Faculty of Chemistry Repository - Cherry
University of Belgrade - Faculty of Chemistry
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   Faculty of Chemistry Repository - Cherry
  • Inovacioni centar
  • Publikacije
  • View Item
  •   Faculty of Chemistry Repository - Cherry
  • Inovacioni centar
  • Publikacije
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Limited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolates

Thumbnail
2013
1491.pdf (969.6Kb)
Authors
Minovska, Gordana
Narančić, Tanja
Mandić, Mina
Šenerović, Lidija
Vasiljević, Branka
Nikodinović-Runić, Jasmina
Article (Published version)
Metadata
Show full item record
Abstract
Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterised corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Baci...llus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.

Keywords:
Biodegradation / aromatic compounds / 4-oxalocrotonate tautomerase
Source:
Genetika (Beograd), 2013, 45, 3, 703-716
Publisher:
  • Serbian Genetics Soc, Belgrade
Projects:
  • Microbial diversity study and characterization of beneficial environmental microorganisms (RS-173048)

DOI: 10.2298/GENSR1303703M

ISSN: 0534-0012

WoS: 000330186600008

Scopus: 2-s2.0-84894584979
[ Google Scholar ]
1
1
URI
http://cherry.chem.bg.ac.rs/handle/123456789/1493
Collections
  • Publikacije
Institution
Inovacioni centar

DSpace software copyright © 2002-2015  DuraSpace
About CHERRY - CHEmistry RepositoRY | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CHERRY - CHEmistry RepositoRY | Send Feedback

OpenAIRERCUB