Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes
Само за регистроване кориснике
2013
Аутори
Vlajkovic, JelenaAndrić, Filip
Ristivojević, Petar
Radoičić, Aleksandra
Tešić, Živoslav Lj.
Milojković-Opsenica, Dušanka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A novel reversed-phase thin-layer chromatographic (RP-TLC) method was developed for simultaneous determination of ten most commonly used artificial food dyes. The method was validated with respect to specificity, linearity, precision, and accuracy and compared with reversed-phase high performance liquid chromatography (RP-HPLC) method. In the case of TLC method horizontal developing technique was employed using octadecyl-modified silica as a stationary phase and 0.5mol dm(-3) ammonium sulfate in 30% of ethanol-water solution as a mobile phase. HPLC analysis was carried out using XDB-C18 column and gradient of mixture of aqueous ammonium acetate solution (0.1moldm(-3), pH=6.5) and MeOH/ACN (1:1, v/v). Colorants were detected at 450, 500, and 625nm wavelengths. The TLC method proved to be suitable for quantitative determination in relatively wide range of concentrations (20-300ng/band). Satisfactory values of limits of detection and quantitation were established for both techniques, alth...ough somewhat higher in the case of TLC, ranging from 2-10ng/band, that is, 3-20ng/band. Both methods can be applied with satisfactory precision and accuracy, with clear advantage of TLC for simultaneous analysis of six samples per hour.
Кључне речи:
Food quality control / green analytical chemistry / method development / reversed-phase high-performance liquid chromatography / reversed-phase thin-layer chromatography / synthetic food colorantsИзвор:
Journal of Liquid Chromatography and Related Technologies, 2013, 36, 17, 2476-2488Издавач:
- Taylor & Francis Inc, Philadelphia
Финансирање / пројекти:
- Корелација структуре и особина природних и синтетичких молекула и њихових комплекса са металима (RS-172017)
DOI: 10.1080/10826076.2013.790771
ISSN: 1082-6076
WoS: 000321812900012
Scopus: 2-s2.0-84880338169
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Vlajkovic, Jelena AU - Andrić, Filip AU - Ristivojević, Petar AU - Radoičić, Aleksandra AU - Tešić, Živoslav Lj. AU - Milojković-Opsenica, Dušanka PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1374 AB - A novel reversed-phase thin-layer chromatographic (RP-TLC) method was developed for simultaneous determination of ten most commonly used artificial food dyes. The method was validated with respect to specificity, linearity, precision, and accuracy and compared with reversed-phase high performance liquid chromatography (RP-HPLC) method. In the case of TLC method horizontal developing technique was employed using octadecyl-modified silica as a stationary phase and 0.5mol dm(-3) ammonium sulfate in 30% of ethanol-water solution as a mobile phase. HPLC analysis was carried out using XDB-C18 column and gradient of mixture of aqueous ammonium acetate solution (0.1moldm(-3), pH=6.5) and MeOH/ACN (1:1, v/v). Colorants were detected at 450, 500, and 625nm wavelengths. The TLC method proved to be suitable for quantitative determination in relatively wide range of concentrations (20-300ng/band). Satisfactory values of limits of detection and quantitation were established for both techniques, although somewhat higher in the case of TLC, ranging from 2-10ng/band, that is, 3-20ng/band. Both methods can be applied with satisfactory precision and accuracy, with clear advantage of TLC for simultaneous analysis of six samples per hour. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Liquid Chromatography and Related Technologies T1 - Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes VL - 36 IS - 17 SP - 2476 EP - 2488 DO - 10.1080/10826076.2013.790771 ER -
@article{ author = "Vlajkovic, Jelena and Andrić, Filip and Ristivojević, Petar and Radoičić, Aleksandra and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka", year = "2013", abstract = "A novel reversed-phase thin-layer chromatographic (RP-TLC) method was developed for simultaneous determination of ten most commonly used artificial food dyes. The method was validated with respect to specificity, linearity, precision, and accuracy and compared with reversed-phase high performance liquid chromatography (RP-HPLC) method. In the case of TLC method horizontal developing technique was employed using octadecyl-modified silica as a stationary phase and 0.5mol dm(-3) ammonium sulfate in 30% of ethanol-water solution as a mobile phase. HPLC analysis was carried out using XDB-C18 column and gradient of mixture of aqueous ammonium acetate solution (0.1moldm(-3), pH=6.5) and MeOH/ACN (1:1, v/v). Colorants were detected at 450, 500, and 625nm wavelengths. The TLC method proved to be suitable for quantitative determination in relatively wide range of concentrations (20-300ng/band). Satisfactory values of limits of detection and quantitation were established for both techniques, although somewhat higher in the case of TLC, ranging from 2-10ng/band, that is, 3-20ng/band. Both methods can be applied with satisfactory precision and accuracy, with clear advantage of TLC for simultaneous analysis of six samples per hour.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Liquid Chromatography and Related Technologies", title = "Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes", volume = "36", number = "17", pages = "2476-2488", doi = "10.1080/10826076.2013.790771" }
Vlajkovic, J., Andrić, F., Ristivojević, P., Radoičić, A., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2013). Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes. in Journal of Liquid Chromatography and Related Technologies Taylor & Francis Inc, Philadelphia., 36(17), 2476-2488. https://doi.org/10.1080/10826076.2013.790771
Vlajkovic J, Andrić F, Ristivojević P, Radoičić A, Tešić ŽL, Milojković-Opsenica D. Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes. in Journal of Liquid Chromatography and Related Technologies. 2013;36(17):2476-2488. doi:10.1080/10826076.2013.790771 .
Vlajkovic, Jelena, Andrić, Filip, Ristivojević, Petar, Radoičić, Aleksandra, Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes" in Journal of Liquid Chromatography and Related Technologies, 36, no. 17 (2013):2476-2488, https://doi.org/10.1080/10826076.2013.790771 . .