Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids
Nema prikaza
Autori
Sajewicz, MieczyslawStaszek, Dorota
Natić, Maja
Wojtal, Lukasz
Waksmundzka-Hajnos, Monika
Kowalska, Teresa
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separa...tions were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material.
Ključne reči:
flavonoids / mass spectrometric fingerprinting / phenolic acids / Salvia lavandulifolia / TLC-LC-MS / TLC-MSIzvor:
Journal of Liquid Chromatography and Related Technologies, 2011, 34, 10-11, 864-887Izdavač:
- Taylor & Francis Inc, Philadelphia
Finansiranje / projekti:
- Sinteza, analiza i aktivnost novih organskih polidentatnih liganada i njihovih kompleksa sa d-metalima (RS-MESTD-MPN2006-2010-142062)
- European Social Fund (EFS) of the European Union
DOI: 10.1080/10826076.2011.571131
ISSN: 1082-6076
WoS: 000290991600009
Scopus: 2-s2.0-79957500585
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Sajewicz, Mieczyslaw AU - Staszek, Dorota AU - Natić, Maja AU - Wojtal, Lukasz AU - Waksmundzka-Hajnos, Monika AU - Kowalska, Teresa PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1348 AB - In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separations were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Liquid Chromatography and Related Technologies T1 - Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids VL - 34 IS - 10-11 SP - 864 EP - 887 DO - 10.1080/10826076.2011.571131 ER -
@article{ author = "Sajewicz, Mieczyslaw and Staszek, Dorota and Natić, Maja and Wojtal, Lukasz and Waksmundzka-Hajnos, Monika and Kowalska, Teresa", year = "2011", abstract = "In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separations were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Liquid Chromatography and Related Technologies", title = "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids", volume = "34", number = "10-11", pages = "864-887", doi = "10.1080/10826076.2011.571131" }
Sajewicz, M., Staszek, D., Natić, M., Wojtal, L., Waksmundzka-Hajnos, M.,& Kowalska, T.. (2011). Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids. in Journal of Liquid Chromatography and Related Technologies Taylor & Francis Inc, Philadelphia., 34(10-11), 864-887. https://doi.org/10.1080/10826076.2011.571131
Sajewicz M, Staszek D, Natić M, Wojtal L, Waksmundzka-Hajnos M, Kowalska T. Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids. in Journal of Liquid Chromatography and Related Technologies. 2011;34(10-11):864-887. doi:10.1080/10826076.2011.571131 .
Sajewicz, Mieczyslaw, Staszek, Dorota, Natić, Maja, Wojtal, Lukasz, Waksmundzka-Hajnos, Monika, Kowalska, Teresa, "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids" in Journal of Liquid Chromatography and Related Technologies, 34, no. 10-11 (2011):864-887, https://doi.org/10.1080/10826076.2011.571131 . .