Aim of this work is to propose an analytical protocol for proteinaceous binder identification in paintings using tryptic peptide analysis and MALDI-TOF mass spectrometry strengthened with MALDI-TOF/TOF tandem mass spectrometry (LIFT method). Proteinaceous binders are enzymatically digested with trypsin. From each individual protein frequently occurring in binders, a specific set of peptides is releasing during enzymatic digestion giving a peptide mass fingerprint (PMF) of that particular protein. The most intensive peptide peaks in PMF were determined and annotated with their corresponding amino acid sequence by MALDI-TOF/TOF analysis and subsequent database search. Before analyzing historical painting samples, procedure was tested and optimized on several painting model samples for a reliable and efficient identification of proteinaceous materials. The method is avoiding sample manipulation as much as possible in order to reduce sample loss. Since the applied procedures led to protein... identification of binding media in model samples, MALDI-TOF/TOF was for the first time applied for analysis of proteinaceous binders in old painting samples.
Keywords:
MALDI TOF MS / LIFT-TOF/TOF tandem mass spectrometry / Peptide mass fingerprint / Proteinaceous binders / Historical paintings
TY - JOUR
AU - Tripković, T.
AU - Charvy, C.
AU - Alves, S.
AU - Lolić, Aleksandar
AU - Baošić, Rada
AU - Nikolić-Mandić, Snežana D.
AU - Tabet, J. C.
PY - 2013
UR - https://cherry.chem.bg.ac.rs/handle/123456789/1366
AB - Aim of this work is to propose an analytical protocol for proteinaceous binder identification in paintings using tryptic peptide analysis and MALDI-TOF mass spectrometry strengthened with MALDI-TOF/TOF tandem mass spectrometry (LIFT method). Proteinaceous binders are enzymatically digested with trypsin. From each individual protein frequently occurring in binders, a specific set of peptides is releasing during enzymatic digestion giving a peptide mass fingerprint (PMF) of that particular protein. The most intensive peptide peaks in PMF were determined and annotated with their corresponding amino acid sequence by MALDI-TOF/TOF analysis and subsequent database search. Before analyzing historical painting samples, procedure was tested and optimized on several painting model samples for a reliable and efficient identification of proteinaceous materials. The method is avoiding sample manipulation as much as possible in order to reduce sample loss. Since the applied procedures led to protein identification of binding media in model samples, MALDI-TOF/TOF was for the first time applied for analysis of proteinaceous binders in old painting samples.
PB - Elsevier Science Bv, Amsterdam
T2 - Talanta
T1 - Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry
VL - 113
SP - 49
EP - 61
DO - 10.1016/j.talanta.2013.03.071
UR - Kon_2486
ER -
@article{
author = "Tripković, T. and Charvy, C. and Alves, S. and Lolić, Aleksandar and Baošić, Rada and Nikolić-Mandić, Snežana D. and Tabet, J. C.",
year = "2013",
abstract = "Aim of this work is to propose an analytical protocol for proteinaceous binder identification in paintings using tryptic peptide analysis and MALDI-TOF mass spectrometry strengthened with MALDI-TOF/TOF tandem mass spectrometry (LIFT method). Proteinaceous binders are enzymatically digested with trypsin. From each individual protein frequently occurring in binders, a specific set of peptides is releasing during enzymatic digestion giving a peptide mass fingerprint (PMF) of that particular protein. The most intensive peptide peaks in PMF were determined and annotated with their corresponding amino acid sequence by MALDI-TOF/TOF analysis and subsequent database search. Before analyzing historical painting samples, procedure was tested and optimized on several painting model samples for a reliable and efficient identification of proteinaceous materials. The method is avoiding sample manipulation as much as possible in order to reduce sample loss. Since the applied procedures led to protein identification of binding media in model samples, MALDI-TOF/TOF was for the first time applied for analysis of proteinaceous binders in old painting samples.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Talanta",
title = "Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry",
volume = "113",
pages = "49-61",
doi = "10.1016/j.talanta.2013.03.071",
url = "Kon_2486"
}
Tripković, T., Charvy, C., Alves, S., Lolić, A., Baošić, R., Nikolić-Mandić, S. D.,& Tabet, J. C.. (2013). Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry. in Talanta
Elsevier Science Bv, Amsterdam., 113, 49-61.
https://doi.org/10.1016/j.talanta.2013.03.071
Kon_2486
Tripković T, Charvy C, Alves S, Lolić A, Baošić R, Nikolić-Mandić SD, Tabet JC. Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry. in Talanta. 2013;113:49-61.
doi:10.1016/j.talanta.2013.03.071
Kon_2486 .
Tripković, T., Charvy, C., Alves, S., Lolić, Aleksandar, Baošić, Rada, Nikolić-Mandić, Snežana D., Tabet, J. C., "Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry" in Talanta, 113 (2013):49-61,
https://doi.org/10.1016/j.talanta.2013.03.071 .,
Kon_2486 .
