Suppression of the modulatory effects of the antileukemic and anti-human immunodeficiency virus compound avarol on gene expression by tryptophan
Abstract
The amino acid L-tryptophan is known to be a modulator of many processes of cell metabolism. In this contribution we show that L-tryptophan interferes with some biological effects of the antileukemic and anti-human immunodeficiency virus agent avarol, possibly by different mechanisms. Avarol has been shown to be able to modulate posttranscriptional events of mRNA synthesis, resulting in an increase of the base-sequence complexities of the nonabundant and rare mRNA classes. Here it is demonstrated that this change in mRNA abundancy distribution is accompanied by an increase in the level of some specific, low abundant mRNAs (ras and c-myc). Addition of L-tryptophan was found to abolish avarol-caused gene relaxation in 1,1210 mouse leukemia cells. In addition, L-tryptophan suppressed the induction of γ-interferon mRNA production in human peripheral blood lymphocytes. At the level of DNA, L-tryptophan inhibited the production of strand breaks by cytotoxic avarol concentrations in Friend er...ythroleukemia cells in vitro. Moreover, it competed with avarol for binding to the nuclear envelope binding site; this effect was not shown by other amino acids. © 1989, American Association for Cancer Research. All rights reserved.
Source:
Cancer Research, 1989, 49, 8, 2069-2076Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Schröder, H.C. AU - Wenger, R. AU - Gerner, H. AU - Reuter, P. AU - Kuchino, Y. AU - Sladić, Dušan AU - Mülle, W.E.G. PY - 1989 UR - https://cherry.chem.bg.ac.rs/handle/123456789/15 AB - The amino acid L-tryptophan is known to be a modulator of many processes of cell metabolism. In this contribution we show that L-tryptophan interferes with some biological effects of the antileukemic and anti-human immunodeficiency virus agent avarol, possibly by different mechanisms. Avarol has been shown to be able to modulate posttranscriptional events of mRNA synthesis, resulting in an increase of the base-sequence complexities of the nonabundant and rare mRNA classes. Here it is demonstrated that this change in mRNA abundancy distribution is accompanied by an increase in the level of some specific, low abundant mRNAs (ras and c-myc). Addition of L-tryptophan was found to abolish avarol-caused gene relaxation in 1,1210 mouse leukemia cells. In addition, L-tryptophan suppressed the induction of γ-interferon mRNA production in human peripheral blood lymphocytes. At the level of DNA, L-tryptophan inhibited the production of strand breaks by cytotoxic avarol concentrations in Friend erythroleukemia cells in vitro. Moreover, it competed with avarol for binding to the nuclear envelope binding site; this effect was not shown by other amino acids. © 1989, American Association for Cancer Research. All rights reserved. T2 - Cancer Research T1 - Suppression of the modulatory effects of the antileukemic and anti-human immunodeficiency virus compound avarol on gene expression by tryptophan VL - 49 IS - 8 SP - 2069 EP - 2076 UR - https://hdl.handle.net/21.15107/rcub_cherry_15 ER -
@article{ author = "Schröder, H.C. and Wenger, R. and Gerner, H. and Reuter, P. and Kuchino, Y. and Sladić, Dušan and Mülle, W.E.G.", year = "1989", abstract = "The amino acid L-tryptophan is known to be a modulator of many processes of cell metabolism. In this contribution we show that L-tryptophan interferes with some biological effects of the antileukemic and anti-human immunodeficiency virus agent avarol, possibly by different mechanisms. Avarol has been shown to be able to modulate posttranscriptional events of mRNA synthesis, resulting in an increase of the base-sequence complexities of the nonabundant and rare mRNA classes. Here it is demonstrated that this change in mRNA abundancy distribution is accompanied by an increase in the level of some specific, low abundant mRNAs (ras and c-myc). Addition of L-tryptophan was found to abolish avarol-caused gene relaxation in 1,1210 mouse leukemia cells. In addition, L-tryptophan suppressed the induction of γ-interferon mRNA production in human peripheral blood lymphocytes. At the level of DNA, L-tryptophan inhibited the production of strand breaks by cytotoxic avarol concentrations in Friend erythroleukemia cells in vitro. Moreover, it competed with avarol for binding to the nuclear envelope binding site; this effect was not shown by other amino acids. © 1989, American Association for Cancer Research. All rights reserved.", journal = "Cancer Research", title = "Suppression of the modulatory effects of the antileukemic and anti-human immunodeficiency virus compound avarol on gene expression by tryptophan", volume = "49", number = "8", pages = "2069-2076", url = "https://hdl.handle.net/21.15107/rcub_cherry_15" }
Schröder, H.C., Wenger, R., Gerner, H., Reuter, P., Kuchino, Y., Sladić, D.,& Mülle, W.E.G.. (1989). Suppression of the modulatory effects of the antileukemic and anti-human immunodeficiency virus compound avarol on gene expression by tryptophan. in Cancer Research, 49(8), 2069-2076. https://hdl.handle.net/21.15107/rcub_cherry_15
Schröder H, Wenger R, Gerner H, Reuter P, Kuchino Y, Sladić D, Mülle W. Suppression of the modulatory effects of the antileukemic and anti-human immunodeficiency virus compound avarol on gene expression by tryptophan. in Cancer Research. 1989;49(8):2069-2076. https://hdl.handle.net/21.15107/rcub_cherry_15 .
Schröder, H.C., Wenger, R., Gerner, H., Reuter, P., Kuchino, Y., Sladić, Dušan, Mülle, W.E.G., "Suppression of the modulatory effects of the antileukemic and anti-human immunodeficiency virus compound avarol on gene expression by tryptophan" in Cancer Research, 49, no. 8 (1989):2069-2076, https://hdl.handle.net/21.15107/rcub_cherry_15 .