphenicol concentration range. Determination of lower concentrations of chloramphenicol was complicated by irreproducible peak of oxygen from the carbon paste which overlapped with peak of chloramphenicol. Addition of sodium sulfite removed the oxygen peak without influence on the peak of chloramphenicol. Under optimal conditions (electrode paste made from glassy carbon microbeads, BR buffer pH 10 and 0.5 M sodium sulfite), straight calibration line was obtained in the 10(-6) and 10(-5) M chloramphenicol concentration range. Limit of determination was 5 x 10(-7) mol/l.", publisher = "Inst Organic Chem And Biochem, Prague 6", journal = "Collection of Czechoslovak Chemical Communications", title = "Determination of Chloramphenicol by Differential Pulse Voltammetry at Carbon Paste Electrodes - the Use of Sodium Sulfite for Removal of Oxygen from Electrode Surface", volume = "76", number = "5", pages = "383-397", doi = "10.1135/cccc2011011" }
Pastor, F., Dejmkova, H., Zima, J.,& Barek, J.. (2011). Determination of Chloramphenicol by Differential Pulse Voltammetry at Carbon Paste Electrodes - the Use of Sodium Sulfite for Removal of Oxygen from Electrode Surface. in Collection of Czechoslovak Chemical Communications Inst Organic Chem And Biochem, Prague 6., 76(5), 383-397. https://doi.org/10.1135/cccc2011011
Pastor F, Dejmkova H, Zima J, Barek J. Determination of Chloramphenicol by Differential Pulse Voltammetry at Carbon Paste Electrodes - the Use of Sodium Sulfite for Removal of Oxygen from Electrode Surface. in Collection of Czechoslovak Chemical Communications. 2011;76(5):383-397. doi:10.1135/cccc2011011 .
Pastor, Ferenc, Dejmkova, Hana, Zima, Jiri, Barek, Jiri, "Determination of Chloramphenicol by Differential Pulse Voltammetry at Carbon Paste Electrodes - the Use of Sodium Sulfite for Removal of Oxygen from Electrode Surface" in Collection of Czechoslovak Chemical Communications, 76, no. 5 (2011):383-397, https://doi.org/10.1135/cccc2011011 . .