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dc.creatorMinovska, Gordana
dc.creatorNarančić, Tanja
dc.creatorMandić, Mina
dc.creatorŠenerović, Lidija
dc.creatorVasiljević, Branka
dc.creatorNikodinović-Runić, Jasmina
dc.date.accessioned2018-11-22T00:25:00Z
dc.date.available2018-11-22T00:25:00Z
dc.date.issued2013
dc.identifier.issn0534-0012
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1493
dc.description.abstractIdentification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterised corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.en
dc.publisherSerbian Genetics Soc, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173048/RS//
dc.rightsopenAccess
dc.sourceGenetika (Beograd)
dc.subjectBiodegradationen
dc.subjectaromatic compoundsen
dc.subject4-oxalocrotonate tautomeraseen
dc.titleLimited Aromatic Pathway Genes Diversity Amongst Aromatic Compound Degrading Soil Bacterial Isolatesen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractМандиц, Мина; Васиљевиц, Бранка; Сенеровиц, Лидија; Наранциц, Тања; Никодиновић-Рунић, Јасмина; Миновска, Гордана;
dc.citation.volume45
dc.citation.issue3
dc.citation.spage703
dc.citation.epage716
dc.identifier.wos000330186600008
dc.identifier.doi10.2298/GENSR1303703M
dc.citation.other45(3): 703-716
dc.citation.rankM23
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-84894584979
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/8606/1491.pdf
dc.identifier.rcubKon_2613


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