Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis
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Gašić, Uroš M.
Tešić, Živoslav Lj.
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IntroductionPropolis is a resinous natural substance collected by honeybees from different plant sources. Due to the presence of various phytochemicals, this bee-product exhibits numerous biological activities, including anti-bacterial, anti-viral, anti-inflammatory, anti-oxidant, immunostimulating and anti-tumour effects. As the chemical composition and biological activity of propolis depend on its botanical and geographical origin, searching for new bioactive substances in various types of propolis from unexplored regions is of great importance. ObjectiveThe aim of this study is the evaluation of the phenolic profile of poplar propolis samples in order to characterise Serbian propolis, to identify possible new constituents and to specify the phenolic components relevant for differentiation of poplar propolis samples into two subgroups through simultaneous analysis of poplar bud extracts. MethodsEthanolic extracts of propolis and poplar buds were comprehensively analysed using ultrahi...gh-performance liquid chromatography coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser together with chemometric methods. ResultsExtensive fingerprint analysis of Serbian propolis was achieved for the first time. Seventy-five phenolic compounds were detected. Eight of them were identified in propolis for the first time. Pattern-recognition methods applied to the content of ten quantified phenolics verified the existence of two subgroups of propolis, with galangin, chrysin and pinocembrin as the most influential distinguishing factors. ConclusionThe phenolic composition of the analysed propolis samples confirm their affiliation to the European poplar type propolis and the existence of two subgroups according to botanical origin. Copyright (c) 2014 John Wiley & Sons, Ltd. The phenolic profiles of Serbian propolis samples were determined using ultrahigh-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser. Fingerprint analysis revealed the existence of 75 phenolic compounds. Eight of them were identified in propolis for the first time. The chemical composition of the samples analysed confirmed their affiliation to the European poplar propolis and the existence of two subgroups according to their phenolic profile.
Keywords:Pattern-recognition methods / phenolic profile / UHPLC-LTQ / Orbitrap / MS / MS / poplar type propolis
Source:Phytochemical Analysis, 2015, 26, 2, 127-136
- Wiley-Blackwell, Hoboken
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-172017)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3458