Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study)
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2013
Authors
Miljević, ČedoNikolić-Kokić, Aleksandra
Nikolić, Milan
Niketić, Vesna
Spasić, Mihajlo B.
Lecic-Tosevski, Dusica
Blagojević, Duško P.
Article (Published version)
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Objective This study was set out to examine the impact of atypical antipsychotic drugs: aripiprazole, clozapine, ziprasidone, olanzapine, quetiapine, sertindole and amisulpride on the activity of antioxidant defence enzymes in human erythrocytes in vitro. Methods Cu,Zn-superoxide dismutase (SOD1), catalase (CAT), selenium-dependent glutathione peroxidase and glutathione reductase activities were determined after drugs incubation with blood of 15 apparently healthy non-smoking male volunteers (ages 2339) for 1?h at 37?degrees C. Results A statistically significant increase in SOD1 activity was found in samples incubated with aripiprazole (p? lt ?0.01) and quetiapine (p? lt ?0.05) compared with incubated control. SOD1 activity profile following native polyacrylamide gel electrophoresis indicates that aripiprazole and quetiapine protect enzyme activity from inhibition with hydrogen peroxide. Our results showed that sertindole decreases activity of CAT comparing with control non-treated er...ythrocytes. Moreover, in sertindole treated erythrocytes, negative correlation between SOD1 and glutathione peroxidase activities was found. Increased amount of hydrogen peroxide in such situation may leave erythrocytes and transform their role in circulation from anti-oxidative to pro-oxidative. Conclusions Our results indicate that mechanism through sertindole could express its in vivo toxic effects and point toward possible (neuro)protective effects of aripiprazole and quetiapine.
Keywords:
atypical antipsychotics / oxidative stress / anti-oxidant defence enzymesSource:
Human Psychopharmacology: Clinical and Experimental, 2013, 28, 1, 1-6Publisher:
- Wiley, Hoboken
Funding / projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-MESTD-Basic Research (BR or ON)-173014)
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-MESTD-Basic Research (BR or ON)-172035)
DOI: 10.1002/hup.2272
ISSN: 0885-6222
PubMed: 23124725
WoS: 000314184900002
Scopus: 2-s2.0-84873869323
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Miljević, Čedo AU - Nikolić-Kokić, Aleksandra AU - Nikolić, Milan AU - Niketić, Vesna AU - Spasić, Mihajlo B. AU - Lecic-Tosevski, Dusica AU - Blagojević, Duško P. PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1581 AB - Objective This study was set out to examine the impact of atypical antipsychotic drugs: aripiprazole, clozapine, ziprasidone, olanzapine, quetiapine, sertindole and amisulpride on the activity of antioxidant defence enzymes in human erythrocytes in vitro. Methods Cu,Zn-superoxide dismutase (SOD1), catalase (CAT), selenium-dependent glutathione peroxidase and glutathione reductase activities were determined after drugs incubation with blood of 15 apparently healthy non-smoking male volunteers (ages 2339) for 1?h at 37?degrees C. Results A statistically significant increase in SOD1 activity was found in samples incubated with aripiprazole (p? lt ?0.01) and quetiapine (p? lt ?0.05) compared with incubated control. SOD1 activity profile following native polyacrylamide gel electrophoresis indicates that aripiprazole and quetiapine protect enzyme activity from inhibition with hydrogen peroxide. Our results showed that sertindole decreases activity of CAT comparing with control non-treated erythrocytes. Moreover, in sertindole treated erythrocytes, negative correlation between SOD1 and glutathione peroxidase activities was found. Increased amount of hydrogen peroxide in such situation may leave erythrocytes and transform their role in circulation from anti-oxidative to pro-oxidative. Conclusions Our results indicate that mechanism through sertindole could express its in vivo toxic effects and point toward possible (neuro)protective effects of aripiprazole and quetiapine. PB - Wiley, Hoboken T2 - Human Psychopharmacology: Clinical and Experimental T1 - Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study) VL - 28 IS - 1 SP - 1 EP - 6 DO - 10.1002/hup.2272 ER -
@article{ author = "Miljević, Čedo and Nikolić-Kokić, Aleksandra and Nikolić, Milan and Niketić, Vesna and Spasić, Mihajlo B. and Lecic-Tosevski, Dusica and Blagojević, Duško P.", year = "2013", abstract = "Objective This study was set out to examine the impact of atypical antipsychotic drugs: aripiprazole, clozapine, ziprasidone, olanzapine, quetiapine, sertindole and amisulpride on the activity of antioxidant defence enzymes in human erythrocytes in vitro. Methods Cu,Zn-superoxide dismutase (SOD1), catalase (CAT), selenium-dependent glutathione peroxidase and glutathione reductase activities were determined after drugs incubation with blood of 15 apparently healthy non-smoking male volunteers (ages 2339) for 1?h at 37?degrees C. Results A statistically significant increase in SOD1 activity was found in samples incubated with aripiprazole (p? lt ?0.01) and quetiapine (p? lt ?0.05) compared with incubated control. SOD1 activity profile following native polyacrylamide gel electrophoresis indicates that aripiprazole and quetiapine protect enzyme activity from inhibition with hydrogen peroxide. Our results showed that sertindole decreases activity of CAT comparing with control non-treated erythrocytes. Moreover, in sertindole treated erythrocytes, negative correlation between SOD1 and glutathione peroxidase activities was found. Increased amount of hydrogen peroxide in such situation may leave erythrocytes and transform their role in circulation from anti-oxidative to pro-oxidative. Conclusions Our results indicate that mechanism through sertindole could express its in vivo toxic effects and point toward possible (neuro)protective effects of aripiprazole and quetiapine.", publisher = "Wiley, Hoboken", journal = "Human Psychopharmacology: Clinical and Experimental", title = "Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study)", volume = "28", number = "1", pages = "1-6", doi = "10.1002/hup.2272" }
Miljević, Č., Nikolić-Kokić, A., Nikolić, M., Niketić, V., Spasić, M. B., Lecic-Tosevski, D.,& Blagojević, D. P.. (2013). Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study). in Human Psychopharmacology: Clinical and Experimental Wiley, Hoboken., 28(1), 1-6. https://doi.org/10.1002/hup.2272
Miljević Č, Nikolić-Kokić A, Nikolić M, Niketić V, Spasić MB, Lecic-Tosevski D, Blagojević DP. Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study). in Human Psychopharmacology: Clinical and Experimental. 2013;28(1):1-6. doi:10.1002/hup.2272 .
Miljević, Čedo, Nikolić-Kokić, Aleksandra, Nikolić, Milan, Niketić, Vesna, Spasić, Mihajlo B., Lecic-Tosevski, Dusica, Blagojević, Duško P., "Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study)" in Human Psychopharmacology: Clinical and Experimental, 28, no. 1 (2013):1-6, https://doi.org/10.1002/hup.2272 . .