Monitoring of the human serum albumin carbonylation level through determination of guanidino group content
Само за регистроване кориснике
2013
Аутори
Aćimović, Jelena M.Jovanović, Vesna B.
Sreckovic, Vesna Dimitrijevic
Penezić-Romanjuk, Ana Z.
Mandić, Ljuba M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Carbonylation of the protein amino, guanidine, and thiol groups with alpha-oxoaldehydes (which are produced in higher quantities in diabetes, uremia, oxidative stress, aging, and inflammation) is one of the important causes of vascular complications. For monitoring of the human serum albumin (HSA) carbonylation level, a spectrophotometric method based on the formation of colored adduct between guanidine group and thymol-sodium hypobromite reagent in the alkaline medium was investigated. Beer's law is obeyed in the concentration range of Arg and protein guanidine groups from 1 to 40 mM. Precision of the method (relative standard deviation) was in the range of 0.9 to 2%. Accuracy was examined by the standard addition method (recovery similar to 100%). The method was applied for monitoring of the carbonylation level of HSA with methylglyoxal in vitro and of HSA isolated (using affinity chromatography) from sera of 21 patients with type 2 diabetes and 12 healthy persons. The content of gua...nidine groups in HSA isolated from diabetics (19.64 +/- 1.07 mM/mM albumin) was significantly lower (P lt 0.001) in comparison with a control group (21.87 +/- 1.02 mM/mM albumin). The method is simple and fast, has good accuracy and precision, and is suitable for clinical practice as well for in vitro protein carbonylation experiments.
Кључне речи:
Carbonylation of HSA guanidine group / Methylglyoxal / Diabetes / Spectrophotometric determinationИзвор:
Analytical Biochemistry, 2013, 433, 2, 162-167Издавач:
- Academic Press Inc Elsevier Science, San Diego
Финансирање / пројекти:
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1016/j.ab.2012.10.028
ISSN: 0003-2697
PubMed: 23103397
WoS: 000314009300014
Scopus: 2-s2.0-84870690241
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Aćimović, Jelena M. AU - Jovanović, Vesna B. AU - Sreckovic, Vesna Dimitrijevic AU - Penezić-Romanjuk, Ana Z. AU - Mandić, Ljuba M. PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1578 AB - Carbonylation of the protein amino, guanidine, and thiol groups with alpha-oxoaldehydes (which are produced in higher quantities in diabetes, uremia, oxidative stress, aging, and inflammation) is one of the important causes of vascular complications. For monitoring of the human serum albumin (HSA) carbonylation level, a spectrophotometric method based on the formation of colored adduct between guanidine group and thymol-sodium hypobromite reagent in the alkaline medium was investigated. Beer's law is obeyed in the concentration range of Arg and protein guanidine groups from 1 to 40 mM. Precision of the method (relative standard deviation) was in the range of 0.9 to 2%. Accuracy was examined by the standard addition method (recovery similar to 100%). The method was applied for monitoring of the carbonylation level of HSA with methylglyoxal in vitro and of HSA isolated (using affinity chromatography) from sera of 21 patients with type 2 diabetes and 12 healthy persons. The content of guanidine groups in HSA isolated from diabetics (19.64 +/- 1.07 mM/mM albumin) was significantly lower (P lt 0.001) in comparison with a control group (21.87 +/- 1.02 mM/mM albumin). The method is simple and fast, has good accuracy and precision, and is suitable for clinical practice as well for in vitro protein carbonylation experiments. PB - Academic Press Inc Elsevier Science, San Diego T2 - Analytical Biochemistry T1 - Monitoring of the human serum albumin carbonylation level through determination of guanidino group content VL - 433 IS - 2 SP - 162 EP - 167 DO - 10.1016/j.ab.2012.10.028 ER -
@article{ author = "Aćimović, Jelena M. and Jovanović, Vesna B. and Sreckovic, Vesna Dimitrijevic and Penezić-Romanjuk, Ana Z. and Mandić, Ljuba M.", year = "2013", abstract = "Carbonylation of the protein amino, guanidine, and thiol groups with alpha-oxoaldehydes (which are produced in higher quantities in diabetes, uremia, oxidative stress, aging, and inflammation) is one of the important causes of vascular complications. For monitoring of the human serum albumin (HSA) carbonylation level, a spectrophotometric method based on the formation of colored adduct between guanidine group and thymol-sodium hypobromite reagent in the alkaline medium was investigated. Beer's law is obeyed in the concentration range of Arg and protein guanidine groups from 1 to 40 mM. Precision of the method (relative standard deviation) was in the range of 0.9 to 2%. Accuracy was examined by the standard addition method (recovery similar to 100%). The method was applied for monitoring of the carbonylation level of HSA with methylglyoxal in vitro and of HSA isolated (using affinity chromatography) from sera of 21 patients with type 2 diabetes and 12 healthy persons. The content of guanidine groups in HSA isolated from diabetics (19.64 +/- 1.07 mM/mM albumin) was significantly lower (P lt 0.001) in comparison with a control group (21.87 +/- 1.02 mM/mM albumin). The method is simple and fast, has good accuracy and precision, and is suitable for clinical practice as well for in vitro protein carbonylation experiments.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Analytical Biochemistry", title = "Monitoring of the human serum albumin carbonylation level through determination of guanidino group content", volume = "433", number = "2", pages = "162-167", doi = "10.1016/j.ab.2012.10.028" }
Aćimović, J. M., Jovanović, V. B., Sreckovic, V. D., Penezić-Romanjuk, A. Z.,& Mandić, L. M.. (2013). Monitoring of the human serum albumin carbonylation level through determination of guanidino group content. in Analytical Biochemistry Academic Press Inc Elsevier Science, San Diego., 433(2), 162-167. https://doi.org/10.1016/j.ab.2012.10.028
Aćimović JM, Jovanović VB, Sreckovic VD, Penezić-Romanjuk AZ, Mandić LM. Monitoring of the human serum albumin carbonylation level through determination of guanidino group content. in Analytical Biochemistry. 2013;433(2):162-167. doi:10.1016/j.ab.2012.10.028 .
Aćimović, Jelena M., Jovanović, Vesna B., Sreckovic, Vesna Dimitrijevic, Penezić-Romanjuk, Ana Z., Mandić, Ljuba M., "Monitoring of the human serum albumin carbonylation level through determination of guanidino group content" in Analytical Biochemistry, 433, no. 2 (2013):162-167, https://doi.org/10.1016/j.ab.2012.10.028 . .