The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry
Само за регистроване кориснике
2013
Аутори
Keckes, SilvioGašić, Uroš M.
Ćirković-Veličković, Tanja
Milojković-Opsenica, Dušanka
Natić, Maja
Tešić, Živoslav Lj.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyphenolic profiles of 44 unifloral Serbian honeys were analyzed using ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. Rapid UHPLC method was developed in combination with a high sensitivity accurate mass scan and a simultaneous data dependent scan. The honey samples were of different botanical origin: acacia (Robinia pseudoacacia), sunflower (Helianthus annuus), linden (Tilia cordata), basil (Ocimum basilicum), buckwheat (Fagopyrum esculentum), oilseed rape (Brassica napus), and goldenrod (Solidago virgaurea). The presence of 43 compounds, mainly flavonoids, was proven in all honey samples by their characteristic mass spectra and fragmentation pattern. Relatively high amounts of chrysin, pinocembrin and galangin were identified in all honey extracts. p-Coumaric acid was not detected in basil, buckwheat and goldenrod honey extracts. A larger amount of gallic acid (ma...x value 1.45 mg/kg) was found in the sunflower honey, while a larger amount of apigenin (0.97 mg/kg) was determined in the buckwheat honey in comparison with other honeys. The samples were classified according to the botanical origin using pattern recognition technique, Principal Component Analysis (PCA). The LTQ OrbiTrap technique was proven to be reliable for the unambiguous detection of phenolic acids, their derivatives, and flavonoid aglycones based on their molecular masses and fragmentation pattern.
Кључне речи:
Serbian honey / Polyphenols / UHPLC-LTQ OrbiTrap MS / Botanical originИзвор:
Food Chemistry, 2013, 138, 1, 32-40Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Корелација структуре и особина природних и синтетичких молекула и њихових комплекса са металима (RS-MESTD-Basic Research (BR or ON)-172017)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-FP7-256716)
Напомена:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3552
DOI: 10.1016/j.foodchem.2012.10.025
ISSN: 0308-8146
PubMed: 23265452
WoS: 000314193800006
Scopus: 2-s2.0-84869234018
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Keckes, Silvio AU - Gašić, Uroš M. AU - Ćirković-Veličković, Tanja AU - Milojković-Opsenica, Dušanka AU - Natić, Maja AU - Tešić, Živoslav Lj. PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1582 AB - Polyphenolic profiles of 44 unifloral Serbian honeys were analyzed using ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. Rapid UHPLC method was developed in combination with a high sensitivity accurate mass scan and a simultaneous data dependent scan. The honey samples were of different botanical origin: acacia (Robinia pseudoacacia), sunflower (Helianthus annuus), linden (Tilia cordata), basil (Ocimum basilicum), buckwheat (Fagopyrum esculentum), oilseed rape (Brassica napus), and goldenrod (Solidago virgaurea). The presence of 43 compounds, mainly flavonoids, was proven in all honey samples by their characteristic mass spectra and fragmentation pattern. Relatively high amounts of chrysin, pinocembrin and galangin were identified in all honey extracts. p-Coumaric acid was not detected in basil, buckwheat and goldenrod honey extracts. A larger amount of gallic acid (max value 1.45 mg/kg) was found in the sunflower honey, while a larger amount of apigenin (0.97 mg/kg) was determined in the buckwheat honey in comparison with other honeys. The samples were classified according to the botanical origin using pattern recognition technique, Principal Component Analysis (PCA). The LTQ OrbiTrap technique was proven to be reliable for the unambiguous detection of phenolic acids, their derivatives, and flavonoid aglycones based on their molecular masses and fragmentation pattern. PB - Elsevier Sci Ltd, Oxford T2 - Food Chemistry T1 - The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry VL - 138 IS - 1 SP - 32 EP - 40 DO - 10.1016/j.foodchem.2012.10.025 ER -
@article{ author = "Keckes, Silvio and Gašić, Uroš M. and Ćirković-Veličković, Tanja and Milojković-Opsenica, Dušanka and Natić, Maja and Tešić, Živoslav Lj.", year = "2013", abstract = "Polyphenolic profiles of 44 unifloral Serbian honeys were analyzed using ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. Rapid UHPLC method was developed in combination with a high sensitivity accurate mass scan and a simultaneous data dependent scan. The honey samples were of different botanical origin: acacia (Robinia pseudoacacia), sunflower (Helianthus annuus), linden (Tilia cordata), basil (Ocimum basilicum), buckwheat (Fagopyrum esculentum), oilseed rape (Brassica napus), and goldenrod (Solidago virgaurea). The presence of 43 compounds, mainly flavonoids, was proven in all honey samples by their characteristic mass spectra and fragmentation pattern. Relatively high amounts of chrysin, pinocembrin and galangin were identified in all honey extracts. p-Coumaric acid was not detected in basil, buckwheat and goldenrod honey extracts. A larger amount of gallic acid (max value 1.45 mg/kg) was found in the sunflower honey, while a larger amount of apigenin (0.97 mg/kg) was determined in the buckwheat honey in comparison with other honeys. The samples were classified according to the botanical origin using pattern recognition technique, Principal Component Analysis (PCA). The LTQ OrbiTrap technique was proven to be reliable for the unambiguous detection of phenolic acids, their derivatives, and flavonoid aglycones based on their molecular masses and fragmentation pattern.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Food Chemistry", title = "The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry", volume = "138", number = "1", pages = "32-40", doi = "10.1016/j.foodchem.2012.10.025" }
Keckes, S., Gašić, U. M., Ćirković-Veličković, T., Milojković-Opsenica, D., Natić, M.,& Tešić, Ž. Lj.. (2013). The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry. in Food Chemistry Elsevier Sci Ltd, Oxford., 138(1), 32-40. https://doi.org/10.1016/j.foodchem.2012.10.025
Keckes S, Gašić UM, Ćirković-Veličković T, Milojković-Opsenica D, Natić M, Tešić ŽL. The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry. in Food Chemistry. 2013;138(1):32-40. doi:10.1016/j.foodchem.2012.10.025 .
Keckes, Silvio, Gašić, Uroš M., Ćirković-Veličković, Tanja, Milojković-Opsenica, Dušanka, Natić, Maja, Tešić, Živoslav Lj., "The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry" in Food Chemistry, 138, no. 1 (2013):32-40, https://doi.org/10.1016/j.foodchem.2012.10.025 . .