Microbial Diversity and Isolation of Multiple Metal-Tolerant Bacteria from Surface and Underground Pits Within the Copper Mining and Smelting Complex Bor
2013
Authors
Bajkic, SanjaNarančić, Tanja
Đokić, Lidija
Đorđević, Dragana S.
Nikodinović-Runić, Jasmina
Morić, Ivana
Vasiljević, Branka
Article (Published version)
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The bacterial diversity of the surface and deep sediment of the Copper Mining and Smelting Complex Bor, Serbia, was investigated using culture-dependent and culture-independent approaches. Sequencing analysis of 16S rDNA libraries revealed greater bacterial diversity in the surface sediment of the mining complex (MS) in comparison to deeper mine sediment (MU). While in the MS sample members of seven different phylogenetic groups were detected, in the MU sample library representatives of only three different groups were detected. The use of a culture-dependent approach revealed the presence of only three bacterial groups in both samples: Actinobacteria, Firmicutes and Proteobacteria, while six isolates exhibiting the highest metal tolerance were members of Arthrobacter and Staphylococcus genera. The most promising isolate, MSI08, was able to grow in the presence of high concentrations of Cd2+ (535 mu M), Ni2+(17 mM) and Cr6+ (38.5 mM) and as such this indigenous strain has potential in ...the bioremediation of the contaminated surrounds of the city of Bor.
Keywords:
Copper mine / bacterial diversity / heavy metal tolerance / StaphylococcusSource:
Archives of biological sciences, 2013, 65, 1, 375-386Publisher:
- Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Funding / projects:
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.2298/ABS1301375B
ISSN: 0354-4664
WoS: 000312046300044
Scopus: 2-s2.0-84873253250
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Inovacioni centar / Innovation CentreTY - JOUR AU - Bajkic, Sanja AU - Narančić, Tanja AU - Đokić, Lidija AU - Đorđević, Dragana S. AU - Nikodinović-Runić, Jasmina AU - Morić, Ivana AU - Vasiljević, Branka PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1559 AB - The bacterial diversity of the surface and deep sediment of the Copper Mining and Smelting Complex Bor, Serbia, was investigated using culture-dependent and culture-independent approaches. Sequencing analysis of 16S rDNA libraries revealed greater bacterial diversity in the surface sediment of the mining complex (MS) in comparison to deeper mine sediment (MU). While in the MS sample members of seven different phylogenetic groups were detected, in the MU sample library representatives of only three different groups were detected. The use of a culture-dependent approach revealed the presence of only three bacterial groups in both samples: Actinobacteria, Firmicutes and Proteobacteria, while six isolates exhibiting the highest metal tolerance were members of Arthrobacter and Staphylococcus genera. The most promising isolate, MSI08, was able to grow in the presence of high concentrations of Cd2+ (535 mu M), Ni2+(17 mM) and Cr6+ (38.5 mM) and as such this indigenous strain has potential in the bioremediation of the contaminated surrounds of the city of Bor. PB - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd T2 - Archives of biological sciences T1 - Microbial Diversity and Isolation of Multiple Metal-Tolerant Bacteria from Surface and Underground Pits Within the Copper Mining and Smelting Complex Bor VL - 65 IS - 1 SP - 375 EP - 386 DO - 10.2298/ABS1301375B ER -
@article{ author = "Bajkic, Sanja and Narančić, Tanja and Đokić, Lidija and Đorđević, Dragana S. and Nikodinović-Runić, Jasmina and Morić, Ivana and Vasiljević, Branka", year = "2013", abstract = "The bacterial diversity of the surface and deep sediment of the Copper Mining and Smelting Complex Bor, Serbia, was investigated using culture-dependent and culture-independent approaches. Sequencing analysis of 16S rDNA libraries revealed greater bacterial diversity in the surface sediment of the mining complex (MS) in comparison to deeper mine sediment (MU). While in the MS sample members of seven different phylogenetic groups were detected, in the MU sample library representatives of only three different groups were detected. The use of a culture-dependent approach revealed the presence of only three bacterial groups in both samples: Actinobacteria, Firmicutes and Proteobacteria, while six isolates exhibiting the highest metal tolerance were members of Arthrobacter and Staphylococcus genera. The most promising isolate, MSI08, was able to grow in the presence of high concentrations of Cd2+ (535 mu M), Ni2+(17 mM) and Cr6+ (38.5 mM) and as such this indigenous strain has potential in the bioremediation of the contaminated surrounds of the city of Bor.", publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd", journal = "Archives of biological sciences", title = "Microbial Diversity and Isolation of Multiple Metal-Tolerant Bacteria from Surface and Underground Pits Within the Copper Mining and Smelting Complex Bor", volume = "65", number = "1", pages = "375-386", doi = "10.2298/ABS1301375B" }
Bajkic, S., Narančić, T., Đokić, L., Đorđević, D. S., Nikodinović-Runić, J., Morić, I.,& Vasiljević, B.. (2013). Microbial Diversity and Isolation of Multiple Metal-Tolerant Bacteria from Surface and Underground Pits Within the Copper Mining and Smelting Complex Bor. in Archives of biological sciences Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 65(1), 375-386. https://doi.org/10.2298/ABS1301375B
Bajkic S, Narančić T, Đokić L, Đorđević DS, Nikodinović-Runić J, Morić I, Vasiljević B. Microbial Diversity and Isolation of Multiple Metal-Tolerant Bacteria from Surface and Underground Pits Within the Copper Mining and Smelting Complex Bor. in Archives of biological sciences. 2013;65(1):375-386. doi:10.2298/ABS1301375B .
Bajkic, Sanja, Narančić, Tanja, Đokić, Lidija, Đorđević, Dragana S., Nikodinović-Runić, Jasmina, Morić, Ivana, Vasiljević, Branka, "Microbial Diversity and Isolation of Multiple Metal-Tolerant Bacteria from Surface and Underground Pits Within the Copper Mining and Smelting Complex Bor" in Archives of biological sciences, 65, no. 1 (2013):375-386, https://doi.org/10.2298/ABS1301375B . .