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Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity

Authorized Users Only
2013
Authors
Stanković, Nada
Šenerović, Lidija
Bojić-Trbojević, Žanka
Vučković, Ivan
Vicovac, Ljiljana
Vasiljević, Branka
Nikodinović-Runić, Jasmina
Article (Published version)
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Abstract
AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less t...oxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.

Keywords:
actinomycetes / antimicrobials / cytotoxicity / fungi / optimization
Source:
Journal of Applied Microbiology, 2013, 115, 6, 1297-1306
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Microbial diversity study and characterization of beneficial environmental microorganisms (RS-173048)
  • Trophoblast and extraembryonic fetal cells: plasticity, differentiation factors and in vitro modulation of functional characteristics (RS-173004)
Note:
  • Free full text: https://doi.org/10.1111/jam.12326
  • Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/3486
  • Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3487

DOI: 10.1111/jam.12326

ISSN: 1364-5072

PubMed: 23941529

WoS: 000326939800005

Scopus: 2-s2.0-84887823790
[ Google Scholar ]
9
8
URI
https://cherry.chem.bg.ac.rs/handle/123456789/1438
Collections
  • Publikacije
Institution/Community
Inovacioni centar
TY  - JOUR
AU  - Stanković, Nada
AU  - Šenerović, Lidija
AU  - Bojić-Trbojević, Žanka
AU  - Vučković, Ivan
AU  - Vicovac, Ljiljana
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1438
AB  - AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity
VL  - 115
IS  - 6
SP  - 1297
EP  - 1306
DO  - 10.1111/jam.12326
UR  - Kon_2558
ER  - 
@article{
author = "Stanković, Nada and Šenerović, Lidija and Bojić-Trbojević, Žanka and Vučković, Ivan and Vicovac, Ljiljana and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2013",
abstract = "AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity",
volume = "115",
number = "6",
pages = "1297-1306",
doi = "10.1111/jam.12326",
url = "Kon_2558"
}
Stanković, N., Šenerović, L., Bojić-Trbojević, Ž., Vučković, I., Vicovac, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology
Wiley, Hoboken., 115(6), 1297-1306.
https://doi.org/10.1111/jam.12326
Kon_2558
Stanković N, Šenerović L, Bojić-Trbojević Ž, Vučković I, Vicovac L, Vasiljević B, Nikodinović-Runić J. Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology. 2013;115(6):1297-1306.
doi:10.1111/jam.12326
Kon_2558 .
Stanković, Nada, Šenerović, Lidija, Bojić-Trbojević, Žanka, Vučković, Ivan, Vicovac, Ljiljana, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity" in Journal of Applied Microbiology, 115, no. 6 (2013):1297-1306,
https://doi.org/10.1111/jam.12326 .,
Kon_2558 .

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