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dc.creatorLončar, Nikola L.
dc.creatorBožić, Nataša
dc.creatorLopez-Santin, Josep
dc.creatorVujčić, Zoran
dc.date.accessioned2018-11-22T00:24:27Z
dc.date.available2018-11-22T00:24:27Z
dc.date.issued2013
dc.identifier.issn0960-8524
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1431
dc.description.abstractOne hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 degrees C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 degrees C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color. (C) 2013 Elsevier Ltd. All rights reserved.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationJoint Serbian-Spanish Action
dc.relationSpanish MICINN [CTQ2011-28398-CO2-01]
dc.rightsrestrictedAccess
dc.sourceBioresource Technology
dc.subjectLaccaseen
dc.subjectBacillus amyloliquefaciensen
dc.subjectDecolorizationen
dc.subjectBiodegradationen
dc.titleBacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorizationen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујчић, Зоран; Лончар, Никола; Лопез-Сантин, Јосеп; Бозиц, Натаса;
dc.citation.volume147
dc.citation.spage177
dc.citation.epage183
dc.identifier.wos000326660900026
dc.identifier.doi10.1016/j.biortech.2013.08.056
dc.citation.other147: 177-183
dc.citation.rankaM21
dc.identifier.pmid23994699
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84883380664
dc.identifier.rcubKon_2551


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