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Uklanjanje fenola i fenolnih derivata iz vode imobilizovanom polifenol-oksidazom iz krompira

dc.creatorLončar, Nikola L.
dc.creatorBožić, Nataša
dc.creatorAnđelković, Ivan
dc.creatorMilovanovic, Aleksandra
dc.creatorDojnov, Biljana
dc.creatorVujčić, Miroslava
dc.creatorRoglić, Goran
dc.creatorVujčić, Zoran
dc.date.accessioned2018-11-22T00:19:34Z
dc.date.available2018-11-22T00:19:34Z
dc.date.issued2011
dc.identifier.issn0352-5139
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1339
dc.description.abstractPhenols containing halogens, which tend to deactivate the aromatic nuclei, constitute a significant category of highly toxic and difficult-to-degrade pollutants, which arise from a wide variety of industries. The main purpose of this study was to obtain an inexpensive immobilized enzyme for the removal of phenols. Partially purified potato polyphenol oxidase (PPO) was immobilized onto different commercial and laboratory produced carriers. Three of the obtained biocatalysts, with the highest PPO activities, namely Eupergit C250L-PPO; Celite-PPO and CelluloseM-PPO, were tested in a batch reactor for the removal of phenol, 4-chlorophenol and 4-bromophenol. In the case of 2.5 mM substrates with Eupergit C250L-PPO, an around 45 % removal of 4-bromophenol was achieved, while the removals 4-chlorophenol and phenol were 35 and 20 %, respectively. The reusability of Eupergit C250L-PPO for the removal of 4-chlorophenol was tested. After eight repeated tests, the efficiency of 4-chlorophenol removal by Eupergit C250L-PPO immobilisate had decreased to 55 %.en
dc.description.abstractHalogenovani fenoli imaju dezaktivirano aromatično jezgro i čine značajnu kategoriju veoma toksičnih i teško razgradivih zagađivača u raznim industrijskim granama. Glavni cilj ovog rada je bio dobijanje jeftinog imobilizovanog enzima za uklanjanje fenola. Delimično prečišćena polifenol-oksidaza (PFO) iz krompira je imobilizovana na različitim komercijalnim i laboratorijski sintetizovanim nosačima. Od dobijenih biokatalizatora, tri sa najvećim aktivnostima PPO, nazvani Eupergit C250L-PFO; Celit-PFO i CelulozaM-PFO, testirani su u reaktoru za uklanjanje fenola, 4-hlorfenola i 4-bromfenola. U slučaju 2,5 mM supstrata sa Eupergit C250L-PFO, postignuto je oko 45 % razgradnje 4-bromfenola, dok su 4-hlorfenol i fenol razgrađeni 35, odnosno 20 %. Testirana je i sposobnost višestruke upotrebe Eupergit C250L-PFO imobilizata za uklanjanje 4-hlorfenola. Nakon osam ponovljenih ciklusa efikasnost Eupergit C250L-PFO imobilizata za uklanjanje 4-hlorfenola je pala na 55%.sr
dc.publisherSerbian Chemical Soc, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjectpolyphenol oxidaseen
dc.subjectpotatoen
dc.subjectpotatoen
dc.subjectphenolen
dc.subjectphenolen
dc.subjectimmobilizationen
dc.subjectimmobilizationen
dc.subjectEupergiten
dc.subjectEupergiten
dc.titleRemoval of aqueous phenol and phenol derivatives by immobilized potato polyphenol oxidaseen
dc.titleUklanjanje fenola i fenolnih derivata iz vode imobilizovanom polifenol-oksidazom iz krompirasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractAнделковиц, Иван; Дојнов, Биљана; Роглић, Горан; Вујциц, Мирослава; Бозиц, Натаса; Миловановиц, Aлександра; Лончар, Никола; Вујчић, Зоран; Уклањање фенола и фенолних деривата из воде имобилизованом полифенол-оксидазом из кромпира; Уклањање фенола и фенолних деривата из воде имобилизованом полифенол-оксидазом из кромпира;
dc.citation.volume76
dc.citation.issue4
dc.citation.spage513
dc.citation.epage522
dc.identifier.wos000290499600005
dc.identifier.doi10.2298/JSC100619046L
dc.citation.other76(4): 513-522
dc.citation.rankM23
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-79955003401
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/8495/1337.pdf
dc.identifier.rcubKon_2177


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